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31.
RAPD and SSR markers were used for genetic diversity evaluations among 15 genotypes selected from the genus Prunus L. Altogether 40 RAPD primers and 21 primer pairs designated for microsatellite loci were applied on the whole group of genotypes. 相似文献
32.
Miroslav Glasa Véronique Marie-Jeanne Gérard Labonne Zdeno Šubr Otakar Kúdela Jean-Bernard Quiot 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(9):843-853
The isolate BOR-3, collected in Slovakia in 1996, was recently identified as a natural recombinant between an M and D type of Plum pox virus (PPV). Biological assays demonstrated its capacity to be aphid- and graft-transmitted to various Prunus spp. hosts. A study was carried out to determine the further presence of PPV recombinants in two epidemiologically distinct areas – Slovakia and France. Tools based on PPV-M and D subgroup typing, targeting P3–6K1, CI and CP regions of the PPV genome were used for recombinant identification. Closely related recombinant variants were detected in different Prunus spp. during a survey conducted in Slovakia in 2001, but not within a set of selected PPV isolates from France collected between 1985 and 2001. Sequence analysis of the (Cter)NIb–(Nter)CP region of 10 recombinant isolates from Slovakia showed their high homology, reaching more than 98%. All the recombinant isolates shared the same recombination breakpoint situated in the C terminus of the NIb gene. Our study demonstrates that the PPV recombinants are viable and competitive with conventional PPV-M and D isolates. The present work indicates that the occurrence of recombinants within PPV isolates might be more common than previously assumed. 相似文献
33.
Gazdik Filip Penazova Eliska Cechova Jana Baranek Miroslav Eichmeier Ales 《植物病害和植物保护杂志》2019,126(6):517-528
Journal of Plant Diseases and Protection - Pseudomonas amygdali pv. lachrymans (Pal) is a bacterium that causes angular leaf spot disease of cucumbers. There is no direct protection against this... 相似文献
34.
Martin Mikoláš Martin Tejkal Tobias Kuemmerle Patrick Griffiths Miroslav Svoboda Tomáš Hlásny Pedro J. Leitão Robert C. Morrissey 《Landscape Ecology》2017,32(1):163-179
Context
Distribution and connectivity of suitable habitat for species of conservation concern is critical for effective conservation planning. Capercaillie (Tetrao urogallus), an umbrella species for biodiversity conservation, is increasingly threatened because of habitat loss and fragmentation.Objective
We assessed the impact of drastic changes in forest management in the Carpathian Mountains, a major stronghold of capercaillie in Europe, on habitat distribution and connectivity.Methods
We used field data surveys with a forest disturbance dataset for 1985–2010 to map habitat suitability, and we used graph theory to analyse habitat connectivity.Results
Climate, topography, forest proportion and fragmentation, and the distance to roads and settlements best identified capercaillie presence. Suitable habitat area was 7510 km2 in 1985; by 2010, clear-cutting had reduced that area by 1110 km2. More suitable habitat was lost inside protected areas (571 km2) than outside (413 km2). Habitat loss of 15 % reduced functional connectivity by 33 % since 1985.Conclusions
Forest management, particularly large-scale clear-cutting and salvage logging, have substantially diminished and fragmented suitable capercaillie habitat, regardless of the status of forest protection. Consequently, larger areas with suitable habitat are now isolated and many patches are too small to sustain viable populations. Given that protection of capercaillie habitat would benefit many other species, including old-growth specialists and large carnivores, conservation actions to halt the loss of capercaillie habitat is urgently needed. We recommend adopting policies to protect natural forests, limiting large-scale clear-cutting and salvage logging, implementing ecological forestry, and restricting road building to reduce forest fragmentation.35.
36.
Jitka Hamáčková Andrea Lepičová Miroslav Prokeš Pavel Lepič Pavel Kozák Tomáš Policar Leon Andrzej Stanny 《Aquaculture International》2007,15(3-4):255-265
The ide Leuciscus idus (Linnaeus 1758) belongs to autochthonous, less frequent and vulnerable fish species in the frame of ichthyofauna of the Czech
Republic. It was the reason for testing the possibility of captive breeding and production of fry in a controlled environment.
The goal of this paper was to find the optimal transfer time of ide larvae from live food (Artemia salina nauplii) to dry starter feed providing good survival and growth. At the end of the experiment, high values of cumulative
survival (in percentages) and individual weight (in milligrammes) were found for ide fry in groups D (90.6%, 178.6 mg; 15 days
A.
salina + 10 days starter feed), A (89.9%, 169.9 mg; A. salina only) and C (88.6%, 160.4 mg; 10 days A. salina + 15 days starter feed). Significantly lower values of the given parameters were found in group B (57.1%, 92.6 mg; 5 days
A. salina + 20 days starter feed), and the lowest ones were in group E (29.8%, 75.6 mg; starter feed only). Mean values of the level
of ontogenesis at the end of the experiment in fry of A–E groups were analogous to length and weight growth. Groups D, C and
A consisted of juveniles only (J1, J2), and groups B and E consisted of larvae and juveniles (L4, L5, L6, J1, J2). Condition
and production parameters [Fulton’s coefficient of condition (FWC), total length (TL), weight (w), specific growth rate (SGR),
food conversion ratio (FCR), cumulative survival] and relationships between basic lengths TL, fork length (FL) and standard
length (SL) were evaluated. 相似文献
37.
Marssoniella elegans Lemmermann, 1900, a parasite of ovarial tissues of the copepod Cyclops vicinus Uljanin, 1875, was studied as a representative of aquatic-clade microsporidia which form "heteroinfectious spores" (spores not infective to the original host as opposed to "homoinfectious spores" which are infective for the original host) and which thus should require an alternate host. Several structural characters of this microsporidian are similar to those of copepod morphs of microsporidia infecting mosquitoes. However, small subunit ribosomal DNA phylogeny indicates that caddis flies (Insecta, Trichoptera) might be the alternate hosts of Marssoniella. Ultrastructural data obtained are used to redefine the genus Marssoniella Lemmermann, 1900 and its type species Marssoniella elegans. 相似文献
38.
Bjedov I Tenaillon O Gérard B Souza V Denamur E Radman M Taddei F Matic I 《Science (New York, N.Y.)》2003,300(5624):1404-1409
The evolutionary significance of stress-induced mutagenesis was evaluated by studying mutagenesis in aging colonies (MAC) of Escherichia coli natural isolates. A large fraction of isolates exhibited a strong MAC, and the high MAC variability reflected the diversity of selective pressures in ecological niches. MAC depends on starvation, oxygen, and RpoS and adenosine 3',5'-monophosphate regulons; thus it may be a by-product of genetic strategies for improving survival under stress. MAC could also be selected through beneficial mutations that it generates, as shown by computer modeling and the patterns of stress-inducible and constitutive mutagenesis. We suggest that irrespective of the causes of their emergence, stress-induced mutations participate in adaptive evolution. 相似文献
39.
Milisavljević MDj Timotijević GS Radović SR Brkljacić JM Konstantinović MM Maksimović VR 《Journal of agricultural and food chemistry》2004,52(16):5258-5262
An 8S storage globulin from buckwheat seed, which resembles the structure and features common to the vicilin-like family of seed storage proteins, was analyzed for this paper. It was found that expression of the 8S globulin gene precedes that of the 13S globulin (the main buckwheat storage protein) and starts from an early stage of buckwheat seed development (9-11 days after flowering), continuing to accumulate throughout seed development to contribute approximately 7% of total seed proteins. This protein fraction might be more interesting for biotechnological application than the 13S buckwheat legumin consisting of 23-25 kDa subunits reported to be the major buckwheat allergen. A partial cDNA was also isolated, showing high homology with cDNAs coding for vicilin-like storage proteins from various plant species, and its expression profile throughout seed development as well as in different buckwheat tissues was analyzed. 相似文献
40.