Optimization of Agrobacterium-mediated transformation parameters for Melastomataceae spp. using green fluorescent protein (GFP) as a reporter     

Wilson Thau Lym Yong  Janna Ong Abdullah  Maziah Mahmood 《Scientia Horticulturae》2006

Agrobacterium-mediated transformation for both Melastoma malabathricum and Tibouchina semidecandra were optimized using green fluorescent protein (GFP) as a reporter. The binary vector pCAMBIA1304 harboring the modified green fluorescent protein (mgfp) gene driven by the CaMV 35S promoter was used. Parameters optimized were bacterial strain, bacterial concentration, pre-culture period, co-cultivation period, immersion time, acetosyringone concentration and wounding type. Results obtained were based on the percentage of GFP expression which was observed 3 days post-transformation. Agrobacterium tumefaciens strain LBA4404 and EHA105 at concentration 1 × 10⁷ cfu ml⁻¹ (OD_600nm 0.8) showed the highest virulence on M. malabathricum and T. semidecandra, respectively. Four days of pre-culture and 2 days of co-cultivation were optimum for M. malabathricum transformation, while 3 days of pre-culture and co-cultivation for T. semidecandra. Result also showed that 60 min of immersion and addition of 200 μM acetosyringone gave the highest percentage of positive transformants for both M. malabathricum and T. semidecandra. Mild wounding also significantly increased the efficiency of M. malabathricum transformation.  相似文献   

Effect of root applied 28-homobrassinolide on the performance of Lycopersicon esculentum     

B. Ali  S. HayatS. Aiman Hasan  A. Ahmad 《Scientia Horticulturae》2006

The roots of 20 days old seedlings of tomato (Lycopersicon esculentum Mill.) at the time of their transplantation, were dipped in 10⁻⁸, 10⁻⁷, 10⁻⁶ or 10⁻⁵ M of 28-homobrassinolide (HBR) for 15, 30 or 45 min and were allowed to grow in earthen pots, in a net house. The leaves of the plants, at days 30 and 60, possessed elevated quantities of nitrate reductase (NR), carbonic anhydrase (CA) and the contents of chlorophyll. The values for all the above characteristics were significantly higher than that of the water-fed control. The fruits borned at the treated plants were more in number and possessed a lower quantity of ascorbic acid than the control. Moreover, the fruits at ripening, had higher levels of lycopene and β-carotene. Among the treatments, 15 min feeding of 10⁻⁸ M HBR proved best.  相似文献   

Pyrrolizidine alkaloids from Senecio lorenthii.     

M Noorwala  F V Mohammad  V U Ahmad  B Sener  F Ergun  D Deliorman 《Fitoterapia》2000,71(5):618-620

The isolation and NMR spectra of otosenine (1) and seneciphylline (2) from Senecio lorenthii are reported.  相似文献   

Detection of African horsesickness (AHS) in recently vaccinated horses with inactivated vaccine in Qatar     

M M Hassanain  A I al-Afaleq  I M Soliman  S K Abdullah 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1990,43(1):33-35

Two 7-year old Arabian racing horses were reported to show typical AHS symptoms in Qatar and died shortly after. The horses had been vaccinated with formol inactivated vaccine approximately 10 days before the onset of the disease. Blood samples from these horses were collected and AHS virus isolated from one sample after intracerebral (i.c.) inoculation into suckling mice. The virus identity was confirmed by complement fixation test (CFT) using the virus antigen and reference type 9 of AHS virus hyperimmune serum. The serotype of the isolated virus was identified by serum neutralization test (SNT) using reference types of AHS virus. Two possibilities of the original source of this infection were suggested. The infection might be due first to the natural endemic occurrence of the virus in the country and secondly, to the presence of residual infectious virus in the inactivated vaccine.  相似文献   

Wheat Seed Presoaking for Improved Germination     

Shahbaz Ahmad  Muhammad Anwar  Hameed Ullah 《Journal of Agronomy and Crop Science》1998,181(2):125-127

Seed presoaking improves wheat germination under marginal moisture conditions. The duration of seed presoaking was studied at The University of Arid Agriculture, Rawalpindi, using 10 wheat varieties. Seed presoaking beyond 12 h does not improve germination further, and beyond 21 h, germination rate is drastically reduced.  相似文献   

Alkaline sulfite–anthraquinone (AS/AQ) pulping of wheat straw and totally chlorine free (TCF) bleaching of pulps     

Sahab Hedjazi  Othar Kordsachia  Rudolf Patt  Ahmad Jahan Latibari  Ulrike Tschirner 《Industrial Crops and Products》2009,29(1):27-36

Alkaline sulfite–anthraquinone (AS/AQ) pulping of wheat straw and totally chlorine free (TCF) bleaching of resulting pulps was investigated. Wheat straw was supplied by the Ferdosii University Experimental Station in Northeastern Iran, cleaned and chopped into about 3–5 cm long pieces. In the AS/AQ pulping experiments, the active alkali charge on oven dry wheat straw, heating time to maximum cooking temperature, AQ dose and liquor to straw ratio were kept constant at 16%, 60 min, 0.1% and 4/1, respectively. The alkali ratio, Na₂SO₃/NaOH, calculated as NaOH, was varied from 20/80 to 80/20. Cooking time at maximum temperature was varied from 30 to 90 min and maximum cooking temperature was adjusted between 152 and 160 °C. Reference soda and soda/AQ pulps were produced.AS/AQ and soda/AQ pulps with kappa numbers below 15 were produced easily. Cooking of corresponding soda pulps under comparable conditions stopped at a kappa number of 25. The characteristics of unbleached AS/AQ, soda/AQ and soda pulps were compared. AS/AQ and soda/AQ pulps had similar tensile strength but tear was in favor to AS/AQ. The latter had much higher yield and brightness.TCF bleaching of AS/AQ pulp was performed in O Q (OP) or O Q (OP) P sequences. Oxygen delignification (O) resulted in a delignification degree of approximately one third. Followed by a chelating treatment (Q) and a subsequent (OP) stage under drastic conditions a brightness of 82.7% ISO was achieved in an O Q (OP) sequence. The sequence O Q (OP) P, with 2% H₂O₂ in the final P stage, improved brightness from 77.2% ISO after the OP stage to 83.6% ISO. Bleaching led to only small losses in tensile and burst strength but enhanced tear strength. Short beating in a Jokro mill was sufficient to attain good pulp strength. The results of this study indicate that AS/AQ pulping and TCF bleaching is a convincing alternative to produce high-quality pulp from wheat straw for writing and printing paper.  相似文献   

The effect of praziquantel on the hepatic activities of some drug-metabolizing enzymes in rabbits experimentally infected with Schistosomabovis     

H.A. Elsheikh  W.M. Kheir  & A.S. Abdullah 《Journal of veterinary pharmacology and therapeutics》1997,20(5):411-414

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Über das Artenspektrum Manna erzeugender Aphiden     

M. B. Al-Aswad  M. Ahmad  D. M. Sabir 《Journal of pest science》1977,50(11):164-165

Identification of Manna excreting Aphids Up to now the origin of Manna in northern Iraq has not been known distinctly. As producers of the sweet excretions called Manna on oak trees were identified the following species of Aphididae:Aphis fabae Scop.,A. gossypii Glov.,Chromaphis juglandicola Kalt.,Myzocallis castanicola Bak.,Tuberculatus annulata Htg.,T. moerickei Hrl. andThelaxes sp.  相似文献   

Alkaloids of Zanthoxylum budrunga     

Ahmad MU  Rahman MA  Huq E  Chowdhury R 《Fitoterapia》2003,74(1-2):191-193

Pseudophrynamine, lunacridine and the new compound 2-(2',4',6'-trimethyl-heptenyl)-4-quinozolone were isolated from the leaves of Zanthoxylum budrunga.  相似文献   

Antinociceptive diterpene from Euphorbia decipiens     

Ahmad VU  Hussain H  Bukhari IA  Hussain J  Jassbi AR  Dar A 《Fitoterapia》2005,76(2):230-232

One myrsinol-type diterpene ester (1) isolated from Euphorbia decipiens was evaluated for analgesic activity in the acetic acid induced writhing test in mice. Different dose (5-20 mg/kg i.p.) of the compound showed significant antinociceptive activity, which was comparable to standard analgesic drugs, aspirin and ibuprofen (100 mg/kg i.p.).  相似文献