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91.
92.
Infective third stage larvae (L3) of Trichostrongylus vitrinus were radiolabelled with 75 selenium by a method which did not affect their viability. Three groups of six-month-old lambs were infected daily with 1000 L3 for four, eight and 12 weeks, respectively. After each period, one of those groups (n = 5) and a group (n = 4) of worm-free controls were challenged with three consecutive daily doses of 1000 radiolabelled L3, killed 10 days after the first dose, and their worm burdens examined. After four weeks of continuous infection partial immunity to the establishment of challenge L3 was apparent, and by eight and 12 weeks, with the exception of one sheep, there was almost total resistance to incoming worms. Immunity was also expressed as an inhibition of the development of established worms. 相似文献
93.
Enteric campylobacter infection in gnotobiotic calves and lambs 总被引:1,自引:0,他引:1
Gnotobiotic calves and lambs were infected orally with Campylobacter jejuni, C coli or C hyointestinalis to assess pathogenicity. All animals were successfully colonised and excreted mucoid faeces but showed no other clinical signs. Campylobacters colonised the large intestine better than the small intestine, in which bacterial numbers decreased with time after infection. Campylobacters were found occasionally in the lumen of crypts in close proximity to epithelial cells and included in a mucus-like material. Lesions were mostly in the large intestine in calves whereas in lambs they were present in the ileum. In animals inoculated with C jejuni or C coli scattered crypt abscesses, focal inflammatory infiltrates in the lamina propria and goblet cell discharge were found. In lambs inoculated with C hyointestinalis only minor changes were found in the small intestine. Serum antibody response was either absent or present at a low level only from the 19th day after infection. 相似文献
94.
95.
Petrásek J Mravec J Bouchard R Blakeslee JJ Abas M Seifertová D Wisniewska J Tadele Z Kubes M Covanová M Dhonukshe P Skupa P Benková E Perry L Krecek P Lee OR Fink GR Geisler M Murphy AS Luschnig C Zazímalová E Friml J 《Science (New York, N.Y.)》2006,312(5775):914-918
Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport-related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux. 相似文献
96.
Best A Clifford D Crudgington B Cooley WA Nunez A Carter B Weyer U Woodward MJ La Ragione RM 《Veterinary research》2009,40(1):9-Feb;40(1):09
In cattle, the lymphoid rich regions of the rectal-anal mucosa at the terminal rectum are the preferred site for Escherichia coli O157:H7 colonisation. All cattle infected by rectal swab administration demonstrate long-term E. coli O157:H7 colonisation, whereas orally challenged cattle do not demonstrate long-term E. coli O157:H7 colonisation in all animals. Oral, but not rectal challenge of sheep with E. coli O157:H7 has been reported, but an exact site for colonisation in sheep is unknown. To determine if E. coli O157:H7 can effectively colonise the ovine terminal rectum, in vitro organ culture (IVOC) was initiated. Albeit sparsely, large, densely packed E. coli O157:H7 micro-colonies were observed on the mucosa of ovine and control bovine terminal rectum explants. After necropsy of orally inoculated lambs, bacterial enumeration of the proximal and distal gastrointestinal tract did suggest a preference for E. coli O157:H7 colonisation at the ovine terminal rectum, albeit for both lymphoid rich and non-lymphoid sites. As reported for cattle, rectal inoculation studies were then conducted to determine if all lambs would demonstrate persistent colonisation at the terminal rectum. After necropsy of E. coli O157:H7 rectally inoculated lambs, most animals were not colonised at gastrointestinal sites proximal to the rectum, however, large densely packed micro-colonies of E. coli O157:H7 were observed on the ovine terminal rectum mucosa. Nevertheless, at the end point of the study (day 14), only one lamb had E. coli O157:H7 micro-colonies associated with the terminal rectum mucosa. A comparison of E. coli O157:H7 shedding yielded a similar pattern of persistence between rectally and orally inoculated lambs. The inability of E. coli O157:H7 to effectively colonise the terminal rectum mucosa of all rectally inoculated sheep in the long term, suggests that E. coli O157:H7 may colonise this site, but less effectively than reported previously for cattle. 相似文献
97.
Burns M Crossan AN Kennedy IR Rose MT 《Journal of agricultural and food chemistry》2008,56(13):5260-5265
This paper investigates the potential use of composted cotton gin trash (CCGT) as a pesticide sorption medium in remediation of contaminated tailwater. CCGT was found to contain a large organic matter fraction (25.22%). Sorption of endosulfan sulfate and diuron, using the batch equilibrium method, was rapid but not limited for the range of applied concentrations, with diuron failing to reach equilibrium after two days. The partition K d and organic carbon partition K(OC) coefficients determined diuron ( Kd = 78; K(OC) = 526) and endosulfan sulfate ( Kd = 1500; K(OC) = 10,111) to reside in the solid phase. Limited desorption of diuron and higher range concentrations of endosulfan sulfate (50-100 microg L(-1)) were quantified. Sorption and desorption resulted from hydrophobic and hydrophilic interactions with the humic components of the compost. CCGT was concluded to have a superior sorption capacity to other sorbents reported in the literature, an assessment that requires field substantiation. 相似文献
98.
BACKGROUND: Pyrethroid insecticides have historically been effective for controlling bean leaf beetles in soybeans. Perceived field efficacy in the Mississippi Delta region has been lower over the past several years. Laboratory and field data were collected to determine whether there has been a reduction in pyrethroid insecticide susceptibility. RESULTS: Laboratory and field data both confirmed that control of bean leaf beetles with pyrethroid insecticides is poorer in the Delta regions of Mississippi and Louisiana than in all other regions tested. However, it was not possible to show that this reduced susceptibility changed over time. Furthermore, insect movement appeared to make insecticide susceptibility regional, so that localized insecticide applications had little impact on the susceptibility of successive generations. CONCLUSION: Lack of field control owing to low efficacy of pyrethroid insecticides against bean leaf beetle populations in the Delta region should be expected. Selection pressure on a regional basis may further decrease efficacy. Carbamate insecticides generally still provide high levels of control. Copyright © 2011 Society of Chemical Industry 相似文献
99.
Van Kerkhove MD Riley S Lipsitch M Guan Y Monto AS Webster RG Zambon M Nicoll A Peiris JS Ferguson NM 《Science (New York, N.Y.)》2012,336(6088):1506; author reply 1506
A better understanding of the severity of H5N1 in humans is needed. Wang et al. (Brevia, 23 March 2012, p. 1463; published online 23 February 2012) overinterpret the results of seroprevalence studies and take too little account of underlying uncertainties. Although the true risk of death from H5N1 infection will likely be lower than the 60% of reported laboratory-confirmed cases, there is little evidence of millions of missed infections. 相似文献
100.
F W Boebel F A Ehrenford G M Brown R D Angus C O Thoen 《Journal of the American Veterinary Medical Association》1979,175(3):276-277
In a serologic and blood culture survey for Brucella canis in 2,572 pound dogs from eight Illinois and Wisconsin counties, 173 (6.7%) were reactive in a presumptive slide test, 41 (1.5%) were reactive in a tube agglutination test, and 6 (0.2%) were positive in blood culture. 相似文献