Effects of Serum Deprivation and Cycloheximide on Cell Cycle of Low and High Passage Porcine Fetal Fibroblasts   总被引：1，自引：0，他引：1  

MD Goissis  HVA Caetano  MG Marques  FRO de Barros  WB Feitosa  MP Milazzotto  M Binelli  MEOA Assumpção  JA Visintin 《Reproduction in domestic animals》2007,42(6):660-663

Arrest of cells in G0/G1 cell cycle phase is desired for nuclear transfer procedures. Serum starvation and cell cycle inhibitors are different ways to induce synchronization of the cell cycle. This study investigated the effects of serum starvation and cycloheximide (CHX) on the cell cycle of low (5th) and high (15th) passages fetal porcine fibroblasts. Cell cycle phases were determined using fluorescent activated cell sorting. Fifth passage fibroblast cultures had higher (p < 0.05) proportion of cells in G0/G1 only after 72 h of serum starvation (77.60 ± 0.65) when compared with non‐starved cells (71.44 ± 1.88). Serum starvation for all periods tested induced an increase (p < 0.05) on proportion of cells in G0/G1 on the 15th passage. No significant differences were observed on the 5th passage cultures exposed to CHX, although, on the 15th passage an increase on proportion of cells was observed after all periods of exposure (p < 0.05). These data indicates that high passage cells in vitro are more susceptible to serum starvation and CHX G0/G1 synchronization.  相似文献   

Embryonic and Early Foetal Losses in Cattle and Other Ruminants   总被引：1，自引：0，他引：1  

MG Diskin  DG Morris 《Reproduction in domestic animals》2008,43(S2):260-267

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Pre-parturition staphylococcal mastitis in primiparous replacement goats: persistence over lactation and sources of infection     

Iacome SC Jácome  Francisca GC Sousa  Candice MG De Leon  Denis A Spricigo  Mauro MS Saraiva  Patricia EN Givisiez  Wondwossen A Gebreyes  Rafael FC Vieira  Celso JB Oliveira 《Veterinary research》2014,45(1)

This investigation reported for the first time the occurrence of intramammary infections caused by Staphylococcus in primiparous replacement goats before parturition and the persistence of clinical Staphylococcus aureus infection during the lactation period. Subclinical infections, mainly caused by coagulase negative staphylococci (CoNS), did not persist during lactation. Genotyping analysis indicated that environment seems to play a moderate role as source of intramammary infections to goats before parturition, but causative agents of mastitis in lactating animals are not genotypically related to environmental staphylococci. The occurrence and persistence of intramammary infections in replacement goats demonstrate the need to consider those animals as potential sources of infections in dairy goat herds.  相似文献   

Termiticidal lectins from Myracrodruon urundeuva (Anacardiaceae) cause midgut damage when ingested by Nasutitermes corniger (Isoptera: Termitidae) workers          下载免费PDF全文

Thâmarah A Lima  Kenner M Fernandes  Ana Patrícia S Oliveira  Leonardo P Dornelles  Gustavo F Martins  Thiago H Napoleão  Patrícia MG Paiva 《Pest management science》2017,73(5):991-998

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Nematotoxic activity of essential oils from <Emphasis Type="Italic">Monarda</Emphasis> species     

S. Laquale  P. Avato  M. P. Argentieri  M. G. Bellardi  T. D’Addabbo 《Journal of pest science》2018,91(3):1115-1125

Bioactivity of essential oils (EOs) from Monarda species has never been investigated on phytoparasitic nematodes. In this study, the EOs from two Italian ecotypes of Monarda didyma and M. fistulosa and their main compounds, carvacrol, γ-terpinene, o-cymene, and thymol, were evaluated for their in vitro activity on the infective stages of phytoparasitic nematodes Meloidogyne incognita and Pratylenchus vulnus, as well as on M. incognita egg hatch. Soil treatments with the two EOs were also investigated for their suppressiveness on M. incognita on tomato. Both EOs were strongly active on M. incognita juveniles, as a only 1.0 μL mL^?1 LC50 value was evaluated after a 24-h exposure to both EOs, whereas a lower activity was recorded on P. vulnus (15.7 and 12.5 μL mL^?1 LC50 values for M. didyma and M. fistulosa EOs, respectively). Among the EOs’ main compounds, carvacrol was highly active also at a short exposure in low concentrations, whereas γ-terpinene and thymol were much less active on both nematode species and o-cymene showed a discrete activity on P. vulnus only at the highest concentration. Hatch percent of M. incognita eggs treated with M. didyma and M. fistulosa EOs was always significantly lower than in water or in Tween 20 and Oxamyl solutions. In the experiment in soil, the multiplication of M. incognita and gall formation on tomato roots was significantly reduced by soil treatments with both EOs. The strong nematicidal activity of both Monarda EOs may suggest them as potential sources of new sustainable nematicidal products.  相似文献