IBDV-induced bursal T lymphocytes inhibit mitogenic response of normal splenocytes   总被引：6，自引：0，他引：6  

Kim IJ  Sharma JM 《Veterinary immunology and immunopathology》2000,74(1-2):47-57

We examined the suppressive activity of bursal T cells induced by infectious bursal disease virus (IBDV) in inbred (15x7) and outbred commercial specific-pathogen-free (SPF) chickens. The suppressive activity was measured by the ability of bursal and splenic T cells from IBDV-infected chickens to inhibit mitogenic responses of normal splenocytes. The bursacytes but not the splenocytes of IBDV-infected chickens inhibited the mitogenic responses of normal splenocytes. The mitogenic inhibition by the bursacytes of IBDV-infected chickens was dose-dependent. The suppression was observed both in inbred and non-inbred chickens, and thus, was non MHC-restricted. Cell-sorting experiments revealed that both CD4(+) and CD8(+) cells from the bursa of IBDV-infected chickens, as well as cell-culture supernatants conditioned by these cells, mediated suppression. Suppressor T (Ts) cells may therefore be involved in the immunosuppression induced by IBDV.  相似文献   

Subcutaneous pharmacokinetics and dosage regimen of cefotaxime in buffalo calves (Bubalus bubalis)     

Suresh Kumar Sharma  Anil Kumar Srivastava 《Journal of veterinary science (Suw?n-si, Korea)》2006,7(2):119-122

The pharmacokinetics and dosage regimen of cefotaxime following its single subcutaneous administration (10 mg/kg) were investigated in buffalo calves. Plasma and urine samples were collected over 10 and 24 h post administration, respectively. Cefotaxime in plasma and urine was estimated by microbiological assay technique using E. coli as test organism. The pharmacokinetic profiles fitted one-compartment open model. The peak plasma levels of cefotaxime were 6.48 ± 0.52 µg/ml at 30 min and the drug was detected upto 10 h. The absorption half-life and elimination half-life were 0.173 ± 0.033 h and 1.77 ± 0.02 h, respectively. The apparent volume of distribution and total body clearance were 1.17 ± 0.10 l/kg and 0.45 ± 0.03 l/kg/h, respectively. The urinary excretion of cefotaxime in 24 h, was 5.36 ± 1.19 percent of total administrated dose. A satisfactory subcutaneous dosage regimen for cefotaxime in buffalo calves would be 13 mg/kg repeated at 12 h intervals.  相似文献   

Hybridization and in vitro culture of an orchid hybrid Ascocenda ‘Kangla’     

Rajkumar Kishor  P.S. Sha Valli Khan  G.J. Sharma 《Scientia Horticulturae》2006

The present study focuses on hybridization program involving two species belonging to two different vandaceous genera, viz., Ascocentrum ampullaceum (Roxb.) Schltr. var. auranticum, a narrow endemic orchid of Manipur and Vanda coerulea Griff., an endangered orchid of Appendix I of CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora), to synthesize the primary hybrid genus with intermediate and improved characters in the F₁ generation. Observations on the crossability in the present bigeneric cross between V. coerulea and A. auranticum had been achieved with 60% success when V. coerulea was taken as female parent. Murashige and Skoog (MS) basal medium at half-strength was effective for the development of the hybrid seedlings of V. coerulea × A. auranticum followed by Vacin and Went (VW) and Knudson C (KC) media. The best response of seedling growth was observed on MS medium at half-strength supplemented with 2.3 μM kinetin + 0.5 μM α-naphthalene acetic acid with maximum shoot height (2.7 cm), leaf number (4.6) and root number (4.1) after 150 days of inoculation. The survival percentage and growth performance of the seedlings were found to be higher (80% survival) in potting substrate consisting of brick:charcoal in the ratio 2:1 mulched with moss (Sphagnum sp.) than in potting substrate consisting of brick:charcoal:tree fern in the ratio 2:1:1. The first flowering was observed in the hybrid seedlings of V. coerulea × A. auranticum after 2 years of transfer to the ex vitro environment. Morphologically, the flowers differed from that of the parents clearly showing the success of the hybridization experiment. Registration of the hybrid has been made with the Royal Horticultural Society with the nomenclature Ascocenda ‘Kangla’ (No. T128725).  相似文献   

Relationship between light,fruit and leaf mineral content with albinism incidence in strawberry (Fragaria x ananassa Duch.)     

R.R. Sharma  V.B. PatelHare Krishna 《Scientia Horticulturae》2006

A study was conducted in strawberry to establish a relationship between shading, mineral nutrient of leaves and fruits with albinism incidence. Plants grown under shade produced albino fruits in higher proportion than those grown in open fields. Similarly, plants under shade produced smaller sized fruits and have lower fruit yield. Among cultivars, Etna had highest incidence of albinism (49.6%) and Sweet Charlie the lowest (14.4%). Dry matter content (%), concentration of five major nutrients, viz. N, P, K, Ca, Mg, S and N:Ca and K:Ca nutrient ratios did not differ significantly in the leaves of plants producing normal or albino fruits. However, in contrast, the concentration of K was notably higher (1.97 mg g⁻¹ fresh tissue weight) and that of Ca was lower (0.098 mg g⁻¹ fresh tissue weight) in albino fruits than normal ones. Consequently, the ratios of N:Ca (11.34) and K:Ca (20.08) were higher in albino fruits than normal ones. Cultivars also differed widely in respect to dry matter (%), mineral content and nutrient ratios. Thus, it appears that lower light intensity favours the development of albinism in strawberry, and it seems that calcium is not the basic cause of albinism, but increased vigour associated with overuse of N and K might be positively associated with it.  相似文献   

Bioinformatically mined simple sequence repeats in UniGene of Citrus sinensis     

Asheesh Shanker  Aarti BhargavaRicha Bajpai  Stuti SinghSucharita Srivastava  Vinay Sharma 《Scientia Horticulturae》2007

Characterization of microsatellites is extremely important for the development of molecular markers. Here, we present the detection and abundance of microsatellites or simple sequence repeats (SSRs) in UniGene sequences of Citrus sinensis. A total of 427 SSRs were mined in 8786 UniGene sequences downloaded from National Center for Biotechnology Information (NCBI). Depending on the repeat units, the length of SSRs ranged from 14 to 21 for mono-, 14 to 48 for di-, 18 to 48 for tri-, 24 to 40 for tetra- and 42 bp for hexa-nucleotide repeats. Average density of SSRs (1SSR/12.92 kb of 5518.71 kb sequences mined) suggests that only 4.43% of sequences contained SSRs. Di-nucleotide repeats were most frequent repeat type (49.41%) followed by tri-nucleotide repeats (41.45%). An attempt was made to design primer pairs for 427 identified SSRs but these were found only for 216 sequences. The positions of SSRs with respect to open reading frame (ORF) detected and annotation of sequences containing SSRs were also carried out to assign function to each of the sequences.  相似文献   

An outbreak of gizzard erosion and ulceration in chicks in Zambia     

R N Sharma  G S Pandey 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1990,43(3):301-304

An outbreak of gizzard erosion and ulceration in broilers in Zambia is described. Approximately 100,000 chickens in the age group of 2-9 weeks were affected. Mortality ranged from 5 to 30%. In a majority of cases, crops, oesophaguses and gizzards were engorged with dark brown to black watery material. Lesions were mainly in the gizzard comprising erosions and ulcers. High levels of fish meal in the ration is indicated as a causative factor.  相似文献   

Reclamation of saline‐sodic soil under a rice–wheat system by horizontal surface flushing     

A. K. Nayak  D. K. Sharma  V. K. Mishra  P. S. Minhas  C. L. Verma 《Soil Use and Management》2008,24(4):337-343

Saline sodic soil with a high content of soluble carbonates is one of the important agricultural soils on the Central Indo‐Gangetic plains and elsewhere. Conventional reclamation procedures using gypsum application followed by vertical leaching (GC) is uneconomic; high ECe and precipitation of applied gypsum, reacting with soluble carbonates, reduce the efficacy of gypsum in these soils. This paper reports results from a project designed to evaluate reclamation by irrigation of the ploughed soil and turning of soil with a power tiller followed by flushing of standing water after 24 h, a second flushing after 7 days and subsequent application of gypsum and vertical leaching (GF2). Average rice and wheat production after GF2 significantly increased (25 and 62%, respectively) over the conventional practice. Compared with conventional treatment, GF2 significantly reduced the ECe and SAR of the soil and improved physical properties such as ζ‐potential, dispersible clay content, water stable aggregates expressed as MWD, and saturated hydraulic conductivity. Split application of gypsum between flushing (GF1/2 and GF2/3) gave similar results to GF2 in terms of soil amelioration and crop production.  相似文献   

Urinary incontinence in a bitch caused by vaginal fibroma     

P N Sahay  L L Dass  A A Khan  K K Singh 《The Veterinary record》1985,116(3):76-77

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Effect of Gypsum Application on Long Term Changes in Soil Properties and Crop Growth in Sodic Soils under Field Conditions     

D. P. Sharma 《Journal of Agronomy and Crop Science》1986,156(3):166-172

In a field study, conducted on farmer's waste sodic soils, Aquic Natrustalf, 12.5 t/ha gypsum was surface mixed. Effect of gypsum application on soil properties and crop yields of rice and wheat grown in succession was evaluated after one to five years of gypsum application. The results showed that soil pH, electrical conductivity, calcium carbonate and soil dispersion decreased, whereas organic carbon, hydraulic conductivity, water infiltration and storage increased considerably after five years of gypsum application. In the initial years the improvement in soil properties was more in the surface layers and extended to lower layers slowly in the following years. Average grain yields of rice and wheat in the first year were 4.2 and 1.8 t/ha, respectively. The yields further increased to 6.1 and 2.5 t/ha in the 5th year, but the yields were lower than the yields obtained in research experiment. Relationships between wheat grain yield and pH of 0–15 and 15–30 cm layers were established. Wheat yield reduced by 45 percent when pH of 0–15 cm layer increased from 9.0 to 9.5. The sodicity of sub soil was still too high to permit the cultivation of deep rooted crops even after five years of gypsum application. Normal crop production is possible in these sodic soils, given sufficient time to reduce the sub soil sodicity.  相似文献   

Effect of bronchodilator and intravascular oxygen releaser on the course of Dictyocaulus filaria infection in lambs     

R L Sharma  T K Bhat 《Veterinary parasitology》1990,35(1-2):147-155

The effect of a bronchodilator (or ciprenaline sulphate) and intravascular oxygen releaser (sodium dihydrogen orthophosphate) on the host in experimental Dictyocaulus filaria infection was studied. Fifteen male lambs of Dorset-Muzaffarnagri breed, aged 4-6 months, were divided into four groups of four (infected bronchodilator), four (infected i.v. O2 releaser), four (infected untreated controls) and three (uninfected controls). The administration of i.v. O2 releaser helped in increasing the length of useful patency, estimated total larval production and survival rate of D. filaria producer lambs. The administration of I.V. O2 releaser and bronchodilator helped in efficiently restoring the altered values of blood pH, haemoglobin, packed cell volume, erythrocyte sedimentation rate and osmotic fragility of erythrocytes to near normal levels. However, the blood clotting time and level of lactate dehydrogenase activity remained altered and followed a course typical of ovine dictyocauliosis.  相似文献