Production and characterization of monoclonal antibodies against formalin-inactivated Nipah virus isolated from the lungs of a pig     

Imada T  Abdul Rahman MA  Kashiwazaki Y  Tanimura N  Syed Hassan S  Jamaluddin A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):81-83

Eight clones of monoclonal antibodies (Mabs) to Nipah virus (NV) were produced against formalin-inactivated NV antigens. They reacted positive by indirect immunofluorescent antibody test, and one of them also demonstrated virus neutralizing activity. They were classified into six different types based on their biological properties. These Mabs will be useful for immunodiagnosis of NV infections in animals and further research studies involving the genomes and proteins of NV.  相似文献   

The N-acetyl-D-glucosamine specific adhesin from Mannheimia haemolytica activates bovine neutrophils oxidative burst     

De la Mora A  Trigo F  Jaramillo L  Garfias Y  Solórzano C  Agundis C  Pereyra A  Lascurain R  Zenteno E  Suárez-Güemes F 《Veterinary immunology and immunopathology》2006,113(1-2):148-156

In this work we identified specific bovine leukocytes that were bound by the Mannheimia haemolytica adhesin molecule (MhA) and the biological effect on the leukocytes. Histochemical staining and flow cytometry showed that MhA bind neutrophils (90%) and monocytes (5%). MhA induced an oxidative response in purified neutrophils; this effect was 1.5-fold higher than the effect observed with control cells activated with Zymosan. Cellular binding by MhA was inhibited with GlcNAc and its oligomers, as well as by glycoproteins containing tri- and tetra-antennary N-glycosydically linked glycans. MhA-induced oxidative burst was significantly inhibited by GlcNAc, iodoacetamide, superoxide dismutase, and piroxicam (p<0.05). Our findings suggest that among bovine leukocytes, neutrophils are the main target for MhA, inducing production of oxidative radicals by non-opsonic mechanism that seem to play an important role in tissue damage during mannheimiosis.  相似文献   

AHL signals induce rubrifacine production in a bruI mutant of Brenneria rubrifaciens     

McClean AE  Duerkop BA  Greenberg EP  Kluepfel DA 《Phytopathology》2012,102(2):195-203

Several members of the bacterial genus Brenneria are pathogenic on different tree species. Cell-free extracts from the bacterial phytopathogens Brenneria rubrifaciens, B. salicis, and B. nigrifluens induced production of the red pigment rubrifacine in the B. rubrifaciens bruI insertional mutant Br-212. Analysis of the bruI locus identified an adjacent open reading frame, designated bruR, with homology to luxR. High-performance liquid chromatography and mass spectrometry analysis of ethyl acetate extracts from wild-type B. rubrifaciens and Escherichia coli expressing the bruI gene identified two acyl homoserine lactone (AHL) peaks, N-(3-oxohexanoyl)-homoserine lactone (3OC6HSL) and N-hexanoyl-homoserine lactone (C6HSL). Addition of synthetic 3OC6HSL and C6HSL at 10 μM to the bruI mutant, strain Br-212, induced rubrifacine production and the ability to elicit a hypersensitive reaction (HR) in tobacco leaves. Synthetic C6HSL was less effective at inducing pigment production than 3OC6HSL at 10 μM. The bruI mutant Br-212 did not produce detectable AHLs, indicating that C6HSL and 3OC6HSL are the major AHLs produced by this species. The AHLs N-heptanoyl-DL-homoserine lactone (C7HSL), N-octanoyl-DL-homoserine lactone (C8HSL), and N-(3-oxooctanoyl)-DL-homoserine lactone (3OC8HSL) also induced pigment production in Br-212 and restored its ability to elicit an HR in tobacco, suggesting that cross-talk with other bacterial species may be possible.  相似文献   

Fitness cost, cross resistance and realized heritability of resistance to imidacloprid in Spodoptera litura (Lepidoptera: Noctuidae)     

Naeem AbbasSarfraz Ali Shad  Muhammad Razaq 《Pesticide biochemistry and physiology》2012,103(3):181-188

Imidacloprid has been used as a key insecticide for controlling sucking insect pests of cotton, whereas Spodoptera litura also has been indirectly exposed to this insecticide in Pakistan. To evaluate the risk of resistance evolution and to develop a better resistance management strategy, a field collected population was selected with imidacloprid in the laboratory. Thereafter, fitness cost, realized heritability and cross resistance of imidacloprid resistance in S. litura were investigated. After 14 generations of selection with imidacloprid, S. litura developed a 137.48-fold resistance to the insecticide. Bioassay revealed that this strain showed cross-resistance to acetamiprid (RR 8.52) and a little to lamdacyhalothrin (1.92) but negative cross-resistance was found to methomyl (−0.19). The resistant strain had a relative fitness of 0.38, with substantially lower rates of larval survival, larval duration, male pupal duration, development time, emergence rate of healthy adults, fecundity, hatchability, and prolonged larval and pupal duration. Mean relative growth rate of the larvae, intrinsic rate of population increase, and biotic potential was lower for the selected populations. The estimated realized heritability (h²) of imidacloprid resistance was 0.15 in the resistant strain of S. litura. Development of the resistance may cost significant fitness for the resistant population. This study provided valuable information for further understanding the impact of imidacloprid resistance on physiological parameters of S. litura and for facilitating the development of resistance management strategies.  相似文献   

塔克拉玛干沙漠荒漠过渡带空气动力学粗糙度分析     

杨兴华  艾力·买买提明  张瑞军  何清  霍文 《干旱区研究》2012,29(3):524-528

根据塔克拉玛干沙漠北缘荒漠过渡带哈德、肖塘2个试验点的风、温梯度观测数据,利用风速比法计算了中性大气层结、无风沙运动条件的空气动力学粗糙度[WTBX](z0),并分析探讨z0[WTBZ]与下垫面状况、大气稳定度及风速的相互关系。结果表明：哈德、肖塘中性条件下z₀的取值范围分别为 1.81×10^-11～1.20×10^-3m,1.00×10^-11～1.65×10^-3m,平均值为2.70×10^-5m 和6.05×10^-5m,与平坦沙面的值较为接近;z₀随下垫面性质变化明显;空气动力学粗糙度总体上随着理查逊数(R_i)增大而变大,但又呈现出一定的离散程度;空气动力学粗糙度与2 m高度风速呈显著的负指数关系;大气稳定度对空气动力学粗糙度的影响作用有待于进一步确定。   相似文献   

Mapping of tms8 gene for temperature‐sensitive genic male sterility (TGMS) in rice (Oryza sativa L.)     

Appibhai J. Hussain  Jauhar Ali  Ebrahimali A. Siddiq  Vidya S. Gupta  Umesh K. Reddy  Prabhakar K. Ranjekar 《Plant Breeding》2012,131(1):42-47

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Ultrasonography of the liver and kidneys of healthy camels (Camelus dromedarius)     

Mohamed Tharwat  Fahd Al-Sobayil  Ahmed Ali  Sébastien Buczinski 《The Canadian veterinary journal. La revue veterinaire canadienne》2012,53(12):1273-1278

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Characterization of turkey inducible nitric oxide synthase and identification of its expression in the intestinal epithelium following astrovirus infection     

Meyerhoff RR  Nighot PK  Ali RA  Blikslager AT  Koci MD 《Comparative immunology, microbiology and infectious diseases》2012,35(1):63-69

The inducible nitric oxide synthase (iNOS) enzyme has long been recognized as a key mediator of innate immune responses to infectious diseases across the phyla. Its role in killing or inactivating bacterial, parasitic, and viral pathogens has been documented in numerous host systems. iNOS, and its innate immune mediator NO has also been described to have negative consequence on host tissues as well; therefore understanding the pathogenesis of any infectious agent which induces iNOS expression requires a better understanding of the role iNOS and NO play in that disease. Previous studies in our laboratory and others have demonstrated evidence for increased levels of iNOS and activity of its innate immune mediator NO in the intestine of turkeys infected with astrovirus. To begin to characterize the role iNOS plays in the innate immune response to astrovirus infection, we identified, characterized, developed tkiNOS specific reagents, and demonstrated that the intestinal epithelial cells induce expression of iNOS following astrovirus infection. These data are the first to our knowledge to describe the tkiNOS gene, and demonstrate that astrovirus infection induces intestinal epithelial cells to express iNOS, suggesting these cells play a key role in the antiviral response to enteric infections.  相似文献   

Comparative Evaluation of Conventional and Transvaginal Laparoscopic Ovariohysterectomy in Dogs     

Jalal Bakhtiari DVM  PhD  Ali Reza Khalaj MD  Elham Aminlou DVM  Amir Niasari‐Naslaji DVM  PhD 《Veterinary surgery : VS》2012,41(6):755-758

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The pharmacokinetics and metabolism of meloxicam in camels after intravenous administration     

Wasfi IA  Al Ali WA  Agha BA  Kamel AM  Al Biriki NA  Al Neaimi KM 《Journal of veterinary pharmacology and therapeutics》2012,35(2):155-162

The pharmacokinetics and metabolism of meloxicam was studied in camels (Camelus dromedarus) (n = 6) following intravenous (i.v.) administration of a dose of 0.6 mg·kg/body weight. The results obtained (mean ± SD) were as follows: the terminal elimination half-life (t(1/2β) ) was 40.2 ± 16.8 h and total body clearance (Cl(T) ) was 1.94 ± 0.66 mL·kg/h. The volume of distribution at steady state (V(SS)) was 92.8 ± 13.7 mL/kg. One metabolite of meloxicam was tentatively identified as methylhydroxy meloxicam. Meloxicam and metabolite were excreted unconjugated in urine. Meloxicam could be detected in plasma 10 days following i.v. administration in camels using a sensitive liquid chromatography tandem mass spectrometry (LC/MS/MS) method.  相似文献