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91.
Within the framework of a molecular exploration of target resistance in populations of Myzus persicae on oilseed rapes in France, (1) the S431F mutation (coding gene ace2), although previously reckoned to be rare, revealed to be frequent, (2) M918L (phenotypically characterised) and L932F (both on para) were found for the first time in M. persicae, and (3) a linkage was revealed between M918L and S431F. While until recently populations developing on French oilseed rapes were dominated by genotypes possessing pyrethroid target resistance and esterase overproduction, to date a different type of dominating genotype, equipped with carbamate and pyrethroid target resistance, seems to be invading such fields.  相似文献   
92.
Ion-pair reverse phase liquid chromatography (LC) and UV detection at 280 nm have been used to determine sodium acifluorfen (sodium-5-[2-chloro-4-(trifluoromethyl)-phenoxy]-2-nitrobenzoate), an experimental diphenyl ether herbicide, in dog feed. Sodium-5-(2,4-dichlorophenoxy)-2-nitrobenzoate is used as the internal standard. The feed is homogenized in 0.01N HCl, followed by ethyl acetate extraction, and centrifugation. The organic layer is removed and evaporated and the residue is reconstituted in methanol and filtered before LC analysis (mobile phase methanol-water (58 + 42), 0.005M in tetrabutylammonium phosphate and 0.045M in (NH4)2HPO4, at pH 7.4). The ion-pair technique offers a high degree of control over the retention characteristics of the herbicide and internal standard. The use of the internal standard permits precise and accurate quantitation and substantially reduces analysis time compared with the external standard method.  相似文献   
93.
94.
Isozyme banding pattern was studied in Guinea grass (Panicum maximum Jacq.), a widely cultivated grass having good fodder value. Similarity among 63 accessions collected from diverse sources was worked out using five enzyme systems (SOD, GOT, ACP, Esterase and Peroxidase) following horizontal starch gel electrophoresis. Biochemical markers such as isozymes are useful supplements in identifying the genetic variation present in any crop. A total of 35 clear and unambiguous bands were used for analysis of which 8 bands were monomorphic. Polymorphism exhibited by 27 bands from all five enzyme systems indicate presence of considerable diversity in this species. The dendrogram generated after UPGMA and SAHN cluster analysis using Jaccard genetic distance showed that 63 accessions from diverse geographical locations could be grouped in three main clusters, of which two could further be divided into sub-clusters. Although the clusters comprised members from different locations, most of the accessions from similar geographical locations tended to cluster in same group.  相似文献   
95.
Well-watered crops having nearly identical ratio of intercept and slope of the “non-water-stressed baseline” would attain their equivalence point temperature at roughly identical air vapor pressure deficit. However, such a behavior of these crops might not prevail as the wet soil dries out progressively. The methods for calculating the equivalence point temperature due to Linacre (1964) and Paw U (1984) gave results which sometimes differed by more than 10 K.  相似文献   
96.
This paper describes a pilot investigation dealing with changes in chemical composition of wood in pollution-stressed white pine trees, taken from two forested ecosystems of Southern Ontario, Canada. The implications of this research lie in a much needed search for a potential bloindicator of a pre-decline, incipient-stress induced (and/or contributed by) the air-pollutants.  相似文献   
97.
Sulphur isotope abundances demonstrate that natural emissions of biogenic H2S and its oxidation products from springs near Paige Mountain, N.W.T., Canada, can be incorporated into surrounding soil and vegetation. δ34S values as low as ? 33‰ for soil and vegetation are the result of dominant uptake of biogenic atmospheric compounds. In contrast, vegetation on a gypsum outcrop remote from the springs, has δ34S values as high as + 26‰ indicating nearly exclusive derivation of S from the soil. Near the springs, soil with vegetative cover is less depleted in 34S than soil lacking cover. Lower needles on a Black Spruce were found to be less depleted in 34S than the upper needles. These observations suggest that upper foliage exerts a canopy effect on lower foliage and in turn, interception by vegetation reduces the flux of atmospheric S compounds to the soil. Clearly, natural emissions of S compounds can interfere in studies of long range transport of industrial emissions; S isotope analyses might identify such interferences and reduce the chance of misinterpretation.  相似文献   
98.
Parkinson's disease (PD) has been proposed to result from the interaction of aging and environment in susceptible individuals. The prevalence of PD in Quebec was 84 per 100 000 in 1985. The regional variation observed has been linked with several environmental factors. We intended to verify the existence of a relation between the development of PD with exposure to 3 metal pollutants: Fe, Mn, and Al. The choice of these metals rests on their potential presence in the environment caused by the industrial activity in the region studied, and on the fact that it theoretically favors the prevalence of the illness. Environmental exposure to these metals and the resulting risks were evaluated using red spruce as a bio-indicator. The metal concentrations in tree rings were determined by neutron activation analysis. These concentrations permitted a temporal estimation of contamination in each of the territories studied, from 1938 to 1987. The risk of environmental exposure was assumed to be proportional to the concentration of the metal. The calculated risks combined with the history of residence allowed a comparison between parkinsonians and controls. It appeared that environmental exposure to the metals was not significantly different between those two groups.  相似文献   
99.
This paper reports the role of microbial biomass in the establishment of N pools in the substratum during primary succession (till 40-year age) in Blastfurnace Slag Dumps, an anthropogenically created land form in the tropics. Initially in the depressions in the slag dumps fine soil particles (silt+clay) accumulate, retaining moisture therein, and providing microsites for the accumulation of microbial biomass. In all sites microbial biomass showed distinct seasonality, with summer-peak and rainy season-low standing crops. During the summer season microbial biomass C ranged from 18.6 μg g−1 in the 1-year old site to ca. 235 μg g−1 in the 40-year old site; correspondingly, microbial biomass N ranged from 1.22 to 40 μg g−1. On sites 2.5-years of age and younger, the microbial biomass N content accounted for more than 50% of the organic N in the soil, whereas the proportion of microbial biomass N was ca. 7% of organic N in 40-year old site. The strong correlation between microbial biomass and total N in soil indicated a significant role of microbes in the build-up of nitrogen during the initial stages of succession in the slag dumps. Though the organic N pool in the soil was low (594 mg kg−1) even after 40 years of succession, the available N (NH4-N and NO3-N) contents in the soil were generally high through the entire age series (ca. 16-32 μg g−1) during the rainy season (which supports active growth of the herbaceous community). The high mineral-N status on the slag dump was related with high N-mineralization rates, particularly in the young sites (20.6 and 13.9 μg g−1 month−1 at 1 and 2.5-year age). We suggest that along with the abiotic factors having strong effect on ecosystem functioning, the microbial biomass, an important biotic factor, shows considerable influence on soil nutrient build-up during early stages of primary succession on the slag dumps. The microbial biomass dynamics initiates biotic control in developing slag dumps ecosystem through its effect on nitrogen pools and availability.  相似文献   
100.
Mud crab larvae, Rhithropanopeus harrisii were exposed to tributyltin oxide, (TBTO) (0.5, 1, 5, 10, 15, or 25 μg l?1) or tributyltin sulfide (TBTS) (0.5, 1, 5, 20, 30, or 50 μg l?1). Differential survival was observed in TBTO concentrations equal to or greater than 10 gg l?1 and in TBTS concentrations equal to or greater than 20 μg l?1 Growth and development rate were assessed as sublethal indices of stress. Of the two, development rate was more sensitive. All groups exposed to either compound had slower development rates than controls. Growth of the larvae, as shown by weight gain, was slightly increased in low exposures (a hormetic response) and decreased significantly in higher concentrations. The mean weight of the 50 μg l?1 TBTS group showed the largest decrease, to 57% of the control. The hormesis response was further examined by measuring daily growth of the zoeae. All groups exposed to tributyltin compounds showed an initial growth lag. The hormesis response occurred in the fourth zoeal stage or megalops. While R. harrisii is relatively tolerant to the acute toxicity of tributyltin compounds compared to other marine crustaceans tested, sublethal responses occur in very low concentrations. This points to the need for analyses to characterize tributyltin environmental concentrations.  相似文献   
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