A field study of Mycoplasma bovis infection in cattle     

A Feenstra  E Bisgaard Madsen  N F Friis  A Meyling  P Ahrens 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1991,38(3):195-202

After an outbreak of mastitis in cattle caused by Mycoplasma bovis a study was made in 5 herds with recent cases (principal herds) and in 4 control herds. In the principal herds, M. bovis was isolated from milk samples, nasal swabs, and from one vaginal swab. M. bovis was also isolated from nasal swabs of calves in 2 of the 4 control herds, whereas all milk samples and vaginal swabs from the control herds were negative. Evaluation of serum antibody titres to M. bovis among non-mastitic animals of 3 principal herds and 1 control herd showed no difference in distribution of the titre values, which generally were low. However, cows excreting M. bovis in the milk had high antibody titres. The way of introduction to the herds and the spread of the infection within the herds could not be established by the study, which was supplemented by a DNA restriction fragment analysis of a number of M. bovis isolates.  相似文献   

Comparison of subjective and objective test evaluations for use of Mycobacterium phlei-adsorbed serum in a dot-enzyme-linked immunosorbent assay for diagnosis of paratuberculosis in cattle.     

S Bech-Nielsen  W P Shulaw  P L Frandsen  J B Jorgensen  N C Feld  P Ahrens 《American journal of veterinary research》1992,53(8):1386-1391

Use of a dot-ELISA with serum adsorbed with Mycobacterium phlei or with nonadsorbed serum was compared. In addition, results attained using visual observation were compared with those obtained using a densitometer. Infection status of cattle was determined by results of culture of feces from a number of cattle with various degrees of exposure (low prevalence and test-negative) and disease manifestation (clinical suspect vs subclinical infection). Two paratuberculosis-negative herds, fecal culture-confirmed clinically suspect cases of paratuberculosis, and cows from 2 paratuberculosis-infected herds with diagnosis confirmed on the farm (low infection rate) were tested. Significant (P less than 0.05) increase in the dot-ELISA response was found in cattle with heavy M paratuberculosis shedding when nonadsorbed and adsorbed sera were used, compared with the response in cattle that were fecal culture-negative or were shedding M paratuberculosis at lower amounts. Paratuberculosis was diagnosed by visual determination in 29 of 44 (65.9%) of fecal culture-positive, clinically suspect cattle when nonadsorbed serum was used. Results of the visual test were negative in 85 of 93 (91.4%) of the fecal culture-negative cattle when nonadsorbed serum was used. However, when using M phlei-adsorbed serum, the sensitivity of the visual determination decreased to 34.1% (15/44), and the specificity increased to 97.8% (91/93).(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Similarity of avian paramyxovirus serotype 1 isolates of low virulence for chickens obtained from contaminated poultry vaccines and from poultry flocks     

Jørgensen PH  Handberg KJ  Ahrens P  Manvell RJ  Frost KM  Alexander DJ 《The Veterinary record》2000,146(23):665-668

At present Denmark has the status of a 'non-vaccinating' country for Newcastle disease and its poultry population should therefore be free of antibodies to avian paramyxovirus 1 (APMV-1). Three live avian vaccines against infectious bronchitis, avian encephalomyelitis, and chick anaemia which had been found to be contaminated with APMV-1 viruses of low virulence for chickens were examined. The vaccines were produced by the same company and the affected batches had been used in Denmark in 1996/97. Furthermore, APMV-1 isolates of low virulence were obtained from three commercial broiler breeder flocks, one of which had been vaccinated with two of the contaminated vaccines. The flocks belonged to the same hatchery organisation. A comparison of viral F0 gene sequences and typing of virus isolates with a panel of monoclonal antibodies showed that the vaccine and field isolates were identical.  相似文献   

Does formalin disinfection of the storeroom affect the quality of fresh eggs?     

G Ahrens 《Monatshefte für Veterin?rmedizin》1969,24(19):747-748

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Laparoscopic access to the liver and application of laparoscopic microwave ablation in 2 dogs with liver neoplasia     

Alberto Oramas  J. Brad Case  Beau B. Toskich  W. Alexander Fox‐Alvarez  Sarah E. Boston  Robson F. Giglio  Michael J. Dark 《Veterinary surgery : VS》2019,48(Z1):O91-O98

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Isolation of a nicotinamide-adenine dinucleotide (NAD)-dependent Pasteurella haemolytica-like organism from the placenta of an aborted cow.          下载免费PDF全文

A Dsilets  L Julien  K Moller  P Ahrens    R Higgins 《The Canadian veterinary journal. La revue veterinaire canadienne》1998,39(8):507

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A pilot study on the effect of oclacitinib on epicutaneous sensitization and transepidermal water loss in a colony of atopic beagle dogs          下载免费PDF全文

Rosanna Marsella  Kim Ahrens 《Veterinary dermatology》2018,29(5):439-e146

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A comparative study of epidermal tight junction proteins in a dog model of atopic dermatitis          下载免费PDF全文

Ha‐Jung Kim  Megan Cronin  Kim Ahrens  Vassi Papastavros  Domenico Santoro  Rosanna Marsella 《Veterinary dermatology》2016,27(1):40-e11

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Detection of Mycobacterium avium subsp. paratuberculosis in milk from clinically affected cows by PCR and culture     

Giese SB  Ahrens P 《Veterinary microbiology》2000,77(3-4):291-297

Milk and faeces samples from cows with clinical symptoms of paratuberculosis were examined for the presence of Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) by culture and PCR. M. paratuberculosis was cultivated in variable numbers from faeces or intestinal mucosa in eight of 11 animals. In milk from five cows (all faeces culture positive), we cultivated a few colonies of M. paratuberculosis (<100 CFU per ml). Milk samples from two cows were PCR positive (both animals were faeces culture positive, and one cow was milk culture positive). One cow was culture negative on intestinal mucosa, but culture positive in milk, and two cows were negative in culture and PCR from both faeces and milk. In conclusion, the presence of M. paratuberculosis could be detected in raw milk by PCR, but cultivation of milk was more sensitive.  相似文献   

A comprehensive phylogeny of beetles reveals the evolutionary origins of a superradiation   总被引：1，自引：0，他引：1  

Hunt T  Bergsten J  Levkanicova Z  Papadopoulou A  John OS  Wild R  Hammond PM  Ahrens D  Balke M  Caterino MS  Gómez-Zurita J  Ribera I  Barraclough TG  Bocakova M  Bocak L  Vogler AP 《Science (New York, N.Y.)》2007,318(5858):1913-1916

Beetles represent almost one-fourth of all described species, and knowledge about their relationships and evolution adds to our understanding of biodiversity. We performed a comprehensive phylogenetic analysis of Coleoptera inferred from three genes and nearly 1900 species, representing more than 80% of the world's recognized beetle families. We defined basal relationships in the Polyphaga supergroup, which contains over 300,000 species, and established five families as the earliest branching lineages. By dating the phylogeny, we found that the success of beetles is explained neither by exceptional net diversification rates nor by a predominant role of herbivory and the Cretaceous rise of angiosperms. Instead, the pre-Cretaceous origin of more than 100 present-day lineages suggests that beetle species richness is due to high survival of lineages and sustained diversification in a variety of niches.  相似文献