Expression of toll-like receptor 2 (TLR2) in porcine leukocyte subsets and tissues     

Alvarez B  Revilla C  Doménech N  Pérez C  Martínez P  Alonso F  Ezquerra A  Domíguez J 《Veterinary research》2008,39(2):13

Toll-like receptors (TLR) are a group of pattern recognition molecules that play a crucial role in innate immunity. TLR2 recognises a variety of microbial components leading to the development of inflammatory and immune responses. To characterise the expression and functional properties of porcine TLR2 (pTLR2), we have raised a panel of monoclonal antibodies (mAb) against this molecule. Mouse 3T3 cell transfectants expressing pTLR2 were used for immunisation of mice. The specificity of these antibodies was confirmed by their reactivity with CHO cells transfected with pTLR2 but not with pTLR4 or with non-transfected cells. Using one of these mAbs, named 1H11, pTLR2 was found on cells of the innate immune system, including monocytes, macrophages, and granulocytes, but not on peripheral blood lymphocytes. Staining of tissue sections showed that pTLR2 is also expressed on epithelial cells lining the tracheobronchial and intestinal tracts, bile ducts in the liver and renal tubules, and on the basal layer of the epidermis. This distribution is consistent with a surveillance function at entry sites, allowing for early detection of microbial invasion.  相似文献   

Genetic variation analysis of the genus Passiflora L. using RAPD markers     

Diego Fajardo  Fernando Angel  Mikkel Grum  Joe Tohme  Mario Lobo  William M. Roca  Inés Sanchez 《Euphytica》1998,101(3):341-347

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Bottled-Water Quality in Metropolitan Mexico City     

Robles  Esperanza  Ramírez  Pedro  Elena González  Ma.  de Guadalupe Sáinz  Ma.  Martínez  Blanca  Durán  Angel  Elena Martínez  Ma. 《Water, air, and soil pollution》1999,113(1-4):217-226

Bacteriological and physico-chemical parameters of 265 samples from 39 brands sold in 5-gallon plastic and glass bottles and 2-5 L plastic containers were analyzed to determine the quality of bottled water distributed in Mexico City. Tests included fecal and total coliform counts, alkalinity, total hardness, chloride, calcium and magnesium concentrations, pH and conductivity. Correlation and cluster analyses and ANOVA were carried out, and a comparison made of the averages with the maximum permissible levels established in the Official Mexican Norms. Concerning the investigated parameters no differences (p > 0.05) between the brands were found. Physico-chemical parameters were studied and all the samples were within the permissible limits. Most samples taken from the 5-gallon containers exceeded the maximum bacteriological limits. It was concluded that the bacteriological quality of the brands studied was extremely variable. Appropriate sanitary measures, should be established to control this aspect.  相似文献   

Simple estimation of available water capacity in soils of semiarid and subhumid environments     

Josefina Luisa De Paepe  Alfredo Angel Bono  Roberto Alvarez 《Arid Land Research and Management》2018,32(2):133-148

Soil available water capacity (AWC) is an important factor affecting soil productivity in semiarid and subhumid environments and is mainly determined by the soil textural composition. As the soils of these environments usually present fairly uniform textures across depth, we hypothesized that it would be possible to accurately estimate the whole-profile AWC using surface information. Our objective was to test this hypothesis in the Argentine Semiarid Pampas. Information was collected from 152 sites where AWC was measured in 20?cm layers up to a depth of 140?cm or up to the upper limit of the petrocalcic horizon, when present. In each case, whole profile AWC was estimated using a one-step and a two-step approach, comparing multiple regression and artificial neural networks as modeling techniques. Both modeling methods were effective (R²?>?0.76, P?相似文献   

Effect of the Cooking Process on the Gelling Properties of Whole and Minced Jumbo Lump of Blue Crab (Callinectes sapidus)     

Miguel Angel Martínez-Maldonado  José Alberto Ramírez-De León  Ma. Guadalupe Méndez-Montealvo  Eduardo Morales-Sánchez 《Journal Of Aquatic Food Product Technology》2018,27(4):418-429

Commercially, crab meat is obtained by hand-picking after a cooking process, followed by pasteurizing or canning. Jumbo lump is one of the pieces of meat obtained from the blue crab. The aim of this study was to evaluate the effect of the commercial cooking process (boiling and steaming) on the mechanical and functional properties of whole jumbo lump and the gels obtained from cooked and minced jumbo lump from blue crab (Callinectes sapidus). The gels were prepared combining the washing process and microbial transglutaminase. The boiling and steaming processes showed a different effect on the textural and color properties. Whole jumbo lump pieces cooked by immersion in boiling water showed a higher hardness value as compared to steamed jumbo lump pieces, suggesting a different internal structural. The cooking processes used in this study did not modify the water holding capacity, gelling capability, or color of the minced jumbo lump. The addition of microbial transglutaminase to washed minced jumbo lump improved the hardness, cohesiveness, and chewiness of gels elaborated with boiled or steamed jumbo lump, which allowed the textural properties similar to those of the whole jumbo lump.  相似文献   

Mineral contents of the muscle tissue and the digestive gland of Octopus vulgaris during short‐term starvation          下载免费PDF全文

P. S. Morillo‐Velarde  J. Cerezo Valverde  B. García García 《Aquaculture Nutrition》2018,24(2):886-893

We used spectrometry to study variation in the contents of minor (B, Cu, Fe, Mn and Zn) and major essential elements (Ca, K, Mg, Na and P) in the muscle tissue and digestive glands of Octopus vulgaris after 1, 2, 4 or 8 days of starvation and in fed controls. The contents of the major essential elements remained constant during 8 days of starvation. Na was the principal element found in both tissues, followed by K, P and Mg. The contents of the minor essential elements were higher in the digestive gland than in the muscle tissue, with increased contents of Fe, Mn and Zn (p < .05) during starvation, confirming the high capacity of this organ to accumulate trace minerals. In muscle tissues, B, Fe and Mn contents were lower and Cu and Zn contents higher, in starved animals than in fed animals (p < .05). Significant negative correlations between the digestive gland index and the contents of Cu, Fe and Zn suggested that the loss of weight of the digestive gland during starvation is responsible for this increase in mineral concentration. This and further information on variation in mineral content will help to optimize performance of diets formulated for the octopus.  相似文献   

Histopathological Changes in Third-Instar and Adult Anastrepha ludens (Diptera: Tephritidae) After in vitro Heat Treatment     

Lorena Caro-Corrales  Jose Caro-Corrales  Angel Valdez-Ortiz  Jose Lopez-Valenzuela  Hector Lopez-Moreno  Daniel Coronado-Velazquez  Emilio Hernandez-Ortiz  Jose Rendon-Maldonado 《Journal of insect science (Online)》2015,15(1)

The Mexican fruit fly, Anastrepha ludens Loew (Diptera: Tephritidae), is one of the most harmful pests of mango causing direct damage by oviposition on the fruit pulp. Mango for export is subjected to hydrothermal treatment as a quarantine method for the control of this pest, but exposure to heat for long periods of time reduces considerably the quality and shelf-life of treated fruit. The aim of this work was to study morphological changes of third-instar larvae and adults of A. ludens after in vitro exposure to high temperature at sublethal times. A heating block system was used to expose larvae at 46.1°C for 19.6 and 12.9 min, producing 94.6 and 70% mortality, respectively. Treated larvae were processed for optical microscopy. A fraction of surviving treated larvae was separated into containers with artificial diet to allow development into adults. Adult sexual organs were dissected and processed for transmission electron microscopy analysis. Results showed that 94.6% of the treated larvae died at 46.1°C for 19.6 min and none of the surviving larvae eclosed to adulthood, as they developed as malformed puparia. For the in vitro treatment at 46.1°C during 12.9 min, 70% of the treated larvae died and only 3.75% reached the adult stage, but ultrastructural damage in the male testes and in the female ovaries was observed. Additionally, 11.1% of the adult flies from the in vitro treatment also showed wing malformation and were incapable of flying. The analysis showed that surviving flies were unable to reproduce.  相似文献   

Inclusion of fish and krill meal in extruded diets for Octopus vulgaris (Cuvier, 1797): assessment of acceptance     

Pablo Querol  Piedad S Morillo‐Velarde  Jesus Cerezo Valverde  Martinez Llorens Silvia  Andres Vicente Moñino  Miguel Jover Cerdá  Ana Tomás Vidal 《Aquaculture Research》2014,45(8):1421-1424

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Determination of organic acids in tissues and exudates of maize, lupin, and chickpea by high-performance liquid chromatography-tandem mass spectrometry     

Erro J  Zamarreño AM  Yvin JC  Garcia-Mina JM 《Journal of agricultural and food chemistry》2009,57(10):4004-4010

This article describes a fast and simple methodology for the extraction and determination of organic acids in tissues and root exudates of maize, lupin, and chickpea by LC/MS/MS. Its main advantage is that it does not require sample prepurification before HPLC analysis or sample derivatization to improve sensibility. The results obtained showed good precision and accuracy, a recovery close to 100%, and no significant matrix effect. Moreover, the sensibility of the method is in general better than that of previously described methodologies, with detection limits between 15 and 900 pg injected.  相似文献   

Fine-Scale Spatial Genetic Structure in Perennial Grasses in Three Environments     

Steven E. Smith  Tulio Arredondo  Martín Aguiar  Elisabeth Huber-Sannwald  Angel Alpuche  Armando Aguado  Oscar A. Grageda  Kandres Halbrook  Cecilia Bottini 《Strength and Conditioning Journal》2009,62(4):356-363

Past research has shown that changes in grazing-resistance traits may be associated with genetic changes in plant populations. Little is known about spatial genetic relationships within plant populations (spatial genetic structure) and any grazing effects on these relationships. Here we present observations of the fine-scale spatial genetic structure in three grass species in semiarid environments (Arizona, Mexico, and Argentina). In each environment, populations of a dominant grass species were sampled from two sites with contrasting livestock grazing histories. Plant genotypes were described with the use of amplified fragment length polymorphism markers. In Arizona, populations of sideoats grama (Bouteloua curtipendula var. caespitosa Gould and Kapadia) differed in that one has never experienced livestock grazing, whereas cattle have grazed the other. In the other two environments, populations exposed to long-term heavy grazing were examined, along with those that experienced either moderate grazing (Mexico, blue grama [Bouteloua gracilis {Willd. ex Kunt} Lag. ex Griffiths]) or extended exclusion of livestock (Argentina [Poa ligularis Nees ex Steud.]). Based on independent analysis of each population, we observed no differences in average gene diversity between populations of each species. With the use of analysis of molecular variance we found slight but significant genetic differentiation between populations with different grazing histories in Arizona and Argentina. Significant genetic structure was present in all populations and indicated an inverse relationship between spatial and genetic distance. Interestingly, this relationship was most pronounced in the cattle-free sideoats grama population, suggesting larger genetic neighborhood areas in the absence of livestock. Less distinct differences in spatial genetic structure associated with grazing history were evident in the other two species. We hypothesize that livestock grazing may lead to increased homogeneity in genetic structure at the landscape scale. Effectively examining this hypothesis presents many experimental challenges.  相似文献