北川白山羊基因组与表型性状相关的多态性RAPD标记     

蔡欣  苟兴能  董绍森  刘畅  王雪  李勇 《中国兽医学报》2011,31(10)

北川白山羊是在特定生态条件下未经系统繁育而形成的优良地方山羊品种,在生长发育、繁殖和抗逆等性状方面具有突出优势。通过对122只北川白山羊个体基因组的扩增,结果从80条RAPD引物中选出12条多态引物,北川白山羊群体平均遗传相似率和遗传多样性指数分别为0.859 8±0.077 6和0.919 8,表明北川白山羊品种具有一定的遗传分化和较丰富的遗传多样性。在12条多态性引物中,SBS06同时对体质量（P=0.028）、体高（P=0.017）和体长（P=0.037）具有显著影响,SBS02对体质量（P=0.033）和体高（P=0.034）具有显著效应,所以推断影响体质量和体高性状的QTL基因座可能与RAPD标记SBS06和SBS02相连锁,可以应用于北川白山羊体质量和体高性状的标记辅助选择以及相应主效基因的进一步研究。  相似文献   

农用无人机移动补给平台自主降落算法与试验     

祖林禄  侯加林  陈民  王红  苏斐 《农业机械学报》2020,51(3):43-50

为实现根据无人机作业位置改变中途补充能源或喷洒物的起降地点,增加无人机有效作业时间,提高无人机作业效率,设计了无人机移动补给平台。通过研究农用无人机自主降落过程,提出一种基于模糊逻辑和比例积分微分(Proportional-integral and derivative,PID)分段控制的农用无人机跟踪降落算法,该算法既拥有PID算法的高精度,又兼顾模糊控制算法响应速度快、超调量小、鲁棒性强的优点。目标轨迹跟踪预测由粒子滤波器跟踪算法和轨迹拟合算法相结合进行求解。仿真和现场试验表明,与单一的PID算法和模糊逻辑算法相比,分段控制算法能够把农用无人机对移动补给平台的跟踪误差缩小到6. 7 cm以内,在移动补给平台上的降落精度控制在7. 2 cm以内。  相似文献   

我国草地贪夜蛾的防控对策与建议   总被引：11，自引：0，他引：11  

杨普云  朱晓明  郭井菲  王振营 《植物保护》2019,45(4):1-6

草地贪夜蛾Spodoptera frugiperda(J. E. Smith)是世界性重大农业害虫,该虫自2019年1月入侵我国云南省到5月下旬,在不到5个月的时间里就已经入侵了长江流域及其以南的15个省(市、自治区)。预计6月和7月将到达我国的玉米主产区黄淮海夏玉米区和北方春玉米区,受威胁玉米面积1 300多万hm~2,将严重威胁我国的玉米生产安全。我国玉米的种植布局随季节和纬度变化从南至北递次推移,时间和空间上互补,为草地贪夜蛾提供了丰富的食物资源,为其种群区域性迁移和周年繁殖为害提供了良好的自然条件。为做好草地贪夜蛾的防控工作,及时控制其为害,本文在总结国内外防控草地贪夜蛾经验教训的基础上,初步提出了我国草地贪夜蛾的防控对策。  相似文献   

小菊新品种‘枫林黄星’和‘枫林红晕’     

张蒙蒙  戴思兰  王青  方志军  季玉山  王朔 《园艺学报》2019,46(1):197-198

小菊新品种‘枫林黄星’是以‘Branice Sunny’为母本,‘183’株系为父本经人工杂交选育而成,舌状花亮黄色,头状花序直径3.7 cm,冠幅63.8 cm,单株着花1 007朵。‘枫林红晕’是以‘388’株系为母本,从德国引进的多个品种为父本混合授粉选育而成,舌状花中上部粉色,下部白色,头状花序直径3.6 cm,冠幅60.2 cm,单株着花847朵。两个新品种冠幅大,株形紧凑丰满,呈半圆形,群体效果良好,适于北京地区秋季绿化。  相似文献   

Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes     

WANG Huaqing  GAO Qingshan  JIANG Wenjie  ZHAO Yuhan  JIN Qingguo  LI Yinghua  XU Yongnan 《中国畜牧兽医》2019,46(2):497-503

To investigate the effect of histone deacetylation inhibitor Psammaplin A (PsA) on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group (PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species (ROS),glutathione (GSH) and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups (P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group (P>0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group (P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group (P>0.05).The apoptosis rate in aging group was significantly higher than that of control and PsA groups (P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group (P<0.05).The GSH level of MⅡ oocytes in aging group was significantly lower than that of control and PsA groups (P<0.05).There was no significant difference in GSH level between control and PsA groups (P>0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups (P<0.05).The ROS level in PsA group was significantly higher than that of control group (P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups (P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group (P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.  相似文献   

无人机施药对棉蚜的防治效果及经济效益分析   总被引：1，自引：0，他引：1  

王喆  冯宏祖  马小艳  王兰  武刚  苟长青 《农药学学报》2019,21(3):366-371

为探讨采用无人机防治新疆南部地区棉蚜的可行性并筛选防治效果好的杀虫剂及具有增效作用的杀虫剂助剂，研究了采用大疆MG-1S型无人机喷施不同的杀虫剂+助剂对棉蚜的防治效果，并对无人机飞防的经济效益进行了分析。结果表明:采用MG-1S型无人机喷施22%氟啶虫胺腈悬浮剂(SC) 225 mL/hm2(制剂量，下同)时对棉蚜的防治效果最好，优于喷施70%吡虫啉水分散粒剂(WG) 30 g/hm2和70%啶虫咪WG 60 g/hm2。在添加助剂方面，以分别在70%啶虫脒WG 60g/hm2和70%吡虫啉WG 30g/hm2中添加15 mL/hm2的高工效通用助剂的防治效果最好，其次为聚合物类助剂和飞防增效剂；而在22%氟啶虫胺腈SC(150 mL/hm2)中，以添加150 mL/hm2的飞防增效剂的效果最好，其次为矿物源类、有机硅类和聚合物类助剂。采用无人机施药，在施药后3 d和5 d的防治效果与采用机械+人工拖管施药相比均无显著性差异，但无人机田间作业施药液量减少1/3，工作效率提高3倍，机械作业费减少60元/hm2，可满足现代农业高效、节药和降低成本的需要。  相似文献   

核桃枝枯病病原菌鉴定及其生物学特性     

刘静  孙翠翠  薛德胜  李保华  练森  王彩霞 《植物病理学报》2020,50(6):774-778

In May 2018,a new shoot dieback disease was found in several walnut orchards in Rizhao,Shandong Province.The fungal pathogen was obtained on PDA plate from diseased walnut shoots using a tissue isolation method.The purified fungal isolates formed white to grey colonies on PDA plate and three single spore isolates SDWa1-SDWa3 were obtained.Based on morphological characteristics and analysis of rDNA internal transcribed spacer (ITS),calmodulin (CAL),translation elongation factor 1-α (TEF1) and β-tubulin (TUB) sequences,the fungal isolates were identified as Diaporthe nobilis,a new pathogen on walnut.The test of Koch's rule confirmed that D.nobilis was the pathogen of walnut shoot dieback.To determine optimal growing conditions for the pathogen,its biological characteristics test was conducted.The results showed that the optimal growth temperature and pH value were at 25℃ and pH 5-9,respectively.The optimum carbon source was glucose,and favourable nitrogen source was peptone.  相似文献   

优质弱筋小麦品种黔麦21号的选育与应用     

王伟  隋建枢  陈天青  罗永露  吴文强  何庆才 《耕作与栽培》2020,40(4):46-47

  相似文献   

河北省水稻生产概况及产业发展展望     

王亚楠  孙立永  贾文冬  许春  张启星 《河北农业科学》2020,24(2):13-14,62

河北省地处我国水稻生育期最长地带,具有得天独厚生产优质米的条件,通过优质品种培育、新技术研发推广和品牌创建,助推河北省水稻由生产小省向效益强省迈进。概述了河北省水稻主产区的生产现状,分析了水稻产业发展中存在的问题,并提出了未来产业发展的主攻方向。  相似文献   

不同春小麦品种耐低磷性评价及种质筛选   总被引：2，自引：0，他引：2  

刘露露  汪军成  姚立蓉  孟亚雄  李葆春  杨轲  司二静  王化俊  马小乐  尚勋武  李兴茂 《中国生态农业学报》2020,28(7):999-1009

筛选磷高效作物是充分利用土壤磷素和减少磷肥施用量的重要手段。本研究以162份春小麦种质资源为材料,对其苗期的株高、总根长、根表面积等8个指标的耐低磷系数进行分析,采用隶属函数法综合评价春小麦苗期的耐低磷特性,初步筛选耐低磷材料,并进一步进行成株期的耐低磷特性鉴定,筛选出耐低磷材料和磷敏感材料,分析其在低磷下酸性磷酸酶的活性变化。结果表明,低磷胁迫下春小麦材料苗期和成株期的各性状均受到不同程度的影响,并随着胁迫程度的增加,小麦生长受抑制程度增强。通过主成分分析将苗期8个指标转化成4个综合指标(累计贡献率为82.60%),将成株期的10个指标转化为3个综合指标(累计贡献率为83.23%);采用隶属函数法计算耐低磷综合评价值(D)值,对D值进行聚类分析,将苗期的162份春小麦种质资源划分为耐低磷型(10份)、较耐低磷型(26份)、低磷较敏感型(91份)、低磷敏感型(35份)4类。选取耐低磷型材料(5份)和低磷敏感型材料(4份),进一步进行成株期鉴定,最终筛选1份耐低磷材料wp-35和1份磷敏感材料wp-119。通过分析其酸性磷酸酶活性,发现在低磷胁迫下春小麦根系和叶片中的酸性磷酸酶活性均升高,且耐低磷材料的酸性磷酸酶活性高于磷敏感材料。本研究结果可为解析春小麦耐低磷特性、培育耐低磷品种提供种质资源和理论依据。  相似文献