Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil     

WU Xiao-hu  XU Jun  LIU Yong-zhuo  DONG Feng-shou  LIU Xin-gang  ZHANG Wen-wen  ZHENG Yong-quan 《中国农业科学(英文版)》2015,(1):114-124

The aim of this work was to assess the effect of applying three different doses of fluxapyroxad on microbial activity, community structure and functional diversity as measured by respiration, microbial biomass C, phospholipid fatty acid(PLFA) and community-level physiological profiles(CLPPs). Our results demonstrated that substrate-induced respiration(on day 15) and microbial biomass C(on days 7 and 15) were inhibited by fluxapyroxad, but stimulation was observed thereafter. In contrast, fluxapyroxad addition increased the basal respiration and metabolic quotients(q CO2) and respiratory quotients(QR). Analysis of the PLFA profiles revealed that the total and bacterial biomass(both Gram-positive bacteria(GP) and Gram-negative bacteria(GN)) were decreased within the initial 15 days, whereas those as well as the GN/GP ratio were increased at days 30 and 60. F luxapyroxad input decreased the fungi biomass but increased the bacteria/fungi ratio at all incubation time. Moreover, high fluxapyroxad input(75 mg fluxapyroxad kg–1 soil dry weight) increased the microbial stress level. A principal component analysis(PCA) of the PLFAs revealed that fluxapyroxad treatment s ignificantly shifted the microbial community structure, but all of the observed effects were transient. Biolog results showed that average well color development(AWCD) and functional diversity index(H′) were increased only on day 60. I n addition, the dissipation of fluxapyroxad was slow in soil, and the degradation half-lives varied from 158 to 385 days depending on the concentration tested.  相似文献   

园林机械（以割草机为代表）用改性PP材料开发与应用     

李军  朱海燕 《湖南农机》2011,(5):43-44

文章从PP材料的改性原理、园林机械用改性PP材料的性能要求出发,全面阐述了园林机械用改性PP材料的开发研制过程,开发出了一系列园林机械用改性PP材料并全面在ZS系列园林机械上得到了应用。  相似文献   

立式无筛饲料超微粉碎机的研发及质量检测分析     

葛一健  王君 《渔业现代化》2011,(5)

介绍了我国立式无筛饲料超微粉碎机的发展,简述立式无筛饲料超微粉碎机的组成、工作原理和优越性,分析质量检测及立式无筛饲料超微粉碎机技术的发展,展望制定行业标准的预期效果。  相似文献   

房山区中南部沟域景观资源分析及规划探讨     

李方园  赵和文  王学普  付军 《北京农学院学报》2015,30(1):88-92

本文从风景园林规划的基本目的入手,通过实践和调研房山区中南部沟域景观资源,分析地区内各景观体系类型,分类整合地区景观资源特征,挖掘房山中南部景观资源本身价值,并通过对房山区中南部地区进行上方花海沟域景观规划,系统研究房山区中南部沟域地区的景观建设发展方向,为深层综合开发、培育和保护当地景观资源提出改进措施,亦为房山区中南部的沟域经济的建设发展提供必要的理论依据。  相似文献   

产甲壳素酶菌株的筛选与诱变     

夏祥  吕梦圆  罗玲  蔡俊  胡瑛 《湖北农业科学》2015,54(5)

以胶体甲壳素作为惟一碳源,从自然界微生物中筛选并诱导到一株产甲壳素酶活性较高的菌株,经16S r DNA鉴定为Aeromonas sp.。并以该菌株作为出发菌株,经过一次紫外诱变,酶活是初筛菌株的1.48倍。再经过二次紫外诱变或者紫外-Li Cl复合诱变,酶活分别是初筛菌株的3.16和3.87倍,最高酶活达到3.48 U/m L。最后通过连续传代培养,确定了菌株HMX-16经过紫外和复合诱变后突变性状稳定性良好。  相似文献   

Effects of Addition of Three Types of Biochar on the Soil Nitrogen Balance and Cotton Yield   总被引：1，自引：1，他引：0  

Huang Ting  Ni Jieqiang  Xu wenxia  Zhai Yong  Ye Jun  Hou Zhen’an 《棉花学报》2015,27(6):595-600

The objective of this study was to determine the effect of biochar addition on the soil N balance and cotton yield in a drip-irrigated cotton field. The experiment included four soil amendments: control, cotton straw biochar, corn cob biochar, and poultry manure biochar. The biochar was applied at a rate of 4.5 t·hm^－2 except control. The results showed that all three types of biochar significantly reduced both the apparent soil N loss and the N surplus. The effect of cotton straw biochar treatment was most significant, relative to the control. Plant nitrogen uptake differed significantly between the different treatments, decreasing in the order cotton straw biochar > poultry manure biochar > corn cob biochar. Cotton yield also differed significantly between the different treatments, decreasing in the order poultry manure biochar > corn cob biochar, and cotton straw biochar.  相似文献   

京郊生态果园中的植物调查     

王文和  田晔林  冷平生 《北京农学院学报》2015,30(3):56-61

北京郊区生态果园数量较多。对不使用除草剂,主要采取清耕措施为主的生态果园中非靶标植物进行调查、分类和分析,将果园中植物分为4类,即果园杂草、果园行间生草和种草、果园趋避植物、果园栅栏植物。其中果园杂草有34科95属115种,其中常见的83种。常见种中以一、二年生草本和具地下根状茎或以地面芽越冬的多年生草本为主,彻底清除比较困难。并和30年前果园杂草从物种多样性和分布情况方面进行了比较,发现物种多样性有所下降,少了10科31属59种植物,但也新增加了2科17属30种,净减少8科14属29种;杂草中有7种属于外来入侵物种。统计出行间种草10种,种植趋避植物20种,建议用作栅栏植物8种。  相似文献   

废名新诗理论与中国“自然”诗学传统     

赵黎明 《湖南农业大学学报(自然科学版)》2011,(2):90-94

传统的经典理论分析了政府供给公共产品和消费者无差别消费时的经济问题。然而在市场经济中消费者的类型是不相同的，对公共产品的消费需求也各不相同。对此，需要研究不同消费需求、不同供给模式下具有“消费信号”的公共产品自愿供给问题。研究发现，提高公共产品消费的信号性，优化公共产品的供给模式对于公共产品的自愿供给非常重要。同时，识别出“信号消费”下的异质消费者，并使公共产品具有私人产品的消费特征时，公共产品自愿供给的意愿将会增加。  相似文献   

淮安区水稻测土配方施肥配方校正与示范对比试验报告     

成军 《北方水稻》2015,(1):35-36,59

为验证肥料配方的准确性,发挥配方肥料施用的最大效益。通过田间校验,示范对比测土配方施肥的增产效果,进一步验证并完善淮安区的肥料配方,优化测土配方施肥技术参数。  相似文献   

Effects of bFGF on brain edema,nerve function injury and autophagy related protein in rats with traumatic brain injury     

WANG Wen-hong  KONG Jun  LIN Xiao-xiang  LI Jian-xia  CHEN Lu-kui 《园艺学报》2019,35(2):320-325

AIM:To study the effects of basic fibroblast growth factor (bFGF) on brain edema, nerve function damage and autophagy related proteins in rats with head injury. METHODS:The rat model of craniocerebral injury (CI) was constructed. The rats were divided into control group, CI group, and low-, middle-and high-dose bFGF groups (n=10). The CI model was established in CI group, while the rats in control group were not given epidural impact. The rats in low-dose, middle-dose and high-dose bFGF groups were given bFGF at 2, 4 and 6 μg, respectively, by intraperitoneal injection after 30 min. The neurological function in the rats was evaluated by improved neurological function scoring. The rat brain tissues were taken, and the water content was detected. The levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β in the brain tissue were measured by ELISA. The malondialdehyde (MDA) content was analyzed by thiobarbituric acid method. The activity of superoxide dismutase (SOD) was examined by WST-8 assay. The glutathine peroxidase (GSH-Px) activity was detected by colorimetric method. The protein levels of autophagy related proteins LC3-Ⅱ and beclin-1 in the brain tissues were determined by Western blot. RESULTS:The neurological function score was increased significantly of the rats in CI group. The rat model of craniocerebral injury was successfully constructed. Neurological function scores in the rats in low-dose, middle-dose and high-dose bFGF groups were reduced, the water content of the brain tissue was also reduced (P<0.05). The levels of TNF-α, IL-6 and IL-1 β were decreased in the brain tissues (P<0.05), the content of MDA was declined (P<0.05), the activities of SOD and GSH-Px were increased (P<0.05), the protein levels of LC3-Ⅱ and beclin-1 were decreased, compared with the untreated rats in CI group (P<0.05). CONCLUSION:bFGF improves the nerve function of the rats with craniocerebral injury, reduces the water content of the brain tissue, reduces the expression of autophagic protein LC3-Ⅱ and beclin-1.The mechanism is related to the inhibition of inflammatory reaction and oxidative damage.  相似文献