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A simple trapping procedure for concentration of volatile organic compounds present in a head space and the subsequent analysis of the retained compounds by combined gas chromatographymass spectrometry is described. The procedure was used to study the organic metabolites evolved from various soils when they were amended with glucose and incubated under argon. A number of organic acids, alcohols, aldehydes and esters were evolved during a 4 week period. Less than 10 per cent of the added carbon was recovered as organic compounds, which was largely due to poor recovery of volatile organic acids from soil. Some implications of the observations to microbial ecology are discussed.  相似文献   
23.
Marine algae are an excellent source of novel lectins. The isolation of lectins from marine algae expands the diversity in structure and carbohydrate specificities of lectins isolated from other sources. Marine algal lectins have been reported to have antiviral, antitumor, and antibacterial activity. Lectins are typically isolated from marine algae by grinding the algal tissue with liquid nitrogen and extracting with buffer and alcohol. While this method produces higher yields, it may not be sustainable for large-scale production, because a large amount of biomass is required to produce a minute amount of compound, and a significant amount of waste is generated during the extraction process. Therefore, non-destructive extraction using algal culture water could be used to ensure a continuous supply of lectins without exclusively disrupting the marine algae. This review discusses the traditional and recent advancements in algal lectin extraction methods over the last decade, as well as the steps required for large-scale production. The challenges and prospects of various extraction methods (destructive and non-destructive) are also discussed.  相似文献   
24.
Cultivated pearls frequently exhibit morphological irregularities making obvious that mineral deposition was irregularly distributed onto nucleus surface. Taking advantage of experimental cultivations with short durations (from 10 days to few months), these irregular deposits predating occurrence of the nacre were investigated in Polynesian pearls by biochemical characterizations and a series of physical methods. Diversity in the resulting data suggests that various in‐depth alterations of the biomineralization mechanism may have occurred during the grafting process, leading to diversity in the biochemical pathways to nacreous deposition. This allows a precise discussion of current views about pearl formation. The “reversed shell theory” is formally disproved through point to point comparison with development of the shell growing edge. Similarity of pearl formation with “regeneration” or “shell repair” is also discussed, emphasizing the differences between these concepts.  相似文献   
25.
Comparisons were made between the amounts of abamectin and total radioactivity recovered from Spodoptera littoralis and Heliothis armigera larvae after topical application of [3H]abamectin. Penetration (as shown by wash-off experiments) did not differ significantly between the Spodoptera instars. Significantly more abamectin was recovered from ventral nerve cord samples of larvae showing symptoms of poisoning than from larvae not showing these symptoms. Fifth-instar S. littoralis larvae had a significantly lower proportion of radioactivity as abamectin in the ventral nerve cord than in sixth-instar S. littoralis or fifth- and sixth-instar H. armigera. The proportion of radioactivity present as abamectin (but not total radioactivity) was significantly increased when the fifth-instar S. littoralis larvae were pre-treated with piperonyl butoxide (PB) suggesting that the relative insensitivity of fifth-instar S. littoralis larvae to abamectin is due at least in part to greater metabolism, particularly by microsomal oxidases. Fat body samples consistently had a greater proportion of radioactivity as abamectin than the nerve cord and the former may act as storage sites for abamectin.  相似文献   
26.
Physical therapy is a profession with an established scientific basis in human beings and companion animals. It has a large number of clinical applications in the restoration, maintenance, and promotion of optimal physical function. In providing physical therapy, the goal is to restore, maintain, and promote optimal function, optimal fitness, wellness, and quality of life as they relate to movement disorders and health. A major emphasis is to prevent or minimize the onset, clinical signs, and progression of impairments, functional limitations, and disabilities that may result from diseases, disorders, conditions, and injuries.  相似文献   
27.
Owners and trainers exercise sporting dogs to increase their fitness and optimize their conditioning and performance. Training is designed to in-crease strength, endurance, and agility and is sport-specific. Sporting dogs are susceptible to specific musculoskeletal injuries. The rehabilitation of sporting dogs after these injuries follows specific principles during the acute, subacute, and reconditioning periods.  相似文献   
28.
Update on mastitis. III. Mastitis therapy   总被引:1,自引:0,他引:1  
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The field life for contact activity of cypermethrin and permethrin, applied to pasture, was similar for all of five application rates between 0.25 and 1.5 kg ha?1. Laboratory bioassays with grass grub beetles, Costelytra zealandica (White), showed that the insecticides lost their activity after about 4–9 days on pasture. Microencapsulation significantly increased the active field life of permethrin.  相似文献   
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