Characterization of the extracellular matrix in the chorioallantoic membrane of water buffalo (Bubalus bubalis) in early gestation     

Jssica Borghesi  Higor da Silva Ferreira  Phelipe Oliveira Favaron  Lara Carolina Mario  Adriana Raquel de Almeida da Anunciao  Franceliusa Delys de Oliveira  Rafael Gonalves Hayashi  Adriana Caroprezo Morini  Maria Angelica Miglino 《Reproduction in domestic animals》2019,54(10):1313-1321

Placenta is formed by a parenchyma rich in extracellular matrix (ECM), and this structure guarantees the proper development of the embryo and placental functioning. Recently, studies have focused on the characterization of ECM in the placenta and foetal membranes of different species. This work aimed to analyse the composition of the ECM and to quantify the types of collagens in its composition. For this, 33 chorioallantoic membranes were used at different gestational ages, which were grouped into five groups. Subsequently, haematoxylin–eosin staining, Masson trichrome and picrosirius were performed for histological analysis. Through the technique of polarized light, it was possible to quantify the total collagen present in the membranes and finally the immunohistochemical technique was performed to verify the presence of collagens and glycoproteins. It was possible to verify that the chorioallantoic membranes have, in all the gestational periods of the initial third of gestation, the same histological structures, being the most significant difference the membrane thickening that occurs gradually during the gestation. However, we notice the appearance of binucleate cells only from group II. In addition, it was verified that a gradual increase of collagen occurs until the group IV, yet from the group V begins to occur a decrease of this protein. In addition, collagen I, collagen III, fibronectin and laminin were present in all membranes. With this, we concluded that the buffalo chorioallantoic membrane presents ECM in constant remodelling at the beginning of gestation and can be used as biomaterial in works on regenerative biology.  相似文献   

Contrasting effects of heat shock during in vitro maturation on development of in vitro‐fertilized and parthenogenetic bovine embryos     

Luiz Sergio Almeida Camargo  Fernanda Queiros Costa  Michele Munk  Sabine Wohlres‐Viana  Raquel Varela Serapio  Bruno Campos Carvalho  Paulo Henrique Campos  Alex Cabral Vieira  Luiz Altamiro Garcia Nogueira  Joao Henrique Moreira Viana 《Reproduction in domestic animals》2019,54(10):1357-1365

This study investigated the influence of heat shock during in vitro maturation on embryo development following in vitro fertilization (IVF) or parthenogenesis (Part). Immature bovine cumulus–oocyte complexes were exposed to heat shock (41.0°C) during the first 12 hr of in vitro maturation (IVM), followed by 12 hr at 38.5°C. Control group consisted of in vitro maturation for 24 hr at 38.5°C. Oocytes were in vitro‐fertilized or activated with ionomycin and cultured in vitro for 192 hr post‐in vitro insemination or parthenogenetic activation (hpia). There was an interaction (p < .01) between temperature of IVM and method of oocyte activation (IVF or Part) for cleavage at 48 hpia. Heat shock had a negative impact (p < .01) on cleavage of IVF embryos, whereas no (p > .05) effect was found in the Part embryos. Embryo development towards blastocyst stage at 168 and 192 hpia decreased in both IVF and Part embryos derived from heat‐shocked oocytes. Heat shock increased (p < .05) the apoptotic index in Part blastocysts, but no effect (p > .05) was found in IVF counterparts. Heat shock also down‐regulated the expression of AQP3 (p < .01) and up‐regulated the expression of HSP70.1 (p < .01) in Part blastocysts, whereas it down‐regulated the expression of ATP1A1 (p < .05) in IVF blastocysts. In conclusion, the effects of heat shock during IVM on early embryo cleavage and blastocyst apoptosis are influenced by the method of oocyte activation and expression of some genes can be disturbed in embryos derived from heat‐shocked oocytes.  相似文献   

Alternatives in Donkey semen cryopreservation: Mare vs. Jenny Colostrum     

Cristina lvarez  Noelia Gonzlez  Victoria Luo  Felisa Martínez  Lydia Gil 《Reproduction in domestic animals》2019,54(Z4):94-97

The aim of this study was to test and compare two new components in extenders for freezing donkey semen: mare colostrum and jenny colostrum. Colostrum was obtained from four mares and four jennies right after the foal's birth. Ejaculates were collected from five fertile donkeys. Sperm samples were pooled, diluted and cryopreserved in three different experimental extender groups: lactose supplemented with egg yolk extender (20%) as the control group, lactose supplemented with jenny colostrum extender (20%), and lactose supplemented with mare colostrum extender (20%). After thawing, we evaluated the sperm motility by means of computer‐assisted analysis, viability by SYBR‐14 and propidium iodide (PI), membrane functional by HOS test and acrosome integrity by isothiocyanate conjugated with peanut agglutinin (FITC‐PNA) and PI. The results demonstrated that lactose–jenny colostrum extender displayed significantly higher values (p < .05) in nearly all parameters evaluated – Total Motility, Viability, HOS test, VCL, VSL, VAP, LIN, STR and WOB –, compared with mare colostrum and egg yolk extenders after thawing. In conclusion, the extender containing jenny colostrum used for donkey semen cryopreservation improved the donkey sperm quality after the freezing–thawing process.  相似文献   

A kinematic comparison of the locomotor pattern of horses sedated with detomidine alone and in combination with low doses of butorphanol     

M. A. Frigerio  D. Gómez Cisneros  I. Santiago Llorente  G. Manso-Díaz  J. López-Sanromán 《Equine veterinary journal》2019,51(6):825-830

  相似文献   

Reproductive seasonality in Saanen goats kept under tropical conditions     

Balaro  Mario Felipe A.  de Mello  Samuel Guaraná Valverde  da Silva Santos  Alex  Cavalcanti  Luiza Mattos  Almosny  Nádia Regina Pereira  Fonseca  Jeferson F.  Brandão  Felipe Z. 《Tropical animal health and production》2019,51(2):345-353

Tropical Animal Health and Production - This study aimed to verify the reproductive seasonality in Saanen goats from distinct parity orders (nulliparous, primiparous, and pluriparous) throughout an...  相似文献   

Ruminal and morphometric parameters of the rumen and intestines of sheep fed with increasing levels of spineless cactus (Nopalea cochenillifera Salm-Dyck)     

Lima  Talma Jordana  Costa  Roberto Germano  de Medeiros  Geovergue Rodrigues  de Medeiros  Ariosvaldo Nunes  Ribeiro  Neila Lidiany  de Oliveira  Juliana Silva  Guerra  Ricardo Romão  de Carvalho  Francisco Fernando Ramos 《Tropical animal health and production》2019,51(2):363-368

Tropical Animal Health and Production - The objective of this study was to evaluate the ruminal parameters (pH, N-NH3, and microbial protein) and morphometry of the rumen and intestine of sheep fed...  相似文献   

Short communication: detection and molecular characterization of hepatitis E virus in domestic animals of São Tomé and Príncipe     

Mesquita  João Rodrigo  Istrate  Claudia  Santos-Ferreira  Nânci L.  Ferreira  Ana S.  Abreu-Silva  Joana  Veiga  José  van der Poel  Wim H. M.  Nascimento  Maria S. J. 《Tropical animal health and production》2019,51(2):481-485

Tropical Animal Health and Production - As in most of the African continent, the status of hepatitis E virus (HEV) infection in domestic animals in São Tomé and Príncipe, an...  相似文献   

Behavioral and physiological evaluation of sows raised in outdoors systems in the Brazilian semiarid region     

de Melo  Rafaela Leitão Correia  Dutra Júnior  Wilson Moreira  Palhares  Liliane Olímpio  de Moura Ferreira  Débora Nathália  de Aquino  Rafael Santos  Cordeiro Manso  Helena Emília Cavalcanti 《Tropical animal health and production》2019,51(5):1057-1063

Tropical Animal Health and Production - The objective of this study was to evaluate the biochemical, behavioral, and physiological parameters in pregnant sows of native and improved crossbreeds...  相似文献   

Relationship between performance,metabolic profile,and feed efficiency of lactating beef cows     

Souza  Luana Lelis  Zorzetto  Mariana Furtado  Ricci  Túlio José Terra  Canesin  Roberta Carrilho  Dias e Silva  Nhayandra Christina  Negrão  João Alberto  dos Santos Gonçalves Cyrillo  Joslaine Noely  Mercadante  Maria Eugênia Zerlotti 《Tropical animal health and production》2019,51(7):2045-2055

Tropical Animal Health and Production - Twenty-seven Nellore cow-calf pairs were submitted for feed efficiency testing. The animals were weighed every 21 ± 5&nbsp;days to...  相似文献   

Performance,breast meat yield and abdominal fat deposition of male broiler chickens fed diets supplemented with DL‐xnethionine or DL‐xnethionine hydroxy analogue free acid     

E. Esteve‐Garcia  LL. Llauradó 《British poultry science》1997,38(4):397-404

1. An experiment was conducted to compare the relative bioefficacy of DL‐methionine hydroxy analogue free acid (DL‐MHA‐FA) with DL‐methionine in broiler chickens. Responses used for comparison were weight gain and food efficiency between 7 and 35 d of age, and breast meat deposition, food cost per kg of breast meat, and abdominal fat at 41 d of age.

2. A total of 2160 seven‐day‐old male broiler chicks were used. The feeding programme consisted of a starter diet from 7 to 21 d, and a finisher diet till the end of the experiment. The starter basal diet contained 6.1 g/kg total sulphur‐containing amino acids (TSAA), and an estimated metabolisable energy (ME) content of 13.2 MJ/kg. The finisher diet contained 5.8 g/kg TSAA and an estimated ME content of 13.6 MJ/kg. Four concentrations of DL‐methionine and DL‐MHA‐FA were added at 0.5g/kg increments on an equimolar basis. Therefore, there were 9 experimental treatments which were each applied to 6 replicates of 40 chicks. Weight gain and food efficiency were determined at 35 d of age. Breast yield and carcase fat were measured at 41 d.

3. Significant responses to graded amounts of both methionine sources were observed in weight gain, food efficiency, breast meat percentage, and food cost per kg of breast meat. The responses fitted exponential regression curves. Based on the regression coefficients, equimolar bioefficacy of DL‐MHA‐FA relative to DL‐methionine was 80% for daily gain, 83% for food efficiency, 51% for breast meat yield, and 66% for food cost per kg of breast meat. Differences between the 2 sources were significant (P< 0.05) for breast meat yield and food cost per kg of meat and (P< 0.10) for food efficiency.  相似文献