几种药剂防治板栗栗绛蚧药效试验     

吴浙东 《落叶果树》2000,32(4):51-51

栗绛蚧近年来已成为浙中地区板栗的主要害虫之一。通过药剂筛选,表明快克、毒死蜱是目前防治栗绛蚧的理想药剂,喷药后８天校正防效分别为９８．３％和９５．８％。  相似文献   

Effects of glucose and Mg²⁺ in the neurons damaged by glutamate     

XING Hong  HE Qi-hua  YUAN Lan  XU Jia-ling  WU Ben-jie 《园艺学报》2000,16(6):508-511

AIM and METHODS: To observe the effects of glucose-free and Mg²⁺-free in the extracellular fluid on the changes of [Ca ²⁺]_i in the cerebro-cortical neurons damaged by 1mmol/L glutamate using laser confocal scanning microscope. RESULTS: Both frequency and amplitude of neuronal calcium oscillation induced by glutamate were lowered in glucose-free and Mg²⁺-free buffers. The basic [Ca²⁺]_i concentration was lowered in the former case , but it was elevated in the latter case. CONCLUSION: Mg²⁺-free aggravates [Ca²⁺]_i overload induced by 1mmol/L glutamate ,under certain conditions the glucose-free might resist damage role of glutamate and Mg²⁺-free.  相似文献   

The protection of the hepatic ischemic preconditioning is concerned with the NO/ET-1 system     

LU Ping  CHEN Dao-da  TIAN Yuan  ZHANG Jing-hui  WU Yi-hua 《园艺学报》2000,16(10):901-905

AIM: To study the relationship between the disturbance of nitric oxide/endothelin-1(NO/ET-1) and hepatic ischemia/reperfusion(I/R) injury as well as the regulation of NO/ET-1 system by hepatic ischemic preconditioning(IPC). METHODS: The changes of NO/ET-1 system and their relationship with hepatic I/R injury were compared between I/R group and IPC+I/R group in a rat hepatic I/R model. Two hours after reperfusion, the liver tissues were detected by RT-PCR to see whether there was inducible nitric oxide synthase (iNOS) mRNA expression. RESULTS:In the acute phase of hepatic reperfusion, the ratio of NO/ET-1 was reduced, which was due to a significant reduction of NO₂^-/NO₃^- (the metabolic product of NO) and significant elevation of ET-1 in the blood plasma. The content of ALT, AST, LDH and TNF-α in blood plasma, and of MDA in liver tissue were increased but ATP in liver tissue was reduced, the hepatic damage was deteriorated. The protection of the hepatic IPC was concerned with the elevation of the ratio of NO/ET-1 caused by the elevation of NO₂^-/NO₃^-, and reduction of ET-1 as well. There was no iNOS mRNA detected in the liver tissues.CONCLUSION: Hepatic I/R injury is related to the disturbance of NO/ET-1. The protection of the hepatic IPC in the acute phase might be conducted by its regulation of NO/ET-1 system. The cNOS rather than the iNOS generated the NO in this situation.  相似文献   

Mitochondria respiratory chain related genes expression in post ischemia/reperfusion rat     

WU Qi  DENG Xiao-ming  YUAN You-zhong  XIONG Yuan-chang  LIU Shu-xiao 《园艺学报》2000,16(8):722-725

  相似文献   

Correlation between intraventricular pressure and early phase proto-oncogene expression in volume overload rats     

HU Xiao-ping  LI He  FEI Jian  ZHANG Guo-yuan  WU Zong-gui 《园艺学报》2000,16(7):592-595

AIM: To investigate the effect of intraventricular pressure change by volume overload (VOL) on expression of proto-oncogene c-fos,c-jun,c-myc and egr-1. METHODS: Left ventricular systolic pressure(LVSP) and left ventricular end diastolic pressure (LVEDP) of rat with VOL induced by aortacaval fistula operation and rats of control group were measured at 30 min, 1, 4, 6, 12 and 48 h after the operation,these mRNAs at the foregoing time points were measured by slot bloting method and quantified with densitometry. RESULTS: Be compared with the control group, VOL rats LVSP decreased (P＜0.05) and declined most remarkably at 48 h,LVEDP elevated significantly at 30 min (P＜0.05), reached a maximal value at 12 h and the levels of control group at 48 h (P＞0.05) after the operation.The proto-oncogene expression signals were not detected in the control,negative controls and VOL rats at 30 min after the operation. The c-fos,c-jun and egr-1 mRNA signals appeared earlier,at 1 h, and c-myc mRNA increased later at 4 h.All reached peak value at 4 h and then declined gradually.The c-fos mRNA were not detected at 48 h. The c-myc,c-0jun and egr-1 mRNA persisted throught the entire observation period from 1 h to 48 h. CONCLUSIONS: During VOL early phase the overload have effect on expression of the proto-oncogene mRNA,c-fos,c-jun and egr-1 mRNA appear earlier, c-myc later,egr-1,c-jun and c-myc persist longer period, but the expressions do not strengthen with the ventricule wall load increase.This sequential induction pattern may reflect the time course regularity of the proto-oncogenes expression induced by VOL,and indicate the proto-oncogenes expression initiate while the heart load accumulate some extent and duration and the load magnitude may not play a critical role.  相似文献   

Effects of berberine on IL-1 or tumour necrosis factor induced polymorphonuclear leucocyte－endothelium adhesion     

HAO Yu  QIU Quan-ying  WU Jun  WANG Juan-juan 《园艺学报》2000,16(7):585-587

AIM:To investigate the effect of berberine on IL-1 or tumour necrosis factor (TNF) induced polymorphonuclear leucocyte(PMN)-endothelium adhesion and adhesion molecules.METHODS:Based on the model of human umbilical vein endothelial cell (HUVEC), this study adopted Rose Bengal Stain, cell ELISA, immunocyto-chemical techniques to investigate the effect of berberine on PMN-endothelium adhesion and the expression of cell adhesion molecules (CAMs).RESULTS:Berberine inhibited IL-1, TNF-induced HUVEC adhesion for PMN when pretreated HUVEC and antagonised IL-1, TNF-induced upregulation of ICAM-1 on HUVEC. Meanwhile, TNF-stimulated PMN adhesion for HUVEC and CD18 upexpression on PMN was diminished in the presence of berberine.CONCLUSION: Inhibite PMN-endothelium adhesion by downregulating the CAMs expression to inhibite PMN migration across endothelium is one of the mechanisms of antiinflammation of berberine.  相似文献   

Study on the relationship between melatonin and hepatic encephalopathy     

WU Wan-chun  ZHANG Guo-zheng  WANG Yu-xiang  HE Chi-yi 《园艺学报》2000,16(9):845-847

AIM:To explore the relationship between change of serum melatonin (MT) and pathogenesis of hepatic encephalopathy (HE). METHODS: Changes of MT level in sera of cirrhosic patients with HE and without HE were determined by ELISA, normal serum served as control. The change of serum MT level in exacerbation and remission in HE was also determined.RESULTS:MT level in patients with HE was higher than that withour HE (P＜0.01). MT levels of both groups were higher than that of normal group (P＜0.01). They were (308.53±59.07) ng/L, (139.85±34.59)ng/L,(77.73±28.41)ng/L, respectively. Serum MT level in exacerbation was higher than that in remission (P＜0.01), they were (301.52±66.42)ng/L and (147.81±23.31) ng/L, respectively. CONCLUSION: The elevation of MT content in sera may be closely related to the onset of hepatic coma.  相似文献   

Rapamycin promotes repair of neurovascular units after ischemic stroke     

WU Zhi-yuan  DENG Yi-hao  ZHANG Yong-jie  HE Hong-yun 《园艺学报》2000,36(9):1709-1714

Neurovascular unit （NVU） is composed of neurons, endothelial cells, pericytes, astrocytes, macrophages/microglia, smooth muscle cells, extracellular matrix, etc, and plays vital roles in maintenance of the normal brain tissue function and cellular homeostasis. Cerebral ischemia caused by stroke can harm various components of NVU. Repairing damaged NVU and maintaining its structural and functional integrity are an effective way to improve stroke treatment. Resent research has found that rapamycin not only enhances autophagy of neurons but also effectively repairs the injuried components of NVU , thereby reducing neural damage after stroke. In this paper, the mechanisms of rapamycin underlying the repair of injured NVU after ischemic stroke is reviewed to provide guidance for its application in the treatment of stroke.  相似文献   

海水浇灌对不同品种火龙果扦插苗生长和若干生理指标的影响     

雷菲  解钰  张文  肖彤斌  符传良  张冬明  吴宇佳  谢良商 《热带作物学报》2017,38(6):1052-1057

为明确海水胁迫对火龙果生长生理的影响,以不同品种火龙果(‘帝龙’、‘红龙’、‘紫蜜龙’和‘白龙’)为研究对象,研究了海水(浓度为0、10%、20%和30%)浇灌下不同品种火龙果生长指标和生理特征的变化。结果表明:(1)海水浇灌下4个品种火龙果地上部鲜重均表现为减小趋势,但10%海水浇灌可显著增加‘帝龙’的株高增加量。(2)与淡水相比,海水浇灌下4个品种火龙果地上部和根部K+/Na+均呈不同程度下降趋势,当海水浓度为30%时,与其他3个品种相比,‘帝龙’地上部和根部K+/Na+下降幅度最小。(3)4个品种火龙果地上部可溶性糖含量、脯氨酸含量、CAT活性和SOD活性随海水浓度增加均呈先上升后下降趋势。其中,20%海水处理下,‘帝龙’可溶性糖含量、脯氨酸含量和CAT活性上升幅度均高于其他品种。可见,低浓度海水胁迫下,火龙果表现出一定耐盐性,且‘帝龙’耐盐性相对较好。  相似文献   

2株毒死蜱降解菌的分离鉴定及其混合降解特性研究     

李怡  武春媛  李玮  李勤奋 《热带作物学报》2017,(8):1512-1518

从生产毒死蜱农药厂采集的活性污泥中分离筛选得到2株降解效率较高的毒死蜱降解菌,命名为D1、D3,根据表型特征、生理生化特性和16 S rRNA基因序列相似性分析,将其鉴定为苍白杆菌属(Ochrobactrum sp.)和副球菌属(Paracoccus sp.)细菌。2株菌以最佳配比(1∶1.25)混合施用时,与单菌相比,毒死蜱降解效率可提高12%~26%;以混合菌株为研究对象,发现其对毒死蜱最适降解温度为30℃,最适降解pH值为7.0,最适NaCl浓度为0.5%;混合菌株施入土壤后,可保持较高的定殖能力和降解效率。  相似文献