Finances. My client's ABC: from which customers do I actually make a living?     

Racine A 《Schweizer Archiv für Tierheilkunde》2008,150(8):423-424

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Development to Term of Fused and Partially Enucleated Mouse Two-Cell Embryos     

Annette Clement-Sengewald  Gottfried Brem 《Reproduction in domestic animals》1990,25(1):14-21

Contents: After the blastomeres of mouse two-cell embryos were fused by electric pulses within the zona pellucida, one nucleus of the fusion products was removed following the enucleation method described by McGrath and Solter (1983a, 1983b). 38% (196/520) of the fused embryos were enucleated successfully when Whitten's medium was used as enucleation medium and 434 of 1007 (43%) of the embryos when M 2 was used. 30% (47/159) of the partially enucleated embryos cleaved during their in vitro cultivation but only 3% developed to the morula or blasto cyst stage. 20 young (17%) were born after the transfer of 120 fused and partially enucleated two-cell embryos to 8 pseudopregnant recipients. It was shown that fused and partially enucleated two-cell embryos are able to survive and are able to reach adulthood, although their developmental rate is significantly lower than that of control embryos .
Here we report experiments for the examination of the developmental capacity of two-cell mouse embryos partially enucleated after fusion .  相似文献   

Critically appraising studies reporting assessing diagnostic tests.     

Annette O'Connor  Richard B Evans 《Veterinary Clinics of North America: Small Animal Practice》2007,37(3):487-497

Studies that report the sensitivity and specificity of diagnostic tests are susceptible to flaws that can introduce bias and lead to incorrect estimates. This article uses the quality assessment of diagnostic accuracy studies checklist to describe how to appraise a study reporting diagnostic test comparisons critically. The article also contains a glossary of terms that are useful in discussions about diagnostic tests.  相似文献   

Parasite population dynamics in pigs infected with Trichuris suis and Oesophagostomum dentatum     

Heidi Huus Petersen  Annette Andreasen  Helene Kringel  Allan Roepstorff  Stig M. Thamsborg 《Veterinary parasitology》2014,199(1-2):73-80

The aim of the present study was to investigate the population dynamics and potential interactions between Trichuris suis and Oesophagostomum dentatum in experimentally co-infected pigs, by quantification of parasite parameters such as egg excretion, worm recovery and worm location. Forty-eight helminth naïve pigs were allocated into four groups. Group O was inoculated with 20 O. dentatum L₃/kg/day and Group T with 10 T. suis eggs/kg/day. Group OT was inoculated with both 20 O. dentatum L₃/kg/day and 10 T. suis eggs/kg/day, while Group C was kept as an uninfected control group. All inoculations were trickle infections administered twice weekly and were continued until slaughter. Faecal samples were collected from the rectum of all pigs at day 0, and twice weekly from 2 to 9 weeks post first infection (wpi). Six pigs from each group were necropsied 5 wpi and the remaining 6 pigs from each group were necropsied 10 wpi. The faecal egg counts (FEC) and total worm burdens of O. dentatum were dramatically influenced by the presence of T. suis, with significantly lower mean FECs and worm burdens at 5 and 10 wpi compared to single infected pigs. Furthermore, in the presence of T. suis we found that O. dentatum was located more posteriorly in the gut. The changes in the Trichuris population were less prominent, but faecal egg counts, worm counts 5 wpi (57% recovered vs. 39%) and the proportion of infected animals at 10 wpi were higher in Group OT compared to Group T. The location of T. suis was unaffected by the presence of O. dentatum. These results indicate an antagonistic interaction between T. suis and O. dentatum which is dominated by T. suis.  相似文献   

CD4+ and CD8+ skin‐associated T lymphocytes in canine atopic dermatitis produce interleukin‐13, interleukin‐22 and interferon‐γ and contain a CD25+FoxP3+ subset     

Annette Jassies‐van der Lee  Victor P. M. G. Rutten  Jet Bruijn  Ton Willemse  Femke Broere 《Veterinary dermatology》2014,25(5):456-e72

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Strong association of de novo copy number mutations with autism     

Sebat J  Lakshmi B  Malhotra D  Troge J  Lese-Martin C  Walsh T  Yamrom B  Yoon S  Krasnitz A  Kendall J  Leotta A  Pai D  Zhang R  Lee YH  Hicks J  Spence SJ  Lee AT  Puura K  Lehtimäki T  Ledbetter D  Gregersen PK  Bregman J  Sutcliffe JS  Jobanputra V  Chung W  Warburton D  King MC  Skuse D  Geschwind DH  Gilliam TC  Ye K  Wigler M 《Science (New York, N.Y.)》2007,316(5823):445-449

We tested the hypothesis that de novo copy number variation (CNV) is associated with autism spectrum disorders (ASDs). We performed comparative genomic hybridization (CGH) on the genomic DNA of patients and unaffected subjects to detect copy number variants not present in their respective parents. Candidate genomic regions were validated by higher-resolution CGH, paternity testing, cytogenetics, fluorescence in situ hybridization, and microsatellite genotyping. Confirmed de novo CNVs were significantly associated with autism (P = 0.0005). Such CNVs were identified in 12 out of 118 (10%) of patients with sporadic autism, in 2 out of 77 (3%) of patients with an affected first-degree relative, and in 2 out of 196 (1%) of controls. Most de novo CNVs were smaller than microscopic resolution. Affected genomic regions were highly heterogeneous and included mutations of single genes. These findings establish de novo germline mutation as a more significant risk factor for ASD than previously recognized.  相似文献   

Biomechanical properties of canine staphylectomies closed with barbed or smooth suture     

Hadley E. Gleason  Heidi Phillips  Annette M. McCoy  Santiago D. Gutierrez‐Nibeyro  Brendan C. McKiernan  Daniel J. Duffy  Ian M. Feign  Woojae Jason Kim  Mariana E. Kersh 《Veterinary surgery : VS》2021,50(1):196-206

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Evaluation of effects of transgenic Bt maize on microarthropods in a European multi-site experiment   总被引：2，自引：5，他引：2  

Jrme Cortet  Bryan S. Griffiths  Marko Bohanec  Damjan Demsar  Mathias N. Andersen  Sandra Caul  Andrew N. E. Birch  Cline Pernin  Elisabeth Tabone  Annette de Vaufleury  Xin Ke  Paul Henning Krogh 《Pedobiologia》2007,51(3):207-ECOGEN

The effects of maize expressing the Bacillus thuringiensis Cry1Ab protein (Bt maize) on soil microarthropods were assessed in the field at four European locations (two in Denmark and two in France) that differ in their climatic conditions or soil properties. Each site was considered as a separate experiment, with separate statistical comparison. Effects of farming practices using Bt maize were compared with conventional farming practices using near-isogenic non-Bt maize and also (at some of the sites) other conventional varieties. Furthermore, at one field site (Foulum, Denmark), the effects of Bt crops were studied in both conventional tillage and reduced tillage contexts. At another field site (Askov, Denmark), Bt maize effects were also compared to the effects of the chemical insecticide dimethoate. Moreover, at three of the field sites (all except Narbons, France), the possibility of a localised Bt effect around the rhizosphere compared to the bulk soil was assessed by sampling within and between maize rows. There were some significant negative effects of Bt maize on microarthropods in soils with a high clay content. Significant differences of the same magnitude also occurred between different conventional varieties of maize, but the effect of dimethoate appeared clearly greater than Bt effects. It is thus debatable if the Bt maize effect is an effect of the Bt toxin or just an effect of the maize variety. Based on the results, it can be concluded that the effect of Bt maize on soil microarthropods was small and within the normal variation expected in conventional agricultural systems.  相似文献   

Transgenic fertile plants of soybean [Glycine max (L.) Merrill] obtained from bombarded embryogenic tissue     

Annette Droste  Giancarlo Pasquali  Maria Helena Bodanese-Zanettini 《Euphytica》2002,127(3):367-376

This work describes the production of transgenic, fertile plants of soybean [Glycine max (L.) Merrill]. The transformation method combines the advantages of somatic embryo genesis with the efficiency of particle bombardment of tissues that have a great capacity for in vitro proliferation and regeneration. The results described here represent the first report of transformation of soybean cultivars recommended for commercial growing in South Brazil using somatic embryogenesis, and may open the field for the improvement of this crop in this country by genetic engineering. This revised version was published online in August 2006 with corrections to the Cover Date.  相似文献   

Effect of radiotherapy on freedom from seizures in dogs with brain tumors     

Susana R. Monforte Monteiro  John H. Rossmeisl  Jason Russell  Mark A. Holmes  Annette Wessmann  Jo Morris  Jane M. Dobson  An E. Vanhaesebrouck 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(2):821-827

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