Identification of the tet(B) resistance gene in Streptococcus suis     

Yogesh Chander  Simone R. Oliveira  Sagar M. Goyal 《Veterinary journal (London, England : 1997)》2011,189(3):359-360

The tetracycline resistance gene, tet(B), has been described previously in Gram negative bacteria. In this study tet(B) was detected in plasmid extracts from 17/111 (15%) Streptococcus suis isolates from diseased pigs, representing the first report of this resistance gene in Gram positive bacteria.  相似文献   

Convergence of developmental mutants into a single tomato model system: 'Micro-Tom' as an effective toolkit for plant development research     

Carvalho RF  Campos ML  Pino LE  Crestana SL  Zsögön A  Lima JE  Benedito VA  Peres LE 《Plant methods》2011,7(1):18-14

Background

The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the great diversity of tomato cultivars used by the research community, it is often difficult to reliably compare phenotypes. The lack of tomato developmental mutants in a single genetic background prevents the stacking of mutations to facilitate analysis of double and multiple mutants, often required for elucidating developmental pathways.

Results

We took advantage of the small size and rapid life cycle of the tomato cultivar Micro-Tom (MT) to create near-isogenic lines (NILs) by introgressing a suite of hormonal and photomorphogenetic mutations (altered sensitivity or endogenous levels of auxin, ethylene, abscisic acid, gibberellin, brassinosteroid, and light response) into this genetic background. To demonstrate the usefulness of this collection, we compared developmental traits between the produced NILs. All expected mutant phenotypes were expressed in the NILs. We also created NILs harboring the wild type alleles for dwarf, self-pruning and uniform fruit, which are mutations characteristic of MT. This amplified both the applications of the mutant collection presented here and of MT as a genetic model system.

Conclusions

The community resource presented here is a useful toolkit for plant research, particularly for future studies in plant development, which will require the simultaneous observation of the effect of various hormones, signaling pathways and crosstalk.  相似文献   

Fatty acid pattern, oxidation product development, and antioxidant loss in muscle tissue of rainbow trout and Dicentrarchus labrax during growth     

Passi S  Ricci R  Cataudella S  Ferrante I  De Simone F  Rastrelli L 《Journal of agricultural and food chemistry》2004,52(9):2587-2592

The levels of hydrophilic, lipophilic, and enzymatic antioxidants, the oxidative damage to lipids and proteins, and the fatty acid patterns of triglyceride and phospholipid fractions were assayed in fresh muscle tissue of rainbow trouts (Oncorhynchus mykiss) and sea basses (Dicentrarchus labrax) during aging, to investigate the correlation between oxidative stress and aging processes in fish. The present studies suggests that lipid peroxidation and accumulation of oxidized proteins during in vivo aging are most likely to be linked with an age-dependent decline of lipophilic antioxidants (CoQH(2), CoQ, and vitamin E) and vitamin C contents in muscle tissue, whereas fish aging is not linked to a decline in antioxidant enzymes and reduced glutathione levels. Lipophilic antioxidant and vitamin C levels represent a reliable marker of oxidative stress during aging, and their determination might be useful for the assessment of fish age.  相似文献   

Lessons about extinction and translocation: models for eastern barred bandicoots (Perameles gunnii) at Woodlands Historic Park, Victoria, Australia     

Charles R. Todd  Simone JenkinsAndrew R. Bearlin 《Biological conservation》2002,106(2):211-223

In 1998 the protected population of Perameles gunnii (eastern barred bandicoot) at Woodlands Historic Park, Victoria, Australia became functionally extinct following removals of individuals for translocation between 1994 and 1996. Population viability analysis and data collected whilst the population was in decline are used to explore the impact that these removals had on the decline of the P. gunnii population at Woodlands. Due to some ambiguities in the life history of P. gunnii at Woodlands, two structurally different population models were used. Explicitly including the removal of animals in the analysis indicates that there may be at least a three-fold increase in the risk of quasiextinction due to removals. In some scenarios, over 17% of the trajectories decrease to a level less than or equal to 10 females within the observed time period of the collapse of the population, that is by April 1998. The modelling approach identified a number of critical factors in the decline of the population, such as variation in survival rates. By representing the life history strategy using two alternate models, quantitative statements about the impact that removals had on the population decline at Woodlands are made. While removals probably contributed to the collapse of the population, removals were not the sole cause of population decline. The real reasons for decline remain unknown; however, it is likely to be closely linked to habitat decline, difficult environmental conditions and predation. If P. gunnii are reintroduced to Woodlands again, then the models presented here may help to develop management strategies to establish a self sustaining population, as well as exploring translocations options.  相似文献   

Isoflavonoids isolated from Cuban propolis   总被引：3，自引：0，他引：3  

Piccinelli AL  Campo Fernandez M  Cuesta-Rubio O  Márquez Hernández I  De Simone F  Rastrelli L 《Journal of agricultural and food chemistry》2005,53(23):9010-9016

Chemical investigation of a red-type Cuban propolis sample has led to the isolation of 11 isoflavonoids (2 isoflavones, 3 isoflavans, and 6 pterocarpans), together with gallic acid, isoliquiritigenin, and (-)-liquiritigenin. Structural determination, including the absolute stereochemistry, was accomplished by spectroscopic analysis, particularly CD and 2D NMR techniques. The fragmentation behavior of pterocarpans was studied by electrospray ionization (ESI) tandem mass spectrometry (MS/MS) using an ion-trap analyzer, and a generalized fragmentation pathway, useful in the identification and structural characterization of pterocarpans, is proposed. Isoflavonoids are reported for the first time from propolis samples.  相似文献   

A new species of Mymarothecium and new host and geographical records for M. viatorum (Monogenea: Dactylogyridae), parasites of freshwater fishes in Brazil     

Cohen SC  Kohn A 《Folia parasitologica》2005,52(4):307-310

Mymarothecium boegeri sp. n. (Monogenea: Dactylogyridae) is described from the gills of Colossoma macropomum Cuvier (Characidae), collected from the aquaria of the "Centro de Pesquisas em Aquicultura, Departamento Nacional de Obras Contra as Secas (DNOCS)", Pentecoste City, State of Ceará, Brazil. Mymarothecium viatorum Boeger, Piasecki et Sobecka, 2002 is reported from the type host, Piaractus brachypomus (Cuvier) (Characidae) and from a new host, P. mesopotamicus (Holmberg) (Characidae), confirming the occurrence of M. viatorum in the Neotropical Region. The new species differs from the congeneric species in the structure of male copulatory complex; it is more closely related to M. viatorum by the presence of a posteromedial projection on ventral bar.  相似文献   

Zinc-responsive dermatosis in goats suggestive of hereditary malabsorption: two field cases     

Krametter-Froetscher R  Hauser S  Baumgartner W 《Veterinary dermatology》2005,16(4):269-275

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Comparison between carcass microbial load recovered by swabbing surfaces of different size and using the reference excision method     

Miraglia D  Ranucci D  D'Ovidio V  Branciari R  Severini M 《Veterinary research communications》2005,29(Z2):339-341

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Serum amyloid A isoforms in serum and synovial fluid in horses with lipopolysaccharide-induced arthritis     

Jacobsen S  Niewold TA  Halling-Thomsen M  Nanni S  Olsen E  Lindegaard C  Andersen PH 《Veterinary immunology and immunopathology》2006,110(3-4):325-330

The aim of the study was to determine the intraarticular serum amyloid A (SAA) response pattern in horses with inflammatory arthritis. Inflammatory arthritis was induced by injection of lipopolysaccharide (LPS) into the radiocarpal joint of four horses. Serum and synovial fluid (SF) samples were collected before and at 4, 8, 12, 24, 48, 72, 96, and 144 h after injection. Concentrations of SAA were measured by immunoturbidometry, and expression of SAA isoforms was visualized by denaturing isoelectric focusing and Western blotting. The LPS injection caused systemic and local clinical signs of inflammation. Serum amyloid A appeared in serum and SF within 8 h after LPS injection. Isoelectric focusing showed three major SAA bands with apparent isoelectric points (pI) of 7.9, 8.6, and >9.3 in serum and SF. Synovial fluid contained two additional isoforms with highly alkaline apparent pI values (apparent pI value extrapolated from standard curve = 10.0 and 10.2), which were not present in any of the serum samples. In conclusion, intraarticular injection of LPS induced systemic and local inflammatory responses in the horses. By demonstrating SF-specific SAA isoforms the results of the present study suggest that SAA is synthesized locally in the equine inflamed joint, similar to what has been demonstrated in humans previously. The marked local SAA synthesis suggests an important pathophysiological role in inflammatory arthritis.  相似文献   

Severe weight loss in lambs infected with Giardia duodenalis assemblage B     

Aloisio F  Filippini G  Antenucci P  Lepri E  Pezzotti G  Cacciò SM  Pozio E 《Veterinary parasitology》2006,142(1-2):154-158

An outbreak of giardiasis was observed in a sheep farm in Central Italy. Infected lambs (30-90 days of age) showed a malabsorption syndrome, decreased weight gain and impairment in feed efficiency. The most relevant clinical sign was the excretion of malodorous and poorly formed faeces, whereas diarrhoea was rarely observed in the flock. Laboratory investigations revealed the presence of Giardia in affected animals, while no other significant viral, bacterial or parasitic pathogens were identified in faeces or tissue samples. A mild to severe infiltrative enteritis with eosinophils, lymphocytes and plasma cells was detected in histological sections of the gut. Giardia parasites collected from duodenal aspirates were typed as Giardia duodenalis Assemblage B, by PCR amplification and sequencing of the TPI gene. Treatment with fenbendazole at a dose of 10mg/kg for 3 consecutive days, successfully cleared the infection. These results show that G. duodenalis can cause significant economic losses in sheep farming.  相似文献