Herstellung polyklonaler Antikörper in Hühnern unter Ausnutzung des immunologischen Gedächtnisses am Beispiel des Tabakmosaikvirus (TMV)     

S. Messerschmid  Prof. Dr. S. Sarkar 《Journal of pest science》1993,66(8):145-147

Zusammenfassung In einem Huhn wurden polyklonale Antikörper gegen TMV produziert. Da, um das immunologische Gedächtnis optimal auszunutzen, die Spanne zwischen erster und zweiter Immunisierung größer sein sollte als dies üblicherweise bei phytopathogenen Viren gehandhabt wird, erfolgte die zweite Immunisierung 10 Wochen nach der ersten. Bei diesem langen zeitlichen Abstand konnte nach der zweiten Immunisierung bei den Antikörpern aus dem Eidotter ein Titter von 80000 erzielt werden, nach der dritten Immunisierung stieg der Titer auf 160000. Die Antikörper, die man aus dem Blutserum gewann (Titer: 40000), konnte man auch erfolgreich im ELISA einsetzen.

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Raising polyclonal antibodies against TMV in a hen utilizing the immunological memory

Polyclonal antibodies were raised against TMV in a hen. According to the immunological theory that in order to get a good immunological memory the time interval between the first and the second immunization should be larger than mostly practised in the case of plant viruses, the second immunization was undertaken 10 weeks after the first. Using this long interval a titer of 80000 could be achieved for antibodies from egg yolk after the second immunization. After the third immunization the titer increased up to 160000. The antibodies from the blood serum (titer: 40000) could also be used successfully for the ELISA.

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Mit einer Abbildung und einer Tabelle  相似文献   

Isolation of two cDNAs encoding MHC-DQA1 and -DQA2 from the water buffalo, Bubalus bubalis     

Saket K. Niranjan  Sitangsu M. Deb  Arjava Sharma  Abhijit Mitra  Subodh Kumar   《Veterinary immunology and immunopathology》2009,130(3-4):268-271

In the present study, we explored structural and functional variations and possible duplication of the major histocompatibility complex (MHC)-DQA gene in water buffalo (Bubalus bubalis). Two cDNA sequences, amplified from one individual water buffalo, were designated as Bubu-DQA1 (DQA*0101) and -DQA2 (DQA*2001). The percentage of nucleotide and amino acid similarity between Bubu-DQA1 and -DQA2 revealed that these sequences display more similarity to alleles of respective DQA1 and DQA2 genes from other ruminant species than to each other. The phylogenetic analysis also revealed a considerably larger genetic distance between these two genes than between homologous genes from other species. The larger genetic distance between DQA*0101 and DQA*2001, and the presence of different bovine DQA putative locus specific amino acid motifs, suggests these sequences are non-allelic. This finding is consistent with DQA gene duplication in other ruminants.  相似文献   

Effect of Anserine and Carnosine on Sperm Motility in the Japanese Quail     

Prodip Kumar Sarkar  Ai Egusa  Mei Matsuzaki  Tomohiro Sasanami 《The Journal of Poultry Science》2021,58(3):186

Sperm motility is considered as one of the most important traits for successful fertilization, but the motility of an ejaculated sperm decreases with time when stored as liquid. It is reported that seminal plasma serves as a nutrient rich medium for sperm and plays an important role in sperm motility and its fertilization ability. Several studies have reported that imidazole dipeptides such as anserine and carnosine affect sperm motility and its fertilization ability in mammals. In this study, we report the presence of anserine and carnosine in the male reproductive tract of the Japanese quail. Abundant levels of anserine (44.46 µM) and carnosine (41.75 µM) were detected in the testicular fluid and seminal plasma respectively using the amino acid analyzer; however, seminal plasma solely contained carnosine. When the ejaculates were incubated with anserine or carnosine, we found that both the dipeptides improve sperm motility parameters such as straight line velocity, curvilinear velocity, average path velocity and amplitude of lateral head displacement after in vitro sperm storage at 15°C. These results indicate that imidazole dipeptides are present in the male reproductive tract and may improve sperm quality during in vitro sperm storage in the liquid states.  相似文献   

Herstellung polyklonaler Antik?rper in Hühnern unter Ausnutzung des immunologischen Ged?chtnisses am Beispiel des Tabakmosaikvirus (TMV)     

S. Messerschmid  S. Sarkar 《Anzeiger für Sch?dlingskunde》1993,66(8):145-147

In einem Huhn wurden polyklonale Antikörper gegen TMV produziert. Da, um das immunologische Gedächtnis optimal auszunutzen, die Spanne zwischen erster und zweiter Immunisierung größer sein sollte als dies üblicherweise bei phytopathogenen Viren gehandhabt wird, erfolgte die zweite Immunisierung 10 Wochen nach der ersten. Bei diesem langen zeitlichen Abstand konnte nach der zweiten Immunisierung bei den Antikörpern aus dem Eidotter ein Titter von 80000 erzielt werden, nach der dritten Immunisierung stieg der Titer auf 160000. Die Antikörper, die man aus dem Blutserum gewann (Titer: 40000), konnte man auch erfolgreich im ELISA einsetzen.Polyclonal antibodies were raised against TMV in a hen. According to the immunological theory that in order to get a good immunological memory the time interval between the first and the second immunization should be larger than mostly practised in the case of plant viruses, the second immunization was undertaken 10 weeks after the first. Using this long interval a titer of 80000 could be achieved for antibodies from egg yolk after the second immunization. After the third immunization the titer increased up to 160000. The antibodies from the blood serum (titer: 40000) could also be used successfully for the ELISA.

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Mit einer Abbildung und einer Tabelle  相似文献   

Postnatal persistence of foetal haemoglobin in yaks   总被引：2，自引：0，他引：2  

Sarkar M  Pal RN  Das DN  Mondal DB  Mohanty TK  Pattanaik S 《Australian veterinary journal》1999,77(3):190-190

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Flufenoxuron, an acylurea insect growth regulator, alters development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) by modulating levels of chitin, soluble protein content, and HSP70 and p34 in the larval tissues     

Shaila Salokhe  Angshuman Sarkar  Samindranath Mukherjee 《Pesticide biochemistry and physiology》2006,85(2):84-90

The effect of sublethal concentrations, 0.00141% (LC₂₀), 0.00251% (LC₃₀), and 0.0036% (LC₄₀) of a dispersible formulation of an acylurea insect growth regulator, flufenoxuron (Cascade) on certain biochemical parameters in the larvae of Tribolium castaneum was investigated. When neonates were fed on diet treated with sublethal concentrations for 24 h, it was observed that at all concentrations tested, there was a significant reduction in chitin content on the 15th day of development. Total soluble protein content at LC₂₀ and LC₃₀ decreased with increasing age of the larvae. At LC₂₀ and LC₄₀ concentrations there was a progressive increase in the protein: chitin ratio as a function of increase in age of the larvae. SDS-PAGE analysis of the larval tissue extracts indicated gross quantitative changes in some of the protein bands (MW 50-97 kDa). Western blot analysis revealed significant increase in the level of HSP70 in the extracts of larvae fed on LC₃₀ treated diet, on the 7th and 10th day of development in the decreasing order. Conversely, a significant decrease in the hyper-phosphorylated form of p34^cdc2 kinase due to flufenoxuron treatment indicating modulation of cell cycle regulation was observed. Thus, sublethal concentrations of flufenoxuron alter expression of developmentally regulated proteins, HSP70 and p34^cdc2 and chitin formation in a stage-specific manner thereby resulting developmental abnormalities in T. castaneum.  相似文献   

Hyperplasic and hypertrophic growth in brain, liver and muscle of undernourished suckled pigs     

G A Lodge  N K Sarkar  D W Friend 《Journal of animal science》1977,45(6):1346-1352

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Persistence and metabolism of imidacloprid in different soils of West Bengal     

Sarkar MA  Roy S  Kole RK  Chowdhury A 《Pest management science》2001,57(7):598-602

A laboratory experiment was performed to study the persistence of imidacloprid from two formulations (Confidor 200 g litre-1 SL and Gaucho 700 g kg-1 WS), and its metabolism in three different soils (Gangetic alluvial soil of Kalyani, lateritic soil of Jhargram and coastal alkaline soil of Canning) of West Bengal following application at 0.5 kg and 1.0 kg AIha-1. Dissipation of imidacloprid in soil followed first-order kinetics and DT50 values ranged from 28.7 to 47.8 days. The shortest half-lives (28.7 and 35.8 days) were observed in the lateritic soil of Jhargram for both liquid and powder formulations. The formation of two metabolites of imidacloprid, imidacloprid-urea and imidacloprid-olefin, was first detected on day 30 of degradation at 28 (+/- 1) degrees C in all three soils.  相似文献   

Transcriptional profile of endometrial TLR4 and 5 genes during the estrous cycle and uterine infection in the buffalo (Bubalus bubalis)     

Ganesan Ajevar  Sankar Muthu  Mihir Sarkar  Harendra Kumar  Goutam Kumar Das  Narayanan Krishnaswamy 《Veterinary research communications》2014,38(2):171-176

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A functional screen for the type III (Hrp) secretome of the plant pathogen Pseudomonas syringae     

Guttman DS  Vinatzer BA  Sarkar SF  Ranall MV  Kettler G  Greenberg JT 《Science (New York, N.Y.)》2002,295(5560):1722-1726

Type III secreted "effector" proteins of bacterial pathogens play central roles in virulence, yet are notoriously difficult to identify. We used an in vivo genetic screen to identify 13 effectors secreted by the type III apparatus (called Hrp, for "hypersensitive response and pathogenicity") of the plant pathogen Pseudomonas syringae. Although sharing little overall homology, the amino-terminal regions of these effectors had strikingly similar amino acid compositions. This feature facilitated the bioinformatic prediction of 38 P. syringae effectors, including 15 previously unknown proteins. The secretion of two of these putative effectors was shown to be type III--dependent. Effectors showed high interstrain variation, supporting a role for some effectors in adaptation to different hosts.  相似文献