Brassica juncea (L.) Czern & Coss is widely grown as an oilseed crop in the Indian subcontinent. White rust disease caused by Albugo candida (Pers.) Kuntze is a serious disease of this crop causing considerable yield loss every year. The present study was undertaken to identify molecular markers for the locus controlling white rust resistance in a mustard accession, BEC‐144, using a set of 94 recombinant inbred lines (RILs). The screening of individual RILs using an isolate highly virulent on the popular Indian cultivar ‘Varuna’ revealed the presence of a major locus for rust resistance in BEC‐144. Based on screening of 186 decamer primers employing bulked segregant analysis (BSA), 11 random amplified polymorphic DNA markers were identified, which distinguished the parental lines and the bulks. Five of these markers showed linkage with the rust resistance locus. Two markers, OPN0l000 and OPB061000, were linked in coupling and repulsion phases at 9.9 cM and 5.5 cM, respectively, on either side of the locus. The presence of only two double recombinants in a population of 94 RILs suggested that the simultaneous use of both markers would ensure efficient transfer of the target gene in mustard breeding programmes. 相似文献
The genetic bases of leaf rust resistance in wheat (Triticum aestivum L.) line CSP44, selected from the Australian cultivar Condor, and Indian cultivar VL404, were studied. The reaction patterns
of CSP44 and VL404 against Indian races 12, 77, 77-1, 77-2, 77-3, 77-4, 77-5 and 108 were different from reaction patterns
shown by near-isogenic lines with known adult plant resistance (APR) genes, viz. Lr12, Lr13, Lr22b and Lr34. Although the reaction patterns of CSP44 and VL404 were similar to the near-isogenic line Tc+Lr22a, tests of allelism indicated absence of Lr22a in both CSP44 and VL404. On the basis of genetic studies, their resistances in field tests against race 77-5, the most virulent
race from the Indian sub-continent, were each ascribed to two genes. One of the two genes in each wheat was identified to
be the non-hypersensitive APR gene Lr34. The second APR genes in CSP44 and VL404 gave hypersensitive reaction types and were recessive and dominant, respectively.
The gene in CSP44 was designated Lr48and the gene in VL404, Lr49.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
Integrated effects of fly ash (FA), farmyard manure (FYM), and chemical fertilizers recommended dose of fertilizer (RDF) on the performance of rice (Oryza sativa L.) and their residual effect on rapeseed (Brassica camprestris L. vartoria) were studied for two years in loamy acidic inceptisol of Assam, India.
The FA was generated from a bituminous and lignite coal-based captive thermal power plant of Hindustan Paper Corporation Ltd (HPCL), Assam. Rice yields were higher when FA, FYM, and RDF were used collectively vis-à-vis sole application of RDF reflected also in rapeseed under residual effect . FA effect on mean rice equivalent yield of the rice–rapeseed system was the highest (24.4%) under integrated application. Nickel (Ni) and cadmium (Cd) contents in rice decreased with increasing FA, while arsenic (As) was just the opposite. Integration of FA, FYM, and RDF was effective in lowering Ni, Cd, and As in rice compared to 100% RDF alone or together with FA. The residual effect on rapeseed was similar for Ni and As while the Cd content increased. Blending of FA, FYM, and RDF also positively altered the residual soil pH, organic carbon (C), and nitrogen, phosphorus, and potassium (NPK). Thus, FA can be an integral component of integrated plant nutrition system (IPNS) in augmenting crop yield and residual benefits in loamy acid inceptisol. 相似文献
Pyrolysis of tobacco was studied in oxidative and nonoxidative (inert) environments at atmospheric pressure and temperatures ranging from 150 to 750 degrees C. The objective was to study the effect of pyrolysis conditions on the characteristics of the solid residue, i.e., char. The char was characterized using cross-polarization (13)C nuclear magnetic resonance (CPMAS NMR), Fourier transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), the Brunauer-Emmett-Teller (BET) surface area, and the elemental composition. The char yield from pyrolysis (i.e., nonoxidative) decreased sharply with an increase in temperature to ca. 22% (dry, ash-free basis) at high temperatures. In oxidative pyrolysis, i.e., in 5% oxygen, the char was completely oxidized above 600 degrees C. The gaseous product from pyrolysis at high temperatures contained a significant concentration of hydrogen. The surface area of the char was low, with a maximum of 8 m(2)/g at 400 degrees C. SEM analysis indicated that pyrolysis of the tobacco led to a gradual accumulation of inorganic crystals on the exposed surfaces, and some constituents also melted, resulting in the formation of vesicles by evolving gas. NMR analysis showed significant changes in pectin and sugar constituents of the tobacco and breaking of glycosidic bonds of cellulose at 300-500 degrees C before the char became predominantly aromatic at high temperatures. FTIR results showed a continuous decrease in the intensity of the OH stretch with temperature and the aromatic character to be at maximum at 550-650 degrees C. The H/C ratio of the char decreased continuously with temperature, while the O/C ratio became constant above 300 degrees C due to the presence of oxides and carbonates in the char. The results are consistent with the analysis of the evolved gases. 相似文献
BACKGROUND: The insulin-like growth factor (IGF) system is implicated in the pathogenesis of diabetes and cardiovascular disease. OBJECTIVE: We report the effects of total energy intake on the IGF system in two populations with markedly different dietary macronutrient intake and cardiovascular event rate. DESIGN, SUBJECTS AND SETTING: Dietary macronutrient intake was measured in a specific Gujarati migrant community in Sandwell, UK (n=205) compared with people still resident in the same villages of origin in India (n=246). Fasting IGF-I, IGF-binding protein (IGFBP)-1 and IGFBP-3, insulin and glucose (0 and 2-hour) were measured. RESULTS: Total energy and total fat intake were higher in UK migrants, as were IGFBP-3 and IGF-I (mean (95% confidence interval): 145.9 (138.1-153.6) vs. 100.9 (94.6-107.3) ng ml(-1); F=76.6, P<0.001). IGFBP-1 was lower in UK migrants (29.5 (25.9-33.0) vs. 56.5 (50.6-62.5) microg l(-1); F=48.4, P<0.001). At both sites, IGF-I correlated positively with total energy (Spearman's rho=0.45, P<0.001) and total fat (rho=0.44, P<0.001) as did IGFBP-3 with total energy (rho=0.21, P<0.05) and fat (rho=0.26, P<0.001). Conversely, in Indian Gujaratis, IGFBP-1 fell with increasing total energy (rho=-0.27, P<0.001) and fat intake (rho=-0.26, P<0.01) but not in UK Gujaratis. Multiple linear regression modelling showed that increasing quartiles of fat intake were associated with higher IGF-I (beta=0.42, P=0.007) independent of age, body mass index, plasma insulin, fatty acids and 2-hour glucose. CONCLUSION: In these genetically similar groups, migration to the UK and adoption of a different diet is associated with marked changes in the IGF system, suggesting that environmental factors profoundly modulate serum concentrations and actions of IGFs. 相似文献
Irrigated green forage production under reduced tillage is thwarted by the failure to obtain adequate N fertilization. Forage accumulation, yield and nitrogen response in relation to tillage and N application were studied for two years during 1980–1982. Forage accumulation, plant height and final forage yield were significantly affected under minimum tillage (T1). However, plant growth and green and dry forage yields were comparable under reduced (T2) and conventional (T3) tillage. Reduction in yield under T1 was associated with restricted root growth and high soil strength and bulk density in the surface and subsurface layers. The optimum dose of N for T1, T2 and T3 was calculated to be 107, 120 and 113 kg N ha?1 and the estimated dry matter yield for these rates of N under each of the three tillage systems was 6.6, 9.1 and 9.5 t ha?1, respectively. The interaction between different tillage systems and N application rates for green and dry matter production was not significant. 相似文献
Pigeonpea [Cajanus cajan (L.) Millsp.] is an important legume crop in South Asia, East and southern Africa, and the Caribbean. Pod fly (Melanagromyza obtusa Malloch) and pod wasp (Tanaostigmodes cajaninae La Salle) are important constraints to increase the production and productivity of pigeonpea under subsistence farming conditions. Host plant-resistance can be used as an important component for the management of these pests, and therefore, we evaluated 28 accessions of wild relatives of pigeonpea for resistance to these pests. There were significant inter- and intra-species differences in the relative susceptibility to pod fly and pod wasp damage. Accessions belonging to Cajanus scarabaeoides (L.) Thouars, C. sericeus (Benth. ex Bak.) van der Maesen, Rhynchosia bracteata Benth. ex Bak., C. acutifolius (F.v. Muell.) van der Maesen, C. lineatus (W. & A.) van der Maesen, and C. albicans (W. & A.) van der Maesen showed resistance to pod fly damage, while those from C. platycarpus (Benth.) van der Maesen, C. cajanifolius (Haines) van der Maesen and R. aurea DC. were susceptible. For the pod wasp, some of the accessions from C. scarabaeoides, C. albicans, Flemingia stricta Roxb., and R. bracteata (Roxb.) Wight showed a resistant reaction, while ICPW 83 belonging to C. scarabaeoides showed a susceptible reaction. ICPW 141, ICPW 278, and ICPW 280 (C. scarabaeoides), ICPW 214 (R. bracteata), ICPW 14 (C. albicans), and ICPW 202 (F. stricta) showed resistance to both pod fly and pod wasp damage. There was considerable variation in accessions belonging to different species for their susceptibility to pod fly and pod wasp, which can be exploited to breed for resistance to these pests. There was a negative association between pod wasp and pod borer damage, and therefore, it is important to keep track of the relative susceptibility of pigeonpea genotypes to pod wasp, while breeding for resistance to pod borers. 相似文献
The present study was undertaken in five major forest types (dominated by Quercus semecarpifolia, Quercus floribunda, Acer acuminatum, Abies pindrow and Aesculus indica, respectively) between 2400 and 2850 m a.s.l. in a moist temperate forest of the Mandal-Chopta area in the Garhwal region of Uttarakhand, India. The aim was to assess the variation in composition and diversity in different vegetation layers, i.e., herb, shrub and tree, among these five forest types. Diversity indices, such as the Shannon-Wiener diversity index, density, total basal cover, Simpson’s concentration of dominance index, Simpson’s diversity index, Pielou’s equitability, species richness, species heterogeneity and β-diversity, were calculated to understand community structures. Dominance-diversity curves were drawn to ascertain resource apportionment among various species in different forest types. 相似文献
The legume pod borer, Helicoverpa armigera (Hübn.), is one of the major constraints to chickpea production, and host plant resistance is an important component for
the management of this pest. The levels of resistance in the cultivated chickpea are low to moderate, and therefore, we evaluated
17 accessions of perennial Cicer along with three cultivated chickpea genotypes for resistance to H. armigera. There was a significant reduction in both leaf feeding and larval weights when the larvae were fed on the leaves of Cicer microphyllum Benth. accessions ICC 17146, ICC 17236, ICC 17240, and ICC 17248. Relative resistance index based on leaf feeding, larval
survival, and larval weight indicated that C. microphyllum accessions ICC 17146, ICC 17236, ICC 17234, ICC 17240, ICC 17243, and ICC 17248 were highly resistant to H. armigera. Under natural infestation, accessions belonging to C. microphyllum, C. canariense Santos Guerra et Lewis, and C. macracanthum M. Pop suffered a damage rating of <2.0 compared to 4.0 in C. judaicum Boiss. accession ICC 17148 (annual species) and 8.5–9.0 in the cultivated chickpeas (1 = <10% leaf area damaged, and, 9 = >80%
leaf area damaged). There was considerable diversity in the accessions belonging to perennial wild species of chickpea, and
these can be exploited to increase the levels and diversify the basis of resistance to H. armigera in the cultivated chickpea. 相似文献
Abstract Efficient nutrient and water use are two important considerations to obtain good harvests of wheat. This necessitates the development of an effective nutrient management technique that not only increases yield, but simultaneously can save nutrient and water use. In this context, a field experiment was conducted at Indian Agricultural Research Institute, New Delhi, India to evaluate the residual effect of sesbania and rice bean (in-situ), subabul (ex-situ) green manuring and Zinc (Zn) fertilization, using chelated Zn-ethylenediaminetetraacetic acid (Zn-EDTA) on nutrient use, yields and water productivity of wheat under rice–wheat cropping system. Among residual effects of green manure crops and Zn fertilization, sesbania and foliar spray of 0.5% chelated Zn-EDTA at 20, 40, 60 and 80 days after sowing (DAS) recorded significantly higher nutrient content and uptake and yields than other green manure crops and Zn treatments. Residual effect of sesbania saved about 46.5?×?103 and 30.5?×?103 L irrigation water per tonne of wheat over subabul and rice bean, respectively. Foliar spray of 0.5% chelated Zn-EDTA at 20, 40, 60 and 80 DAS saved about 55.5?×?103, 47?×?103 and 13?×?103 L irrigation water per tonne wheat over residual effect of 5?kg Zn ha?1 through chelated Zn-EDTA as soil application, 2.5?kg Zn ha?1 through chelated Zn-EDTA as soil application + 1 foliar spray of 0.5% chelated Zn-EDTA at flowering and foliar spray of 0.5% chelated Zn-EDTA at active tillering?+?flowering?+?grain filling, respectively. Correlation analysis showed positive correlation between Zn uptake and grain yield. 相似文献