全文获取类型
收费全文 | 20495篇 |
免费 | 1309篇 |
国内免费 | 1808篇 |
专业分类
林业 | 1426篇 |
农学 | 976篇 |
基础科学 | 987篇 |
2013篇 | |
综合类 | 10517篇 |
农作物 | 1724篇 |
水产渔业 | 842篇 |
畜牧兽医 | 2729篇 |
园艺 | 1557篇 |
植物保护 | 841篇 |
出版年
2024年 | 144篇 |
2023年 | 420篇 |
2022年 | 975篇 |
2021年 | 905篇 |
2020年 | 839篇 |
2019年 | 853篇 |
2018年 | 554篇 |
2017年 | 970篇 |
2016年 | 666篇 |
2015年 | 961篇 |
2014年 | 1119篇 |
2013年 | 1318篇 |
2012年 | 1670篇 |
2011年 | 1730篇 |
2010年 | 1637篇 |
2009年 | 1495篇 |
2008年 | 1454篇 |
2007年 | 1387篇 |
2006年 | 1257篇 |
2005年 | 950篇 |
2004年 | 619篇 |
2003年 | 357篇 |
2002年 | 371篇 |
2001年 | 395篇 |
2000年 | 357篇 |
1999年 | 126篇 |
1998年 | 14篇 |
1997年 | 9篇 |
1996年 | 7篇 |
1995年 | 9篇 |
1994年 | 8篇 |
1993年 | 3篇 |
1992年 | 7篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1988年 | 2篇 |
1986年 | 1篇 |
1981年 | 3篇 |
1965年 | 1篇 |
1962年 | 3篇 |
1956年 | 5篇 |
1955年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 593 毫秒
41.
42.
43.
44.
以东魁杨梅为试材,研究简易自发气调贮藏(MA)和充氮气调贮藏(NA)条件下,杨梅贮藏小环境中气体成分的变化和对贮藏效果的影响。结果表明:在MA中,乙烯浓度在2d达到高峰;O2前4d浓度急剧下降,后维持在2.48%至1.78%;CO2一直呈上升趋势,试验过程浓度提高了124.3%。在NA中,乙烯高峰出现时间明显推迟、峰高降低;O2浓度维持在2.28%-1.82%,CO2变化与MA相似,浓度提高了131.3%。MA和NA均有效延缓杨梅鲜果的软化变质,减缓果实硬度和总糖的下降速率。贮藏至16d,NA和MA中果实变质率分别比对照下降31.6%和22.5%,硬度比对照增加25.93%和21.98%,总糖含量处理与对照无明显差异。 相似文献
45.
以免疫信息学和反向疫苗学为理论根据,利用RT-PCR法获得了猪水泡病病毒(SVDV)HK/70株的基因组序列并测序,应用生物信息学相关软件和方法,对SVDV结构蛋白的二级结构的不同方面及其抗原特异性淋巴细胞表位进行了预测,综合评价了SVDV结构蛋白的抗原特异性细胞表位,并计算出一些潜在的抗原位点。结果表明,VP1蛋白含有的细胞优势抗原表位最多,但其他结构蛋白也含有抗原特异性淋巴细胞表位,有的甚至有可能成为优势抗原表位或对优势抗原表位有协同作用。 相似文献
46.
采用盆栽和小区试验的方法,经3年试验发现,坡耕地土壤结皮对作物的出苗、生长、产量和生物量等均有不同程度的影响。小麦出苗率、苗高、最大苗高、产量和生物量分别降低了22.06%、4.60%、6.86%、1.16-9.84%和0.74-6.98%;结皮虽对玉米出苗影响不大,但延长了出苗期,使产量和生物量降低了5.66—9.17%和7.17—11.25%,而且随结皮厚度、强度和地表坡度的增加,其影响更为明显。因此,通过增加地面覆盖和雨后人工耕锄以消除结皮,增加产量和保持水土。 相似文献
47.
AIM:To explore the effects of PMA(phorbol-12-myristate-13-acetate, a tumor promoter, mimicking the action of diacylglycerol on PKC)and laminin on the adhesion and the proliferation of human hepatocellular carcinoma cells, and provide a new clue to liver cancer treatment.METHODS:Human hepatocellular carcinoma cell line(BEL-7402)was used to identify the endogenous laminin and protein kinase C-α(PKC-α) expression, and the effects of laminin and PMA on the adhesion and the proliferation were also investigatedin vitro.RESULTS:By the effect of exogenous laminin, human hepatocellular carcinoma cell (BEL-7402) possessed endogenous laminin expression and increased the adhesion and the proliferation, which was showed the synergistic action by the effect of PMA in combination. By the action of PMA alone, the proliferation and the PKC-α expression increased by exogenous laminin were decreased, and the adhesion and the endogenous laminin expression were increased.CONCLUSIONS:The finding suggested that the adhesion and the proliferation of human hepatocellular carcinoma cell were closely related to the effects of endogenous or exogenous laminin, which were associated with cPKC-α activity. Therefore, the application of anti-laminin antibody in combination with PKC antagonist might be a new clue to find out the therapy for liver cancer. 相似文献
48.
49.
AIM:To examine the effect of external counterpulsation(ECP) on renin-angiotensin system(RAS) and hemodynamics in dogs with myocardial ischemia and their relationship. METHODS:Acute myocardial ischemia was induced by occluding the left anterior descending of the dogs. The local renin activity, angiotensin Ⅱ(AngⅡ) level, angiotension-converting enzyme(ACE) activity were tested by biochemical methods and hemodynamics was recorded by a 8-channel physiological recorder.RESULTS:Ischemia could actiivate renin,ACE and AngⅡ in cardiovasculature and ECP reduced them except for renin in ischemic myocardium. Ischemia also could activate RAS in lung and kidney, which play an important role on circulating RAS, and ECP reduced them. Furthermore, ECP could improve hemodynamics and there existed a close relationship between local AngⅡlevel and hemodynamics. CONCLUSION:ECP can reduce local RAS and improve hemodynamics in dogs with myocardial ischemia, which might be one of mechanisms underlying the protective effect of ECP on ischemic myocardium. 相似文献
50.
AIM:To investigate the effect of Se-containing spirulina phycocyanin (Se-SPC) on liver injury of mice induced by carbon tetrachloride (CCl4). METHODS:The mouse model was conducted by intragastric feeding with 2% CCl4 oil for three times, meanwhile Se-SPC, spirulina phycocyanin (SPC) and Na2SeO3 were injected (ip) to various groups for 7 days. Then selenium (Se), glutathione peroxidase (GPx), superoxide dismutase (SOD), alanine aminotransferase (ALT), malondiaoldehyde (MDA) and nitric oxide (NO) levels in blood and liver were measured. RESULTS:The level of Se,GPx and SOD activities were obviously higher(P<0.05)but ALT activity,MDA and NO2-/NO3- levels were remarkably lower(P<0.05)in Se-SPC treated groups than those in CCl4 groups,and effects of high dose Se-SPC on Se,GPx,MDA and NO2-/NO3- were even more significant(P<0.01).Under the same dose of Se or protein,effects of all selected targets in Se-SPC groups were more efficient than those in SPC groups and inorganic-Se groups.Furthermore,Se levels had a positive correlation with GPx activity(r=01705),which had negative correlation with levels of MDA,NO2-/NO3- and ALT(r=-0.629,r=-0.336,r=-0.457,respectively), and positive correlations between ALT activity and MDA or NO2-/NO3- level were found (r=0.519,r=0.641). CONCLUSION:These results indicated that Se-SPC may attenuate liver injury of mice induced by CCl4 through its anti-inflammatory action and enhancing selenoenzyme expression. 相似文献