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431.

•Introduction  

The agricultural landscape in Eastern Canada has drastically changed in the last decades. Whilst certain forest communities are disappearing, post-agricultural forests are emerging from old fields colonized by fast-growing tree species.  相似文献   
432.
BACKGROUND: Symphylids (Hanseniella sp.) are polyphagous soilborne parasites. Today, symphylid populations on pineapple are monitored by observing root symptoms and the presence of symphylids at the bottom of basal leaves. The authors developed a reliable method with a bait and trap device to monitor symphylid populations in pineapple or fallow crops. The spatial distribution of the symphylid populations was evaluated using the variance/mean ratios and spatial analyses based on Moran's and Geary's indices. The method has been tested to monitor symphylid populations at different developmental stages of pineapple. RESULTS: Adding potato baits to the soil samples increased the trapping efficiency of symphylids when compared with ‘soil only’ and ‘bait only’ methods. The handling of the samples is also facilitated by the new device. Results showed that the vertical distribution of symphylids may be uniform deeply inside the soil profile under pineapple, up to 50 cm. Results showed that symphylid populations are highly aggregated, showing a spot area about 4–6 m wide for their development. CONCLUSION: The new method allows better and easier evaluation of symphylid populations. It may be very useful in the evaluation of new IPM methods to control symphylids under pineapple. Copyright © 2011 Society of Chemical Industry  相似文献   
433.
Inactivated virus vaccines have been widely used to control bluetongue after introduction of serotype 8 of the bluetongue virus (BTV) in northern Europe in 2006. To evaluate vaccination, quantitative knowledge of its possible side effects is needed. One current adverse reaction with inactivated vaccines is a rise in body temperature, which could reduce cow reproductive performance. The objective of this study was to quantify a possible side effect of vaccination on fertility before the implantation of the embryo of dairy cows under field conditions. The study was performed on herds that were not exposed to BTV. Fertility was assessed by return-to-service following artificial insemination (AI). Biological assumptions for a possible side effect of vaccination were conception failure and embryonic death. Associations between return-to-service rates and vaccine injections were assessed using mixed-logistic regression models and survival analysis. Two models were considered: a 3-week-return-to-service model comparing cows vaccinated between 3 days before and 16 days after AI and unvaccinated cows (assuming an effect on conception failure or early embryonic death), and a 90-day-return-to-service model comparing cows vaccinated between 3 days before and 42 days after AI and unvaccinated cows (assuming an effect on conception failure, early or late embryonic death). Only cows receiving a second vaccine injection between 2 and 7 days after AI had a significantly higher risk of 3-week-return-to-service (RR=1.19 [1.07-1.33]). This corresponds to an increase of return-to-service by 4 percentage points. A side effect of vaccination could be due to early embryonic death. The slight side effect on fertility associated with vaccination was low compared to effects of BTV-8 exposure on fertility.  相似文献   
434.
435.
ABSTRACT: Babesia divergens is a tick-transmitted apicomplexan parasite for which asexual multiplication in its vertebrate hosts is restricted to erythrocytes. Current knowledge of invasion of these target cells is limited. An efficient in vitro invasion assay was set up to gain access to this information. Parasites prepared from infected RBC, lysed by electroporation, and mixed with bovine RBC in a selected synthetic medium (RPMI 1640 supplemented with calcium) were able to establish subsequent cultures with parasitemia ranging from 6 to 14%. Free parasites remaining in the invasion medium could be eliminated by Percoll gradient and culture could be pursued with the freshly invaded erythrocytes. In this way, the invasion time window could be shortened to obtain a synchronised start of the culture or to study the kinetics of invasion. With this assay we demonstrate that 1) erythrocyte invasion by B. divergens is a rapid process since 70% of the invasion-competent parasites invaded the RBC in less than 45 s; 2) all invasion-competent parasites achieved invasion within 10 min of contact; 3) one erythrocyte could be invaded concomitantly by two merozoites; 4) despite a synchronous start, the parasite population evolved heterogeneously resulting in a progressive loss of synchronisation. Western blot analysis of proteins collected from invasion medium were performed with sera from animals experimentally infected with B. divergens and highlighted several proteins. The dose-dependent, inhibitory effects of these sera on B. divergens invasion suggest that these proteins might be involved in the invasion process. Further investigations are required for their characterisation.  相似文献   
436.
Three Allanblackia species (A. floribunda, A. stuhlmannii and A. parviflora) with high nutritive, medicinal, cosmetic and economic values are currently being domesticated as new oil tree crops. Allanblackia seeds contain a hard white fat consisting mostly of stearic (52–58%) and oleic (39–49%) acids. This unusual fatty acid content has the right properties for many different food and cosmetic products making them commercially interesting. Vegetative propagation studies on A. floribunda, which grows naturally in the moist forest of Cameroon and Nigeria, were initiated aimed at evaluating its amenability to grafting. Scions were grafted onto 18 month old rootstocks of A. floribunda using side tongue, top cleft, side veneer, whip-and-tongue methods under nursery conditions in Cameroon. In parallel, side tongue and inverted ‘T’ budding methods were also tested in situ on young A. floribunda wildings growing under semi-deciduous and evergreen tree covers. In addition, the effects of protecting side tongue new grafts with non perforated translucent plastic, perforated translucent plastic and aluminium foil were assessed. Under nursery conditions, side tongue grafts were significantly more successful (80.0 ± 6.3%), than grafts of side veneer (52.5 ± 7.9%), top cleft (55.0 ± 7.9%) and whip and tongue (50.0 ± 7.9%). The success of side tongue graft was further increased (86.7 ± 6.2%) under the shade of evergreen trees when protected by non perforated translucent plastic. These results indicate the potential for in situ grafting and ‘top working’ to promote cultivation of more productive germplasm of Allanblackia within multifunctional agricultural systems.  相似文献   
437.
Published studies on the use of stereotactic radiotherapy for dogs with pituitary tumors are limited. This retrospective observational study describes results of stereotactic radiotherapy for 45 dogs with imaging‐diagnosed pituitary tumors. All dogs were treated at a single hospital during the period of December 2009–2015. The stereotactic radiotherapy was delivered in one 15 Gray (Gy) fraction or in three 8 Gy fractions. At the time of analysis, 41 dogs were deceased. Four were alive and censored from all survival analyses; one dog received 8 Gy every other day and was removed from protocol analyses. The median overall survival from first treatment was 311 days (95% confidence interval 226–410 days [range 1–2134 days]). Thirty‐two dogs received 15 Gy (median overall survival 311 days; 95% confidence interval [range 221–427 days]), and 12 received 24 Gy on three consecutive days (median overall survival 245 days, 95% confidence interval [range 2–626 days]). Twenty‐nine dogs had hyperadrenocorticism (median overall survival 245 days), while 16 had nonfunctional masses (median overall survival 626 days). Clinical improvement was reported in 37/45 cases. Presumptive signs of acute adverse effects within 4 months of stereotactic radiotherapy were noted in 10/45, and most had improvement spontaneously or with steroids. Late effects versus tumor progression were not discernable, but posttreatment blindness (2), hypernatremia (2), and progressive neurological signs (31) were reported. There was no statistical difference in median overall survival for different protocols. Patients with nonfunctional masses had longer median overall survival than those with hyperadrenocorticism (P = 0.0003). Survival outcomes with stereotactic radiotherapy were shorter than those previously reported with definitive radiation, especially for dogs with hyperadrenocorticism.  相似文献   
438.

Objective

To assess the degree and duration of corneal anaesthesia provided by topical application of a non-ophthalmic 2% lidocaine gel in horses.

Study design

Experimental, ‘blinded’, randomized prospective study.

Animals

Twelve adult horses without relevant ocular abnormalities.

Methods

Baseline corneal touch threshold (CTT) measurements were obtained bilaterally by use of a Cochet–Bonnet aesthesiometer just prior to topical treatment. A volume of 0.2 mL of 2% lidocaine gel was administered in one randomly selected eye and the same volume of a viscous lubricant in the other eye to serve as control. The CTT value was measured on both eyes 5, 10, 20, 30, 45, 60, 75 and 90 minutes after drug application. The potential for local adverse effects following lidocaine gel application was also evaluated.

Results

Mean CTT baseline measurements were not significantly different (p > 0.05) between the control eyes (3.41 ± 0.56 cm) and those subsequently treated with the lidocaine gel (3.50 ± 0.64 cm). In control eyes, no significant changes in corneal sensitivity (p > 0.05) occurred over time during the study period. By contrast, a marked reduction in corneal sensitivity was observed after lidocaine application, with mean CTT values significantly lower (p < 0.001) than those of the control eyes from 5 to 75 minutes. A steady-state maximal corneal anaesthesia was present from 10 to 45 minutes after lidocaine gel application with mean CTT values ranging from 0.21 to 0.45 cm. Corneal epithelial irregularities were detected in three lidocaine-treated eyes, but spontaneous resolution occurred within 24 hours.

Conclusions and clinical relevance

Deep and sustained corneal anaesthesia is achieved after application of 2% lidocaine gel to the equine eye, with minimal changes in the corneal epithelium. It might be useful for minor ophthalmic surgeries performed in the standing sedated horse.  相似文献   
439.
Abstract

Phosphorus deficiency in soil and erratic rainfall limit rice production in Northeast Thailand. The objective of this study was to investigate rice's response to different rates and times of phosphorus fertilizer. A field trial was conducted in a farmer's field with a split plot design. Main plots were established using three rates: 15, 30, and 60 kg P ha?1. Five types of sub-plot were established as follows: (1) P fertilizer was applied by banding below the seeds' depth before seeding (BBS), (2) broadcasting and incorporation into the soil before seeding, and (3) topdressing at three different DAS (days after seeding). Fertilizer was spread onto the soil surface at 10, 20, and 30 DAS. Significant grain-yield increases were affected by time or rate of fertilizer application. Grain yields were maximized by a rate of 60 kg P ha?1. Fertilizer application by BBS produced higher grain yield than did other treatments, giving higher root-length density and shoot growth, phosphorus uptake, and greater phosphorus-use efficiency than did fertilizer applied by broadcasting or delayed topdressing. BBS resulted in the highest phosphorus enrichment in soil volume with a rate of 60 kg P ha?1. The high solubility of fertilizer in a localized placement may satisfy the seedlings' high initial demand of phosphorus and provide sufficient phosphorus availability to allow high productivity. Based on these data, in regions with low soil phosphorus availability where rainfall is also erratic, the management of sub-seed banding placement at the start of a crop at the rate of 60 kg P ha?1 is recommended as the optimum phosphorus fertilizer practice.  相似文献   
440.
Medium-density fibreboard (MDF) was produced from fibres treated with maleated polypropylene wax. The objectives of this study were to improve the dimensional stability of MDF panels by this treatment; to observe the maleated polypropylene wax distribution within the MDF panels using conventional fluorescence microscopy; and to determine the effects of the treatment on the mechanical properties and vertical density profile of the panels. MDF panels were produced from two resin types (urea-formaldehyde and melamine-urea-formaldehyde) and three maleated polypropylene wax contents (0, 3 and 5%). Photomicrographs show that maleated polypropylene wax forms agglomerates within the MDF panels which is an evidence of its poor distribution in our experimental conditions. Our results show an important reduction on thickness swelling and water absorption after water soaking for panels produced from treated fibres. Linear expansion and contraction in adsorption and desorption conditions between 80 and 50% relative humidity increased following fibre treatment. However, thickness swelling and shrinkage in similar conditions showed an important reduction following fibre treatment. The fibre treatment did not have negative effects on the mechanical properties or the vertical density profile of MDF panels. The modulus of rupture and modulus of elasticity in bending were increased by the treatment independently of maleated polypropylene wax content. The internal bond strength increased following the addition of 5% maleated polypropylene wax content.  相似文献   
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