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261.
Spread of invading organisms 总被引:23,自引:0,他引:23
262.
Akira IGUCHI Masahiro UENO Tsuneo MAEDA Takashi MINAMI Isao HAYASHI 《Fisheries Science》2004,70(4):569-572
ABSTRACT: The purpose of the present paper was to examine the genetic population structure of Buccinum tsubai in the Japan Sea. Mitochondrial 16SrRNA gene sequence analysis was performed with specimens from various stations in the Japan Sea. Mitochondrial lineages were clearly patterned geographically in four separate areas: the Hokkaido area, the Yamagata–Toyama area, the Yamato Bank area, and the San'in area. The main distribution depth range of B. tsubai is between 200 m and 1000 m isodepths, and the horizontal distance between the 200 m and 1000 m isodepth lines represents the specific spatial scale of the habitat (SSSH). These four areas were separated either by the complete discontinuity of the SSSH area or by its narrow spatial extension. Genetic distances between the main haplotypes of each area were calculated as Jukes–Cantor distances, the value of which ranged between 0.012 and 0.017. This value seemed to be unrelated to the geographic distance. There was no tendency for clustering according to depth. In future, the morphological characters of the four lineages of B. tsubai should be compared in detail in order to elucidate significant genetic differences among them. 相似文献
263.
Kawasumi M Anzai M Takehara T Mitani T Kato H Saeki K Iritani A Matsumoto K Hosoi Y 《The Journal of reproduction and development》2007,53(3):615-622
The majority of somatic cell nuclear transferred (SCNT) embryos die before or after implantation. Many studies have focused on morphological remodeling of the donor nucleus and its associated cytoskeletal structures in the early events of nuclear transfer. However, little is known about the 2-cell stage of SCNT embryos after the first division. In this study, we compared the morphological status of chromosomal division during the 1-cell stage to the 2-cell stage in SCNT embryos with that in intracytoplasmic sperm injection (ICSI) embryos. The microtubules and cytoplasmic asters, which are related to chromatin segregation, disappeared at the pronuclear stage, although formation of the first mitotic spindle was normal in both the SCNT and ICSI embryos. However, nuclear fragmentation was observed in 30% of the 2-cell SCNT embryos and 12% of the 2-cell ICSI embryos. Nuclear fragmentation was present in both blastomeres of these embryos. No apoptotic DNA fragmentation was observed in TdT-mediated dUTP-biotin Nick End Labeling (TUNEL) assays for either the SCNT or ICSI embryos. In both the SCNT and ICSI embryos, the distribution of chromosomes in the first mitotic spindle was disturbed during the process of division from the 1-cell stage to the 2-cell stage. These results suggest that loss of SCNT embryos just before or after implantation may be due to an abnormal chromosome distribution at the 2-cell stage. 相似文献
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Nao TSUZUKI Yoshiro ENDO Lisa KIKKAWA Kenji KOROSUE Yasuyuki KANEKO Akira KITAUCHI Hiromu KATAMOTO Yuichi HIDAKA Mitsuyoshi HAGIO Shidow TORISU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1647-1650
The performance of horses undergoing regular intense exercise is adversely affected by
oxidative stress. Thus, it is important to increase antioxidant production in horses in
order to reduce oxidative stress. Ozonated autohemotherapy (OAHT) reportedly promotes
antioxidant production. This study aimed to evaluate the effects of OAHT on antioxidant
capacity. Ten Thoroughbred horses were used in this study. After the OAHT, we collected
serum samples and measured biological antioxidant potential (BAP). We found that BAP began
to increase after the OAHT and was significantly higher in the OAHT group than at 3
(P<0.01) and 7 days (P<0.05) after OAHT than in
the control group at 3 and 7 days after starting collection of blood samples. Therefore,
it was shown that OAHT improved the antioxidant capacity of the horses. 相似文献
266.
Noriko SHIOMI-SUGAYA Kouji KOMATSU Jingwen WANG Mamoru YAMASHITA Fumitaka KIKKAWA Akira IWASE 《The Journal of reproduction and development》2015,61(3):161-168
Ovaries contain follicles at various stages of development, including primordial, primary, secondary, antral and Graafian follicles. Although the growth of these follicles is controlled to maintain regular ovulation, the mechanism through which this occurs remains unclear. In our study, we found that the growth rate of cultured secondary follicles separated from mice ovaries differed between follicles. After 4 days of culture, the size of some secondary follicles was markedly increased, while that of others had either slightly increased, remained unchanged or shrunk. We compared the expression levels of growth factors between these secondary follicles and found that the growth rate of cultured secondary follicles correlated with the expression level of insulin-like growth factor 1 (Igf1) mRNA. Igf1 mRNA expression level in secondary follicles containing theca cells was higher than that in secondary follicles without theca cells, and the
granulosa cell proliferation around follicles containing theca cells was increased. Furthermore, an IGF1 inhibitor also inhibited the granulosa cell proliferation, and administration of IGF1 to secondary follicles without growth promoted granulosa cell proliferation. These results indicated that the theca cells of secondary follicles induced the expression of IGF1 and promoted the follicle growth. 相似文献
267.
Jiratthiya TRISOMBOON ChunMei LI Akira SUZUKI Gen WATANABE Kazuyoshi TAYA 《The Journal of reproduction and development》2015,61(2):134-137
To investigate the effect of endocrine disruption of 4-nitro-3-phenylphenol (PNMPP) on immature male Wistar-Imamichi rats, the rat pituitary was exposed to PNMPP (10–5–10–9 M) for 24 h with or without gonadotropin-releasing hormone (GnRH) in experiment I. In addition, the Leydig cells (10–5–10–9 M) were exposed to PNMPP for 24 h with or without human chronic gonadotropin (hCG) in experiment II. Our results showed that the PNMPP at 10–5–10–7 M suppressed follicle-stimulating hormone (FSH) and luteinizing hormone (LH) productions from GnRH-stimulated pituitary cells. At the same time, PNMPP 10–5–10–7 M induced an increase in testosterone production from the Leydig cells treated with or without hCG. Based on our results, it can be concluded that that PNMPP might have both androgen agonist action by decreasing FSH and LH production in the pituitary and anti-androgenic action by increasing
testosterone production in the Leydig cell. 相似文献
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270.
Junta Yanai Nanami Inoue Atsushi Nakao Masahiro Kasuya Kaori Ando Toshiya Oga Takayuki Takayama Hiroyuki Hasukawa Kunihiko Takehisa Akira Takamoto Kazuki Togami Tomoki Takahashi 《Soil Use and Management》2023,39(2):785-793
To prove the hypothesis that paddy rice utilizes soil nonexchangeable potassium (neK) and causes associated structural changes in clay minerals, K status and clay mineralogy of 22 surface soils from three paddy fields under long-term fertilizer management for 51–93 years were investigated. Soil neK content was determined as the difference between 1 mol L−1 hot HNO3 extractable K and 1 mol L−1 ammonium acetate exchangeable K. Clay mineralogy was identified by X-ray diffraction (XRD). The radiocesium interception potential (RIP), an index of frayed edge sites in the interlayer sites of 2:1 type clay minerals, was also determined. The neK contents under the -K and NPK treatments were considerably lower than those under the unfertilized treatment in all the fields, indicating the exploitation of soil neK by rice. XRD analysis of the clay samples revealed 7% shift from the 1.0 peak to 1.4 nm one under the -K treatment compared with the unfertilized one, and the amounts of neK were negatively correlated with those of RIP (p < .01), suggesting the expansion of interlayer spaces of the 2:1 type phyllosilicates such as mica due to the release of neK. In addition, the neK content positively correlated with K balance of the long-term experiments (p < .05). The differences of neK between unfertilized K and -K treatments corresponded to 22–157 kg K ha−1, or 0.42–1.68 kg K ha−1 year−1. In conclusion, utilization of considerable amount of soil neK under K depleted conditions should be considered to establish sustainable K management for paddy rice. 相似文献