Resistance to blackrot disease (Xanthomonas campestris (Pam.) Dowson) in cauliflower     

B.R. Sharma  Vishnu Swarup  S.S. Chatterjee 《Scientia Horticulturae》1977,7(1):1-7

Of 200 genotypes of cauliflower screened for their reaction to blackrot disease (Xanthomonas campestris (Pam.) Dowson) ‘MGS-2⊕-3⊕’, ‘Pua Kea’ and ‘SN 445’, derived from Indian cauliflower cultivars, possessed a high degree of resistance. Neither ‘Snowball’ nor its relatives ‘Erfurt’ and ‘Alpha’ showed any resistance. For the plant breeder, soaking the seed in a bacterial suspension is the most useful screening-technique; less useful were the spraying of whole plants with a suspension and the severing of a leaf-tip beneath a suspension. There were positive and highly significant correlations between seed soaking and seedling reaction, seed soaking and adult plant reaction, and seedling reaction and adult plant reaction.  相似文献   

The effect of inoculum size and betamethasone treatment on the course of infection with Dictyocaulus filaria in lambs     

D.N. Dhar  R.L. Sharma  G.C. Bansal 《Veterinary parasitology》1981,8(3):219-228

The effect of Betamethasone treatment on faecal larval production in lambs infected with Dictyocaulus filaria was studied. Four-to-six-month-old male lambs were infected with D. filaria at a dose of 75 larvae per kg body weight (L/kg) and subsequently treated with a total dose of 14 mg of Betamethasone, administered in divided doses, either during the early (day 5–15) or late (day 30–40) stages of infection. Faecal larval yields in treated animals during the period of patency were compared with those of un-treated controls and also with lambs infected with a dose of 150 L/kg.Higher worm establishment, as well as higher percentage survival of infected animals during patency, were associated with infections of 75 L/kg in comparison to 150 L/kg. Treatment with Betamethasone during the early stages of infection resulted in higher worm establishment but similar treatment given during the late stages of infection produced no such effects.The number of larvae per gram of faeces was higher in animals receiving infections of 150 L/kg in comparison to 75 L/kg. Treatment with Betamethasone, whether given during the early or late stages of infection, increased larval output in faeces of infected lambs. However, the output did not reach the levels shown by lambs given infections of 150 L/kg. The total estimated faecal larval yields for the period of patency were higher in the treated animals. The yields were much higher in animals treated during early stages of the infection.  相似文献   

Influence of maternal antibody on efficacy of embryo vaccination with cell-associated and cell-free Marek's disease vaccine   总被引：3，自引：0，他引：3  

J M Sharma  C K Graham 《Avian diseases》1982,26(4):860-870

Vaccination with turkey herpesvirus (HVT) of 18-day-old chicken embryos from a commercial source or from a cross (15 X 7) of two inbred lines induced better protection against early post-hatch challenge with virulent Marek's disease virus (MDV) than vaccination at hatch, despite the presence in embryos of maternally derived antibodies to HVT or to HVT and MDV. However, 50%-protective-dose (PD50) assays revealed that maternal antibodies in embryos reduced vaccine efficacy. The PD50 assays were conducted by vaccinating 15 X 7 embryos with serial dilutions of HVT at the 18th day of incubation. Embryonally vaccinated and unvaccinated chicks were challenged with MDV on the day of hatch. In the absence of maternal antibodies, the PD50 values in plaque-forming units for cell-associated and cell-free HVT were 57 and 328, respectively. In the presence of maternal antibodies, PD50 values for cell-associated and cell-free HVT were 105 and greater than 4,000, respectively.  相似文献   

Studies on some aspects of ovarian abnormalities as a cause of infertility in buffalo cows (bos-bubalis)     

O Sharma  R C Bhalla  B K Soni 《The Indian veterinary journal》1967,44(6):504-508

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Evaluation of a modified-live virus vaccine administered in ovo to protect chickens against Newcastle disease.     

J Ahmad  J M Sharma 《American journal of veterinary research》1992,53(11):1999-2004

The B1 strain of Newcastle disease virus (NDV-B1), which is nonpathogenic for newly hatched chickens, killed embryos when it was used to inoculate chicken eggs at embryonation day 18. Treatment of NDV-B1 with an alkylating agent, ethylmethane sulfonate (EMS) markedly reduced the pathogenicity of the virus for 18-day-old chicken embryos. Eggs inoculated with the modified virus (NDV-B1-EMS) hatched, and the virus was isolated from lungs and spleen of 1-day-old chickens. The hatched chickens developed antibody to NDV and were protected against challenge exposure (at 4 weeks of age) with a highly virulent GB-Texas strain of NDV. Presence of maternal antibody to NDV in embryonating eggs did not influence the protective ability of NDV-B1-EMS, which also induced protective immunity when administered to 4-week-old chickens. The 50% protective dose of NDV-B1-EMS in maternal antibody-negative and -positive embryos was calculated to be 10.77 and 17.70 embryo 50% lethal doses, respectively. Results of the study indicated that NDV-B1-EMS may be used as an embryo vaccine to protect chickens against Newcastle disease.  相似文献   

Biochemical variations in endometrium during oestrous cycle in buffalo (Bubalus bubalis)     

N S Bugalia  R D Sharma  R K Biswas 《Archiv fuer experimentelle veterinaermedizin》1988,42(5):714-717

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Detection of goat pox antigen and antibody by the counter immunoelectrophoresis test     

Bhaskar Sharma  B. S. Negi  A. B. Pandey  S. K. Bandyopadhyay  H. Shankar  M. P. Yadav 《Tropical animal health and production》1988,20(2):109-113

Summary The counterimmunoelectrophoresis (CIE) test was standardised for the detection of goat pox antigen and antibody using inactivated antigens. The chloroform inactivated and live antigens were equally sensitive for detection of goat pox precipitins. The precipitinogens of goat pox virus (GPV) were found to be soluble in nature. The CIE test was quick as well as more sensitive than the agar gel precipitation test for detection of GPV antibody/antigen. The CIE employing inactivated antigen has been used for the first time in the detection of GPV antibodies/antigens.

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Deteccion Del Antigeno Y Anticuerpos De Viruela Caprina Mediate La Prueba De Contrainmunoelectroforesis

Resumen Se estandarizó la prueba de contrainmunoelectroforesis, para la detección del antígeno y anticuerpos del virus de la viruela caprina, usando antígenos inactivados. El antígeno inactivado con cloroformo y el antígeno vivo, fueron igualmente sensitivos para la detección de precipitinas de viruela caprina. Los precipitógenos del virus de la viruela caprina se encontraron que eran solubles. La prueba de contrainmunoelectroforesis fue más rápida y más sensitiva que la precipitación agar gelatina para la detección de anticuerpos/antígenos del virus de la viruela caprina. La prueba de contrainmunoelectroforesis con antígeno inactivado ha sido utilizada por vez primera en la detección de anticuerpos/antígenos del virus de la viruela caprina.

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Detection De l'Antigene Et De l'Anticorps Variole Caprine Par Un Test De Contrimmuno-Electrophorese

Résumé Le test de contrimmuno-électrophorèse (CIE) a été standardisé pour la détection de l'antigène et de l'anticorps variole caprine avec des antigènes inactivés. Les antigènes vivants et inactivés par le chloroforme sont de sensibilité équivalente pour la détection des précipitines variole caprine. On a montré que ces précipitogènes du virus variole caprine (VVC) étaient de nature soluble. Le CIE est rapide et plus sensible que le test de précipitation en gélose pour la détection des antigènes et anticorps VVC. C'est la première fois que le CIE mettant en oeuvre un antigène inactivé a été utilisé.

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Effects of allo-immunization of goat bucks with goat-testes homogenates on spermatogenesis sperm motility and production of cross reacting antibodies.     

C P Sharma  R Saini  G S Bilaspuri  S P Ahuja 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1979,26(6):433-439

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Elongation Rate of Wheat Root Axes     

R. B. Sharma  B. P. Ghildyal 《Journal of Agronomy and Crop Science》1987,159(5):329-334

The elongation rate of seminal root axes for two wheat ( Triticum aestivum L.) genotypes (one gene dwarf Sonalika RR-21 and three gene dwarf UP 301 ) grown in pots under three fluctuating soil water tension regimes of 0.3 to 0.8, 2.0 to 2.5 and 4.0 to 4.5 bars was measured throughout the growing period.
Two distinct peaks were observed for both genotypes. The first peak of highest elongation rate (25.0 to 35.5 mm day⁻¹) for both genotypes occurred during 22–28 days after planting which corresponds to the crown root initiation stage of the crop. The second lower peak (11.2 to 14.5 mm day⁻¹) occurred during 49–60 days after planting for Sonalika RR-21 but 60–72 days for UP 301. The time of occurrence of second peak corresponds to the stem elongation stage.
The elongation rate of root axes differed significantly for different stages and genotypes but when considered over the whole growing season, soil water tension regimes showed no effect. Over a period of 72 days from planting, average elongation rate ranged from 11.3 to 12.7 mm day⁻¹. The elongation rate of root axes bore no consistent relationship with either mean air or soil temperature at 5 and 15 cm depths that prevailed during crop growth period.  相似文献   

Prevalence ofToxoplasma antibodies in sheep and goats in the area of Hissar,Haryana, India     

S. P. Sharma  O. P. Gautam 《Tropical animal health and production》1972,4(4):245-248

Summary An endeavour was made to assess the prevalence of Toxoplasma infection in sheep and goats in Hissar using the indirect haemagglutination test. The incidence in sheep was 6·6 per cent and in goats 11·5 per cent and was higher in the female than in the male in both species.

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Sumario Se ha intentado evaluar la prevalencia de infecciones a Toxoplasma en ovinos y caprinos en Hissar mediante el uso de la prueba indirecta de hemaglutinacion. La incidencia en ovinos fue de 6.6 por ciento y en caprinos de 11.5 por ciento y era mas alta en las hembras que en los machos en ambas especies.

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Résumé Dans une tentative pour établir la prévalence de l'infection à toxoplasmes chez le mouton et la chèvre dans la région de Hissar, on a utilisé l'épreuve d'hémagglutination indirecte. Chez le mouton, l'incidence a été de 6, 6 p. 100 et de 11, 5 p. 100 chez la shévre, toujours plus élevée chez les femelles que chez les males dans les deux espèces.

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