Working the crowd     

Gaggioli A  Riva G 《Science (New York, N.Y.)》2008,321(5895):1443

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Impacts of wood extraction on soil: assessing rutting and soil compaction caused by skidding and forwarding by means of traditional and innovative methods     

Marra  Elena  Laschi  Andrea  Fabiano  Fabio  Foderi  Cristiano  Neri  Francesco  Mastrolonardo  Giovanni  Nordfjell  Tomas  Marchi  Enrico 《European Journal of Forest Research》2022,141(1):71-86

European Journal of Forest Research - Intensive forestry operations may cause soil compaction, plastic soil disturbances and rutting, which are responsible for undesirable effects on soils,...  相似文献   

Extending the horizons of agricultural research and extension: Methodological challenges     

Andrea Cornwall  Irene Guijt  Alice Welbourn 《Agriculture and Human Values》1994,11(2-3):38-57

The recent enthusiasm for “participation” in agricultural development has fueled the development of new approaches to research and extension. The rhetoric of “participation” extends the horizons of agricultural research and extension beyond technical problem-solving. Yet in practice few of the personal, political, and experiential aspects of this process are addressed. This paper aims to draw attention to these elements of practice and to locate research and extension within wider social processes. Through a critique of conventional methodological strategies, this paper considers the possibilities offered by “participatory” alternatives. Considering the scope and objectives of agricultural development raises a series of methodological questions: What counts as knowledge? Who defines and represents this knowledge? Whose knowledge counts? Knowledge for what? Knowledge for whom? The paper goes on to assess a number of these “new” methodologies, within and beyond agricultural development. Through a consideration of their strengths and weaknesses, a series of further issues are highlighted for future methodological development. It is argued that for agricultural research and extension to acknowledge process, closer attention needs to be paid to context. The activities of research and extension need to be set in time. Strategies are needed to explore and address diversity and difference in communities. Situating the actors and agencies involved in development within relations of power involves addressing—and redressing—the nature of interactions between these actors. These changes require not an ever increasing array of methods, it is argued, but new approaches to learning.  相似文献   

Evaluation of the agreement between a point‐of‐care lactate meter and a handheld laboratory analyzer in cats treated in emergency practice     

Mark J. Acierno  Aubrey Hirsch  Andrea Dedeaux 《Journal of Veterinary Emergency and Critical Care》2020,30(1):50-53

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Effect of prior general anesthesia or sedation and antiseizure drugs on the diagnostic utility of wireless video electroencephalography in dogs     

Thomas Parmentier  Gabrielle Monteith  Miguel A. Cortez  Franziska Wielaender  Andrea Fischer  Tarja S. Jokinen  Hannes Lohi  Sean Sanders  Veronique Sammut  Tricia Tai  Fiona M. K. James 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(5):1967-1974

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Design of ecoregional monitoring in conservation areas of high-latitude ecosystems under contemporary climate change     

Erik A. Beever  Andrea Woodward 《Biological conservation》2011,(5):1258-1269

Land ownership in Alaska includes a mosaic of federally managed units. Within its agency’s context, each unit has its own management strategy, authority, and resources of conservation concern, many of which are migratory animals. Though some units are geographically isolated, many are nevertheless linked by paths of abiotic and biotic flows, such as rivers, air masses, flyways, and terrestrial and aquatic migration routes. Furthermore, individual land units exist within the context of a larger landscape pattern of shifting conditions, requiring managers to understand at larger spatial scales the status and trends in the synchrony and spatial concurrence of species and associated suitable habitats. Results of these changes will determine the ability of Alaska lands to continue to: provide habitat for local and migratory species; absorb species whose ranges are shifting northward; and experience mitigation or exacerbation of climate change through positive and negative atmospheric feedbacks. We discuss the geographic and statutory contexts that influence development of ecological monitoring; argue for the inclusion of significant amounts of broad-scale monitoring; discuss the importance of defining clear programmatic and monitoring objectives; and draw from lessons learned from existing long-term, broad-scale monitoring programs to apply to the specific contexts relevant to high-latitude protected areas such as those in Alaska. Such areas are distinguished by their: marked seasonality; relatively large magnitudes of contemporary change in climatic parameters; and relative inaccessibility due to broad spatial extent, very low (or zero) road density, and steep and glaciated areas. For ecological monitoring to effectively support management decisions in high-latitude areas such as Alaska, a monitoring program ideally would be structured to address the actual spatial and temporal scales of relevant processes, rather than the artificial boundaries of individual land-management units. Heuristic models provide a means by which to integrate understanding of ecosystem structure, composition, and function, in the midst of numerous ecosystem drivers.  相似文献   

Assessment of the Potential Cyto�CGenotoxicity of the Nonsteroidal Anti-Inflammatory Drug (NSAID) Diclofenac on the Zebra Mussel (Dreissena polymorpha)     

Marco Parolini  Andrea Binelli  Alfredo Provini 《Water, air, and soil pollution》2011,217(1-4):589-601

A battery of eight biomarkers was used on the freshwater bivalve Dreissena polymorpha in order to evaluate potential sub-lethal effects of the nonsteroidal anti-inflammatory drug diclofenac (DCF; 2-[(2,6-dichlorophenyl)amino]phenylacetic acid). By an in vivo approach, mussels were exposed for 96 h to increasing concentrations (0.3, 1, and 2 nM) of DCF perfectly comparable with current surface water levels. We determined the single cell gel electrophoresis assay, the apoptotic frequency (DNA Diffusion assay), the micronucleus test (MN test), and the lysosomal membrane stability (Neutral Red Retention Assay) in mussel hemocytes. Moreover, the activity of catalase, superoxide dismutase, glutathione peroxidase, and the phase II detoxifying enzyme glutathione S-transferase was measured in the cytosolic fraction extracted from a pool of entire bivalves to reveal possible alterations of the oxidative status of exposed specimens. The biomarker battery pointed out a negligible cyto- and genotoxicity on zebra mussel hemocytes since only a slight decrease of lysosomal membrane stability from baseline levels was measured at the end of exposures at the highest concentration (2 nM). In addition, environmental concentrations of DCF seem to have a negligible effect on the activities of antioxidant and detoxifying enzymes.  相似文献   

Antioxidant Activity in the Zebra Mussel (Dreissena polymorpha) in Response to Triclosan Exposure     

Andrea Binelli  Marco Parolini  Alessandra Pedriali  Alfredo Provini 《Water, air, and soil pollution》2011,217(1-4):421-430

The biocide triclosan (TCS, 5-chloro-2-(2,4-dichlorophenoxy)phenol) is commonly used in several personal care products, textiles, and children??s toys. Because the removal of TCS by wastewater treatment plants is incomplete, its environmental fate is to be discharged into freshwater ecosystems, where its ecological impact is largely unknown. The aim of this study was to determine the effect of TCS on the antioxidant enzymatic chain of the freshwater mollusk zebra mussel (Dreissena polymorpha). We measured the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as the phase II detoxifying enzyme glutathione S-transferase (GST) in zebra mussel specimens exposed to 1 nM, 2 nM, and 3 nM TCS in vivo. The mussels were exposed for 96 h, and the enzyme activities were measured every 24 h. We measured clear activation of GST alone at all three dose levels, which shows a poor induction of the antioxidant enzymatic chain by TCS. CAT and SOD were activated only at 3 nM, while GPx values overlapped the baseline levels.  相似文献   

Ventricular septal defect with aortic valve insufficiency in a New Zealand White rabbit     

Vörös K  Seehusen F  Hungerbühler S  Meyer-Lindenberg A  von der Hoeh N 《Journal of the American Animal Hospital Association》2011,47(4):e42-e49

A heart murmur was detected in a 10 mo old, female New Zealand White rabbit. Auscultation revealed cardiac murmurs both at the left and right hemithorax. Phonocardiography confirmed the systolic-diastolic nature of the left-sided and the systolic character of the right-sided murmur. Electrocardiography showed normal sinus rhythm; tall R waves and large T waves in lead II; and deep S waves in leads II, III, and aVF. Thoracic radiography demonstrated generalized cardiomegaly with prominent pulmonary vasculature. Echocardiography revealed a perimembraneous ventricular septal defect with aortic insufficiency. Signs of biventricular volume overload, relative pulmonic stenosis, and pulmonary valve insufficiency were also seen as consequences of the defect. Necropsy demonstrated a ventricular septal defect just below the aortic valve, a dilated pulmonary trunk, dilated and hypertrophied ventricles, dilated atria, and rightward displacement of the aortic root. Cardiac histopathology showed ventricular cardiomyocyte degeneration (swelling and hypereosinophilia of the cytoplasm with a loss of cross striation, and nuclear hyperchromasia), cartilaginous metaplasia of the aorta, and subendocardial fibrosis of the right ventricular flow tract.  相似文献   

Genetic complexity and multiple infections with more Parvovirus species in naturally infected cats     

Battilani M  Balboni A  Ustulin M  Giunti M  Scagliarini A  Prosperi S 《Veterinary research》2011,42(1):43

ABSTRACT: Parvoviruses of carnivores include three closely related autonomous parvoviruses: canine parvovirus (CPV), feline panleukopenia virus (FPV) and mink enteritis virus (MEV). These viruses cause a variety of serious diseases, especially in young patients, since they have a remarkable predilection for replication in rapidly dividing cells. FPV is not the only parvovirus species which infects cats; in addition to MEV, the new variants of canine parvovirus, CPV-2a, 2b and 2c have also penetrated the feline host-range, and they are able to infect and replicate in cats, causing diseases indistinguishable from feline panleukopenia. Furthermore, as cats are susceptible to both CPV-2 and FPV viruses, superinfection and co-infection with multiple parvovirus strains may occur, potentially facilitating recombination and high genetic heterogeneity. In the light of the importance of cats as a potential source of genetic diversity for parvoviruses and, since feline panleukopenia virus has re-emerged as a major cause of mortality in felines, the present study has explored the molecular characteristics of parvovirus strains circulating in cat populations. The most significant findings reported in this study were (a) the detection of mixed infection FPV/CPV with the presence of one parvovirus variant which is a true intermediate between FPV/CPV and (b) the quasispecies cloud size of one CPV sample variant 2c. In conclusion, this study provides new important results about the evolutionary dynamics of CPV infections in cats, showing that CPV has presumably started a new process of readaptation in feline hosts.  相似文献