Defective Movement of Viruses in the Family Bromoviridae Is Differentially Complemented in Nicotiana benthamiana Expressing Tobamovirus or Dianthovirus Movement Proteins     

Rao AL  Cooper B  Deom CM 《Phytopathology》1998,88(7):666-672

ABSTRACT Taxonomically distinct tobacco mosaic tobamovirus (TMV), red clover necrotic mosaic dianthovirus (RCNMV), cucumber mosaic cucumovirus (CMV), brome mosaic bromovirus (BMV), and cowpea chlorotic mottle bromovirus (CCMV) exhibit differences in their host range. Each of these viruses encodes a functionally similar nonstructural movement protein (MP) that is essential for cell-to-cell movement of a progeny virus. Despite the lack of significant amino acid identity among the MPs of CMV, TMV, and RCNMV, movement-defective CMV (CMVFnyDeltaMP-DeltaKPN) was able to move locally and systemically in transgenic Nicotiana benthamiana expressing either TMV MP (NB-TMV-MP(+)) or RCNMV MP (NB-RCNMV-MP(+)). These observations contrast with those of previous studies in which transgenic N. tabacum cv. Xanthi plants expressing TMV MP supported only the cell-to-cell movement of CMVFnyDeltaMP-DeltaKPN. To verify whether similar complementation could be observed for movement-defective bromoviruses, NB-TMV-MP(+) and NB-RCNMV-MP(+) plants were inoculated independently with movement-defective variants of BMV (B3DeltaMP) and CCMV (CC3DeltaMP). Neither NB-TMV-MP(+) nor NB-RCNMV-MP(+) was able to rescue the defective cell-to-cell and long-distance movement of B3DeltaMP. In contrast, NB-RCNMV-MP(+) complemented the cell-to-cell, but not the long-distance, movement of CC3DeltaMP. Taken together, these studies suggest that virus movement is a complex process and that, in some cases, the host species plays a major role in determining the long-distance movement function of a virus.  相似文献   

农机推广培训工作中存在的问题及解决措施     

马龙武  陈海涛 《农业机械化与电气化》2009,(5):85-86

农机推广培训是农机工作的重要环节,但目前依然存在农机购置补贴政策实施滞后、农机产品供货不及时、农机购置补贴范围不宽、农机培训内容单一以及部分农机具定价偏高、适用性不强等问题,为此,提出牢固树立为农民服务的意识、合理分配农机化投入资金、建设高素质农机科技队伍、简化办事程序等工作措施,以促进农机推广培训工作的有效开展。  相似文献   

Responses of buffaloes to heavy working load under tropical conditions     

R.C. Upadhyay  M.V.N. Rao 《Livestock Production Science》1985,13(2):199-203

Four male buffaloes of 3 to $\text{3}\text{1}\text{2}$ years were subjected to heavy loads during hot dry conditions for 3 h continuously. Rectal temperature, respiration rate and heart rate increased significantly. The blood pH, P_O2, increased significantly and P_CO2 declined. During work, buffaloes were not able to eliminate heat produced and exhibited such distress symptoms as profuse salivation, frothing, lack of leg co-ordination, excitement and tongue protrusion. The muzzle secretion of buffaloes increased greatly. Even 3 h after discontinuation of work, the physiological reactions had not fully disappeared.  相似文献   

Influence of sources and doses of N and K on herbage,oil yield and nutrient uptake of patchouli [Pogostemon cablin (Blanco) Benth.] in semi-arid tropics     

Munnu Singh  R.S. Ganesha Rao 《Industrial Crops and Products》2009,29(1):229-234

Patchouli oil is one of the most important essential oils used in modern perfumery and cosmetic industries. There is hardly any preparation of oriental nature where patchouli oil is not used. It is used mainly because of fixative property as it gives tenacity to other perfumes. Field experiments were conducted at Bangalore, India, to study the influence of sources and doses of N and K on herbage, oil yield, nutrient uptake, nitrogen utilization efficiency and oil content of patchouli [Pogostemon cablin (Blanco) Benth.]. The results revealed that application of 200 kg N/ha and 41.5 kg K/ha produced significant higher patchouli herbage and oil yields compared with controls. Similarly, N and K uptake were also higher at 200 kg N and 41.5 kg K/(ha year) compared with controls. DCD-coated urea produced higher herbage, oil yield and N uptake and utilization efficiency compared with prilled urea. There was no effect of sources of K on the yield of patchouli. The oil content was not influenced by N, K doses or sources applied. N and K depletion were noticed in the soil.  相似文献   

海拨高度与肉鸡腹水征的相关性研究     

饶家荣  李权 《中国家禽》1999,(2)

选择云南省海拔高度76．4m～3200m不同的5个地、县自然发生的肉鸡腹水综合征（ASB）与同品种的健康鸡进行对比研究。结果证实，同品种肉鸡喂同种饲料并在相同的饲养管理条件下，无论在低海拔地区还是高海拔地区发病相同，肉鸡腹水综合征的发生与海拔高低无关。  相似文献   

计算法测量电阻应变原理的试验分析     

饶秀勤  吴国桢 《浙江大学学报(农业与生命科学版)》1998,(3)

介绍了计算法测量电阻应变的原理及试验，着重分析了试验结果，验证了测量原理．  相似文献   

Study on Mesoporous Silica Materials for Separation of Functional Food Ingredients     

Liu Feng  Jin Haitao  Ren Hongbo  Chen Guoyou  Meng Li 《农产品加工．学刊》2008,(7)

  相似文献   

影响犊牛瘤胃发育的因素研究     

张海涛  王加启  卜登攀  周凌云  魏宏阳 《乳业科学与技术》2008,31(2):86-89

瘤胃发育是犊牛生长发育的重要指标.论述瘤胃的组织形态学发育,通过对瘤胃上皮乳头长度、宽度、颜色、密度,瘤胃壁及其肌肉层厚度等相关性状的描述,探讨了犊牛日龄、饲料组成及其形态、挥发性脂肪酸,瘤胃pH值和瘤胃微生物等各种因素对犊牛瘤胃发育的影响.  相似文献   

不同播期对冬小麦粒重形成及产量的影响     

张学品  高海涛  段国辉  吴少辉  杨洪强  冯伟森  牛鑫 《勤云标准版测试》2007,(12)

以4个冬小麦品种为材料,研究了不同播期条件下籽粒灌浆特征参数、粒重和产量的差异。结果表明:播期对千粒重的影响存在差异,洛旱6号、洛麦21号和豫麦49不同播期之间的籽粒千粒重存在显著差异,但播期对偃展4110的千粒重影响不大。在不同播期下,灌浆过程中平均灌浆速率、快增期灌浆速率对粒重的影响较大。播期对不同品种灌浆高峰出现的日期影响基本一致,早播期>晚播期>中播期.但较早播种时,洛旱6号、洛麦21号和豫麦49灌浆高峰出现较早,灌浆速率大,晚播时偃展4110灌浆高峰出现较早,灌浆速率大。播期对4个品种产量的影响均以中播最高,在河南旱区生态条件下,似偃展4110的春性品种适宜播期为10月中下旬,洛旱6号、洛麦21号等似豫麦49的半冬性品种适宜播期为10月中旬。  相似文献   

Interaction between Eimeria uzura infection and aflatoxicosis in Japanese quail (Coturnix coturnix japonica)     

J R Rao  N N Sharma  P K Iyer  A K Sharma 《Veterinary parasitology》1990,35(3):259-267

The clinical signs and gross lesions caused by Eimeria uzura (10(5) oocysts) in Japanese quail (Coturnix coturnix japonica) exhibited little or no influence in the face of intercurrent dietary aflatoxicosis (1 p.p.m. of aflatoxin B1 from Day 0 to 55). Similarly, no significant differences in the mucosal morphology of the intestine were evident histologically between the two groups of Japanese quail. The nervous signs of ataxia, leg weakness, incoordination of movement, torticollis and terminal opisthotonos were toxin-induced manifestations. In the aflatoxic quail, hypoplastic changes and selective depletion of lymphocytes were more prominent in the bursa of fabricius. Increased relative mean weights of liver, kidney, spleen, crop, proventriculus and gizzard were observed in birds due to aflatoxin sensitivity. The combination of E. uzura infection and aflatoxicosis in Japanese quail may cause significant weight loss, and increased oocyst production and reproductive potential.  相似文献