Isolation of Brucella suis biovar 3 from horses in Croatia     

Cvetnic Z  Spicic S  Curic S  Jukic B  Lojkic M  Albert D  Thiébaud M  Garin-Bastuji B 《The Veterinary record》2005,156(18):584-585

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Therapy of ocular and visceral leishmaniasis in a cat     

Leiva M  Lloret A  Peña T  Roura X 《Veterinary ophthalmology》2005,8(1):71-75

An 8-year-old, spayed female Domestic Short-haired cat was referred for further evaluation of chronic lymphocytic-plasmacytic stomatitis and bilateral ocular disease. The cat had been treated with systemic glucocorticoids for several months. Initial ophthalmic examination revealed bilateral deep stromal corneal ulcers, exudative panuveitis and secondary glaucoma. Mature mild neutrophilia and monocytosis were detected on complete blood cell count. Abnormalities in the serum profile were hyperglycemia, mild azotemia, hyperglobulinemia and moderate polyclonal gammapathy. Urinalysis revealed glucosuria without ketonuria. Diabetes mellitus was diagnosed and treatment with long-acting insulin was started. An enzyme-linked immunosorbent assay was highly positive for leishmaniasis, and treatment with allopurinol was started. Although specific topical treatment was applied, melting ulcers progressed to corneal perforation and both eyes were enucleated. Ocular histology showed large numbers of intracellular organisms compatible with amastigotes of the genus Leishmania located in the uveal tract, cornea, sclera and retina. Results of inmunohistochemistry staining on ocular samples were positive for Leishmania. Bone marrow cytology demonstrated numerous macrophages with intracytoplasmatic Leishmania. Polymerase chain reaction results on bone marrow for Leishmania were positive. Three weeks later, hypoglycemic episodes permitted withdrawal of the insulin therapy. To the authors' knowledge this is the first case of ocular and visceral leishmaniasis diagnosed in vivo and under systemic treatment in a cat.  相似文献   

Structure—activity relationships of fungicidal acetylenic sulphones     

Hendrik A. Selling  Albert Tempel 《Pest management science》1976,7(1):19-29

Regression analysis in the l-alkylsulphonyl-2-arylacetylene series yielded significant correlations of antifungal activity against six microorganisms with certain physicochemical parameters (electronic effects, lipophilicity and steric factors). Fungicidal activity is enhanced by introducing electron-withdrawing substituents in the aryl nucleus, whereas variation of the lipophilic parameter reveals an optimum value. The results obtained indicate a mechanism of action based on a reaction of the compounds with nucleophiles present in the fungal cells, which was confirmed by determining the second-order rate constants of the reaction between 1 -alkylsulphonyl-2-arylacetylenes and the model nucleophile 4-nitro(thiophenol). The lipophilic para-meter was also determined experimentally using thin-layer chromatography. Regression analysis with this completely experimental set of parameters regression gave highly significant results in close agreement with those obtained above.  相似文献   

Evaluating infiltration for border irrigation models     

Albert J. Clemmens 《Agricultural Water Management》1982,5(2):159-170

The infiltration characteristics of a soil are important to the design, evaluation and management of border irrigation systems. The use and verification of border irrigation models also rely heavily on infiltration. This paper presents a technique for determining infiltration when detailed information is available on the total infiltrated volume during the irrigation which can be obtained from measurements of inflow, outflow, and water depths on the border strip. The method uses a volume balance at progressive times and is an extension of earlier work. Data from this method were used as input to the zone-inertia border irrigation model and good agreement was found between measured and computed values of advance, recession, runoff rates and volumes, and surface water depths.  相似文献   

Practical aspects at drying-off     

Sampimon OC  Vernooij JC  Sol J 《Tijdschrift voor diergeneeskunde》2004,129(24):828-833

Drying off cows with antibiotics is an effective method to cure existing infections at drying off and to prevent new infections during the dry period. In the recent years, a shift has been observed from contagious to environmental pathogens. This influences the general preventive measures and the choice for the drying off treatment. Important factors influencing the efficacy of dry cow products are general hygienic measures, length of the dry period, production at drying-off, somatic cell count at drying-off and the udder pathogens circulating on the farm.  相似文献   

Monoclonal antibody analysis of porcine reproductive and respiratory syndrome virus epitopes associated with antibody-dependent enhancement and neutralization of virus infection   总被引：14，自引：0，他引：14  

Cancel-Tirado SM  Evans RB  Yoon KJ 《Veterinary immunology and immunopathology》2004,102(3):249-262

Enhanced infection and replication of porcine reproductive and respiratory syndrome (PRRS) virus in the presence of specific antibody has been demonstrated in vitro and in vivo, a phenomenon known as antibody-dependent enhancement (ADE). ADE is considered to be a significant obstacle to developing effective vaccines for many viruses for which ADE has been reported, since virus-specific antibodies of maternal origin or those conferred by vaccination can facilitate the entry of the virus into target cells, sometimes resulting in increased severity of the disease. In this study, the role of specific PRRS viral epitopes in ADE and/or virus neutralization (VN) was assessed in vitro using 14 monoclonal antibodies (mAbs) to 4 PRRS viral proteins: nucleocapsid (N), matrix (M), glycoprotein (GP) 5, and GP3. Each mAb recognized a distinct epitope on one of these proteins. One-way ADE and VN assays were performed in vitro using homologous PRRS virus isolates in the presence or absence of each mAb. ADE activity was determined by detecting a significant increase of progeny virus yield in porcine alveolar macrophage cultures in the presence of individual mAbs. Neutralizing activity was determined by detecting a significant reduction or complete blocking of virus replication in MARC-145 cells in the presence of individual mAbs. mAbs could be categorized into 3 groups: enhancing, neutralizing and neither. Viral epitopes which are capable of inducing neutralizing antibodies appeared to reside on the M, GP3 and GP5 proteins, while epitopes that may induce ADE-mediating antibody were associated with the N and GP5 proteins. Identification of the viral proteins and antigens and epitopes responsible for ADE- and VN-mediating antibodies may provide the basis for developing efficacious second-generation vaccines for the control of PRRS virus; yet, further epitope mapping remains to be done.  相似文献   

Massive-star supernovae as major dust factories     

Sugerman BE  Ercolano B  Barlow MJ  Tielens AG  Clayton GC  Zijlstra AA  Meixner M  Speck A  Gledhill TM  Panagia N  Cohen M  Gordon KD  Meyer M  Fabbri J  Bowey JE  Welch DL  Regan MW  Kennicutt RC 《Science (New York, N.Y.)》2006,313(5784):196-200

We present late-time optical and mid-infrared observations of the Type II supernova 2003gd in the galaxy NGC 628. Mid-infrared excesses consistent with cooling dust in the ejecta are observed 499 to 678 days after outburst and are accompanied by increasing optical extinction and growing asymmetries in the emission-line profiles. Radiative-transfer models show that up to 0.02 solar masses of dust has formed within the ejecta, beginning as early as 250 days after outburst. These observations show that dust formation in supernova ejecta can be efficient and that massive-star supernovae could have been major dust producers throughout the history of the universe.  相似文献   

Evaluation of the health of free-ranging greater rheas (Rhea americana) in Argentina     

Uhart M  Aprile G  Beldomenico P  Solís G  Marull C  Beade M  Carminati A  Moreno D 《The Veterinary record》2006,158(9):297-303

The health of 22 free-ranging adult rheas (Rhea americana) examined and sampled during a translocation/reintroduction project and six juvenile rheas kept in semicaptivity was investigated, and details of their haematology and plasma biochemistry are presented. Serological testing for antibodies to infectious agents was negative for infectious laryngotracheitis, avian adenovirus, avian influenza, avian reovirus, infectious bursal disease, infectious bronchitis virus, paramyxovirus types 1, 2, and 3, fowlpox and Salmonella Pullorum. Antibodies to Chlamydophila species were found in 25 of 27 of the birds, and 22 of 25 had antibodies to Aspergillus species. Ova of gastrointestinal nematodes of the genus Capillaria were identified, and the anoplocephalid cestode Monoecocestus cf rheiphilus was identified in R americana for the first time.  相似文献   

The N-acetyl-D-glucosamine specific adhesin from Mannheimia haemolytica activates bovine neutrophils oxidative burst     

De la Mora A  Trigo F  Jaramillo L  Garfias Y  Solórzano C  Agundis C  Pereyra A  Lascurain R  Zenteno E  Suárez-Güemes F 《Veterinary immunology and immunopathology》2006,113(1-2):148-156

In this work we identified specific bovine leukocytes that were bound by the Mannheimia haemolytica adhesin molecule (MhA) and the biological effect on the leukocytes. Histochemical staining and flow cytometry showed that MhA bind neutrophils (90%) and monocytes (5%). MhA induced an oxidative response in purified neutrophils; this effect was 1.5-fold higher than the effect observed with control cells activated with Zymosan. Cellular binding by MhA was inhibited with GlcNAc and its oligomers, as well as by glycoproteins containing tri- and tetra-antennary N-glycosydically linked glycans. MhA-induced oxidative burst was significantly inhibited by GlcNAc, iodoacetamide, superoxide dismutase, and piroxicam (p<0.05). Our findings suggest that among bovine leukocytes, neutrophils are the main target for MhA, inducing production of oxidative radicals by non-opsonic mechanism that seem to play an important role in tissue damage during mannheimiosis.  相似文献   

Evaluation of dysregulation of the receptor tyrosine kinases Kit, Flt3, and Met in histiocytic sarcomas of dogs   总被引：1，自引：0，他引：1  

Zavodovskaya R  Liao AT  Jones CL  Yip B  Chien MB  Moore PF  London CA 《American journal of veterinary research》2006,67(4):633-641

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