Role of calcineurin in airway remodeling in guinea pig model of asthma     

CHEN Ya-hong  ZHAO Ming-wu  FU Min-gui  YAO Wan-zhen  WANG Xiao-hong  WANG Jian-li  TANG Chao-shu 《园艺学报》2002,18(2):157-160

AIM:To investigate the role of calcineurin (CaN) in airway remodeling in guinea pig model of asthma.METHODS:Male guinea pigs were randomly divided into three groups: control, asthma group and CsA group. The following parameters were measured: 1. The protein content, cell count and differential count of BALF; 2. The amount of [³H]-TdR incorporation into central airway smooth muscle; 3. The mean thickness of airway wall and airway smooth muscle of small airwaysl; 4.CaN activity of trachea and lung tissue.RESULTS:1. The protein content, cell count and eosinophil of BALF in CsA group were 46%, 51% and 60% lower than those in asthma group, respectively (P＜0.01); 2. [³H]-TdR incorporation in CsA group was 22% lower than that in asthma group (P＜0.05);3. The mean thickness of airway wall and airway smooth muscle were 34% and 37% less in CsA group than those in asthma group, respectively (P＜0.01); 4. CaN activity of lung tissue and trachea were 52% and 44% lower in CsA group than those in asthma group, respectively (P＜0.01).CONCLUSION:CsA reduced airway remodeling in guinea pig model of asthma, indicating the role of CaN in the airway remodeling.  相似文献   

Preparation of gfp-bcl-X_L-contained recombinant adenovirus vector by the homologous recombination in bacteria     

LUO Yan  TANG Shi-bo  LI Xiang-hong  LIU Shuang-xin 《园艺学报》2002,18(12):1462-1467

AIM: To prepare gfp-bcl-X_L-contained recombinant adenovirus(rAd-gfp-bcl-X_L).METHODS: Bcl-X_L gene was amplified from pEGFP-C₃-bcl-X_L, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-X_L. Then pAdTrack-CMV-bcl-X_L was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-X_L and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-X_L into 293 cells. PCR test indicated that the recombinant Ad contained bcl-X_L gene. The titer of the purified rAd-gfp-bcl-X_L was 6.5×10¹² PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-X_L. This affords a good gene transfer vector for the gene therapy in human’s diseases.  相似文献   

Effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts     

REN Yu-sheng  WU Zong-gui  CUI Fang  JIA Guo-liang  YU Shi-qing  TANG Chao-wu  LI Bo 《园艺学报》2002,18(11):1377-1380

AIM: To investigate the effects of platelet-derived growth factor on DNA and collagen protein synthesis in human vascular fibroblasts. METHODS: In the present experiment, the human vascular fibroblasts were cultured and effects of platelet-derived growth factor-BB on DNA and collagen protein synthesis in human vascular fibroblasts were observed by using [³H]-TdR incorporation and [³H]-proline incorporation in vitro. RESULTS: Platelet-derived growth factor-BB significantly promoted NDA synthesis and collagen protein synthesis of quiescent human vascular fibroblasts, with a maximal response at a concentration of 30μg·L^-1at 24 h and 36 h, respectively. CONCLUSION: Platelet-derived growth factor-BB promotes DNA and collagen protein synthesis in cultured human vascular fibroblasts.  相似文献   

Effects of carvedilol,cilazapril and their combination on left ventricular remodeling after acute myocardial infarction in rats     

TANG Yi-da  YANG Yue-jin  RUAN Ying-mao  LI Yong-li  ZHOU Yan-wen  TIAN Yi  GAO Run-lin  CHEN Ji-lin  CHEN Zai-jia 《园艺学报》2002,18(9):1115-1118

AIM:To compare the effects of carvedilol, cilazapril and their combination on left ventricular remodeling(LVRM) after acute myocardial infarction(AMI) in rats. METHODS: Twenty-four hours after AMI operation, 100 surviving rats were randomly assigned to: ①AMI control(n= 25), ②AMI+carvedilol(1 mg·kg^-1 ·d^-1, n= 25)(C1), ③AMI+cilazapril(1 mg·kg^-1 ·d^-1, n= 25)(Z1), and ④ AMI+combination(n= 25) groups. Sham-operated group(n= 17) were selected randomly. After 4 weeks of therapy with the drugs gastric gavage, hemodynamic and pathological studies were performed. RESULTS: There were no significant differences in MI size among the four AMI groups(all P> 0.05) Left ventricular(LV) end diastolic pressure(LVEDP), volume(LVV), weight(LVW) and septal thickness(STh) were all higher and left ventricular pressure maximal rate of rise and fall(±d p /d t) were lower(all P< 0.01) in AMI group than sham-operated group. The LVEDP, LVV, LVW and STh were all lower and ±dp /dt were higher in Z1, C1, and combination groups than those in AMI group(P< 0.05, P< 0.01), with LVEDP and STh were more lower in the combination group than in the two monotherapy group(P< 0.05, P< 0.01), but there were no significant differences in other variables among the three therapy groups. CONCLUSION: Carvedilol, cilazapril and their combination all can prevent from LVRM after AMI in rats, improve hemodynamics and LV function, with the combination superior.  相似文献   

Protein expression of cyclin D₂ and p16 in proliferation and differentiation of cultured cardiac myocytes     

ZHANG Yu-xia  YU Lun-yin  LIU Ming-qiu  ZHANG Zheng-bin TANG Zhi-jiao  XIA Dong  WANG Ming 《园艺学报》2002,18(1):32-35

AIM:To investigate the protein expression of cyclin D₂ and p16 in proliferation and differentiation of cultured cardiac myocytes.METHODS:One-day-old Sparague-Dawley rats were used. Cardiac myocytes(CM) were collected by a trypsin-dispersal method and cultured. Cell growth line and fluorescence activated cell sorting (FACS) were used to investigate the proliferation of CM. Ultra-thin sections were made to observe the ultrastructure of CM under transmission electron microscope. The expression of cyclin D₂ and p16 in CM were measured using immunocytochemistry and image analysis.RESULTS:①Results of cell growth line and FACS analysis showed that cultured CM could proliferate in the first 3 cultured days, but the ability decreased quickly, concomitant with differentiation. CM was obseved quiescent in cell cycle three days later. The ultrastructure of CM showed the large amount of myofilaments and mitochondrion. ②The protein expression of cyclin D₂ in 3,4,5 day CM group was 0.89 times(P＜0.05),0.80 times (P＜0.05) and 0.56 times (P＜0.01) of that in 1 day group, respectively. The expression of p16 in CM was increased during the culture process, 2,3,4,5 day group were 1.63 times, 1.72 times, 1.99 times and 2.84 times (P＜0.01) of that in 1 day group, respectively.CONCLUSION:Cultured neonatal rat cardiac myocytes could proliferate during the first 3 days after incubation, but the ability of proliferation decreased, from the fourth day, concomitant with differentiation. Cyclin D₂ and p16 play the key roles in CM postnatal development. Downregulation of cyclin D₂ and upregulation of p16 may induce CM differentiation.  相似文献   

昆虫神经肽类激素的研究及应用前景          下载免费PDF全文

欧阳迎春  唐爽  关雪辰 《植物保护》2003,29(1):9-11

由昆虫脑神经内分泌细胞分泌的神经肽类激素 ,咽侧体活化激素 (allatotropin ,AT)和咽侧体抑制激素 (al latostatin ,AS) ,分别刺激或抑制咽侧体的保幼激素的生物合成 ,从而控制昆虫的生长、发育和变态。利用昆虫神经内分泌的失调 ,使其体内激素水平失调 ,来达到控制害虫的目的。  相似文献   

根结线虫的研究现状   总被引：50，自引：0，他引：50  

赵鸿  彭德良  朱建兰 《植物保护》2003,29(6):6-9

概述了根结线虫的发生分布和传统分类鉴定,以及分子生物学技术(同工酶电泳技术、DNA重组技术、PCR技术)在根结线虫种和生理小种鉴定中的运用及其防治,并对生物防治及抗根结线虫育种、转基因工程在植物线虫学研究领域上的应用前景作了展望。  相似文献   

食用菌复合保水颗粒菌种生产工艺及其应用研究   总被引：3，自引：0，他引：3  

冀宏  陈文杰  霍红  韩韬  朱朝辉 《食用菌学报》2003,10(1):42-45

运用微生物发酵技术，配合新型合成材料，研制出食用菌复合保水颗粒菌种。这类菌种可保持活力60d。这一工艺可用于糙皮侧耳、金针菇、灵芝、白灵菇、杏鲍菇、鸡腿蘑和鲍鱼菇等多种食用菌苗种的制作。栽培试验证明，此类菌种的菌丝生长速度、现蕾时间、生物学效率与常用的棉籽壳菌种相似。  相似文献   

阿魏蘑与杏鲍菇高产优质栽培模式研究   总被引：2，自引：0，他引：2  

万鲁长  张柏松  黄春燕  郭惠东  单洪涛  李斌  姜春君  陈黎明  姚建国 《食用菌学报》2003,10(2):40-44

试验证明，阿魏蘑和杏鲍菇最佳栽培模式为菌棒半脱袋竖向畦栽半覆土栽培模式；最适环境条件为菇房温度13～20℃，空气相对湿度85％～95％；最佳栽培设施为半地下冬暖式塑料大棚。  相似文献   

金耳的液体发酵研究     

汪虹  瞿伟菁  曹群华 《食用菌学报》2003,10(4):29-33

研究表明，金耳(Tremella aurantialba)液体发酵最适培养基组成为玉米粉2％，蔗糖3％，酵母膏0．1％，KH2PO40．1％，MgSO40．05％；培养基最佳初始pH、250mL三角瓶装量、接种量、种子菌龄分别是3．5、80mL、10％和120h；用10L发酵罐发酵时，最佳培养条件是种子种龄144h、28℃、接种量10％、通风量2．5L／min。  相似文献