Predicting percentage of intramuscular fat using two types of real-time ultrasound equipment     

Hassen A  Wilson DE  Amin VR  Rouse GH  Hays CL 《Journal of animal science》2001,79(1):11-18

In the present study, 500 steers were used to develop models for predicting the percentage of intramuscular fat (PIMF) in live beef cattle. Before slaughter, steers were scanned across the 11th and 13th ribs using Aloka 500V (AL-500) and Classic Scanner 200 (CS-200) machines. Four to five images were collected per individual steer using each machine. After slaughter, a cross-sectional slice of the longissimus muscle from the 12th rib facing was used for chemical extraction to determine actual carcass percentage of intramuscular fat (CPIMF). Texture analysis software was used by two interpreters to select a region for determination of image parameters, which included Fourier, gradient, histogram, and co-occurrence parameters. Four prediction models were developed separately for each of AL-500 and CS-200 based on images captured by the respective machines. These included models developed without transformation of CPIMF (Model I), models based on logarithmic transformation of CPIMF (Model II), ridge regression procedure (Model III), and principal component regression procedure (Model IV). Model R2 and root mean square error of AL-500 Models I, II, III, and IV were 0.72, 0.84%; 0.72, 0.85%; 0.69, 0.91%; and 0.71, 0.86%; respectively. The corresponding R2 and root mean square error values of CS-200 Models I, II, III, and IV were 0.68, 0.87%; 0.70, 0.85%; 0.64, 0.94%; and 0.65, 0.91%; respectively. Initially, AL-500 and CS-200 prediction models were validated separately on an independent data set from 71 feedlot steers. The overall mean bias, standard error of prediction, and rank correlation coefficient across the four AL-500 models were 0.42%, 0.84%, and 0.88, respectively. For the four CS-200 models, the corresponding overall mean values were 0.67%, 0.81%, and 0.91, respectively. In a second validation test, only Model II of AL-500 and CS-200 was evaluated separately based on data from 24 feedlot steers. The overall mean bias, absolute difference, and standard error of prediction of AL-500 Model II were 0.71, 0.92, and 0.98%. For CS-200 Model II, the corresponding values were 0.59, 0.97, and 1.03%. Both AL-500 and CS-200 equipment can be used to accurately predict PIMF in live cattle. Further improvement in the accuracy of prediction equations could be achieved through increasing the development data set and the variation in PIMF of cattle used.  相似文献   

Some cardiovascular and respiratory effects of alfentanil in animals     

M Atef  S A Youssef  M A Shalaby  M G el-Sayed  W A Amin 《DTW. Deutsche tier?rztliche Wochenschrift》1987,94(6):333-336

  相似文献   

Application of touchdown enzyme time release (TETR)-PCR for diagnosis of Chlamydophila abortus infection     

Amin AS 《Research in veterinary science》2003,74(3):213-217

Chlamydophila abortus-DNA was detected using a touchdown enzyme time-release (TETR)-polymerase chain reaction (PCR) assay as an improved test for sensitive and rapid diagnosis of abortion in small ruminants. Two hundred and fifty two placentae, liver or spleen tissue samples from aborting ewes and goats or aborted lambs and kids in which C. abortus infection was suspected were examined by TETR-PCR and the results were compared with cell culture. Sixty-five tissue samples were found to be TETR-PCR positive while only 56 samples were cell culture-positive. After resolution of discrepant samples with a confirmatory nested PCR assay, TETR-PCR had a sensitivity of 97% and a specificity of 99.5% while culture had a sensitivity of 84.8% and a specificity of 100%. The analytical sensitivity of the TETR-PCR assay was determined with DNA extracted from 4-fold serial dilution of C. abortus B577 culture and found to be 0.25 inclusion-forming unit per PCR. No reduction in the analytical sensitivity was noted when the assay was tested with mouse liver samples spiked with 4-fold serial dilution of C. abortus B577 culture. No target product was amplified when DNA from Chlamydophila pecorum was tested. TETR-PCR used in this study is a practical, rapid, sensitive and specific assay that could be used for the detection of C. abortus in infected tissue samples. We recommend the use of this assay as a supplemental diagnostic tool for detection of C. abortus in infected tissue samples.  相似文献   

Detection of Brucella species in the milk of infected cattle,sheep, goats and camels by PCR     

Hamdy ME  Amin AS 《Veterinary journal (London, England : 1997)》2002,163(3):1466-305

One hundred and three milk samples were collected from 52 cows, 21 ewes, 18 goats and 12 camels. The animals tested positive to at least one of the following: (1) standard tube agglutination test (SAT); (2) Rose Bengal plate test (RBPT); (3) milk ring test (MRT). All milk samples were examined by culture and single-step polymerase chain reaction (PCR) techniques for detection of Brucella species. The PCR assay amplified Brucella-DNA from 29 bovine milk samples, 10 from sheep, 13 from goats and one from a camel. The direct culture method detected Brucella organisms from 24 samples of cows' milk, 12 from sheep, 10 from goats and failed to detect any Brucella organisms from camels' milk. PCR detected up to 100 colony forming units (CFU) of B. abortus per millilitre of milk in 100% of diluted milk samples, and 1000 CFU of B. melitensis from 70% of milk samples. Although the overall sensitivity of the PCR was higher than the culture method, it should be possible to increase the sensitivity to detect lower numbers of Brucella organisms in field samples. The speed and sensitivity of the PCR assay suggest that this technique could be useful for detection of Brucella organisms in bovine milk, as well as in sheep, goat, and camels milk.  相似文献   

Antimicrobial activity of crude methanolic extract of Satureja khuzistanica     

Amanlou M  Fazeli MR  Arvin A  Amin HG  Farsam H 《Fitoterapia》2004,75(7-8):768-770

The methanolic extract of the aerial parts of Satureja khuzistanica was investigated for its antimicrobial activity. The maximum antibacterial and antifungal activities were observed against Staphylococcus aureus and Candida albicans.  相似文献   

Species composition of Modellidae and Cerambycidae (Coleoptera) in a coppice woodland     

Amin Setyo Leksono  Kenta Takada  Nobukazu Nakagoshi  Koji Nakamura 《Journal of Forest Research》2006,11(1):61-64

A continuous sampling of canopy beetles was carried out to determine variations in the abundance, species diversity, richness, and composition of the Mordellidae and Cerambycidae in a coppice woodland. Changes in the abundance and the species richness were monitored at three heights in the forest throughout the season in 1999, using yellow and blue water pan traps. The results showed significant variations in the abundance of Mordellidae among the canopy layers, while little variation was found for Cerambycidae. The abundance, species diversity, and richness were generally greater in summer. The results showed distinct species compositions in both families among layers.  相似文献   

Ecophysiological and genetic variation in seedling traits and in first-year field performance of eight <Emphasis Type="Italic">Acacia senegal</Emphasis> provenances in the Blue Nile,Sudan     

El Amin Yousif Raddad 《New Forests》2007,34(3):207-222

The genetic variation in seed weight, seed number per kg and seedling traits was compared among eight Acacia senegal provenances originating from the clay plain (east) and sand plains (west) of the gum belt in Sudan. The main objective of this study was to identify germplasm sources of A. senegal that have a good seed germination capacity and seedling traits suitable for reforestation in the clay-soil part of the dryland gum belt in the Blue Nile region in Sudan. A specific objective was to tentatively explore the adaptive strategy of A. senegal populations. Seventeen-week-old seedlings were planted in the field at spacing of 3 m × 3 m, giving 100 trees per plot and replication; within a randomized complete block design with four replications. The experimental site was in the clay plain region. Seed variables showed significant differences. Clay plain provenances showed considerable variation in seed weight and seed number. They had the smallest seed weight but the highest seed number, while the sand (western) provenances had the largest seed weight but lowest seed number. Seedling branch number, root length, root to shoot ratio and shoot dry weight differed significantly among the provenances 12 weeks after germination. Clay provenances had the highest branch number and shoot dry weight but the shortest roots and lowest root to shoot ratio. This was interpreted as showing better adaptation to the site in these local provenances in comparison to those originating from the western sandy soil regions. High positive correlations were observed between seedling variables, such as root nodule and branch numbers; this could be used for early selection. The variation was greater between provenance groups than within them, suggesting that especially selection among groups would yield genetic gain.  相似文献   

Image analysis and bending properties of model OSB panels as a function of strand distribution,shape and size     

Takuya?NishimuraEmail author  Jaymeen?Amin  Martin?P.?Ansell 《Wood Science and Technology》2004,38(4):297-309

The technique of image analysis has been used to assess the quality of model oriented strand board panels by investigating the relationships between shape and size of strands, the distribution of strands and bending properties. A batch of commercial strands was analysed by image analysis and the distribution of the shape and size of strands was quantified. The strands were categorised into five strand types as a function of size and aspect ratio. In general, strand shapes were observed to be mostly rectangular and there was also a wide variation in strand dimensions in commercial material. Bigger area strands had low aspect ratios and small strands had high aspect ratios. Half of the commercial strands were longer than 100 mm.Model OSB panels were manufactured in the laboratory by hot pressing strand mats formed from each of the five strand types. Strands were laid up by hand into the forming mat and following pressing the orientation and shape of strands was evaluated by image analysis and the panels were tested in a three point bending. Large area (type 3) strands with high aspect ratios produced model panels with optimum strand orientation and mechanical properties.Type 3 panels were also fabricated from strands dropped through a slotted forming device in order to simulate the delivery of strands to the forming line under factory conditions. As the height of strand delivery increased from 0 to 100 to 200 mm the disorientation of strands in the pressed panels progressively increased and as a result mechanical properties in bending were reduced.Image analysis is therefore a powerful tool for evaluating the distribution of commercial strand shapes and the relationship between strand geometry, strand orientation and the mechanical properties of oriented strand board.  相似文献   

Effects of the addition of Calanopia elliptica,Artemia franciscana,and Brachionus rotundiformis in a nursery biofloc system on water quality,growth, gut morphology,health indices,and transcriptional response of immune and antioxidant-related genes in Penaeus vannamei     

Abbaszadeh  Akbar  Mozanzadeh  Mansour Torfi  Qasemi  Ahmad  Oujifard  Amin  Nafisi Bahabadi  Mahmod 《Aquaculture International》2022,30(2):653-676

Aquaculture International - A 56-day study was carried out to examine the addition of live foods a biofloc technology (BFT) system on Penaeus vannamei postlarvae performance (PL25, initial weight:...  相似文献   

Potential angiotensin I converting enzyme inhibitory peptides from gluten hydrolysate: Biochemical characterization and molecular docking study     

Ahmad Asoodeh  Leyla Haghighi  Jamashidkhan Chamani  Mohamad Amin Ansari-Ogholbeyk  Zahra Mojallal-Tabatabaei  Milad Lagzian 《Journal of Cereal Science》2014

The present study was carried out to characterize ACE inhibitory peptides which are released from the trypsin hydrolysate of wheat gluten protein. In silico proteolitic digestion of a high molecular weight glutenin subunit was performed. Among the resultant fragments, four peptides were selected for chemical synthesis based on the chemoinformatics studies and docking properties. The ACE inhibitory activity and kinetic parameters of the most important peptides were determined. Molecular docking simulation was also performed to predict the sites on ACE in which these peptides bind and displayed inhibition mechanisms. Two peptide sequences of IPALLKR (P4) and AQQLAAQLPAMCR (P6) showed higher ACE inhibitory activity among peptide collection. The IC₅₀ values of P6 and P4 were 43 ± 1.3 μM and 68 ± 2.8 μM, respectively. P6 peptide was proved to be a more potent ACE inhibitor than P4 peptide. Lineweaver-Burk plots revealed that P6 and P4 behaved as non-competitive and competitive ACE inhibitors, respectively. The simulations showed that P4 bound to the active site region. Conversely, P6 bound to the N-terminus entrance of substrate tunnel and obstructed the substrate access into the catalytic site. Overall, the results showed that these peptides would be considered as a model for discovering new bio-compatible ACE inhibitors.  相似文献