捻转血矛线虫离体培养的研究   总被引：2，自引：0，他引：2  

孟雁  孔繁瑶 《畜牧兽医学报》1993,24(1):74-80

本实验研究了:(1)三种人工合成培养基:M199、NCTC109、F_(12);(2)小牛脱纤维蛋白血(以下简称小牛脱纤血)、Filde’s试剂,Bovine hemin;(3)pH值等几种因素对离体培养中捻转血矛线虫(Haemonchou contortus)L_3发育至L_4的影响。结果表明:(1)在相同条件下,NCTC109和M199培养系中的L_3可发育至L_4晚期,F_(12)中L_3只发育至L_4早期;三种培养系中L_3发育至L_4的虫体数占存活虫体数的90％以上,以NCTC109中的发育率为最高,达100％。(2)小牛脱纤血、Filde’s试剂和Bovine hemin对L_3发育至L_4均有促进作用,虫体的存活率明显提高,晚四期幼虫的存活时间延长;小牛脱纤血和Filde’s试剂可使L_3至晚L_4的时间缩短。(3)pH 5.8～6.2时,L_3发育至L_4;速度明显减慢,发育率不到50％;pH 6.6～6.8时,L_3发育至L_4的同步性及发育率明显提高,以pH6.6为最佳。  相似文献   

原条检验方法的探讨   总被引：1，自引：0，他引：1  

黄立波  门志平  高启彬  毕德军  王伟 《森林工程》2001,17(4):24-24,26

山河屯林业局用改造后弓形尺即能量原条长级，又能量径级，提高了工作效率。本文同时对原木检验方法进行了探讨。  相似文献   

3种环境友好型药剂对西花蓟马的室内毒力与田间防效   总被引：3，自引：0，他引：3  

张安盛  张思聪  李丽莉  门兴元  周仙红  于毅 《植物保护》2012,38(4):175-177

测定了3种杀虫剂对西花蓟马的室内毒力和田间防效。室内生物测定结果表明,多杀菌素、甲氨基阿维菌素苯甲酸盐、阿维菌素对西花蓟马成虫的LC50值为0.050～2.887mg/L,对西花蓟马若虫的LC50值为0.040～0.457mg/L;田间药效试验表明,田间防治西花蓟马时推荐使用剂量(防效>80%)分别为:2.5%多杀菌素悬浮剂30～60g/667m2,1%甲氨基阿维菌素苯甲酸盐乳油30～60g/667m2,1.8%阿维菌素乳油不可低于75g/667m2。  相似文献   

Pharmacokinetics of methylprednisolone acetate after intra-articular administration and subsequent suppression of endogenous hydrocortisone secretion in exercising horses     

MI Menéndez  MA Phelps  EA Hothem  AL Bertone 《American journal of veterinary research》2012,73(9):1453-1461

Objective-To determine the pharmacokinetics of methylprednisolone (MP) and the relationship between MP and hydrocortisone (HYD) concentrations in plasma and urine after intra-articular (IA) administration of 100 or 200 mg of MP acetate (MPA) to horses. Animals-Five 3-year-old Thoroughbred mares. Procedures-Horses exercised on a treadmill 3 times/wk during the study. Horses received 100 mg of MPA IA, then 8 weeks later received 200 mg of MPA IA. Plasma and urine samples were obtained at various times for 8 weeks after horses received each dose of MPA; concentrations of MP and HYD were determined. Pharmacokinetic-pharmacodynamic estimates for noncompartmental and compartmental parameters were determined. Results-Maximum concentration of MP in plasma was similar for each MPA dose; concentrations remained greater than the lower limit of quantitation for 18 and 7 days after IA administration of 200 and 100 mg of MPA, respectively. Maximum concentration and area under the observed concentration-time curve for MP in urine were significantly higher (approximately 10-and 17-fold, respectively) after administration of 200 versus 100 mg of MPA. Hydrocortisone concentration was below quantifiable limits for ≥ 48 hours in plasma and urine of all horses after administration of each MPA dose. Conclusions and Clinical Relevance-Pharmacokinetics of MP may differ among IA MPA dosing protocols, and MP may be detected in plasma and urine for a longer time than previously reported. This information may aid veterinarians treating sport horses. Further research is warranted to determine whether plasma HYD concentration can aid identification of horses that received exogenous glucocorticoids.  相似文献   

红锥扦插繁殖试验   总被引：13，自引：8，他引：5  

邓燕忠  连辉明 《广东林业科技》2005,21(3):45-48

红锥是我省重要的乡土阔叶树种,也是优良的用材树种。从2002-2004年设置了生根促进剂、容器、扦插时间及处理穗条的扦插比较试验,结果表明:红锥扦插生产宜在夏秋两季开展;选用半木质化穗条,用黄心土作基质,以5 cm×12 cm白色塑料袋作容器;穗条扦插前采用10%酒精溶液浸泡处理可获得较高的成活率。但激素对成活率的影响还需进一步开展研究。  相似文献   

Benefits of Daily Intake Tea on Human Health     

Qing Men  Guo-Liang Jie  Guang-Wei Wang  You-Ying Tu 《茶叶》2013,(4):435-441

The recent research achievements about the mechanisms of beneficial health effects of tea polyphenols and the epidemiological investigation on anti-cancer,anti-cardiovascular diseases,and anti-obesity through tea consumption were reviewed.It is proved that reactive oxygen species is the main reason to induce cell mutation,DNA damage,obesity,high blood pressure and many kinds of cancers.Tea catechins and theaflavins have excellent bioactivity on scavenging ROS due to their characteristic structure feature of phenolic hydroxyl group,which means drinking tea could prevent diseases.Epidemidogical investigations also showed that tea consumption could reduce the risk of lung cancer,gastric cancer,bladder cancer,and so on.However,the frequency of tea drinking or tea consumption amounts significantly affected the prevention efficiency.The epidemiologic investigations also find that drink green tea,especial black tea is useful to modify the cardiovascular diseases.In vivo and vitro tests showed that tea supplement could moderate the metabolism of blood lipid and prevent obesity.These indicated that tea and tea extracts might reduce the risk of many diseases.  相似文献   

高产、高出仁率花生新品种漯花10号的选育     

周彦忠  张宏套  李斯佳  姬小玲  李飞  乔新敏  胡文静 《作物研究》2019,(1):23-25,35

为适应我国长江流域花生生产的需要,以中花10号为母本,与自选品系漯河紫皮杂交,用一粒传法结合南繁加代选育出高产、高出仁率花生新品种漯花10号。介绍了该品种的选育过程及其区域试验与生产试验的结果:两年区域试验平均荚果产量4645. 5 kg/hm^2,比对照增产2. 32%,籽仁产量3513. 0 kg/hm^2,比对照增产6. 73%;生产试验平均荚果产量4125. 15 kg/hm^2,比对照增产7. 96%,籽仁产量3118. 5 kg/hm^2,比对照增产12. 48%。  相似文献   

成都平原西部土壤速效钾含量剖面分布特征及其影响因素   总被引：3，自引：0，他引：3  

董琴  王昌全  李启权  李冰  黎国阳  徐强  李萌  曾杰熙 《水土保持学报》2019,33(2):176-182

为了准确获取土壤钾素剖面分布信息,掌握区域钾素运移和合理精准施肥的需要,基于134个剖面(0—100 cm)采样数据,运用经典统计学和地统计学方法探讨了成都平原西部土壤速效钾剖面分布特征,并对比分析了成土母质、土壤类型和土地利用方式对土壤速效钾剖面分布的影响。结果表明:土壤速效钾含量在水平方向上具有一定的规律性,其高值密集在金马河以南的崇州、邛崃和大邑区域,而低值出现在都江堰—郫县—温江一带,低值区面积占研究区面积50%以上,总体呈现由西南部向东北部显著降低趋势。垂直方向上0—100 cm随土层逐渐降低,表层土壤显著高于下层土壤(P0.05),下层土壤变幅(最大值与最小值之差)随土壤深度的增加逐渐减小。0—100 cm不同土层块金系数均为25%～75%,表现为中等程度空间自相关性,受结构性因素和随机因素共同影响。表层土壤速效钾空间分布受成土母质、土壤类型和土地利用方式共同影响,土壤类型的解释能力最高,分别是成土母质和土地利用方式的1.97,2.58倍;下层土壤主要受成土母质和土地利用方式的影响,影响程度均随着土层深度的增加而增加,相较之下,母质的影响程度增加更为显著。总体来看,成土母质和土地利用方式是影响研究区土壤速效钾剖面分布的主控因素。  相似文献   

Relationships Among the Feeding Behaviors of a Mirid Bug on Cotton Leaves of Different Ages and Plant Biochemical Substances     

Haiyan Song  Zhaoke Dong  Lili Li  Zengbin Lu  Chao Li  Yi Yu  Xingyuan Men 《Journal of insect science (Online)》2021,21(1)

Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) tends to feed on young plant tissues. To explore the relationship between stylet probing behaviors of adult A. lucorum and conditions of cotton leaves, we conducted an experiment using electropenetrography (EPG). Behaviors were recorded on four cotton varieties, in relation to thickness and biochemical traits of differently-aged leaves. Cotton leaf age had a significant effect on the probing behavior of A. lucorum but cotton variety did not. One-day-old leaves of A. lucorum received the highest mean number of stylet probes (penetrations) per insect, and longest mean durations per insect of combined stylet probing or its components, cell rupture and ingestion behaviors. All of the leaf traits (thickness and biochemical substances) were similar among these four cotton varieties. Leaf thickness had a significantly negative effect on the same four variables above. Gossypol and tannin also had a negative impact on combined probing duration. Redundancy analysis showed that the four EPG variables were closely related to nutrient substances (amino acids, sugar, and water) while they had the opposite relationship with plant defense substances (gossypol and tannin). On cotton in the seedling stages, A. lucorum fed more readily on the youngest, thinnest leaves in our no-choice EPG experiments. Nutrients and chemical resistance substances determined the probing duration of A. lucorum. Our findings can contribute to better understanding of patterns of feeding and host consumption by A. lucorum, ultimately improving cotton resistance to A. lucorum.  相似文献   

Multi-trait and random regression approaches for addressing the wide range of weaning ages in Asturiana de los Valles beef cattle for genetic parameter estimation     

Menéndez-Buxadera A  Carleos C  Baro JA  Villa A  Cañón J 《Journal of animal science》2008,86(2):278-286

Weaning weight (WW) records of 24,066 Asturiana de los Valles beef cattle, including the progeny of 557 sires and 10,653 dams, were analyzed using a multitrait animal model (MAM) and a random regression model (RRM) in order to estimate the variance components and the breeding value of the animals. Three definitions of WW were used: early weaning (EW) for animals weaned before the age of 180 d; standard weaning (SW) for animals weaned between 180 and 240 d old; and late weaning (LW) for animals weaned between the ages of 240 and 365 d. The heritabilities (h(2)) were high (from 0.49 to 0.63), which fully agrees with previous estimates for this breed. The genetic correlations between EW and SW were 0.86, and lower between EW and LW at 0.543 to 0.622, using MAM and RRM models, respectively. Ranking of sires displayed changes depending on age at weaning of their offspring, which could explain the modest genetic progress reached using MAM evaluation. The first 2 eigenvalues of the random regression coefficient matrix explained 66 and 30% of the genetic variance, which implies important genetic variation underlying the form of the growth curve of the animals during the weaning period. The evaluation of sires according to the official method, as currently carried out by the breeders' association (WW adjusted previously to the age of 180 d), does not exploit the genetic differences in response to their production system where the calf is weaned at variable ages.  相似文献