Chromosomal location of genes controlling 6-phosphogluconate dehydrogenase,glucose-6-phosphate dehydrogenase and glutamate dehydrogenase isozymes in cultivated rye     

J. Salinas  C. Benito 《Euphytica》1983,32(3):783-790

Summary The 6-phosphogluconate dehydrogenase (6-PGD), glucose-6-phosphate dehydrogenase (G-6-PD) and glutamate dehydrogenase (GDH) zymogram phenotypes of wheat, rye and their aneuploid derivates were determined. At least three genes involved in the production of 6-PGD isozymes were located on chromosome arms 4RL, 6RL and 2RL of Imperial rye, King II rye and Dakold rye. Evidence was obtained that at least one gene located on chromosome arm 5RS controls G-6-PD isozyme activities in these varieties of rye and one gene involved in the production of GDH isozymes was located on chromosome arm 2RS of Imperial, King II and Dakold rye. The results indicate that the 6-PGD isozymes exist as dimers and monomers. No possible structure of the G-6-PD and GDH could be determined.  相似文献   

印度南瓜单瓜产籽数与影响因素分析   总被引：4，自引：0，他引：4  

崔世茂  赵清岩 《华北农学报》1996,11(1):114-117

通过对印度南瓜果实主要数量性状的通径分析表明，各性状与单瓜产籽数的相关程度依次为单瓜重（０．８７６５）〉瓜横径（０．８６２２）〉瓜纵径（－０．７４７２）〉籽粒长（０．５６９４）〉籽粒宽（０．４２８４）〉千粒重（－０．０６０４）。各性状对单瓜产籽数起直接作用大小依次为瓜横径（０．４７４９）〉单瓜重（０．３６３８）〉籽粒宽（０．２１２２）〉瓜纵径（－０．１７４１）〉籽粒长（－０．１２５７）〉千粒重（－  相似文献   

Hematologic and biochemical changes during canine agility competitions     

Rovira S  Muñoz A  Benito M 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2007,36(1):30-35

BACKGROUND: Normal response to different types of exercise needs to be established before performance, training level, or exercise intolerance can be assessed. OBJECTIVES: The aim of this research was to describe the hematologic and biochemical changes that dogs undergo during an agility competition. METHODS: Fifteen dogs were subjected to an agility test that covered a distance of 360-400 m with 40 obstacles. Basal venous blood samples were drawn 1 week before the competition, immediately after exercise, and at 5, 15, and 30 minutes of recuperation. A CBC, plasma biochemical profile, and lactate concentration were determined by standard methods using automated analyzers. Serum cortisol concentration was measured by competitive immune-enzyme analysis; insulin was measured by sandwich ELISA. RESULTS: Hematologic response to the agility test consisted of significant increases in RBC count, hemoglobin concentration, and HCT, with no change in total protein concentration. Serum triglyceride concentrations were increased postexercise, with highest values observed at 30 minutes of recuperation. Postexercise lactate concentrations exceeded the anaerobic threshold of 4 mmol/L. Serum lactate dehydrogenase activity was significantly higher after 30 minutes of recuperation. Significant differences were not observed in other analytes. CONCLUSIONS: Our results indicate that agility competitions induce mild to moderate changes in hematologic and biochemical results consistent with splenic contraction, increased lipolysis, and utilization of anaerobic pathways involved in energy resynthesis in muscle.  相似文献   

Bronchioloalveolar carcinoma not related to jaagsiekte sheep retrovirus in a goat     

Ortín A  Benito AA  Lacasta D  Ferrer LM  De Las Heras M 《Veterinary pathology》2007,44(5):710-712

A spontaneous lung tumor in a 5-year-old goat of the Murciano-Granadina breed is described in this paper. Clinical signs of cachexia and tachypnoea were evident, and a considerable amount of white mucous foamy fluid was discharged from the nostrils when the animal's head was lowered. A lung tumor with the characteristics of bronchioloalveolar carcinoma was detected during histopathologic examination. The tumor cells were positive for surfactant proteins C and B, confirming that alveolar type II cells were the origin of the neoplasia. Tumor samples were tested by polymerase chain reaction, immunoblotting, and immunohistochemistry for the presence of Jaagsiekte sheep retrovirus (JSRV) and enzootic nasal tumor virus (ENTV), another retrovirus very closely related to JSRV, but all tests were negative. Therefore, this is the first reported case of spontaneous bronchioloalveolar carcinoma not related to JSRV or ENTV infection in a goat.  相似文献   

Stabilized municipal sewage sludge addition to improve properties of an acid mine soil for plant growth   总被引：1，自引：0，他引：1  

M. D. Mingorance  S. Rossini Oliva  Benito Valdés  F. J. Pina Gata  Eduardo O. Leidi  I. Guzmán  Aránzazu Peña 《Journal of Soils and Sediments》2014,14(4):703-712

Purpose

Degraded soils, such as those encountered in areas of mine activities, need to be ameliorated by liming to correct soil acidity and by addition of organic inputs to improve soil properties and fertility.

Materials and methods

Non-amended mine soil and soil amended with stabilized sewage sludge were incubated for 45 days. Soil physicochemical and biological indicators were periodically measured along incubation and other enzyme activities at the end of incubation. In improved soils, a study of plant development in 250-g pots was carried out with three vegetal species: tomato, rye grass and ahipa. Germination and mortality rates, biomass production and photosynthetic pigments were measured.

Results and discussion

Soil incubation with sewage sludge slightly increased soil pH and led to an enhancement of soil electrical conductivity, organic carbon and dehydrogenase activity, especially for the higher doses (5 and 10%). However soil respiration was more promoted with the 2% dose, pointing to a possible toxic effect of the sludge. At the end of incubation, physicochemical and biological properties were in general enhanced. Biomass production was improved in tomato and rye grass by sewage sludge addition (more at the 2% dose), whilst ahipa growth was not affected by sewage sludge treatments. Tomato mortality reached 73% with high sludge doses (10%).

Conclusions

According to this set of parameters, amendment with sewage sludge of a limed acid mine soil would be considered as a good strategy for soil amelioration in view of plant establishment and development.  相似文献   

Is nitrogen fertilization in the nursery a suitable tool for enhancing the performance of Mediterranean oak plantations?     

Pedro Villar-Salvador  Juan L. Peñuelas  Juan L. Nicolás-Peragón  Luis F. Benito  Susana Domínguez-Lerena 《New Forests》2013,44(5):733-751

Mediterranean oaks frequently have poor performance in forest restoration projects. We analyzed if nitrogen (N) fertilization during nursery cultivation enhances the outplanting performance of two ecologically distinct oaks, Quercus coccifera L. an evergreen shrub, and Quercus faginea L., a deciduous tree. Additionally, we compared the sufficiency N levels of both oaks and if root growth capacity (RGC) and photosynthesis testing at low air temperature better discriminated among fertilization treatments than testing at mild temperature. Plants were cultivated under five N fertilization rates (0, 30, 75, 150 and 200 mg N plant^?1) for one growing season. At the end of the cultivation period several plant material and performance attributes were measured. RGC and photosynthesis were assessed at mild temperature and cool conditions in Q. coccifera, while in Q. faginea these attributes were only analyzed at mild temperature conditions. Q. coccifera seedlings were transplanted into four terraces that imposed increasing drought stress, while Q. faginea seedlings were transplanted into an abandoned wheat cropland. Sufficiency level was higher for the tree (150 mg N plant^?1) than for the shrub (75 mg N plant^?1) Nitrogen-deprived Q. coccifera plants had higher frost damage than remaining treatments, which did not differ among them. Fertilization in Q. faginea did not affect photosynthesis rate but it delayed leaf senescence and abscission. RGC increased with fertilization in both species. Testing temperature did not change the effect of fertilization on RGC in Q. coccifera. Photosynthesis in cool conditions discriminated N-deprived seedlings from the remaining treatments, but treatments did not differ in photosynthesis at mild temperature. Fertilization enhanced outplanting survival in Q. coccifera, primarily in the terraces imposing stronger drought stress, while fertilization only increased growth in the terraces imposing mild drought stress. Overall, survival and growth was positively related to seedling size at planting in both oaks. Additionally, Q. coccifera survival was also positively related to RGC in the harsh planting conditions. Field growth was positively related to RGC and plant N concentration in both oaks but the relation was weak or nonexistent under harsh conditions in Q. coccifera. We conclude that fertilization in the nursery is an important tool for improving the success of oak plantations in Mediterranean continental sites, and that large and N-rich seedling have greater field performance than small and low tissue N concentration seedlings, especially in harsh field sites.  相似文献   

改进C-V模型在YCbCr空间下的羊体图像分割     

白明月  薛河儒  姜新华  周艳青 《中国农业大学学报》2018,23(10):137-143

针对羊体图像背景复杂、分割难以及不同光照条件干扰羊体图像的问题,采用一种基于YCbCr空间改进C-V主动轮廓模型的分割方法,对具有复杂背景的羊体图像分割进行研究。结果表明:1)根据羊体图像的颜色特点,对羊体图像进行从RGB空间到YCbCr空间的转换能克服拍摄环境中光照对羊体的影响;2)利用手动勾画羊体的粗略轮廓构造预处理水平集,对其内部、外部以及边界进行划分后可以演化羊体图像的轮廓。试验证明改进C-V模型能对复杂背景下的羊体图像进行准确分割,分割结果能够应用到后续羊体测量点的识别中。  相似文献   

aquasaúde: A mobile application for educational training on prevention of shrimp pathogen‐associated diseases          下载免费PDF全文

Eduardo Henrique P Pooch  Arturo Sanchez‐Paz  Maria Helena Fermino  Kelly Cristina T de Brito  Benito de Brito  Luis Alberto Romano  Paulo César Abreu  Marcia Regina Stech  Silvio César Cazella  Lissandra S Cavalli 《Aquaculture Research》2018,49(7):2597-2602

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Ichthyosis fetalis in a cross‐bred lamb          下载免费PDF全文

Antonio Carlos Lopes Câmara  Pedro Augusto Cordeiro Borges  Silvano Alves Paiva  Felipe Pierezan  Benito Soto‐Blanco 《Veterinary dermatology》2017,28(5):516-e125

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Bioassay-guided isolation of an anti-ulcer chromene from Eupatorium aschenbornianum: Role of nitric oxide,prostaglandins and sulfydryls     

María Elena Sánchez-Mendoza  Benito Reyes-Trejo  Paula Sánchez-Gómez  Juan Rodríguez-Silverio  Carlos Castillo-Henkel  Humberto Cervantes-Cuevas  Jesús Arrieta 《Fitoterapia》2010

Eupatorium aschenbornianum is considered useful in the treatment of gastric ulcer. In the current study the validity of this practice was tested by using the experimental model of an ethanol induced gastric ulcer in rats. The results show that E. aschenbornianum had gastroprotective activity, that the hexane extract had the highest protective activity (85.65 ± 4.76%), and that encecanescin isolated from this extract was the main active gastroprotective agent. The effect elicited by encecanescin was attenuated by N^G-nitro-l-arginine methyl ester, N-ethylmaleimide and indomethacin, which suggests that NO, prostaglandins and sulfydryl groups are involved in the mechanisms of gastroprotective action.  相似文献