Well-defined multispecies probiotic and enzyme combination outperforms traditional fermented probiotic applications in an intensive Pacific white shrimp,Litopenaeus vannamei (Boone, 1931), culture system     

Romi Novriadi  Tri Heru Prihadi  Hartati Sri Devi Saragih  Jutta Kesselring  Benedict Standen 《Journal of the World Aquaculture Society》2023,54(1):156-166

A series of treatments were designed to evaluate the efficacy of feeding commercial multispecies probiotics feeding with enzymes and fermentation process on the growth parameters and culture environment of Pacific white shrimp, Litopenaeus vannamei (Boone, 1931), in an intensive culture system. Commercial multispecies probiotics and enzymes (PEs) were continuously applied in three different doses, namely (i) 0.2, (ii) 0.4, and (iii) 0.6 mg L⁻¹ and designated as 0.2, 0.4, and 0.6 PE during the first 30 days of intensive culture of Pacific white shrimp, L. vannamei (Boone, 1931). The probiotics were continually applied every alternate day, while the enzymes were added every sixth day throughout the trial period. The PE dose for all treated tanks was increased by 0.2 ppm after 30 days of culture and another 0.2 ppm after day 60. Meanwhile, fermentation technique, which has become the common method applied in Indonesia in the control treatment, was added on the same day with the PE group with increasing dosage, following the same trend with the 0.6 PE group. Results showed that the group receiving 0.6 PE showed higher final biomass, higher mean weight, and protein retention efficiency as well as a lower feed conversion ratio compared with the control treatment. Abundance of Vibrio spp. remained below 10³ cfu mL⁻¹ throughout the trial. Water quality indicators TAN, NO₂ N and NO₃ N peaked in weeks 3–5 and then declined after that until the end of the culture period in all tanks. This decline was significantly faster in PE-treated tanks. Multispecies PEs have potential applications in controlling Vibrio spp., maintaining proper water quality condition, and enhancing the growth of shrimp in intensive culture system.  相似文献   

Uterine horn torsion in a non-gravid cat     

De La Puerta B  McMahon LA  Moores A 《Journal of Feline Medicine and Surgery》2008,10(4):395-397

An 11-year-old female domestic shorthair cat with a previous history of having been spayed, presented with signs of abdominal distension, lethargy and anorexia of 1 week's duration. On the basis of radiological and ultrasonographic findings a tentative diagnosis of pyometra was made. Exploratory coeliotomy revealed a 900 degrees left horn uterine torsion along the longitudinal axis. Ovariohysterectomy was performed without correction of the torsion. To the authors' knowledge this is the first case report of uterine torsion in a non-gravid cat.  相似文献   

Ventral stabilization of thoracic kyphosis through bilateral intercostal thoracotomies using SOP (String of Pearls) plates contoured after a 3‐dimensional print of the spine          下载免费PDF全文

Christina B. Mathiesen DVM  Benito de la Puerta DVM  CertSAS  Diplomate ECVS  Anna M. Groth Dr med vet  Diplomate ECVDI  Scott Rutherford BVMS  CertSAS  Diplomate ECVS  Rodolfo Cappello DVM  PhD  Diplomate ECVN 《Veterinary surgery : VS》2018,47(6):843-851

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Environmental Factors Governing Soil Water Repellency Dynamics in a Pinus Pinaster Plantation in NW Spain     

Elena Benito Rueda  María Rodríguez‐Alleres  Eufemia Varela Teijeiro 《Land Degradation \u0026amp; Development》2016,27(3):719-728

Soil water repellency (SWR) is a dynamic property that changes throughout the year. The objective of this work was to identify the environmental factors governing the temporal patterns in SWR in a pine plantation in northwest Spain with a view of predicting its occurrence and persistence. For this purpose, 24 samples were collected from the soil surface (0–5 cm) at 25 different times over a 1‐year period and analysed for SWR by using water drop penetration time test and soil moisture measurements. Temporal variations in SWR exhibited a well‐defined seasonal pattern. The soil surface was largely wettable from late autumn to early spring and extremely water repellent during summer and early autumn. Repellency persistence was rather variable during spring. There was highly significant correlation between SWR and soil moisture content. The moisture range defining the presence or absence of repellency under field conditions was 22–57%. There were also significant correlations with the target variables (maximum temperature, minimum temperature, precipitation and water balance during variably long antecedent periods), with coefficients that increased with increasing length of the antecedent period considered. The moisture content of soil at the time of sampling and the average maximum temperature for the 28 days before sampling are the best predictors of occurrence of SWR and its persistence in different seasons. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

Histopathological and immunohistochemical findings in lymphoid tissues of the endangered Iberian lynx (Lynx pardinus)     

Peña L  Garcia P  Jiménez M  Benito A  Pérez Alenza M  Sánchez B 《Comparative immunology, microbiology and infectious diseases》2006,29(2-3):114-126

The Iberian lynx (Lynx pardinus) is the most threatened wild feline in the world. Little is known about the diseases and pathology that affect this animal. The aim of this study was to evaluate the histopathological status of the peripheral lymphoid tissues and thymus of Iberian lynxes necropsied between 1998 and 2003. Seventeen animals including females (n=8) and males (n=9), age range of 10 months to 16 years, with different causes of death were histopathologically and immunohistochemically (anti-CD3, CD79, MAC387, CD68) studied. Feline immunosuppressive virus laboratorial tests were negative. Five individuals presented neoplasia and/or tuberculosis. All animals presented some degree of both B and T cells depletion in peripheral lymphoid tissues and follicular hyalinosis in the center of depleted follicles. A viral origin of the lymphoid depletion is postulated although other causes (inbreeding, stress, toxic) are not ruled out. The loss of the effectiveness of the immune system increases the vulnerability of the critically endangered Iberian lynx to pathogens.  相似文献   

Fungal and plant gene expression during synchronized infection of tomato leaves by Botrytis cinerea     

Ernesto P. Benito  Arjen ten Have  John W. van 't Klooster  Jan A.L. van Kan 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(2):207-220

An inoculation procedure was developed to obtain efficient and synchronous infection on detached tomato leaves by Botrytis cinerea. In spray-inoculated leaves incubated at 20 °C, the infection process consisted of three phases: the formation of primary necrotic lesions (until 20 hpi), a quiescent phase (20-72 hpi), and the expansion of a proportion of the primary lesions (from 72 hpi onwards), resulting in full tissue maceration. At 4 °C, the infection progressed slowly but steadily without inducing necrotic responses in the host. The actin and -tubulin genes of B. cinerea were cloned, characterized and used as probes on blots containing RNAs from leaves at various stages of the infection. The genes displayed a similar expression pattern throughout the infection and the hybridization signal reflected the amount of fungal biomass. The actin mRNA accumulated to higher levels than the -tubulin mRNA. Tomato PR protein mRNAs (chitinase, -1,3-glucanase and PR-1) were induced during the infection, albeit with different kinetics and to different levels. At 20 °C, -1,3-glucanase and PR-1 mRNAs were induced more rapidly than chitinase mRNAs. At 4 °C, mRNAs encoding extracellular -1,3-glucanase and intracellular, as well as extracellular chitinase were hardly induced.  相似文献   

Ecology of soil springtails (Collembola, Insecta) from pine woods and Rhododendron shrublands in the Central and Eastern Pyrenees (North Spain)     

Jos Carlos Simn Benito  Maria Jos Luciez Snchez 《Pedobiologia》2000,44(3-4)

The collembological composition of samples from the Central and Western Pyrenees (Northern Spain) was studied. Soils from two biotopes (pine forest and Rhododendron shrub) were studied in Nuria and Vallibierna, using different diversity indices and multivariate analyses. Ten species were found that were endemic to the Iberian Peninsula. Three of these are exclusive to the Pyrenees: Ceratophysella elegans, Hypogastrura dasiensis and Protachorutes pyrenaeus. The greatest diversity was observed in the soils of Rhododendron, above all at Nuria. Collembola were most poorly represented in the pine forest of Vallibierna, where the endemic Hypogastrura meridionalis was the dominant species. Multivariate analyses indicated that the latter species characterizes this pine forest and separates it from all other biotopes studied. The evenness (E) was most pronounced in Nuria, where Folsomia manolachei was the characteristic species.  相似文献   

逐步判别分析在呼市地区长爪沙鼠年龄鉴定中的应用     

薛河儒 《内蒙古农业大学学报(自然科学版)》1992,(3)

长爪沙鼠是我区的主要鼠类之一,为探讨该鼠种在农林牧业系统中的作用,并有效地控制其危害,提高防治鼠害的技术水平和效果,研究该种群的年龄组成及鉴定所属年龄组是非常必要的。本文利用逐步判别分析法对3个年龄组中的72个个体作了分析,结果表明判别准确率达90.28％。在13个特征中选出了来自头骨外形的5个最显著的特征。在今后的实际工作中,为了鉴定所属年龄组,只需简单地测量这5个指标而不需要测量体重、体长以及观察毛色等特征。  相似文献   

Staphylococcus aureus Panton-Valentine leukocidin causes necrotizing pneumonia     

Labandeira-Rey M  Couzon F  Boisset S  Brown EL  Bes M  Benito Y  Barbu EM  Vazquez V  Höök M  Etienne J  Vandenesch F  Bowden MG 《Science (New York, N.Y.)》2007,315(5815):1130-1133

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Ultrafiltration of a Waste Emulsified Cutting Oil using Organic Membranes   总被引：1，自引：0，他引：1  

Benito  J. M.  Ebel  S.  Gutiérrez  B.  Pazos  C.  Coca  J. 《Water, air, and soil pollution》2001,128(1-2):181-195

The influence of operating conditions on permeate flux was investigated using organic ultrafiltration membranes to treat a synthetic cutting-oil emulsion containing 0.05 to 0.8 wt. % oil. A commercial cutting oil, Alba-Kool, and four organic ultrafiltration membranes, two made of polysulfone (PM10 and PM30) and the other two of regenerated cellulose (YM10 andYM30), with cut-off sizes of 10 000 or 30 000 Dalton, were used.All experiments were performed in a commercially available stirred ultrafiltration cell. Within the range of operating conditions studied, it was found that the highest fluxes wereobtained at the following conditions: crossflow velocity (rotation speed) of 375 rpm, temperature of 40 °C andtransmembrane pressure of 0.1–0.15 MPa. Oil rejections between99 and 99.9% were obtained. Complete membrane regeneration wasachieved with a 2 vol. % aqueous solution of a commercial cleaning agent (Derquim+, Panreac).  相似文献