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921.
A cross-sectional survey of 217 veterinary facilities in Missouri, USA, was conducted to gather information about the occurrence of canine dysautonomia between January 1996 and December 2000. The state was divided into seven geographic regions for sampling, and 40 veterinary facilities from each region were selected randomly by computer-generated numbers to receive the questionnaire. Veterinarians from 74% (95% CI: 69, 79) of clinics in the state that saw canine patients were aware of dysautonomia prior to receiving the survey, and 43 respondents from five of the seven sampled regions stated that dysautonomia had been diagnosed in their practices during the study period. Respondents had diagnosed 182 cases of dysautonomia, leading to an estimated statewide total of 609 (95% CI: 272, 946) cases over the 5 years. Regional incidence estimates ranged from 0 to 1.91 cases per 10,000 dog years at risk. Case-fatality risk was 92%.  相似文献   
922.
This study was conducted on the left Achilles tendon in five clinically normal dogs. The Achilles tendon was surgically exposed and severed 3-4 cm proximal to the point of its insertion. Tenorrhaphy was undertaken by the application of three sutures on the various tendon units of the Achilles tendon using single locking-loop sutures with polyamide no. 1-0. The superficial digital flexor tendon was sutured with catgut using two horizontal mattress sutures. No ultrasound therapy was used in the animals of group I (control). Ultrasound therapy was given to the animals of group II (treated) starting from the third day post-operatively at 0.5 W/cm2 for 10 min daily for 10 days. A cortical screw was used for immobilization of the tibiotarsal joint which was removed 4 weeks after tenorrhaphy. Post-operatively, healing of the Achilles tendon was monitored using clinical observations, ultrasonography, gross and histomorphological observations at various intervals up to 120 days in both groups. Clinically, the dogs showed significant lameness for the first 4-5 days, which disappeared earlier in the ultrasound-treated (group II) animals than the controls (group I). Extension and flexion of the hock joint were found to be near normal at 6 weeks after the repair of the Achilles tendon. Ultrasonography showed anechoic to hypo-echoic echo-texture on days 3 and 7 after repair. By day 40, the echo-texture started to improve to hypo-echoic in group II, but in group I anechoic areas were still observed. However, the tendon showed near normal mottled hypo- to hyper-echoic texture in both groups by day 120. Gross observations suggested that the Achilles tendon in group II showed comparatively fewer adhesions than in group I animals. Histologically, in group II (treated), on day 40, the union was comparatively better without any inflammatory reaction. Bundle formation had begun in the ultrasound-treated animals which was not observed in the control animals. By day 90, more compact parallel bundle formation had taken place with minimum cellularity. Bundle formation was in its advanced stage in the treated animals. By day 120, the tendon tissue was comparatively acellular and looking like a normal tendon. The use of the cortical screw provided good immobilization and ultrasound therapy at 0.5 W/cm2 enhanced the Achilles tendon healing in dogs.  相似文献   
923.
924.
Summary The activity of oxyclozanide was studied in buffalo, cattle, sheep and goats naturally infected withFasciola gigantica. The results indicate a very high efficiency against flukes aged six weeks or more. The drug was also observed to have considerable action on hepatic and ruminal amphistomes. No toxic side-effects were observed in any of the animals treated, except slight softening of faeces with increased frequency of defaecation and inappetance in a few animals. This was observed more frequently in buffalo than in cattle. Sheep and goats were less affected. A transient drop in milk yield for about two to three days was observed in a few animals. No ill effects were observed in experimental animals which were maintained on highly nitrogenous diets. The drug was also well-tolerated by pregnant animals.
Sumario Se estudió la actividad del oxyclozanide en búfalos, bovinos, ovinos y caprinos infectados naturalmente conFasciola gigantica. Los resultados indican una eficiencia muy alta contra Fasciolas de 6 semanas ó mas. La droga tuvo también una acción considerable sobre los amfistomas hepáticos y ruminales. No se observaron efectos colaterales tóxicos en los animales tratados, excepto por el ligero ablandamiento de las heces con un incremento de la frecuencia en la defecación e inapetencia en algunos animales. Esto se observó mas frecuentemente en búfalos que en bovinos. Los ovinos y caprinos fueron menos afectados. Se observó una disminución transitoria en la producción de leche de algunos animales la cual duró de dos a tres dias. No se observaron efectos nocivos en los animales en experimentación que fueron mantenidos con dietas altas en nitrógeno. La droga fué tolerada bastante bien por animales en gestación.

Résumé L'activité de l'oxyclozanide a été étudiée chez les buffles, les bovins, les moutons et les chèvres naturellement infestés parF. gigantica. Les résultats révèlent une trés haute efficacité contre les douves agées de six semaines ou plus. Ce produit se montra aussi très actif sur les amphistomes du foie et du rumen. Aucun effet toxique associé ne fut observé chez les animaux traités, excepté un léger ramollissement des fèces, un accroissement de la fréquence des défécations et de l'inappétence chez un petit nombre d'animaux. Cela fut observé plus souvent chez les buffles que chez les bovins. Chèves et moutons furent moins affectés. On observa chez quelques animaux une baisse temporaire de la production lactée durant deux à trois jours. Aucune réaction défavorable n'apparut chez les animaux d'expérience soumis à régime à forte teneur en azote. Le produit est aussi bien toléré par les femelles en gestation.


Oxyclozanide is a product of Imperial Chemical Industries (trade name ‘Zanil’).  相似文献   
925.
The ability of 25 Pasteurella multocida isolates to adhere in vitro to porcine respiratory tract mucus was examined. Microplate wells were coated with crude mucus preparation, then bacteria were added. After incubation, unbound bacteria were removed by washing, and the number of mucus-bound bacteria was estimated by quantitation of the adherent colony-forming units and by use of an ELISA. Pasteurella multocida had affinity to respiratory tract mucus, although significant differences were not observed in affinity of capsular type-A and type-D isolates. Preliminary characterization, using ultrafiltration, gel filtration chromatography, electrophoresis, and enzymatic treatments, indicated that the receptors may be a class of protein molecules of low molecular weight (less than 25,000). The origin of these receptors, however, is not known at this time.  相似文献   
926.
927.
A simple, rapid technique was developed for preparing chicken-embryo-skin cell cultures utilizing trypsinization of the skin of intact 12-day-old chicken embryos. When cell cultures were inoculated with fowl pox virus, those that consisted of at least 80% epithelial cells yielded a higher virus titer than fibroblast cell cultures.  相似文献   
928.
The pathogenicity of one isolate of Salmonella typhimurium, four isolates of Salmonella heidelberg, three isolates of Salmonella kentucky, two isolates of Salmonella montevideo, one isolate of Salmonella hadar, and two isolates of Salmonella enteritidis (SE), one belonging to phage type (PT)13a and the other to PT34, was investigated in specific-pathogen-free chicks. Three hundred eighty-four chicks were separated into 16 equal groups of 24 chicks. Thirteen groups were inoculated individually with 0.5 ml of broth culture containing 1 x 10(7) colony-forming units (CFU) of either S. typhimurium (one source), S. heidelberg (four sources), S. montevideo (two sources), S. hadar (one source), S. kentucky (three sources), SE PT 13a (one source) or SE PT 34 (one source) by crop gavage. Two groups of 24 chicks were inoculated in the same way with 1 x 10(7) CFU of SE PT4 (chicken-CA) and Salmonella pullorum. Another group of 24 chicks was kept as an uninoculated control group. The chicks were observed daily for clinical signs and mortality. Isolation of salmonella was done from different organs at 7 and 28 days postinoculation (DPI). All the chicks were weighed individually at 7, 14, 21, and 28 DPI. Two chicks chosen at random from each group were euthanatized and necropsied at 7 and 14 DPI and all the remaining live chickens, at 28 DPI. Selected tissues were taken for histopathology at 7 and 14 DPI. Dead chicks were examined for gross lesions and tissues were collected for histopathology. Chicks inoculated with S. pullorum had the highest mortality (66.66%), followed by S. typhimurium (33.33%). Chicks inoculated with S. heidelberg (00-1105-2) and SE PT4 (chicken-CA) had 12.5% mortality and 8.3% mortality, respectively, with SE PT 13a. Ceca were 100% positive for salmonellae at acute or chronic infection compared with other organs. Mean body weight reduction ranged from 0.67% (inoculated with S. kentucky 00-926-2) to 33.23% (inoculated with S. typhimurium 00-372) in the inoculated groups at different weeks compared with uninoculated controls. Gross and microscopic lesions included peritonitis, perihepatitis, yolk sac infection, typhilitis, pneumonia, and enteritis in some groups, especially those inoculated with S. typhimurium, S. heidelberg (00-1 105-2), SE PT4 (chicken-CA), and S. pullorum.  相似文献   
929.
Lacunarity indices as measures of landscape texture   总被引:23,自引:0,他引:23  
Lacunarity analysis is a multi-scaled method of determining the texture associated with patterns of spatial dispersion (i.e., habitat types or species locations) for one-, two-, and three-dimensional data. Lacunarity provides a parsimonious analysis of the overall fraction of a map or transect covered by the attribute of interest, the degree of contagion, the presence of self-similarity, the presence and scale of randomness, and the existence of hierarchical structure. For self-similar patterns, it can be used to determine the fractal dimension. The method is easily implemented on the computer and provides readily interpretable graphic results. Differences in pattern can be detected even among very sparsely occupied maps.  相似文献   
930.
Infection of calves by rotavirus could lead to diarrhea or subclinical disease. Therein, kinetic of viral excretion from a group of asymptomatic calves, previously exposed to a virulent strain of rotavirus, is compared to that of a group the calves of which had diarrhea associated with rotavirus infection. As determined by an enzyme-linked immunosorbent assay (ELISA), duration of rotavirus shedding in feces and maximum yield of virus antigen were similar within the two groups of calves. Rotaviruses isolated from the two above groups of animals were antigenically related as shown by neutralization tests, and showed a similar RNA electrophoretic profile. In conclusion, it is likely that both asymptomatic and diarrheic calves infected by rotavirus are equally a major source of contamination to contact healthy calves.  相似文献   
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