High-resolution protein structure determination by serial femtosecond crystallography     

Boutet S  Lomb L  Williams GJ  Barends TR  Aquila A  Doak RB  Weierstall U  DePonte DP  Steinbrener J  Shoeman RL  Messerschmidt M  Barty A  White TA  Kassemeyer S  Kirian RA  Seibert MM  Montanez PA  Kenney C  Herbst R  Hart P  Pines J  Haller G  Gruner SM  Philipp HT  Tate MW  Hromalik M  Koerner LJ  van Bakel N  Morse J  Ghonsalves W  Arnlund D  Bogan MJ  Caleman C  Fromme R  Hampton CY  Hunter MS  Johansson LC  Katona G  Kupitz C  Liang M  Martin AV  Nass K  Redecke L  Stellato F  Timneanu N  Wang D  Zatsepin NA  Schafer D  Defever J 《Science (New York, N.Y.)》2012,337(6092):362-364

Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.  相似文献   

Freezing stallion semen: Trial of an extender made with low density lipoproteins (LDL) from chicken egg yolk     

D. Moreno  D. Bencharif  L. Amirat-Briand  S. Destrumelle  M. Anton  P. Barriere  D. Tainturier 《Journal of Equine Veterinary Science》2012

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Amplification and sequencing of Brachyspira spp. specific portions of nox using paraffin-embedded tissue samples from clinical colitis in Austrian pigs shows frequent solitary presence of Brachyspiramurdochii     

Weissenböck H  Maderner A  Herzog AM  Lussy H  Nowotny N 《Veterinary microbiology》2005,111(1-2):67-75

Brachyspira infections are significant causes of enterocolitis in pigs. In order to differentiate pathogenic species (Brachyspira (Br.) hyodysenteriae, Brachyspira pilosicoli) from less pathogenic or non-pathogenic species (Brachyspira intermedia, Brachyspira innocens, Brachyspira murdochii) in paraffin-embedded tissue samples a polymerase chain reaction (PCR) protocol allowing identification of Brachyspira at species level in archival material was developed. This approach was complemented by sequencing of the PCR amplification products. All seven cases presented with clinical and morphological Brachyspira-associated enterocolitis. Br. hyodysenteriae was not identified in any of the cases, while Br. pilosicoli was identified in a single case in conjunction with Br. murdochii. One case each was found positive for Br. innocens and Br. intermedia. Interestingly, the majority of cases presented as single or double infections with Br. murdochii. In some of the pigs other pathogens, like porcine circovirus-2 or Lawsonia intracellularis were present. These observations point at the possibility that under certain conditions even Brachyspira species of low pathogenicity can multiplicate extensively and lead to Brachyspira-associated enterocolitis.  相似文献   

Oestrogen receptor genotypes and litter size in Hungarian Large White pigs   总被引：5，自引：0，他引：5  

G. Horogh  A. Zsolnai  I. Komlósi  A. Nyíri  I. Anton  & L. Fésüs 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2005,122(1):56-61

A total of 869 litter records of 226 Hungarian Large White sows have been analysed to investigate the possible use of the oestrogen receptor gene (ESR) as marker to improve litter size. First, second and later parities have been evaluated separately. Frequencies of A = 0.55 and B =0.45 have been calculated for the two ESR alleles and the observed/expected number of the three genotypes were as follows: AA: 71/69.1, AB: 108/111.8 and BB: 47/45.1. BB type first and later parity sows were superior to AB and AA sows for number born alive (NBA), total number of born (TNB) and the corrected number of weaned piglets (CNW), respectively.  相似文献   

Large breed health care symposium in Venice     

Beijer A 《Tijdschrift voor diergeneeskunde》2002,127(3):95

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Relation between Habitual Diet and Canine Mammary Tumors in a Case-Control Study   总被引：2，自引：0，他引：2  

Dolores Pérez Alenza  Gerard R. Rutteman  Laura Peña  Anton C. Beynen  Pedro Cuesta 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1998,12(3):132-139

In the present case-control study several dietary and nutritional factors were investigated to determine if a relationship exists between diet and development of mammary tumors in female dogs. Control female dogs (n = 86) were compared with a case group of dogs (n = 102) with dysplasias or tumors of the mammary gland. A questionnaire providing information on the dog's body conformation and dietary and reproductive histories was answered by the owners. Serum selenium and retinol concentrations and the fatty acid profile in subcutaneous adipose tissue were analyzed as indicators of nutritional status. Obesity at 1 year of age and 1 year before the diagnosis of mammary nodules was found to be significantly related to a higher prevalence of mammary tumors and dysplasias. The intake of homemade meals (compared to that of commercial foods) was also significantly related to a higher incidence of tumors and dysplasias. Other significant risk factors were a high intake of red meat, especially beef and pork, and a low intake of chicken. The subcutaneous fatty acid profile and the serum selenium concentration were not significantly different in the cases and the controls, with the exception of C18:1 fatty acid (oleic acid) content, which was significantly higher in the cases than in healthy controls. Serum retinol concentration was significantly lower in the cases than in the controls. In the multivariate analysis, older age, obesity at 1 year of age, and a high red meat intake were independently and significantly associated with the risk of developing mammary tumor and dysplasias.  相似文献   

An application of multilevel model for the analysis of factors influencing paddy field productivity in the Northern Vietnamese Mountains     

Hoang  Huong Thi Thu  Van Rompaey  Anton  Vu  Kim Chi  Nguyen  An Thinh  Vanacker  Veerle 《Paddy and Water Environment》2020,18(1):153-166

Paddy and Water Environment - In the Northern Vietnamese Mountains, paddy fields are the main source of foods and an important indicator to define wealth in ethnic communities. This paper deals...  相似文献   

Vegetationsökologischer Vergleich von Naturwald und Wirtschaftswald, dargestellt am Beispiel des Tilio-Carpinetum im Waldgebiet von Bialowieza/Nordost-Polen     

Anton Fischer  Clemens Abs  J. B. Falinski 《European Journal of Forest Research》1999,118(1-6):181-196

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Three-dimensional visualization of preferential flow patterns in two soils   总被引：3，自引：0，他引：3  

Anton W. J. Heijs  Coen J. Ritsema  Louis W. Dekker 《Geoderma》1996,70(2-4):101-116

Visualization techniques are helpful in finding spatial relationships of parameters which are otherwise difficult to detect. In this study, visualization techniques were used to examine the basis of moisture distribution patterns in a clay soil and a water repellent sandy soil. In the clay soil, spatial distributions of moisture contents and macropore networks were investigated with computed tomography (CT) images. The macropore networks were determined from CT images from a dry soil core, and the moisture content patterns from CT images after which KI enriched water was applied. Soil moisture and bulk density were investigated and measured in a water repellent sandy soil by intensive sampling of an area 1.2 m long and 0.6 m wide. The measured parameters were visualized in three dimensions using IRIS Explorer on a SGI-Indigo and a SGI-Onyx computer. The 3D computer representations showed that there exists a strong correlation between the moisture content distribution and the geometrical structure of the macropore network for a clay soil. Moisture contents higher than 30% were mostly found inside the upper part of the macropore network and lateral branches of the macropore network were significant drier than the downwards directed branches. The visualizations of the water repellent sandy soil revealed several 10 to 20 cm wide “fingers” starting around 10 cm depth at locations with the highest bulk densities.  相似文献   

The Chlamydomonas genome reveals the evolution of key animal and plant functions   总被引：1，自引：0，他引：1  

Merchant SS  Prochnik SE  Vallon O  Harris EH  Karpowicz SJ  Witman GB  Terry A  Salamov A  Fritz-Laylin LK  Maréchal-Drouard L  Marshall WF  Qu LH  Nelson DR  Sanderfoot AA  Spalding MH  Kapitonov VV  Ren Q  Ferris P  Lindquist E  Shapiro H  Lucas SM  Grimwood J  Schmutz J  Cardol P  Cerutti H  Chanfreau G  Chen CL  Cognat V  Croft MT  Dent R  Dutcher S  Fernández E  Fukuzawa H  González-Ballester D  González-Halphen D  Hallmann A  Hanikenne M  Hippler M  Inwood W  Jabbari K  Kalanon M  Kuras R  Lefebvre PA  Lemaire SD  Lobanov AV 《Science (New York, N.Y.)》2007,318(5848):245-250

Chlamydomonas reinhardtii is a unicellular green alga whose lineage diverged from land plants over 1 billion years ago. It is a model system for studying chloroplast-based photosynthesis, as well as the structure, assembly, and function of eukaryotic flagella (cilia), which were inherited from the common ancestor of plants and animals, but lost in land plants. We sequenced the approximately 120-megabase nuclear genome of Chlamydomonas and performed comparative phylogenomic analyses, identifying genes encoding uncharacterized proteins that are likely associated with the function and biogenesis of chloroplasts or eukaryotic flagella. Analyses of the Chlamydomonas genome advance our understanding of the ancestral eukaryotic cell, reveal previously unknown genes associated with photosynthetic and flagellar functions, and establish links between ciliopathy and the composition and function of flagella.  相似文献