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Purpose
It is critical to understand the effect of manure application on the availability of phosphorus (P) and the potential environmental contamination by runoff and leaching. However, previous studies generally focused on cultivated soil layer in single cropping systems. The aim of this study was to evaluate the effect of manure application on soil P forms and quantities to the 200 cm depth in a Chinese alkaline Cambisol in different cropping systems and the potential environmental implications.Materials and methods
The sampling site, Shunyi District, is located in the peri-urban area of Beijing in the North China Plain, where large quantities of manure generated from intensive animal operations have been applied to agricultural fields. A field survey was carried out before sampling to identify soil sampling sites with long-term manure application and an adjacent area receiving no manure used for the same crop production. Soil samples from three cropping systems (vegetables, cereals, and trees) were vertically collected to a depth of 200 cm with the following depth increments: 0–20, 20–60, 60–90, 90–120, 120–160 and 160–200 cm. Soil samples were analyzed for plant-available P (Olsen P) and various P fractions by sequential P fractionation. Degree of P saturation (DPS) was also determined.Results and discussion
Soil calcium bound P was the most abundant P fraction, followed by the residual P. Organic P only accounted for less than 5 % of total P in most of the soils. Manure application increased the levels of inorganic P (Pi), with higher proportions of Pi in labile forms than stable forms. After manure application for 8–15 year, available P (Olsen P) and DPS values of the 0–20 cm layer in all sites exceeded the threshold for Olsen P (60 mg?kg?1) and DPS (30 %) and the risk of P loss by runoff is expected to significantly increase. The DPS values were generally lower than 30 % below 20 cm, indicating a minimal risk of P loss via leaching from deeper soil.Conclusions
The results indicated that in typical peri-urban areas of the North China Plain, the on-going practice of manure application not only increased the size of each of the labile and non-labile P pools, but also caused a shift in the relative sizes of the different pools, regardless of the cropping systems. However, contrary to what was expected, soil P loss through surface runoff would be a greater concern than leaching following long-term manure amendment. 相似文献Salinity is a crucial problem which has affected crop productivity globally. Ascorbic acid is considered helpful against abiotic stresses due to its powerful antioxidant potential. In the pot experiment, salinity stress (0, 35, 70, and 105?mM) was applied to sweet peppers in split doses after 20 days of transplantation. To mitigate the adverse effects of salinity, ascorbic acid (0, 0.40, 0.80, and 1.20?mM) was applied as foliar spray after a 6-day interval during vegetative growth. Sweet pepper plants sprayed with distilled water (control) recorded maximum plant height (cm), leaf area (cm2), number of branches, stem diameter (mm), number of fruit plant?1, fruit diameter (cm), yield plant?1 (g), and chlorophyll content (mg 100?g?1), while the maximum polyphenol oxidase (PPO) activity (unit mg protein?1 min?1) and ascorbate peroxidase (APX) activity (unit mg protein?1 min?1) were recorded in plants treated with 70?mM NaCl application. Salinity stress beyond 70?mM significantly reduced all the studied parameters. An ascorbic acid concentration of 1.20?mM significantly mitigated the negative effects of salt stress and recorded maximum plant height (cm), number of leaves plant?1, leaf area (cm2), number of branches plant?1, stem diameter (mm), number of fruit plant?1, fruit diameter (cm), yield plant?1 (g), chlorophyll content (mg 100?g?1), PPO activity (unit mg protein?1 min?1), and APX activity (unit mg protein?1 min?1). Hence, a 1.20?mM concentration of foliar ascorbic acid could be used in saline conditions up to 70?mM of sodium chloride (NaCl) for better growth, productivity, and enzymatic activity of sweet peppers.
相似文献Wheat (Triticum aestivum L.) is a staple food in many countries and is regarded as a vital source of nutrition. Drought is one the most prevalent limitations to wheat growth and development. Herein a two year study was conducted using 25 diverse wheat genotypes obtained from the gene pool of various research institutes of Pakistan to characterize their drought tolerance using various physiological indices like relative water content (RWC), relative dry weight (RDW), water saturation deficit (WSD), relative water loss (RWL), flag leaf area (LA), chlorophyll content index (CC) and their association with the grain yield (GY). Analysis of variance (ANOVA) indicated the presence of significant amount of differences and genetic diversity among genotypes under study. Correlation analysis exposed positive association of CC and LA with GY. However, RWC was shown to have a highly significant and negative association with WSD and RWL. Principal component analysis (PCA) showed that out of the 7 PCs only 2 were significant having eigenvalues >?1; cumulatively accounting for 88.70% and 73.03% of the total variation under control and drought stress conditions, respectively. Strikingly the results of the PCA biplots and cluster heat map exposed G1 (Barani-17), G2 (Dharabi-11), G3 (Ehsan-16), G4 (Chakwal-50), G17 (Ujala-2016) and G23 (Kohistan-97) as potential drought tolerant genotypes. Selection of the positively associated indices would be fruitful and the tolerant genotypes having drought tolerance potential could be utilized in future wheat breeding programs to develop high yielding and drought tolerant genotypes.
相似文献Seed viability monitoring is very important in ex situ germplasm preservation to detect germplasm deterioration. This requires seed-, time- and labor- saving methods with high precision to assess seed germination as viability. Although the current non-invasive, rapid, sensing methods (NRSs) are time- and labor-saving, they lack the precision and simplicity which are the virtues of traditional germination. Moreover, they consume a considerable amount of seeds to adjust sensed signals to germination percentage, which disregards the seed-saving objective. This becomes particularly severe for rare or endangered species whose seeds are already scarce. Here we propose a new method that is precise, low-invasive, simple, and quick, which involves analyzing the pattern of dehiscence (seed coat rupture), followed by embryonic protrusion.
ResultsDehiscence proved simple to identify. After the trial of 20 treatments from 3 rice varieties, we recognized that dehiscence percentage at the 48th hour of germination (D(48)) correlates significantly with germination rate for tested seed lots. In addition, we found that the final germination percentage corresponded to D(48) plus 5. More than 70% of the seeds survived post-dehiscence desiccation for storage. Hydrogen peroxide (1 mM) as the solution for imbibition could further improve the survival. The method also worked quicker than tetrazolium which is honored as a fast, traditional method, in detecting less vigorous but viable seeds.
ConclusionWe demonstrated the comprehensive virtues of dehiscence method in assessing rice seed: it is more precise and easier to use than NRSs and is faster and more seed-saving than traditional methods. We anticipate modifications including artificial intelligence to extend our method to increasingly diverse circumstances and species.
相似文献Fungal toxins in feed are leading issue in poultry industry causing a detrimental effect on the performance and health of poultry. The study was carried out to determine the incidence and concentration of the aflatoxins and their major producer Aspergillus flavus in home mix layer poultry feed in respect of seasonal variation throughout the year. A total of (n?= 204) home mix poultry layer feed samples were analyzed for the isolation of fungi. The isolates were initially screened through colony morphology and microscopic examination. However, aflatoxin concentration was determined by ELISA. Revealed results indicated that, the highest percentage of A. flavus was found during the months of June to August 50/54 (92.5%) followed by September to November 43/65 (66.1%), March to May 21/40 (52.5%), and December to February 18/45 (40%). As a whole, the incidence was recorded 132/204 (64.7%). Moreover, of the 132 samples, 41 (31%) were exceeded in respect of aflatoxin contamination from the legal limit (20 μg/kg) imposed by Food Drug Association (FDA). Statistically, the growth of A. flavus and aflatoxin production was found significantly different in respect of seasonal variation. As highest total viable fungal count (9.9?×?104 CFU/g) and aflatoxin level (72.27 μg/kg) were recorded during the months of June to August and lowest in December to February. Consequently, instantaneous essential control measures are demanded regarding appropriate storage and adequate drying in post-harvesting season. Along with surveillance plans and austere regulations for monitoring the aflatoxin contents for the wellbeing of consumers.
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