Sequence analysis of the non-structural 3A and 3C protein-coding regions of foot-and-mouth disease virus serotype Asia1 field isolates from an endemic country   总被引：2，自引：0，他引：2  

Biswas S  Sanyal A  Hemadri D  Tosh C  Mohapatra JK  Manoj Kumar R  Bandyopadhyay SK 《Veterinary microbiology》2006,116(1-3):187-193

A total of 18 foot-and-mouth disease virus (FMDV) serotype Asia1 field isolates belonging to two different lineages (including the divergent group) as delineated earlier in VP1-based phylogeny were sequenced in the non-structural 3A and 3C protein-coding regions. The phylogenetic trees representing the regions coding for the non-structural proteins were very similar to that of the structural VP1 protein-coding region. Phylogenetic comparison at 3C region revealed clustering of Asia1 viruses with the isolates of serotypes O, A and C in the previously identified clade. Comparison of amino acid sequences identified lineage-specific signature residues in both the non-structural proteins. Overall analysis of the amino acid substitutions revealed that the 3A coding region was more prone to amino acid alterations than 3C region.  相似文献   

REP-PCR Analysis of Pasteurella multocida Isolates from Wild and Domestic Animals in India     

Saxena MK  Singh VP  Kumar AA  Chaudhuri P  Singh VP  Shivachandra SB  Biswas A  Sharma B 《Veterinary research communications》2006,30(8):851-861

Repetitive extragenic palindromic sequence-based PCR (REP-PCR) was used to characterize 67 field isolates of Pasteurella multocida originating from different animal species and geographical regions of India. REP-PCR was found to be rapid and reproducible (three repeats were done). These isolates yielded different 23 profiles which were clustered into eight groups. The discrimination index was moderate (D value 0.83). Somatic and antigenic typing of the isolates did not reveal any correlation with REP-PCR profiles. There was no host-specific, type-specific, region-specific or pathenogenicity-specific pattern. The REP profiles of isolates obtained from wild animals were similar to those obtained from domestic animals. Two common bands were present in all the isolates irrespective of somatic or antigenic types. The results were not comparable with earlier findings, which had shown high discrimination index and correlation with disease presentation. Saxena, M.K., Singh, V.P., Kumar, A.A., Chaudhuri, P., Singh, V.P., Shivachandra, S.B., Biswas, A. and Sharma, B., 2006. REP–PCR analysis of Pasteurella multocida isolates from wild and domestic animals in India. Veterinary Research Communications, 30(8), 851–861  相似文献   

Landscape contrast: a solution to hidden assumptions in the metacommunity concept?     

Shekhar R. Biswas  Helene H. Wagner 《Landscape Ecology》2012,27(5):621-631

The metacommunity concept provides a spatial perspective on community dynamics, and the landscape provides the physical template for a metacommunity. Several aspects of landscape heterogeneity, such as landscape diversity and composition, and characteristics of the matrix between habitat patches such as habitat connectivity, and geometry of habitat patches, may moderate metacommunity processes. These aspects of landscape heterogeneity are rarely considered explicitly in the metacommunity discussion, however. We propose landscape contrast (i.e., the average dissimilarity in habitat quality between neighboring patches) as a key dimension of landscape heterogeneity. The concept of landscape contrast unifies discrete and continuous landscape representations (homogeneous, gradient, mosaic and binary) and offers a means to integrate landscape heterogeneity in the metacommunity concept. Landscape contrast as perceived by the organisms affects several fundamental metacommunity processes and may thus constrain which metacommunity models may be observed. In a review of empirical metacommunity studies (n = 123), only 22 % of studies were explicit about their underlying landscape model assumptions, with striking differences among taxonomic groups. The assumed landscape model constrained, but did not determine, metacommunity models. Integration and explicit investigation of landscape contrast effects in metacommunity studies are likely to advance ecological theory and facilitate its application to real-world conservation problems.  相似文献   

Comparative efficacy of diminazene diaceturate and diminazene aceturate for the treatment of babesiosis in horses     

Rashid HB  Chaudhry M  Rashid H  Pervez K  Khan MA  Mahmood AK 《Tropical animal health and production》2008,40(6):463-467

The present work was conducted to study the comparative efficacy of two anti-protozoan (babesicidal) drugs on the recovery and health of horses. A total of 80 horses, showing typical clinical symptoms of the disease, were selected for this study; the presence of babesiosis was confirmed through blood smear examination. These animals were divided into two groups i.e. A and B. Horses of Group A, were treated with diminazene diaceturate, while horses of Group B were treated with diminazene aceturate. Efficacy of the drugs was determined by the reversal of clinical signs and a negative blood smear examination. The efficacy of diminazene diaceturate was demonstrated to be 80% while diminazene aceturate was found to have 90% efficacy against babesiosis.  相似文献   

Evaluation of sugar and organic acid composition and their levels in highbush blueberries from two regions of China          下载免费PDF全文

ZHANG Jia  NIE Ji-yun  LI Jing  ZHANG Hui  LI Ye  Saqib FAROOQ  Syed Asim Shah BACHA  WANG Jie 《农业科学学报》2020,19(9):2352-2361

Sweet and sour are the most important taste of blueberries, and they are produced by sugar and acid, respectively. Their contributions to the taste depend not only on the levels of sugar and acid, but also on the types and relative proportions of sugar and acid. Therefore, it is very important to evaluate the composition and levels of sugar and acid in blueberries. Regional differences and variety diversity also affect the sugar and acid characteristics of fruits. Therefore, this study selected two main producing regions in northern China(Weihai and Yingkou) to examine the sugar and acid characteristics of 11 common blueberry cultivars. The indexes measured included soluble sugars, organic acids, soluble solid content and titratable acidity. The results showed that glucose and fructose were the major sugars, and citric acid and quinic acid were the major organic acids. Correlation analysis showed that glucose, fructose, and sucrose were positively correlated with total sugar content; the citric acid content was positively correlated with the titratable acidity and total organic acids. Titratable acidity, glucose, fructose, sucrose, total sugar content, citric acid, shikimic acid and total acid content of the blueberries varied significantly between regions(P0.05). In general, compared with Weihai blueberries, Yingkou blueberries had higher sugar content and lower acid content. The results of this study may provide useful references for the evaluation of sweet and sour flavors and cultivar selection of blueberries.  相似文献   

Disturbance increases negative spatial autocorrelation in species diversity     

Shekhar R. Biswas  Rebecca L. MacDonald  Han Y. H. Chen 《Landscape Ecology》2017,32(4):823-834

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Influence of dietary phosphorus on mineralization and morphology of Indian major carp,catla (<Emphasis Type="Italic">Catla catla</Emphasis>) fingerlings     

Krishna Sukumaran  Asim K. Pal  Narottam P. Sahu  Shrinivas Jahageerdar  Deepak Khambadakone  Dipesh Debnath 《Aquaculture International》2011,19(4):749-763

The present study was conducted to investigate the changes in mineralization and morphology in response to graded levels of dietary phosphorus in Indian major carp, Catla (Catla catla) fingerlings (av. wt. 4.23 ± 0.87 g). The experimental system constituted of 24 150-L capacity plastic tanks (eight treatments, three replicates) stocked with 20 fingerlings each. Eight isonitrogenous and isocaloric semi-purified diets (crude protein 35%, crude lipid 8.5%) were formulated with graded levels of phosphorus (0%-control; 0.1; 0.3; 0.5; 0.7; 0.9; 1.1; 1.3; 1.5%) using KH₂PO₄. The fish were fed twice daily with the respective feeds for a period of 120 days. The percentage of ash content in whole body, vertebrae and opercular bones was found to be significantly (P < 0.05) lower in fish fed low levels of dietary phosphorus (T₁, T₂) in comparison with other treatments. The concentration of phosphorus and calcium was found to be significantly (P < 0.05) lower in whole body, vertebrae and opercula of fish fed low phosphorus diets (T₁, T₂) relative to treatments with higher levels of dietary phosphorus supplementation. The concentration of magnesium in opercular and vertebral tissues of T₁ group was significantly (P < 0.05) lower than all other groups. The X-ray radiographs did not reveal any skeletal deformities in any of the treatments. Truss analysis showed absence of significant morphological variations between fish of different treatments. The deficiency of dietary phosphorus in catla led to significantly lower mineralization of whole body and bone tissues, but the deficiency was not severe enough to cause skeletal deformities and morphological changes in the fish.  相似文献   

The Genome sequence of the SARS-associated coronavirus     

Marra MA  Jones SJ  Astell CR  Holt RA  Brooks-Wilson A  Butterfield YS  Khattra J  Asano JK  Barber SA  Chan SY  Cloutier A  Coughlin SM  Freeman D  Girn N  Griffith OL  Leach SR  Mayo M  McDonald H  Montgomery SB  Pandoh PK  Petrescu AS  Robertson AG  Schein JE  Siddiqui A  Smailus DE  Stott JM  Yang GS  Plummer F  Andonov A  Artsob H  Bastien N  Bernard K  Booth TF  Bowness D  Czub M  Drebot M  Fernando L  Flick R  Garbutt M  Gray M  Grolla A  Jones S  Feldmann H  Meyers A  Kabani A  Li Y  Normand S  Stroher U  Tipples GA  Tyler S  Vogrig R 《Science (New York, N.Y.)》2003,300(5624):1399-1404

We sequenced the 29,751-base genome of the severe acute respiratory syndrome (SARS)-associated coronavirus known as the Tor2 isolate. The genome sequence reveals that this coronavirus is only moderately related to other known coronaviruses, including two human coronaviruses, HCoV-OC43 and HCoV-229E. Phylogenetic analysis of the predicted viral proteins indicates that the virus does not closely resemble any of the three previously known groups of coronaviruses. The genome sequence will aid in the diagnosis of SARS virus infection in humans and potential animal hosts (using polymerase chain reaction and immunological tests), in the development of antivirals (including neutralizing antibodies), and in the identification of putative epitopes for vaccine development.  相似文献   

Effect of post‐methanation effluent on soil physical properties under a soybean‐wheat system in a Vertisol     

Kuntal M. Hati  Ashis K. Biswas  Kalikinkar Bandyopadhyay  Arun K. Misra 《植物养料与土壤学杂志》2004,167(5):584-590

Post‐methanation effluent (PME) generated through bio‐methanation of distillery effluent, a foul‐smelling, dark colored by‐product of distillery industries, is applied to arable land in some areas near the vicinity of the distillery industries as an amendment. The PME contains considerable amount of organic matter and salt besides its high plant‐nutrient content. The present investigation was conducted for three years during 1999–2002 on soybean‐wheat cropping sequence to evaluate the effect of graded levels of post‐methanation effluent (PME) on soil physical properties and crop productivity in a deep Vertisol of central India. Six application doses of PME viz. S_2.5+W₀: 2.5 cm PME applied to soybean and wheat on residual nutrition, S_2.5+W_1.25: 2.5 cm PME to soybean and 1.25 cm to wheat, S_5.0+W₀: 5 cm PME to soybean and wheat on residual nutrition, S_5.0+W_2.5: 5.0 cm PME to soybean and 2.5 cm to wheat, S_10.0+W₀: 10 cm PME to soybean and wheat on residual nutrition, and S_10.0+W_5.0: 10.0 cm PME to soybean and 5.0 cm to wheat, were compared with 100% recommended NPK+FYM ? 4 Mg ha^–1 and control (no fertilizer, manure or PME). The application of PME increased the organic carbon content and electrical conductivity of the soil compared to control and 100% NPK+FYM treatment. The organic C content was maximum in S_10.0+W_5.0 (11.2 g kg^–1) and minimum in control (5.2 g kg^–1). Electrical conductivity increased from 0.47 dS m^–1 in control to 1.58 dS m^–1 in highest dose of PME (S_10.0+W_5.0). The PME treatments have not affected the soil pH. The application of PME showed a significant improvement in the physical properties of the soil. The mean weight diameter (MWD), percent water‐stable aggregation (% WSA), saturated hydraulic conductivity (K_sat), and water retention (WR) at 0.033 MPa suction were significantly (P < 0.05) more while bulk density (BD) and penetration resistance was significantly less in PME‐treated plots than that of control. The MWD showed a linear and positive relationship (r = 0.89**) with the soil organic C. Soybean recorded significantly higher seed yield at all PME treatments than control. Highest average soybean yield (2.39 Mg ha^–1) was recorded in S_10.0+W₀ but yield decreased significantly in S_10.0+W_5.0 (2.08 Mg ha^–1). In wheat, all the PME‐treated plots except S_2.5+W₀ yielded significantly higher than control while the 100% NPK+FYM treatment yielded (3.46 Mg ha^–1) at par with the S_10.0+W_5.0 (4.0 Mg ha^–1) and S_5.0+W_2.5 (3.66 Mg ha^–1). Fresh application of PME to wheat resulted in significant improvement in grain yield over that grown on residual fertility. Thus, application of PME to arable land, as an amendment, could be considered as a viable option for the safe disposal of this industrial waste.  相似文献   

Determinants of adoption behaviour of the fish farmers of Pabda fish culture (Ompok bimaculatus Bloch, 1794) in Tripura,Northeast India     

Shil  Bikash  Lahiri  Biswajit  Pal  Prasenjit  Ghosh  Amitava  Biswas  Pradyut  Singh  Yumlembam Jackie 《Aquaculture International》2022,30(4):2017-2041

Aquaculture International - Most of the adoption studies in catfish culture were restricted to feeding habits, growth rate and morphological studies, neglecting the social factors and adoption of...  相似文献