Hemorrhagic diathesis and bone marrow aplasia secondary to lomustine overdose in a dog     

Luan C. Henker  Regina T. Kemper  Simone P. Bianchi  Marcele B. Bandinelli  Saulo P. Pavarini 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2019,48(2):255-258

A 9‐year‐old mixed breed 13 kg spayed female dog was presented for evaluation of two masses in the right abdominal mammary gland region. Surgery was conducted to excise the masses. A grade I complex mammary gland carcinoma and high grade (grade III) mast cell tumor with an inguinal lymph node metastasis were diagnosed. Forty‐seven days after the surgical procedure, the mast cell tumor relapsed, and neoadjuvant treatment with lomustine (81 mg/m²) was prescribed. Thirteen days from initiation of lomustine therapy, the dog was re‐presented to the hospital with bloody diarrhea, hematemesis, epistaxis, an elevated rectal temperature, depression, severe dehydration, and marked dyspnea. The CBC showed severe thrombocytopenia and leukopenia. According to the owner, lomustine (45mg per os [PO]) was mistakenly administered daily for 10 consecutive days (total dose, 810 mg/m²). The dog died and a necropsy was performed. The main gross lesions consisted of severe multifocal hemorrhages in multiple organs, especially in the digestive system. Histopathologic evaluation revealed disseminated hemorrhages, as well as marked bone marrow aplasia. This report describes the clinical, hematologic, gross, and histologic findings in a fatal case of lomustine overdose in a dog.  相似文献   

Use of reflectance measurements for the early detection of N,P, Mg,and Fe deficiencies in Zea mays L.     

Simone Graeff  Diedrich Steffens  Sven Schubert 《植物养料与土壤学杂志》2001,164(4):445-450

Mineral deficiencies can seriously reduce crop yield and economic returns to farmers. Reflectance measurements may provide inexpensive and fast estimates of the mineral status of plants. This study was conducted to examine specific changes of leaf reflectance due to nutrient deficiencies. During the 1998 and 1999 growing seasons leaf scans of N‐, P‐, Mg‐, and Fe‐deficient corn plants were performed with a digital LEICA S1 PRO camera under controlled light conditions. Leaf scans were evaluated with the L*a*b*‐color system. This is a three‐dimensional system with parameter a* describing the green/red percentage and parameter b* the blue/yellow percentage of a color. L* represents the lightness of a color. The a* and b* parameters provided good prediction of N, P, Mg, and Fe status of the plants in the wavelength ranges of 380—390 nm, 430—780 nm, 516—780 nm, 516—IR, and 540—600 nm because reflectance changed specifically due to the nutrient deficiency. Analyses of water‐soluble and propanol‐soluble pigments showed no significant changes in absorbance during latent deficiency. The results indicate that reflectance measurements may provide a powerful tool for the specific detection of latent nutrient deficiencies in corn plants.  相似文献   

Mortality outbreak by perch rhabdovirus in European perch (Perca fluviatilis) farmed in Italy: Clinical presentation and phylogenetic analysis     

Claudio Caruso  Andrea Gustinelli  Paolo Pastorino  Pier Luigi Acutis  Riccardo Prato  Loretta Masoero  Simone Peletto  Maria Letizia Fioravanti  Marino Prearo 《Journal of fish diseases》2019,42(5):773-776

This work reports a mortality outbreak, occurred in 2015 and affecting juveniles of European perch (Perca fluviatilis L.) farmed in Italy. Perch rhabdovirus (PRV) was detected by viral isolation and biomolecular investigations. Phylogenetic analysis clustered our isolate into genogroup B, which also includes PRV isolates from Perca fluviatilis identified in France (2004–2009); diagnostic investigations also revealed opportunistic bacteria (Aeromonas hydrophila) and parasites (Chilodonella piscicola). Since, occasionally, PRV has been reported in the natural environment, which is often a source of eggs and broodstock for farms, it could be possible that both similar France and Italian isolate were imported from a same place elsewhere and have a common origin. Improving biosecurity measures (batch control) and disinfection of egg strings with an iodine‐based solution helps prevent apparent vertical transmission of PRV.  相似文献   

Hydrological parameterization of piping in loess‐rich soils in the Bergisches Land,Nordrhein‐Westfalen,Germany     

Johannes Botschek  Simone Krause  Thekla Abel  Armin Skowronek 《植物养料与土壤学杂志》2002,165(4):506-510

Pedohydrological properties were investigated on a piped slope in the Bergisches Land in the Rhenish Slate Mountains (Nordrhein‐Westfalen, Germany). The study confirmed that genesis and development of pipes decisively depend on hydrological conditions in the soils. Vertical water permeability of saturated samples was very high. This promoted fast seepage. Many macropores produced by earthworms also caused high transport capacity for soil water. Even more efficient were the burrows of moles and mice, enabling immediate infiltration and direct vertical and lateral water movement. On the contrary, the horizontal saturated permeability was low indicating no correlation with piping. Porosity of piped soils was not different to that of other soils of the region without pipes.  相似文献   

Science mapping approach to analyze the research evolution on precision agriculture: world,EU and Italian situation     

Federico Pallottino  Marcello Biocca  Pierfrancesco Nardi  Simone Figorilli  Paolo Menesatti  Corrado Costa 《Precision Agriculture》2018,19(6):1011-1026

The relevance of precision agriculture produced a growth of the related literature over the years. However, a structured analysis of the published material is still missing. Thus, this study attempts to analyze the global scientific output of precision agriculture researches published during the period 2000–2016. By using a science mapping approach, mainly based on the application of network analysis tools, it was possible to investigate pivotal aspects of this research field such as publication trends, research topics and their geographical distribution. Using the Scopus database 17,756 scientific publications were retrieved from the chosen period. The number of publications increased after 2006, highlighting the vibrancy of the field. By authoring 35% of the publications, U.S.A. and China were the most active knowledge producer countries. Moreover, the generation of time resolved maps allowed us to identify agriculture engineering, computer science and agriculture studies as three main research areas characterizing precision agriculture panorama. The paper discusses the distribution of these topics at global level, among European countries and in Italy. Overall, this analysis represents the first holistic view of precision agriculture research providing valuable information for farmers, policy makers and researchers.  相似文献   

Detection of the F129L mutation in the cytochrome b gene in Phakopsora pachyrhizi          下载免费PDF全文

Ana C Klosowski  Louise L May De Mio  Simone Miessner  Ronaldo Rodrigues  Gerd Stammler 《Pest management science》2016,72(6):1211-1215

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Larvicidal activity of natural and modified triterpenoids against Aedes aegypti (Diptera: Culicidae)          下载免费PDF全文

Gloria NS da Silva  Frances TT Trindade  Francine dos Santos  Grace Gosmann  Alexandre A e Silva  Simone CB Gnoatto 《Pest management science》2016,72(10):1883-1887

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Cadmium-Containing Carbonic Anhydrase CDCA1 in Marine Diatom Thalassiosira weissflogii     

Vincenzo Alterio  Emma Langella  Giuseppina De Simone  Simona Maria Monti 《Marine drugs》2015,13(4):1688-1697

The Carbon Concentration Mechanism (CCM) allows phytoplakton species to accumulate the dissolved inorganic carbon (DIC) necessary for an efficient photosynthesis even under carbon dioxide limitation. In this mechanism of primary importance for diatoms, a key role is played by carbonic anhydrase (CA) enzymes which catalyze the reversible hydration of CO₂, thus taking part in the acquisition of inorganic carbon for photosynthesis. A novel CA, named CDCA1, has been recently discovered in the marine diatom Thalassiosira weissflogii. CDCA1 is a cambialistic enzyme since it naturally uses Cd²⁺ as catalytic metal ion, but if necessary can spontaneously exchange Cd²⁺ to Zn²⁺. Here, the biochemical and structural features of CDCA1 enzyme will be presented together with its putative biotechnological applications for the detection of metal ions in seawaters.  相似文献   

ROS and 9-oxylipins are correlated with deoxynivalenol accumulation in the germinating caryopses of Triticum aestivum after Fusarium graminearum infection     

Chiara Nobili  Simone D’Angeli  Maria Maddalena Altamura  Valeria Scala  Anna Adele Fabbri  Massimo Reverberi  Corrado Fanelli 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(2):423-438

Wheat germinating caryopses may represent a starting point for the Fusarium Head Blight disease; however, only few studies concern the defence repertoire of wheat caryopses against fungal challenge. The germinating caryopses of two wheat commercial varieties (Blasco and Sagittario), differentially susceptible to FHB in the field, were inoculated with F. graminearum and the redox status in the interaction milieu, oxylipin production, the expression profile of some host-defence related genes, and programmed cell death in the aleuronic layer, were analysed. In Sagittario, the redox balance was profoundly modified and 9-oxylipins accumulated during fungal contamination. In this variety, F. graminearum produced a high quantity of deoxynivalenol whilst programmed cell death, also through metacaspases activation, was enhanced in the aleuronic layer of its caryopses. In Blasco, the expression of tolerance factors such as Pathogenesis-Related-protein1, glucosyl-transferase and glutathione transferase genes was up-regulated consequent to infection. Results show that unscavenged ROS and 9-oxylipins may be related to deoxynivalenol accumulation in the germinating caryopses of wheat after F. graminearum infection.  相似文献   

Chlorogenic acid,a metabolite identified by untargeted metabolome analysis in resistant tomatoes,inhibits the colonization by Alternaria alternata by inhibiting alternariol biosynthesis     

Edyta Wojciechowska  Christoph H. Weinert  Björn Egert  Bernhard Trierweiler  Markus Schmidt-Heydt  Bernd Horneburg  Simone Graeff-Hönninger  Sabine E. Kulling  Rolf Geisen 《European journal of plant pathology / European Foundation for Plant Pathology》2014,139(4):735-747

Tomato fruits can be contaminated by saprophytic strains of Alternaria alternata which is the reason for the frequent occurrence of Alternaria toxins like alternariol, alternariol monomethylether or tenuazonic acid in these types of products. It was shown earlier that alternariol is a colonization factor for tomatoes. In the current analysis two different tomato genotypes were analysed by untargeted comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-MS). This analysis revealed clear differences in the metabolic profiles which were paralleled by differences in resistance towards Alternaria colonization. One of the genotypes was more resistant against A. alternata infection and contained high amounts of chlorogenic acid in contrast to the other genotype which was sensitive against infection. In in vitro analysis, chlorogenic acid reduced alternariol biosynthesis during the first days of growth of A. alternata. Expression analysis of the alternariol polyketide synthase gene, a key gene in the biosynthesis of alternariol, also revealed a temporal reduction in its expression in the first phases of growth. However by chromatographic analysis it could be demonstrated that chlorogenic acid was degraded over time. This degradation leads to a relief of inhibition resulting in an only temporal inhibition of alternariol biosynthesis. In vivo colonization experiments revealed that chlorogenic acid reduces colonization of tomatoes by A. alternata in a concentration dependent manner, which however is partly counteracted by the addition of alterariol.  相似文献