Long-term separate and combined effects of environmental hypercapnia and hyperoxia in Atlantic salmon (Salmo salar L.) smolts   总被引：2，自引：1，他引：2  

Camilla Diesen Hosfeld  Annhild Engevik  Ted Mollan  Torleif Markussen Lunde  Rune Waagb  Anne Berit Olsen  Olav Breck  Sigurd Stefansson  Sveinung Fivelstad 《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):146-153

Atlantic salmon (Salmo salar L.) parr (mean start weight 50 g) were reared in freshwater (FW) and exposed to three levels of oxygen saturation measured in effluent water; control group (93% O₂, LO₂), medium (111% O₂, MO₂) and high (123% O₂, HO₂). Further three groups were exposed to similar water oxygen levels in combination with elevated carbon dioxide levels (17–18 mg L^– 1 CO₂), named LO₂–CO₂, MO₂–CO₂ and HO₂–CO₂, respectively. The experiment was run in duplicate tanks for 42 days, and the fish were subsequently transferred to the same seawater (SW) regime for 45 days for an assessment of post-smolt growth. As a consequence of the CO₂ addition, tank pH levels in the FW period were reduced from 6.7 to 5.9 for the hypercapnia groups compared to for the normcapnia groups. Water temperature in FW ranged between 6.4 and 9.0 °C. Citrate was added to the water to complex labile aluminium.In the CO₂ groups observed ventilation frequencies were significantly increased compared to the control (p < 0.05). This difference declined towards the end of the FW period, suggesting acclimation to elevated CO₂. The degree of oxygenation appeared to contribute to the acclimation as the lowest mean ventilation frequency on day 36 was found in the HO₂–CO₂ group and the highest in the LO₂–CO₂ group. Lower plasma chloride and sodium levels were observed in the CO₂ groups relative to the respective oxygenation groups during the FW period, while plasma chloride and sodium levels were normalised to equal levels for all groups after 44 days in SW. No significant differences were found among treatments for blood concentrations of red blood cells, haemoglobin, potassium and glucose during the experiment.By termination of the FW period, the HO₂ group had significantly higher body weight than all other groups (p < 0.05), with specific growth rate significantly higher than the CO₂ groups (p < 0.05). Further, the condition factor was significantly lower in all the CO₂ groups at the end of the FW period compared to the control and normcapnia groups (p < 0.05). Although variable among replicates, occurrence of nephrocalcinosis was 10 times higher in the hypercapnia groups than in the control and normcapnia groups. Mortality was negligible (< 2.0%) during the trial, and most of the mortality occurred following SW transfer.  相似文献   

Oogenesis and lipids in gonad and liver of daubed shanny (Leptoclinus maculatus) females from Svalbard waters     

Murzina SA  Meyer Ottesen CA  Falk-Petersen S  Hop H  Nemova NN  Poluektova OG 《Fish physiology and biochemistry》2012,38(5):1393-1407

Oocyte and liver histomorphology of the daubed shanny (Leptoclinus maculatus) from Isfjorden and Kongsfjorden in Svalbard were investigated during three Arctic seasons: summer (July), autumn (October) and winter (April). Three oocyte developmental phases were observed: primary growth phase, secondary growth phase and maturation phase. We observed four different developmental stages: (1) perinucleolus stage with cortical alveolus formation, (2) lipid droplets formation, (3) vitellogenesis stage and (4) maturation. Late maturation stage of oocytes in the ovaries was from the autumn season. Females accumulated lipids in liver (up to 35.2?% dw) and deposited large amounts of lipids into gonads (up to 52.2?% dw) during maturation. Lipid classes in female gonads changed seasonally, with relative increase in cholesterol during summer and depletion of storage lipids (triacylglycerols and wax esters/cholesterol esters) during the winter. Lipid composition in liver changed during oocyte development and spawning, as neutral lipids were transferred to developing oocytes during summer to autumn. During winter, storage lipids were depleted during starvation. Based on the increase in gonadosomatic index (GSI) with length and observed maturation stages, females seem to mature at a length of about 125-130?mm. The GSI and hepatosomatic index of large females sampled in autumn (September-October) were significantly higher than for females in late winter (April) and spring (May). These results indicate that spawning takes place during winter in Isfjorden and that energy reserves stored in the liver are utilized by females during gonadal development and reproduction.  相似文献   

Cholinesterase Levels in Blood Plasma and Erythrocytes from Calves,Normal Delivering Cows and Cows Suffering from Parturient Paresis     

Kristina Forslund  Camilla Bjrkman  Maivor Abrahamsson 《Acta veterinaria Scandinavica》1983,24(2):185

Plasma cholinesterase (pChE) levels and erythrocyte acetylcholinesterase (eAChE) levels were studied in 6 cows before, during and after parturition (Group I), their calves (Group II), 38 cows suffering from parturient paresis (Group III) and 14 newly delivered non-paretic cows (Group IV).The mean of the pChE level in Group I was 1.5 μkat/1 ± 0.20 before parturition and decreased significantly (P ≦ 0.05) to 1.2 ukat/1 ± 0.16 after parturition. The eAChE level was before parturition ≅ 140 ukat/1 and decreased to ≅ 130 μkat/1 4–5 weeks after parturition.At birth the pChE level was 12.8 ukat/1 ± 5.9 in Group II. After 4 weeks the level had decreased to 2.3 ukat/1 ±0.3. In the bull calves the pChE level started to increase when they were 6 weeks old and reached a level of 5.7 μkat/1 ± 0.6 before slaughter at 6 months of age. The heifers did not show this increase. They had a level of around 2 μkat/1 throughout the investigation. The eAChE level at birth was 119 μkat/1 and increased slowly to a level of 145 μkat/1 at 6 months. No differences between the sexes were found.The cows suffering from parturient paresis had a pChE level of 1.80 μkat/1 ± 0.30 before treatment with calcium (Ca). The level decreased significantly (P ≦ 0.001) after Ca-infusion to a level of 1.67 ukat/1 ±0.29. Group IV had a pChE level of 1.65 μkat/1 ± 0.42 at parturition. Two to 4 months later the cows that had recovered from milk fever had a level of 1.61 μkat/1 ± 0.31 and the control cows 1.66 ukat/1 ± 0.48. No differences between the groups were found for the eAChE level.The findings show that parturition influences the pChE level in cows and that sex influences the pChE level in calves between 6 weeks to at least 6 months of age. Furthermore the elevated pChE level found in the cows suffering from parturient paresis before Ca infusion may be a further sign of a disturbance in the cholinergic system with a special preference to the neuromuscular junctions.  相似文献   

A scoring system for risk assessment of the introduction and spread of bovine viral diarrhoea virus in dairy herds in Northern Italy     

Luzzago C  Frigerio M  Piccinini R  Daprà V  Zecconi A 《Veterinary journal (London, England : 1997)》2008,177(2):236-241

To support a voluntary disease control program, this study aimed to develop an integrated scoring system for the risk assessment of bovine viral diarrhoea virus (BVDV) infection in dairy herds in Northern Italy. Sixty-two dairy herds were classified according to their BVDV serological status. Farmers were interviewed using a questionnaire on potential BVDV risk factors. Scores were used to define risk levels for factors related to (1) BVDV introduction (through livestock trade, attendance of animals at shows/exhibitions and grazing common pasture), (2) within-herd spread of BVDV and (3) the results of initial serological testing. The calculated odds ratios were significant for all categories, except for livestock trade. The application of the screening test, the questionnaire and the related risk assessment showed this to be a practical approach to predicting BVDV herd status.  相似文献   

A three-year longitudinal study on the effects of a diet containing genetically modified Bt176 maize on the health status and performance of sheep     

Massimo Trabalza-Marinucci  Giorgio Brandi  Cristina Rondini  Luca Avellini  Camilla Giammarini  Silva Costarelli  Gabriele Acuti  Chiara Orlandi  Giovanni Filippini  Elisabetta Chiaradia  Manuela Malatesta  Silvia Crotti  Chiara Antonini  Giulia Amagliani  Elisabetta Manuali  Anna Rita Mastrogiacomo  Livia Moscati  Mohamed Naceur Haouet  Alberto Gaiti  Mauro Magnani   《Livestock Science》2008,113(2-3):178-190

This study shows that a diet including insect-resistant Bt176 maize, fed to 53 ewes and their progeny for 3 years, did not have adverse effects on their health or performance and that no horizontal gene transfer to ruminal microorganisms or animal tissues was detected. No differences were observed regarding performance, reproductive traits, haematological parameters, antioxidant defences, lymphocyte proliferative capacity, phagocytosis and intracellular killing of macrophages, and ruminal microbial population characteristics between control and genetically modified (GM) maize-fed animals. Immune response to Salmonella abortus ovis vaccination was more efficient in GM maize fed sheep. No modifications of histological features of tissues were found; however, cytochemical analyses of ruminal epithelium by Ki67 staining provided evidence of proliferative activation of basal cells in all GM maize-fed ewes. Preliminary electron microscopy analyses of the liver and pancreas revealed smaller cell nuclei containing increased amounts of heterochromatin and perichromatin granules in GM maize-fed lambs. Meat protein content and water loss by cooking were slightly affected by the dietary treatment. No transgenic DNA was detected in tissues, blood, and ruminal fluid or ruminal bacteria. Longitudinal studies should be included in evaluation of food safety whenever possible and sheep may be a useful animal model for toxicological assessment.  相似文献   

Application of the Neospora caninum IgG avidity ELISA in assessment of chronic reproductive losses after an outbreak of neosporosis in a herd of beef cattle.     

Camilla Bj?rkman  Milton M McAllister  Jenny Fr?ssling  Katarina N?slund  Felicia Leung  Arvid Uggla 《Journal of veterinary diagnostic investigation》2003,15(1):3-7

Point-source infections are most likely the cause for Neospora caninum-induced abortion outbreaks in cattle, whereas an increased annual abortion rate may be a consequence of vertical transmission. The aims of the present study were to examine the reproductive effects of neosporosis in a beef herd for 3 years, after a point-source outbreak and to use IgG avidity serology to examine the chronicity of infections and patterns of transmission. During the study, 76-78% of animals were seropositive for N. caninum. The pregnancy rate varied from 88% to 94%, without any reduction in the pregnancy rate of seropositive cows compared with seronegative cows. The annual abortion rate was 2.5-5.5%, and all but 1 abortion occurred in seropositive dams. The efficiency of vertical transmission was estimated to be 85%. Several calves, born to seronegative dams, were seropositive at 6-13 months of age, indicating a 22% mean annual rate of horizontal transmission. The mean avidity in seropositive cows increased from 30 during the initial outbreak to 74 after 3 years. The mode of IgG avidity was 21-40 during the initial abortion outbreak, 41-60 after 1 year, and 61-80 after 2 and 3 years. The results reveal high annual rates of both vertical and horizontal transmission of N. caninum in a herd of beef cows and provide further validation on the ability of the N. caninum IgG avidity ELISA to accurately assess the chronicity of infection.  相似文献   

The mycorrhizal fungus (Glomus intraradices) affects microbial activity in the rhizosphere of pea plants (Pisum sativum)     

Camilla WambergSøren Christensen  I. JakobsenA.K. Müller  S.J. Sørensen 《Soil biology & biochemistry》2003,35(10):1349-1357

Pea plants were grown in γ-irradiated soil in pots with and without addition of the AM fungus Glomus intraradices at sufficient N and limiting P. Depending on the growth phase of the plant presence of AM had negative or positive effect on rhizosphere activity. Before flowering during nutrient acquisition AM decreased rhizosphere respiration and number of protozoa but did not affect bacterial number suggesting top-down regulation of bacterial number by protozoan grazing. In contrast, during flowering and pod formation AM stimulated rhizosphere respiration and the negative effect on protozoa decreased. AM also affected the composition of the rhizosphere bacterial community as revealed from DNA analysis (DGGE). With or without mycorrhiza, rhizosphere respiration was P-limited on very young roots, not nutrient limited at more mature roots and C-limited at withering. This suggests changes in the rhizosphere community during plant growth also supported by changes in the bacteria (DGGE).  相似文献   

Interaction between foliar-feeding insects, mycorrhizal fungi, and rhizosphere protozoa on pea plants     

Camilla Wamberg  Sren Christensen  Iver Jakobsen 《Pedobiologia》2003,47(3):281-287

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Impact of breed on canine humeral condylar fracture configuration,surgical management,and outcome     

Carlos Sanchez Villamil CertAVP  DVM  Andrew S. J. Phillips BVetMed  MVetMed  DECVS  Camilla L. Pegram BVetMed MRes  Dan G. O'Neill MVB  BSc  MSc  PhD  Richard L. Meeson MA  VetMB  PhD  MVetMed  DECVS 《Veterinary surgery : VS》2020,49(4):639-647

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Limitations of farm management data in analyses of decadal changes in SOC stocks in the Danish soil-monitoring network     

Laura Sofie Harbo  Jørgen E. Olesen  Camilla Lemming  Bent T. Christensen  Lars Elsgaard 《European Journal of Soil Science》2023,74(3):e13379

Changes in soil organic carbon (SOC) storage in agricultural land are an important part of the Land Use, Land-Use Change and Forestry component of national greenhouse gas emission inventories. Furthermore, as climate mitigation strategies and incentives for carbon farming are being developed, accurate estimates of SOC stocks are essential to verify any management-induced changes in SOC. Based on agricultural mineral soils in the Danish soil-monitoring network, we analysed management effects on SOC stocks using data from the two most recent surveys (2009 and 2019). Between 2009 and 2019, the average increase in SOC stock was 1.2 Mg C ha⁻¹ for 0–50 cm despite a loss of 1.2 Mg C ha⁻¹ from the topsoil (0–25 cm), stressing the importance of including deeper soil layers in soil-monitoring networks. Comparing all four national surveys (1986, 1997, 2009, 2019), the mean SOC stock of mineral soils in Denmark appears stable. The change in SOC stock between 2009 and 2019 was analysed in detail in relation to management practices as reported by farmers. We found that the effects of single management factors were difficult to isolate from co-varying factors including soil parameters and that the use of farm management data to explain changes in SOC stocks observed in soil-monitoring networks appears limited. Uncertainty in SOC stock estimates also arises from low sampling frequency and statistical challenges related to regression to the mean. However, repeated stock measurements at decadal intervals still represent a benchmark for the overall development in regional and national SOC storage, as affected by actual farm management.  相似文献