排序方式： 共有22条查询结果，搜索用时 93 毫秒
11.
The methanol extract of stem barks of Alianthus excelsa was partitioned with chloroform. The chloroform extract showed fungistatic and fungicidal activity against Aspergillus niger, A. fumigatus, Penicillium frequentence, P. notatum and Botrytis cinerea. 相似文献
12.
Gopi B. Vir Singh Ran Kumar Satish Kumar Sushil Chauhan Anuj Sonwane Arvind Kumar Amit Bharati Jaya Vir Singh Shoor 《Veterinary research communications》2022,46(1):209-221
Paratuberculosis (PTB) is a chronic infectious enteritis of ruminants, caused by Mycobacterium avium subspecies paratuberculosis (MAP) that brings huge economic loss to the dairy farmers. The study was conducted to explore the association of selected SNPs in IFNG, SLC11A1, ANKRA2 and PGLYRP1 genes with resistance to PTB disease in Indian cattle population. A case-control resource population was established based on the results of diagnostic tests used for detection of MAP infection status viz. ELISA, Johnin PPD test, faecal microscopy and IS900 blood PCR. The PCR-RFLP method was used for genotyping of SNPs. SNPs rs109453173 in SLC11A1, rs110853455 in IFNG and rs41933863 in ANKRA2 genes were significantly (P<0.05) associated with resistance to MAP infection. For SNP rs109453173, GG genotype and G allele was found to be associated with resistance against MAP infection than CC and CG genotypes and C allele, respectively. For SNP rs110853455, AG genotype was found to be associated with susceptibility to MAP infection than AA and GG genotype. For SNP rs41933863, the AG genotype provided three and six times more resistance against MAP infection than GG and AA genotype. The results of this study are suggestive of SNPs rs109453173, rs110853455 and rs41933863 as potential markers for screening MAP resistant cattle and a breeding programme favouring GG genotype and G allele for rs109453173, AG genotype for rs41933863 and against AG genotype for rs110853455 might confer resistance against MAP infection in Indian cattle. However, investigation of these SNPs in an independent and larger population will warrant the strength of association for resistance against MAP infection in cattle.
相似文献13.
Anuj Rana Lata Nain Radha Prasanna Yashbir S. Shivay 《Soil Science and Plant Nutrition》2013,59(5):573-582
A pot experiment was undertaken under net house conditions, with three rhizobacterial strains AW1 (Bacillus sp.), AW5 (Providencia sp.) and AW7 (Brevundimonas sp.), applied along with 2/3 recommended dose of nitrogen (N) and full dose of phosphorus (P) and potassium (K) fertilizers (N90P60K60). An enhancement of 14–34% in plant biometric parameters and 28–60% in micronutrient content was recorded in treatments receiving the combination of AW1?+?AW5 strains, as compared to full dose of fertilizer application. The treatment involving inoculation with AW5?+?AW7 recorded highest values of % P and N, with a two-fold enhancement in phosphorus and 66.7% increase in N content, over full dose application of P and K fertilizers. A significant correlation was recorded between plant biomass, panicle weight, grain weight, N, P and iron (Fe) with acetylene reduction activity, indicating the significance of N fixation in overall crop productivity. Our study illustrates the multiple benefits of plant growth promoting rhizobacteria (PGPR) inoculation in integrated nutrient management and biofortification strategies for wheat crop. 相似文献
14.
Sen Chauhan Indra Gupta Ashok K. Khate Keviletsu Chauhan Anuj Krishna Shankar Rao Thakur Pathak Shivendra Hazra Ritwik Singh Maneesh 《Tropical animal health and production》2010,42(8):1809-1815
The objective of the study was to evaluate the effects of genetic and non-genetic factors on production of breeding bulls
and semen quality parameters in Karan Fries crossbred male by fitting least squares analysis. Genetically, the animals were
divided into three subclasses. The non-genetic factors were season of birth, period of birth, and age group with three subclasses
each for season of birth and period of birth. Age group was classified into four subclasses. The traits generated in the study
were number of males reaching semen donation stage (AFSC) and first freezing (AFSF), age at last semen collection (ALSC) and
last freezing (ALSF), age at disposal (AD), and lifetime semen production traits (up to 1 year after first freezing). The
effect of period of birth was significant for AFSC, AFSF, ALSC, and AD. It was also significant for total ejaculates produced
in a year. The age group had significant effect on AFSF. Effect of genetic group was significant for freezable ejaculates
produced in a year, for frozen semen doses produced in a year, and for number of ejaculates cryoprocessed in a year. Season
had no statistically significant effect on any of the traits studied. The influence of period revealed that the most of the
traits of breeding bulls improved after intermediate period, which could be due to better care, training, feeding, and other
management practices in the latter years. However, no consistent trend could be established for the effects of genetic groups
and other non-genetic causes on the traits considered. 相似文献
15.
Srinivasa Kamsagara Hanumanthappa Naveen Kumar Billekallu Thammegowda Prakash Patil Satish Rama Poojary Abhiman Purandara Ballyaya Ramesh Kavalagiriyanahalli Srinivasaiah Shankar Kalkuli M Anuj Tyagi Shanthanagouda Admane Holeyappa 《Aquaculture Research》2020,51(7):2863-2869
Early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) is currently the most significant disease of shrimp in farms of Vietnam, Thailand, Malaysia, China and Mexico, and there is a great risk that it may spread to other shrimp farming countries. Although, an array of sophisticated detection tools for AHPND available, there is a need for a sensitive, simple and rapid detection method. In this study, a simple, sensitive, rapid and polyclonal antibody‐based farmer‐friendly flow‐through assay (FTA) test has been developed for the detection of AHPND pathogen. The recombinant Photorhabdus insect‐related (Pir) A toxin‐like protein of AHPND pathogen was used to immunize rabbits at 21‐day interval observed for highest antibody titre after third booster by ELISA. The raised rabbit antiserum was purified by affinity chromatography and characterized by Western blot. The antiserum showed no cross‐reactivity with AHPND‐free Vibrio parahaemolyticus, V. anguillarum, White Spot Virus (WSV), Aeromonashydrophila and Aphanomycesinvadans. This polyclonal rabbit antiserum was used to develop a farmer‐friendly FTA test for the detection of AHPND pathogen. This simple FTA testis is more sensitive and could detect PirAVP toxin up to 0.121 µg/ml, compared with 0.242 µg/ml by immunodot assay. Furthermore, FTA test requires only 8–10 min for completion, compared with 3 hr by immunodot thus found to be more sensitive, specific and cost‐effective. Collectively, sensitive FTA test would help shrimp farmers to take real‐time management decisions, especially emergency harvest and finally be a better hope for the prevention of AHPND. 相似文献
16.
Prevalence and antimicrobial resistance of vibrios of human health significance in inland saline aquaculture areas 
下载免费PDF全文
下载免费PDF全文 Balwinder Singh Anuj Tyagi Naveen K Billekallu Thammegowda Meera D Ansal 《Aquaculture Research》2018,49(6):2166-2174
This study was conducted to evaluate the prevalence, potential pathogenicity and antimicrobial resistance of Vibrio isolates from 65 soil/water/fish samples collected from inland saline aquaculture areas. Depending on the sample type, presumptive Vibrio counts ranged from 2.50 to 6.16 log10 CFU/ml (or/g). Among the 119 confirmed Vibrio isolates, Vibrio cholerae was found to most dominant (91.6%) and it was detected in all the samples from inland saline areas. Seven other Vibrio spp. including Vibrio parahaemolyticus and Vibrio vulnificus were also detected. Except one O139 serotype, rest of the V. cholerae isolates were found belonging to non‐O1/non‐O139 serogroups. None of the V. cholerae isolate was found positive for ctx gene. Antimicrobial susceptibility testing against 7 commonly used antibiotics revealed highest resistance (50.4%) against ampicillin. Very high intermediate resistance (87.4%) was also observed against erythromycin. Contrary to previous studies, high susceptibility (>70%) to chloramphenicol, nalidixic acid, tetracycline and trimethoprim was observed in Vibrio isolates obtained in present study. Almost 20% of Vibrio isolates were resistant to two or more antibiotic classes with multiple antibiotic resistance (MAR) index value of ≥0.28. Presence of V. cholerae isolates with very high MAR index value of 0.85 also suggested that these multidrug‐resistant environment isolates could serve as reservoir of antibiotic‐resistant genes in aquatic systems. The presence of multiple drug resistance vibrios in emerging inland saline aquaculture systems emphasizes the need for their routine monitoring for developing the risk assessment and mitigation strategies. 相似文献
17.
Vijay Kumar Reddy Surasani Anuj Tyagi Tanaji Kudre 《Journal Of Aquatic Food Product Technology》2017,26(3):356-365
Yield and physicochemical characteristics of protein isolates recovered from rohu processing waste by acid- and alkali-aided protein solubilization methods were studied. pH 2.0 from acidic side and pH 13.0 from alkaline side resulted in maximum protein solubility, whereas minimum solubility was observed at pH 5.5. Except for pH 13.0, theoretical protein recovery was more than the actual recovery after centrifugation. Maximum protein recovery after the first centrifugation cycle was observed for pH 2.0 and pH 13.0, and the maximum protein recovery during the second centrifugation cycle was observed at precipitation pH 5.5 in both acidic and alkaline methods. Acidic and alkaline methods resulted in a total process recovery of 31.81% ± 0.001% and 31.11% ± 0.01%, respectively. Isolates prepared by acidic method had more whiteness and high foaming capacity, whereas isolates prepared using alkaline methods had less whiteness, tough texture, and high foaming stability. No microbial counts were detected in any of the isolates. Acid and alkaline methods described in this study can be used for the recovery of proteins from rohu processing waste with good yields and functionality. 相似文献
18.
Dhananjay Kumar Tewary Anu Bhardwaj Anuj Sharma Arun Kumar Sinha Adarsh Shanker 《Journal of pest science》2006,79(4):209-214
In the light of recent increased interest in developing plant based secondary chemistry into products suitable for integrated pest management, the objective of the present study was to investigate the bioactivity in field simulated conditions and structure–activity relationship of some natural phenylpropenes and their related analogues (1–6) against Aphis craccivora. The activities of different compounds varied depending on the substitution of the functional groups and the side chain attached to the aromatic ring of the phenylpropene. Dimethyleugenol (5) showed maximum activity among all tested phenylpropenes with LC50 values 880 and 2,047 ppm against nymphs and adults respectively. Present study showed that phenylpropenes appear to be promising natural insecticides and may hold potential for identification of new lead structures against A. craccivora. The activity of the most active phenylpropene (5) was comparable with that of the chemical pesticide (dimethoate). 相似文献
19.
Shivachandra SB Yogisharadhya R Ahuja A Bhanuprakash V 《Research in veterinary science》2012,93(3):1128-1131
Pasteurella multocida serogroup B:2, a causative agent of haemorrhagic secpticaemia (HS) in cattle and buffalo especially in tropical regions of Asia and African countries, is known to possess a type IV fimbriae (pili) as one of the virulent factors. In the present study, ptfA gene encoding for type IV fimbrial subunit of P. multocida serogroup B:2 (strain p52), an Indian HS vaccine strain, has been cloned and over-expressed in recombinant Escherichia coli. The recombinant type IV fimbrial subunit protein (～31kDa) including N-terminus histidine tag was purified under denaturing condition and confirmed by western blotting. A homology model of HS causing P. multocida serogroup B:2 fimbrial subunit has also been discussed. The study indicated the potential possibilities to use the recombinant fimbrial protein in developing HS subunit vaccine along with suitable adjuvant. 相似文献