Rapid and high-precision marker assisted backcrossing to introgress the <Emphasis Type="Italic">SUB1</Emphasis> QTL into BR11, the rainfed lowland rice mega variety of Bangladesh     

K. M. Iftekharuddaula  M. A. Newaz  M. A. Salam  H. U. Ahmed  M. A. A. Mahbub  E. M. Septiningsih  B. C. Y. Collard  D. L. Sanchez  A. M. Pamplona  D. J. Mackill 《Euphytica》2011,178(1):83-97

Flooding is one of the major hazards of rice production for the rainfed lowland rice ecosystem, and tolerant cultivars are urgently needed to help protect farmers from submergence damage. A quick and efficient strategy was implemented to introgress SUB1, a major QTL for submergence tolerance, into a rainfed lowland mega variety BR11 of Bangladesh by only two backcrosses and one selfing generation. In marker-assisted backcrossing (MABC), one tightly-linked simple sequence repeat (SSR) and two gene-based markers, four flanking SSR and 116 background SSR markers were used for foreground, recombinant and background selection, respectively, in backcrosses between a SUB1 donor IR40931-33-1-3-2 and BR11. BR11-Sub1, identified in a BC₂F₂ plant, possessed BR11 type SSR alleles on all fragments analyzed except the SUB1 QTL. The introgression size in BR11-Sub1 was 800 Kb indicating approximately 99.8% identity to BR11. BR11-Sub1 along with other introgression lines showed submergence tolerance similar to the tolerant parent. Yield, yield-component parameters and grain physico-chemical properties showed successful recovery of the BR11 traits in BR11-Sub1, with yield potential ranging from 5.2 to 5.6 t/ha, not significantly different from the recurrent parent mega variety BR11. Producing a large number (~1000) of backcross F₁ plants was considered essential to achieve recombination on both sides of the gene, limiting linkage drag with only two backcrosses. A large number of background markers ensured proper recovery of the recurrent parent genome in the BC₂F₂ generation. The study demonstrates a rapid and highly precise strategy to introgress a major QTL by BC₂F₂ generation into a modern rice variety using an unadapted donor. The variety can replace BR11 on more than 2 million of ha in Bangladesh and provide major increases in rice production.  相似文献   

Soil salinity related to physical soil characteristics and irrigation management in four Mediterranean irrigation districts   总被引：1，自引：0，他引：1  

R. Aragüés  V. UrdanozM. Çetin  C. KirdaH. Daghari  W. LtifiM. Lahlou  A. Douaik 《Agricultural Water Management》2011,98(6):959-966

Irrigated agriculture is threatened by soil salinity in numerous arid and semiarid areas of the Mediterranean basin. The objective of this work was to quantify soil salinity through electromagnetic induction (EMI) techniques and relate it to the physical characteristics and irrigation management of four Mediterranean irrigation districts located in Morocco, Spain, Tunisia and Turkey. The volume and salinity of the main water inputs (irrigation and precipitation) and outputs (crop evapotranspiration and drainage) were measured or estimated in each district. Soil salinity (EC_e) maps were obtained through electromagnetic induction surveys (EC_a readings) and district-specific EC_a-EC_e calibrations. Gravimetric soil water content (WC) and soil saturation percentage (SP) were also measured in the soil calibration samples. The EC_a-EC_e calibration equations were highly significant (P < 0.001) in all districts. EC_a was not significantly correlated (P > 0.1) with WC, and was only significantly correlated (P < 0.1) with soil texture (estimated by SP) in Spain. Hence, EC_a mainly depended upon EC_e, so that the maps developed could be used effectively to assess soil salinity and its spatial variability. The surface-weighted average EC_e values were low to moderate, and ranked the districts in the order: Tunisia (3.4 dS m⁻¹) > Morocco (2.2 dS m⁻¹) > Spain (1.4 dS m⁻¹) > Turkey (0.45 dS m⁻¹). Soil salinity was mainly affected by irrigation water salinity and irrigation efficiency. Drainage water salinity at the exit of each district was mostly affected by soil salinity and irrigation efficiency, with values very high in Tunisia (9.0 dS m⁻¹), high in Spain (4.6 dS m⁻¹), moderate in Morocco (estimated at 2.6 dS m⁻¹), and low in Turkey (1.4 dS m⁻¹). Salt loads in drainage waters, calculated from their salinity (EC_dw) and volume (Q), were highest in Tunisia (very high Q and very high EC_dw), intermediate in Turkey (extremely high Q and low EC_dw) and lowest in Spain (very low Q and high EC_dw) (there were no Q data for Morocco). Reduction of these high drainage volumes through sound irrigation management would be the most efficient way to control the off-site salt-pollution caused by these Mediterranean irrigation districts.  相似文献   

A map of the interactome network of the metazoan C. elegans   总被引：1，自引：0，他引：1  

Li S  Armstrong CM  Bertin N  Ge H  Milstein S  Boxem M  Vidalain PO  Han JD  Chesneau A  Hao T  Goldberg DS  Li N  Martinez M  Rual JF  Lamesch P  Xu L  Tewari M  Wong SL  Zhang LV  Berriz GF  Jacotot L  Vaglio P  Reboul J  Hirozane-Kishikawa T  Li Q  Gabel HW  Elewa A  Baumgartner B  Rose DJ  Yu H  Bosak S  Sequerra R  Fraser A  Mango SE  Saxton WM  Strome S  Van Den Heuvel S  Piano F  Vandenhaute J  Sardet C  Gerstein M  Doucette-Stamm L  Gunsalus KC  Harper JW  Cusick ME  Roth FP  Hill DE  Vidal M 《Science (New York, N.Y.)》2004,303(5657):540-543

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Analysis of the Offspring Sex Ratio of Chicken by Using Molecular Sexing     

FENG Yan-ping GONG Yan-zhang Nabeel Ahmed Affara PENG Xiu-li YUAN Jin-feng ZHAO Rui-Xia Mohammed Yusuf Osman Jeffer ZHANG Shu-jun 《中国农业科学(英文版)》2006,5(7):545-549

The overall sex ratio of offspring （dead embryos and hatch chicks） from all the fertilized eggs of 140 hens collected for 30 days was studied using duplex PCR of certain fragments of sex chromosomes. Additional 894 dead embryos over a period of 21 days of incubation were also investigated to verify the sex ratio of the dead embryos. The sex of the early dead embryos was identified using this molecular sexing technique. The sex ratio of the hatch chicks and the total offspring of the hens investigated in this experiment did not differ from the expected sex ratio （i.e., 1：1）., However, the number of female dead embryos was significantly more than that of males. The data indicated that the different physiologic function of males and females contributed to female-biased mortality during incubation. It was also found by further analysis that the sex ratios of the offspring of some hens were significantly biased to female or male over the period investigated, which suggested that the sex ratio of offspring might be influenced by the maternal condition to some degrees.  相似文献   

Ultrafast electron crystallography of interfacial water     

Ruan CY  Lobastov VA  Vigliotti F  Chen S  Zewail AH 《Science (New York, N.Y.)》2004,304(5667):80-84

We report direct determination of the structures and dynamics of interfacial water on a hydrophilic surface with atomic-scale resolution using ultrafast electron crystallography. On the nanometer scale, we observed the coexistence of ordered surface water and crystallite-like ice structures, evident in the superposition of Bragg spots and Debye-Scherrer rings. The structures were determined to be dominantly cubic, but each undergoes different dynamics after the ultrafast substrate temperature jump. From changes in local bond distances (OH.O and O.O) with time, we elucidated the structural changes in the far-from-equilibrium regime at short times and near-equilibration at long times.  相似文献   

Comment on "Arsenic Mobility and Groundwater Extraction in Bangladesh" (II)     

van Geen A  Zheng Y  Stute M  Ahmed KM 《Science (New York, N.Y.)》2003,300(5619):584; author reply 584

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4D visualization of transitional structures in phase transformations by electron diffraction     

Baum P  Yang DS  Zewail AH 《Science (New York, N.Y.)》2007,318(5851):788-792

Complex systems in condensed phases involve a multidimensional energy landscape, and knowledge of transitional structures and separation of time scales for atomic movements is critical to understanding their dynamical behavior. Here, we report, using four-dimensional (4D) femtosecond electron diffraction, the visualization of transitional structures from the initial monoclinic to the final tetragonal phase in crystalline vanadium dioxide; the change was initiated by a near-infrared excitation. By revealing the spatiotemporal behavior from all observed Bragg diffractions in 3D, the femtosecond primary vanadium-vanadium bond dilation, the displacements of atoms in picoseconds, and the sound wave shear motion on hundreds of picoseconds were resolved, elucidating the nature of the structural pathways and the nonconcerted mechanism of the transformation.  相似文献   

Electrons in finite-sized water cavities: hydration dynamics observed in real time     

Paik DH  Lee IR  Yang DS  Baskin JS  Zewail AH 《Science (New York, N.Y.)》2004,306(5696):672-675

We directly observed the hydration dynamics of an excess electron in the finite-sized water clusters of (H2O)n- with n = 15, 20, 25, 30, and 35. We initiated the solvent motion by exciting the hydrated electron in the cluster. By resolving the binding energy of the excess electron in real time with femtosecond resolution, we captured the ultrafast dynamics of the electron in the presolvated ("wet") and hydrated states and obtained, as a function of cluster size, the subsequent relaxation times. The solvation time (300 femtoseconds) after the internal conversion [140 femtoseconds for (H2O)35-] was similar to that of bulk water, indicating the dominant role of the local water structure in the dynamics of hydration. In contrast, the relaxation in other nuclear coordinates was on a much longer time scale (2 to 10 picoseconds) and depended critically on cluster size.  相似文献   

Comparison of biological parameters between the invasive B biotype and a new defined Cv biotype of <Emphasis Type="Italic">Bemisia tabaci</Emphasis> (Hemiptera: Aleyradidae) in China     

Bao-Li Qiu  Fang Dang  Shao-Jian Li  Muhammad Z. Ahmed  Feng-Liang Jin  Shun-Xiang Ren  Andrew G. S. Cuthbertson 《Journal of pest science》2011,84(4):419-427

The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is a widely distributed and destructive agricultural pest on various host plants. The biology of two biotypes of B. tabaci: the invasive B and a new defined Cv biotype, on a range of host plants (hibiscus, laurel, poinsettia, collard, cucumber and tomato) were studied in the laboratory. Results revealed that the developmental periods of the B biotype immatures were not significantly different on the tested host plants except those between laurel and collard. The Cv biotype immatures developed significantly slower on cucumber and tomato than on the other plants. B. tabaci B biotype had the highest survivorship on collard (68.55%), and the lowest on laurel (33.24%), while the Cv biotype had the highest and lowest survivorships on laurel (61.63%) and tomato (36.74%). Host plants did not significantly affect the pre-ovipostion period regardless of biotype. The longest averaged longevity and highest fecundity of B biotype were both recorded on collard: 25.15 days and 143.0 eggs. The highest fecundity of Cv biotype was 196.49 eggs on laurel and its longest longevity was on hibiscus (19.62 days). The intrinsic rate of natural increase (r _m) of B biotype on the three vegetables were all higher than those on the three ornamentals whereas the r _m of Cv biotype on the three ornamentals were all higher that those on the three vegetables. Our research indicates that B. tabaci B and Cv biotypes have different host plant suitabilities. The three tested vegetables were more suitable for B biotype while the three tested ornamental plants were more suitable for Cv biotype. The potential mechanism for the different suitability of B and Cv biotypes on various host plants is also discussed.  相似文献   

In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes   总被引：1，自引：0，他引：1  

Momena Khatun  Mohammad Musharraf Uddin Bhuiyan  Jalal Uddin Ahmed  Aminul Haque  Mohammad Bozlur Rahman  Mohammed Shamsuddin 《Journal of veterinary science (Suw?n-si, Korea)》2011,12(1):75-82

Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle''s salt with L-glutamine and sodium bicarbonate) for 27 h at 39℃ under 5% CO₂ in humidified air. The oocytes retrieval and efficiency (mean ± SD) per prepubertal and pubertal goats were 5.2 ± 0.6 and 6.8 ± 0.6, and 77.3 ± 0.1% and 80.5 ± 0.6%, respectively. Anaphase I - telophase I stages differed significantly (7.3 ± 0.8 vs. 2.6 ± 0.2, p < 0.05) between the two groups of goats. After IVM, the percentages of metaphase II were significantly higher (66.3 vs. 60.3, p < 0.05) in pubertal goats than in their prepubertal counterparts. The percentages of normal in vitro fertilization (IVF) in Fert-Tyrode''s albumin lactate pyruvate of pubertal goat oocytes did not differ between Percoll and swim-up sperm separation methods (36.7 ± 0.9% vs. 32.7 ± 1.3%, p > 0.05). Furthermore, sperm capacitation by heparin alone or in combination with ionomycin did not lead to a significant increase in the normal fertilization rate (34.8 ± 1.7 vs. 32.2 ± 1.5%, respectively) in the oocytes of pubertal goats. In conclusion, the ovaries of pubertal black Bengal goats obtained from the slaughterhouse could be used for in vitro embryo production. However, further optimization of the IVM and IVF techniques are necessary for satisfactory in vitro embryo production.  相似文献