Protective effects of taurine on LPS-induced myocardial damage in rats     

TANG Wen-tian  WANG Jing  LIU Jun  LU Xiao-hua  WANG Guo-guang  JIANG Yu-xin 《园艺学报》2017,33(1):123-127

AIM: To investigate the effects of taurine on lipopolysaccharide (LPS)-induced myocardial damage in rats. METHODS: Healthy male SD rats (n=30) were randomly divided into control group (CON), LPS model group (LPS) and taurine treatment group (TAU). The rats in CON group and LPS group were intravenously injected with normal saline, and the rats in TAU group were injected with taurine (100 mg/kg). After 2 h, the rats in LPS group and TAU group were intraperitoneally injected with LPS at 10 mg/kg, and the rats in CON group were injected with normal saline. Six hours after injection of LPS, the blood samples were collected for determination of superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, and tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) levels. The myocardial tissues were processed for histological examination and the analysis of Western blot. RESULTS: Compared with CON group, LPS significantly reduced SOD activity in the serum and heme oxygenase 1 (HO-1) protein expression in the myocardial tissues, increased the serum content of MDA and levels of TNF-α and IL-6. LPS also significantly elevated the levels of TNF-α and IL-6, and up-regulated the cyclooxygenase-2 (COX-2) expression and phosphorylation of nuclear factor kappa B (NF-κB) in the myocardial tissues. Taurine pretreatment significantly elevated SOD activity and HO-1 protein expression level, decreased the levels of COX-2, TNF-α, IL-6 and phosphorylated NF-κB. Histological observation showed that taurine reduced inflammatory response in the myocardial tissue. CONCLUSION: Taurine attenuates LPS-induced myocardial damage in rats. The beneficial effects of taurine may be associated with its reduction of p-NF-κB/COX-2 signaling by activation of HO-1/CO.  相似文献   

施氮深度对‘黄金梨’树氮素吸收、分配及利用效率的影响     

武阳  孙明德  刘军  田海青  王文娟  刘松忠 《园艺学报》2017,44(11):2171-2178

以7年生‘黄金梨’树为试材,采用~(15)N示踪技术研究了3个施氮深度[0(表面施氮)、20和40 cm]处理下不同器官对氮素的吸收、分配及利用效率,并探讨了氮素在土壤中的残留及损失。结果表明,梨果实成熟期,果实的Ndff值最高,其他器官的Ndff值均表现为20 cm施氮深度处理显著高于其他处理。各施氮深度处理,~(15)N的分配率均为贮藏器官最高,生殖器官最低。20 cm施氮深度处理的梨树N利用率最高(26.23%),40 cm施氮深度处理最低(15.65%)。N损失率以表面施氮处理最高(54.21%),20 cm施氮深度最低;0~80 cm土层的N残留率以40 cm施氮深度处理最高(31.73%),表面施氮处理最低。因此,本研究中,20 cm深度施氮处理能提高梨树各器官对N肥的吸收征调能力,氮素损失少。  相似文献   

菜用甘薯新品种薯绿1号的选育     

曹清河  季志仙  李强  王欣  唐君  赵冬兰  周志林  张安  戴习彬 《中国蔬菜》2017,1(3):70-72

薯绿1号(原名徐菜薯1号)是以台农71为母本,以广薯菜2号为父本,通过定向有性杂交选育而成的菜用甘薯专用新品种。株型半直立,分枝多,叶片心形,顶叶黄绿色,叶基色和茎色均为绿色。薯块纺锤形,白皮白肉。茎尖无茸毛,烫后颜色翠绿至绿色,无苦涩味,微甜,有滑腻感,食味好。高抗茎线虫病,抗蔓割病。在国家区域试验3个月时间内每667m2产量1800kg左右,如在保护地条件下可周年生产,每667m2产量在5000kg以上。适合江苏、山东、河南、浙江、四川、广东、福建、海南等适宜地区作叶菜种植。  相似文献   

甜玉米新品种‘浙甜12’     

吕桂华  郭国锦  吴振兴  陈坚剑  王建强  贾俊 《园艺学报》2017,44(Z2):2707-2708

 ‘浙甜12’是以自交系‘GA1101’为母本,‘GA218’为父本杂交育成的超甜玉米新品种。平均鲜果穗产量为11 556 ~ 14 782.5 kg · hm^-2,穗长19.8 ~ 21.0 cm,穗粗5.0 cm,单穗质量262.0 ~ 279.7 g,籽粒可溶性糖含量26.6%,适口性好,商品性佳。  相似文献   

Effect of tetrandrine on radiosensitivity of nasopharyngeal carcinoma cells     

WANG Jun  CHANG Li-hong  LI Xia  CHEN Xian-zhen  WU Xi-fu  WANG Zhi-yuan  HUANG Zi-zhen  HUANG Jian-cong  ZHANG Ge-hua 《园艺学报》2017,33(9):1611-1618

AIM: To investigate the mechanism of the radiosensitizing effect of maximum non-cytotoxic doses of tetrandrine (Tet) on nasopharyngeal carcinoma cell lines CNE1 and CNE2.METHODS: The cells were treated with ma-ximum non-cytotoxic doses of Tet (for CNE1 cells at 1.5 μmol/L and for CNE2 cells at 1.8 μmol/L), irradiation at 4 Gy, or combination of irradiation and maximum non-cytotoxic doses of Tet. The cell cycle distribution was analyzed by flow cytometry. The protein levels of γ-H2AX, cleaved caspase-3, p-CDC25C, CDK1, p-CDK1, cyclin B1, ERK and p-ERK were determined by Western blot.RESULTS: The expression of γ-H2AX was increased in CNE1 cells and CNE2 cells after combined treatment with irradiation and maximum non-cytotoxic doses of Tet. The percentages of CNE1 cells and CNE2 cells at G₂/M phase in irradiation group were (18.09±0.42)% and (18.48±1.32)%, respectively, which were decreased to (15.88±1.04)% and (13.80±0.82)% in combined treatment group, respectively (P<0.05). Combined treatment enhanced the increase in the protein level of cleaved caspase-3 caused by irradiation. The protein levels of p-CDC25C and p-CDK1 were increased in a dose-dependent manner by Tet treatment (P<0.05), while the expression of CDK1 showed no difference among different doses of Tet treatments. The protein levels of p-CDC25C, p-CDK1 and CDK1 showed no difference after the treatment with maximum non-cytotoxic doses of Tet. The combined treatment with irradiation and the maximum non-cytotoxic doses of Tet decreased the protein levels of p-CDC25C and p-CDK1 (P<0.05), increased the expression of cyclin B1, and had no influence on the expression of CDK1 (P<0.05). The combined treatment resulted in an increase in the protein level of p-ERK1 (P<0.05).CONCLUSION: The maximum non-cytotoxic doses of Tet enhance the DNA damage and apoptosis in CNE1 cells and CNE2 cells caused by irradiation, and the mechanism might be associated with ending of G₂/M arrest via activation of ERK/CDC25C/CDK1/cyclin B1 pathways.  相似文献   

Effect of GYY4137 on cytosolic lipid decomposition in mouse primary hepatocytes     

WANG Hong-gang  ZHANG You-jing  FU Chao-shen  WANG Jun  WU Dong-dong  ZHONG Pei-yu  WANG Guo-ying  LI Yan-zhang 《园艺学报》2017,33(8):1520-1523

AIM: To evaluate the effect of GYY4137, a novel hydrogen sulfide (H₂S) donor, on cytosolic lipid decomposition in mouse primary steatosis hepatocytes. METHODS: Oleic acid (OA) was used to induce hepatic steatosis model in vitro. The C57BL/6 mouse primary hepatocytes isolated and cultured by 2-step in situ perfusion were divided into 4 groups:the cells in control group were incubated with normal medium for 54 h; the cells in model group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 for 6 h; the cells in H₂S group or DL-propar-gylglycine (PAG; an inhibitor of cystathione γ-lysase, inhibiting H₂S synthesis) group were incubated with OA at 1.2 mmol/L for 48 h followed by serum-free phenol red-free RPMI-1640 which contained 1 mmol/L GYY4137 or 200 μmol/L PAG for 6 h. The glycerin release and the protein expression of hormone-sensitive lipase (HSL) in the cells were mea-sured. RESULTS: Compared with model group, the glycerin release and the protein expression of phosphorylated HSL (p-HSL) in H₂S group decreased significantly, while those increased significantly in PAG group. CONCLUSION: In steatosis hepatocytes, exogenous H₂S possibly decreases cytosolic lipid decomposition by decreasing the protein level of p-HSL.  相似文献   

辽宁马勃科大型真菌多样性(Ⅰ)     

马腾飞  高国平  王月  马骏 《北方园艺》2017,(5):142-144

于2014—2015年在辽宁抚顺、沈阳地区采集了马勃科大型真菌标本,鉴定该地共有马勃3属13种。其中长刺马勃(Lycoperdon echinatum Pers.)和光皮马勃(Lycoperdon glabrescens Berk.)为辽宁新纪录种,昏暗马勃(Lycoperdon nigrescens Pers.)为中国新纪录种。结合辽宁其他地区的马勃资料,统计辽宁共有马勃6属29种。  相似文献   

不同肥料配方对日光温室春茬番茄产量品质和养分吸收的影响   总被引：1，自引：0，他引：1  

廉晓娟  王艳  杨军  梁新书  张金良  王正祥 《北方园艺》2017,(4):39-42

以番茄品种"朝研299"为试材,研究日光温室滴灌条件下不同肥料配方对春茬番茄产量、品质和养分吸收的影响,从而确定适宜该区的最佳肥料配比。结果表明:氮磷钾追施比例(N∶P_2O_5∶K_2O)为1.0∶0.4∶1.8时,能够明显提高番茄产量,并增加番茄果实中维生素C、可溶性总糖含量,提高糖酸比,改善果实品质;且明显提高拉秧期植株干物质总积累量,干物质含量为果实茎叶根,该配比养分吸收总量最高,K2ONP_2O_5,根据产量和养分吸收含量计算,每生产1 000kg番茄需N 1.73kg,P_2O_50.89kg,K_2O 3.51kg,天津地区最适宜的春茬番茄种植追肥配方为1.0∶0.5∶2.0。  相似文献   

叶面喷施硝酸铵对两种黄瓜苗期生理指标的影响     

徐敏  程功  刘君  马倩倩 《北方园艺》2017,(8)

以广泛种植的2个黄瓜品种"长春密刺"和"水果黄瓜"为试材,叶面喷施0.30%、0.60%、0.90%硝酸铵,研究了不同浓度硝酸铵对幼苗鲜质量、叶绿素含量、渗透调节物质及酶活性等生理指标的影响,为有效且合理地施用氮肥提供参考依据。结果表明:喷施硝酸铵可提高2种黄瓜幼苗的鲜质量,对叶绿素a含量影响不大,促进叶绿素b含量和叶绿素总量的增加;随着喷施硝酸铵浓度的增大,可提高2种黄瓜的游离脯氨酸含量,降低可溶性糖含量,对可溶性蛋白质含量影响不明显;随着喷施硝酸铵浓度的增大,2种黄瓜的超氧化物歧化酶(SOD)活性变化趋势均为先增后降,而过氧化氢酶(CAT)活性变化趋势则呈现出先降后升,但较对照差异均不显著;"长春密刺"和"水果黄瓜"的叶面喷施硝酸铵最适浓度分别是0.30%和0.60%。  相似文献   

收获期花椒的力学特性与形态特征测定与分析     

冯亚利  彭俊  孙世鹏  傅隆生 《农机化研究》2017,(7):43-47

我国花椒的收获主要以人工收获为主。随着社会的发展,花椒市场需求不断扩大,迫切需要解决花椒收获效率低下的问题。因此,花椒收获机械化已成为椒农的心声,研究分析收获期花椒的力学特性可以为花椒收获机械的设计者提供必须的基础理论数据。因花椒在品种、气候条件、土壤及其他方面的差异,其力学特性会有较大的差别。为此,通过陕西省韩城市收获期花椒的实验室测定,采用图像解析和数理统计方法对收获期花椒的力学特性进行分析,得出了花椒主要几何特性指标值的变化区间,并统计分析了花椒果实与果柄及果柄与树枝之间的分离力以及花椒果实的颜色特征,为花椒的收获和加工机器系统的开发提供科学依据。  相似文献