A dot immunobinding assay on nitrocellulose for the detection of bovid herpesvirus-4 antibodies     

K Naeem  S M Goyal 《Journal of veterinary diagnostic investigation》1990,2(2):107-110

A dot immunobinding assay (DIA) was developed for the detection of antibody against bovid herpesvirus-4 (BHV-4) in bovine sera. A semipurified virus preparation was used as the antigen and an antispecies horseradish peroxidase-labeled IgG and diaminobenzidine were employed as the detection system. The sensitivity and specificity of the DIA were similar to those of indirect fluorescent antibody test, indicating the suitability of DIA as a rapid field test for the detection of anti-BHV-4 antibodies in cattle.  相似文献   

Osteopontin gene polymorphism association with milk traits and its expression analysis in milk of riverine buffalo     

Sidra Manzoor  Asif Nadeem  Javed Maryam  Abu Saeed Hashmi  Muhammad Imran  Masroor Ellahi Babar 《Tropical animal health and production》2018,50(2):275-281

Osteopontin gene is regarded as a plausible candidate in mammary gland differentiation and development, expressed by variety of cells, tissues, and biological fluids including milk. The current study was performed in two phases. In the first phase, Osteopontin gene polymorphisms were identified and associated with milk composition such as ash, milk fat, SNF, lactose, and protein. In the second phase, milk samples from five healthy mastitis-free Nili Ravi buffaloes were analyzed for expression of Osteopontin gene at transition (day 15), mid (day 90), and end (day 250) stage of their second lactation. Briefly, blood samples were collected from Nili Ravi buffalo to isolate the genomic DNA, specific primers were designed for PCR amplification. The amplified PCR products were sequenced bi-directionally. Six polymorphisms were identified in the coding region and four in the intronic region of the gene. The results showed that SNP g.38329758 T > C causing substitution of valine to alanine (V127A) was associated with high milk protein. For mRNA expression analysis, somatic cells were separated from milk samples for RNA isolation. Analysis of differential gene expression data has permitted us to illustrate the expression pattern of osteopontin gene in lactating buffalo. The Osteopontin gene was found to be transcribed among all three lactation stages, but expression was observed with the highest value (fold change) in peak lactation and remained elevated till the end of lactation. Identified gene marker may be helpful for the prediction of superior animal for selection. The presented study also gave an insight into the genetic screening and lactation biology of riverine buffalo, offering direction for future research in lactating buffalo.  相似文献   

Germination Ecology of Cenchrus biflorus Roxb.: Effects of Environmental Factors on Seed Germination     

Arslan Masood Peerzada  Muhammad Naeem 《Strength and Conditioning Journal》2018,71(4):424-432

Better understanding related to germination and seedling emergence of plant species assists in predicting the potential distribution and provides insight for efficient management. Cenchrus biflorus Roxb. has been considered as potential forage species in arid environment due to its high nutritive values, prolific seed production, and tolerance to extreme temperature and prolonged drought conditions. A series of laboratory and greenhouse assays were conducted to determine the effect of different environmental factors, such as temperature, light, pH, salinity, osmotic potential, and seed burial depth on the germination and seedling emergence of C. biflorus. The maximum germination (95%) was recorded at 35°C/25°C, followed by 40°C/30°C; however, minimum germination was observed at 45°C/35°C (17.5%). Light significantly promoted the germination with maximum percentage (97.5%) when seeds were exposed to altering light and dark conditions (12/12 h). The osmotic potential for the 50% inhibition of C. biflorus germination was –0.4 MPa, although some seed germinated at –0.8 MPa (12.5%). Germination decreased from 97.5% to 12.5% as salinity stress increased from 0 mM to 200 mM sodium chloride (NaCl) with no germination > 200 mM. Seed germination was significantly affected by pH levels and was between 27.5% and 92.5% at 5–8 pH, respectively. No seedling emerged when seeds were placed on the soil surface; maximum seedling emergence (90%) at 2-cm burial depth and emergence decreased considerably as seeding depth increased above 2 cm. Its tolerance to drought and salinity make C. biflorus a potential candidate to be used as an alternative source during periods of forage scarcity under harsh climatic conditions, and it could possibly be used for rangeland rehabilitation purposes in arid environments.  相似文献   

Henk Bakker: Food security in Africa and Asia, strategies for small-scale agricultural development     

Aakash Goyal  M. Asif 《Agriculture and Human Values》2012,29(4):555-556

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Antibacterial activities of some constituents from oleo-gum-resin of Commiphora mukul     

Saeed MA  Sabir AW 《Fitoterapia》2004,75(2):204-208

The essential oil, chloroform extract and seven sesquiterpenoids compounds newly isolated from the oleo-gum-resin of Commiphora mukul showed a wide range of inhibiting activity against both Gram (+) and Gram (-) bacteria.  相似文献   

The interactive effects of paternal size and offspring feeding strategy on offspring fitness of an Indian major carp Labeo rohita (Hamilton, 1822)     

Md. Moshiur Rahman  Asif Siddique  Md. Ashikur Rahman  Sheikh Mustafizur Rahman  Md. Asaduzzaman  Momotaz Khanom  Mst. Muslima Khatun  Md. Mehedi Hasan 《Aquaculture Research》2020,51(6):2421-2431

Parental condition‐dependent offspring fitness is a well‐discussed issue in animal breeding research, while offspring environment and its interaction with parental condition are currently another very interesting topic to investigate the plasticity of phenotypic traits across an environmental gradient. The present study was, therefore, conducted following a half‐sib breeding design to test the effects of (a) paternal body size, (b) offspring feeding strategy (everyday and every other day) and (c) their interactions on fitness‐related traits (e.g., survival, total length and body area) of offspring of a commercially important aquaculture species, the Indian major carp (Labeo rohita). The analysis revealed that paternal body size had significant effects on offspring survival rate and total length, while no significant influence was found in body area. The study also showed that the offspring feeding strategy significantly affected all offspring traits. Interestingly, the study unveiled the significant interactive effects of paternal condition and offspring feeding for offspring survival rate and total length. These findings suggest that paternal size, offspring rearing environment and their interactions can influence offspring fitness‐related traits which are very essential information for the sustainable production of good quality larvae under variable dietary conditions.  相似文献   

Genetic Variation for Salinity Tolerance in Pakistani Rice (Oryza sativa L.) Germplasm          下载免费PDF全文

A. Sakina  I. Ahmed  A. Shahzad  M. Iqbal  M. Asif 《Journal of Agronomy and Crop Science》2016,202(1):25-36

Soil salinity is one of the major production constraints. Development and planting of salt‐tolerant varieties can reduce yield losses due to salinity. We screened 185 rice genotypes at germination stage in petri dishes under control, 50, 100 and 150 mm salt stress, and at seedling stage in Yoshida's hydroponic nutrient solution under control, 50 and 100 mm salt stress. At germination stage, 15 genotypes including Nona Bokra, Sonahri Kangni, 7421, 7423 and 7467, whereas at seedling stage, 28 genotypes including Nona Bokra, Jajai‐77, KSK‐133, KSK‐282, Fakhr‐e‐Malakand, Pakhal, IR‐6, Khushboo‐95, Shahkar and Shua‐92 were found salt tolerant. Basmati‐370, Mushkan, Homo‐46 and accessions 7436, 7437 and 7720 were sensitive to salinity at both germination and seedling stage. We further screened a subset of 33 salt‐tolerant and salt‐sensitive genotypes with SSR markers. Four SSR markers (RM19, RM171, RM172 and RM189) showed significant association with two or more of the studied traits under 50, 100 and 150 mm salt stress. These markers may be further tested for their potential in marker‐assisted selection. The salt‐tolerant genotypes identified in this study may prove useful in the development of salt‐tolerant rice varieties in adapted genetic background.  相似文献   

H2S as a physiologic vasorelaxant: hypertension in mice with deletion of cystathionine gamma-lyase     

Yang G  Wu L  Jiang B  Yang W  Qi J  Cao K  Meng Q  Mustafa AK  Mu W  Zhang S  Snyder SH  Wang R 《Science (New York, N.Y.)》2008,322(5901):587-590

Studies of nitric oxide over the past two decades have highlighted the fundamental importance of gaseous signaling molecules in biology and medicine. The physiological role of other gases such as carbon monoxide and hydrogen sulfide (H2S) is now receiving increasing attention. Here we show that H2S is physiologically generated by cystathionine gamma-lyase (CSE) and that genetic deletion of this enzyme in mice markedly reduces H2S levels in the serum, heart, aorta, and other tissues. Mutant mice lacking CSE display pronounced hypertension and diminished endothelium-dependent vasorelaxation. CSE is physiologically activated by calcium-calmodulin, which is a mechanism for H2S formation in response to vascular activation. These findings provide direct evidence that H2S is a physiologic vasodilator and regulator of blood pressure.  相似文献   

Polymorphism association and expression analysis of alpha-lactalbumin (LALBA) gene during lactation in Nili Ravi buffalo     

Manzoor  Sidra  Nadeem  Asif  Javed  Maryam 《Tropical animal health and production》2020,52(1):265-271

Tropical Animal Health and Production - Alpha-lactalbumin has been reported as a highly polymorphic gene that potentially alters the gene expression and is associated with milk composition in dairy...  相似文献   

Serum cannabidiol,tetrahydrocannabinol (THC), and their native acid derivatives after transdermal application of a low-THC Cannabis sativa extract in beagles     

Mary Beth Hannon  Kelly A. Deabold  Bryce N. Talsma  Alex Lyubimov  Asif Iqbal  Alexander Zakharov  Lauri Jo Gamble  Joseph J. Wakshlag 《Journal of veterinary pharmacology and therapeutics》2020,43(5):508-511

Cannabinoids hold promise for treating health problems related to inflammation and chronic pain in dogs, in particular cannabidiol (CBD), and its native acid derivative cannabidiolic acid (CBDA). Information regarding systemic delivery of cannabinoids through transdermal routes is sparse. The purpose of this study was to determine pharmacokinetics of transdermal administration of a low-THC Cannabis sativa extract in healthy dogs. Six purpose-bred research beagles were treated with a transdermal CBD-CBDA-rich extract, and serum concentrations of CBD, CBDA, tetrahydrocannabinol (THC), and its acid derivative tetrahydrocannabinolic acid (THCA) were examined prior to and at the end of weeks 1 and 2. A 4 mg/kg dose of total cannabinoids twice daily resulted in appx 10 ng/ml of CBD, 21–32 ng/ml of CBDA, trace amounts of THCA, and unquantifiable amounts of THC in serum at the end of weeks 1 and 2 of treatment. Results showed that CBDA and THCA were absorbed better systemically than CBD or THC.  相似文献