全文获取类型
收费全文 | 1713篇 |
免费 | 150篇 |
专业分类
林业 | 153篇 |
农学 | 81篇 |
基础科学 | 4篇 |
428篇 | |
综合类 | 46篇 |
农作物 | 88篇 |
水产渔业 | 223篇 |
畜牧兽医 | 651篇 |
园艺 | 16篇 |
植物保护 | 173篇 |
出版年
2024年 | 2篇 |
2023年 | 24篇 |
2022年 | 31篇 |
2021年 | 63篇 |
2020年 | 65篇 |
2019年 | 55篇 |
2018年 | 116篇 |
2017年 | 100篇 |
2016年 | 94篇 |
2015年 | 70篇 |
2014年 | 95篇 |
2013年 | 134篇 |
2012年 | 160篇 |
2011年 | 127篇 |
2010年 | 69篇 |
2009年 | 61篇 |
2008年 | 94篇 |
2007年 | 79篇 |
2006年 | 76篇 |
2005年 | 63篇 |
2004年 | 58篇 |
2003年 | 52篇 |
2002年 | 51篇 |
2001年 | 31篇 |
2000年 | 14篇 |
1999年 | 15篇 |
1998年 | 7篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1995年 | 4篇 |
1993年 | 3篇 |
1992年 | 6篇 |
1991年 | 6篇 |
1990年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1983年 | 3篇 |
1982年 | 2篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 3篇 |
1973年 | 4篇 |
1972年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有1863条查询结果,搜索用时 31 毫秒
121.
Perales S Barberá R Lagarda MJ Farré R 《Journal of agricultural and food chemistry》2005,53(9):3721-3726
An adequate calcium intake during the first years of life is needed for normal growth and development and to prevent rickets. The bioavailability of calcium from infant foods (milk-based formulas and fruit juices containing milk and cereals, FMC), the dietary sources of calcium in these stages of life, has been estimated on the basis of simulated gastrointestinal digestion and calcium solubility and dialyzability values and on the efficiency of transport and uptake by Caco-2 cells. The ranking of samples according to calcium bioavailability depends on the use of solubility or dialyzability as criterion. On the basis of the former, the highest value corresponded to adapted formulas and the lowest to fruit juices. However, when using percentage dialysis, the highest value corresponded to fruit juices and the lowest to follow-up formulas. The highest percentages of transport efficiency and uptake by Caco-2 cells corresponded to calcium from the analyzed fruit juices, followed by toddler, follow-up, and adapted formulas. 相似文献
122.
Araújo CL Bezerra IW Oliveira AS Moura FT Macedo LL Gomes CE Barbosa AE Macedo FP Souza TM Franco OL Bloch-J C Sales MP 《Journal of agricultural and food chemistry》2005,53(11):4381-4387
A proteinaceous inhibitor with high activity against trypsin-like serine proteinases was purified from seeds of the tamarind tree (Tamarindus indica) by gel filtration on Shephacryl S-200 followed by a reverse-phase HPLC Vidac C18 TP. The inhibitor, called the tamarind trypsin inhibitor (TTI), showed a Mr of 21.42 kDa by mass spectrometry analysis. TTI was a noncompetitive inhibitor with a Ki value of 1.7 x 10(-9) M. In vitro bioinsecticidal activity against insect digestive enzymes from different orders showed that TTI had remarkable activity against enzymes from coleopteran, Anthonomus grandis (29.6%), Zabrotes subfasciatus (51.6%), Callosobruchus maculatus (86.7%), Rhyzopertha dominica(88.2%), and lepidopteron, Plodia interpuncptella (26.7%), Alabama argillacea (53.8%), and Spodoptera frugiperda (75.5%). Also, digestive enzymes from Diptera, Ceratitis capitata (fruit fly), were inhibited (52.9%). In vivo bioinsecticidal assays toward C. capitata and C. maculatus larvae were developed. The concentration of TTI (w/w) in the artificial seed necessary to cause 50% mortality (LD50) of larvae was 3.6%, and that to reduce mass larvae by 50.0% (ED50) was 3.2%. Furthermore, the mass C. capitata larvae were affected at 53.2% and produced approximately 34% mortality at a level of 4.0% (w/w) of TTI incorporated in artificial diets. 相似文献
123.
124.
125.
Arruda NJ Filho JL Montenegro MC Araújo AN Silva VL 《Journal of agricultural and food chemistry》2003,51(24):6945-6948
This work refers to a very easy to implementate flow injection system with potentiometric detection for l-glutamate determination in food samples. The proposed procedure is based on measurement of carbon dioxide produced by decarboxylation of l-glutamate catalyzed by l-glutamate decarboxylase (E.C. 4.1.1.1.5) from Cucurbita maxima (pumpkin). The FI potentiometric system includes an enzymatic reactor with a length of 8 cm and thickness of 5 mm packed with 200 mg of a C. maxima outer layer cut in to small pieces. The proposed procedure allowed l-glutamate determinations in the concentration interval of 10-100 mmol L(-1) for an injected sample volume of 50 microL. A phosphate buffer (0.1 mol L(-1), pH 5.5) solution flowing at 1.4 mL min(-1) was used as the carrier solution in the system. The results obtained in the analysis of food samples revealed a relative error lower than 5% when compared with those provided by the spectrophometric reference procedure. The immobilized reactor retained its initial activity for 21 days. It was possible to measure 40 samples/h with the flow system proposed. 相似文献
126.
Julio Martin-Mata Raúl Moral Angel Torres-Climent Encarnación Martínez-Sabater Concepción Paredes 《Communications in Soil Science and Plant Analysis》2015,46(4):188-196
The aim of the present article is to show the possibilities of chemometric tools and the parallel factor analysis (PARAFAC) model, as well as to understand the complexities of the fluorescence emission-excitation matrix (EEM) of water-soluble organic matter (WSOM) extracted from sewage sludge samples obtained with different origins and stabilization procedures. The variation in the composition of WSOM in the different sewage sludge samples could be correlated with the conditions of stabilization; therefore, the use of fluorescence spectroscopy to monitor these changes can be a useful tool to optimize the management of this residual flux, avoiding environmental pollution. However, the “contour density” EEM maps obtained with fluorescence present continuous spectra with different peaks, valleys, and shoulders where manual peak picking can be complicated and subjective, especially in complex matrixes such as sewage sludge. With the PARAFAC algorithm, it is possible to resolve the emission and excitation spectra of the different fluorescence components present in the samples and their contribution to the total fluorescence. Also, the contribution (scores) of the different fluorophors can be coupled with another chemometric tool to provide an effective classification method using the stabilization conditions of sewage sludge as main criterion. In this study, a wide survey of sewage sludge samples (287) was characterized by fluorescence emission-excitation matrix. The final molecular composition of the sewage sludge samples was not related to the stabilization (aerobic vs anaerobic) treatments. However, a sewage sludge classification based on a combined spectroscopic-chemometric approach was obtained, which could be used for the optimization of sewage sludge use in agriculture, minimizing the environmental risks. 相似文献
127.
Two different egg based media, with and without the incorporation of sodium pyruvate, were used to isolate M. paratuberculosis from sheep, goat and cattle samples obtained at our laboratory for two years. Both media were adequate for bovine material, with a slightly improved isolation rate for Herrold's egg yolk medium incorporating sodium pyruvate; however, most of the small ruminant strains grew only on L?wenstein-Jensen medium without sodium pyruvate. Those results point out the need to use different media when working with small ruminant samples and provide further evidence for the existence of different varieties of M. paratuberculosis causing paratuberculosis in cattle and in small ruminants. 相似文献
128.
R Nú?ez-Dominguez G E Dickerson L V Cundiff K E Gregory R M Koch 《Journal of animal science》1992,70(8):2328-2337
Experimental lifetime performance data obtained from 156 straightbred Hereford, Angus, and Shorthorn and 172 first-cross heifers were used to estimate heterosis for economic efficiency in a 100-cow herd at age equilibrium under three culling policies and at terminal ages from 6 to 12 yr. All nonpregnant heifers and cows greater than 9 yr of age were culled. The culling policy for removal of nonpregnant cows from second parity through 9 yr of age were 1) no culling, 2) after two consecutive years (actual), and 3) all (imposed). Efficiency was calculated as input cost per unit of output value. A 10-yr average was used for costs of replacement heifers, cow units, and the ratio of calf:cull cow prices (PR), plus higher and lower PR. Input included costs for both cow units and purchased replacements. Output value included both weaned calves and cull cows. Optimum terminal age was mainly a function of PR: 9 yr for average and high PR, but 6 through 9 yr when PR was low, regardless of culling policy or breed groups. Efficiency differences among culling policies were small for high or average PR, but more culling for infertility was beneficial when PR was low. Estimated reductions in unit costs of output value under any culling policy or terminal age were approximately 6% from crossbred cows plus another 6% from crossbred calves, or a total of 12% from specific three-breed crossing of these British breeds. Cost reductions would be somewhat less for rotation crossbreeding but greater for mating smaller crossbred cows with sires of superior growth-carcass breeds. 相似文献
129.
Restriction endonuclease analysis (REA) and plasmid profile were used to study the epidemiology of Pasteurella multocida in a swine pyramid structure. The studied pyramid was comprised of a group of 12 swine farrow-to-finish farms related by unidirectional animal movement. P. multocida isolates were obtained from the lungs of 275 slaughtered pigs. Serotyping was performed by hyaluronidase sensitivity test and toxicity was investigated by the ELISA test. HpaII was used to cleave the P. multocida extracted DNA. REA patterns relationships were studied using the Sokal-Michener coefficients, and the dendrogram was built using the UPGMA system. The 218 P. multocida isolates obtained were distributed in 17 REA patterns. In 9 of the 12 farms studied only 2-3 REA patterns were detected, with one clearly predominant pattern. The 81 strains with plasmids were assigned to six plasmid profiles. REA and plasmid profiles proved to be good epidemiological tools for identifying different strains of P. multocida with the same phenotype. 相似文献
130.