Response of high mountain landscape to topographic variables: Central pyrenees     

Gabriel del Barrio  Bernardo Alvera  Juan Puigdefabregas  Carlos Diez 《Landscape Ecology》1997,12(2):95-115

An objective method for inductively modelling the distribution of mountain land units using GIS managed topographic variables is presented. The landscape of a small high mountain catchment in the Spanish Pyrenees, covered with grassland, was classified in ten land units by hierarchical agglomerative clustering, using a sample of 194 random plots, in which classes of vegetation, soils and landforms were defined. Additionally, seven layers of topographic variables (altitude, slope angle, aspect, solar radiation, topographic wetness index, specific catchment area, and regolith thickness) were created from a Digital Elevation Model. The affinity of each land unit to the topographic variables was calculated using Binary Discriminant Analysis (BDA), after dichotomising the latter around their mean values. Then, the distribution of each land unit was predicted by boolean operations combining step by step distributions for the seven topographic variables ordered, for each unit, after the absolute values of the Haberman’s residuals in BDA. The predicted distributions were tested (χ²) against that of the observed sampling plots. From the original ten land units, the distributions of eight of them were successfully predicted (four are related to the slope sequence, two reflect the water accumulation in the soil, and two respond to geomorphic processes) while the remaining two had to be rejected. Part of the catchment (39%) was not assigned to any land unit, probably because more distributed variables accounting for snow distribution are necessary.  相似文献   

Growth and nutritional disorders of coffee cultivated in nutrient solutions with suppressed macronutrients     

Rilner Alves Flores  Bernardo Melo Montes Nogueira Borges  Hilário Júnior Almeida  Renato De Mello Prado 《Journal of plant nutrition》2016,39(11):1578-1588

The objective was to evaluate the effect of omitting macronutrients in the nutrients solution on growth characteristics and nutritional status of coffee. The treatments were complete nutrients solutions and solutions with nutrient omission: N (nitrogen), P (phosphorus), K (potassium), Ca (calcium), Mg (magnesium) and S (sulfur). The experiment was carried out under greenhouse conditions with 3 replicates in a completely random design. Plant height, number of leaves per plant, stem diameter, relative chlorophyll index, photosynthesis rate, stomatal conductance, transpiration, carbon dioxide (CO₂) concentration, dry matter, content levels of macronutrients in plant aerial part and root system, and nutritional disorders were evaluated. Macronutrients suppression affected nutrients concentration in many plant parts, inducing the appearance of symptoms characteristic of each nutrient. The most limiting nutrients for coffee plants development were nitrogen and calcium, reflected in the lower dry matter accumulation and nitrogen the most required.  相似文献   

Macronutrient omission and the development and nutritional status of basil in nutritive solution     

Bernardo Melo Montes Nogueira Borges  Rilner Alves Flores  Hilário Júnior de Almeida  Leandro Rossato Moda  Renato de Mello Prado 《Journal of plant nutrition》2016,39(11):1627-1633

The correct nutrition of basil (Ocinum basilicum L.) is important to increase its production and quality; however, few papers have deal with this subject. The aim of this work was to evaluate the effects of omission of individual macronutrients on the growth and nutritional status of basil cultivated in nutritive solution. The treatments consisted of nutrient solutions with nitrogen, phosphorus, potassium, calcium, magnesium or sulfur (N, P, K, Ca, Mg, or S) omissions and a complete solution treatment. The plants were cultivated in 8 L plant pots. Plant height, number of leaves per plant, leaf area, relative chlorophyll index, net photosynthesis rate, stomatal conductance, plant dry matter, concentration levels of macronutrients in the aerial part and root system, and nutritional disorders were all evaluated. Nutrient omission was a limiting factor for plant development, substantially reducing its growth. There was also a considerable decrease in nutrient accumulation when compared to the control treatment.  相似文献   

Using detection dogs and genetic analyses of scat to expand knowledge and assist felid conservation in Misiones,Argentina     

Karen E. DeMATTEO  Miguel A. RINAS  Carina F. ARGÜELLES  Bernardo E. HOLMAN  Mario S. DI BITETTI  Barbara DAVENPORT  Patricia G. PARKER  Lori S. EGGERT 《Integrative zoology》2014,9(5):623-639

Many carnivores require large ranges to meet their ecological and energetic needs; however, anthropogenic changes threaten species and their habitats. Camera traps have been used to effectively collect data on carnivores in a variety of habitat types; however, a single survey effort is typically limited to species that have similar body size, habitat use and movement patterns, and individual identification of animals is not always possible. We evaluated whether scat detection dogs could effectively survey for 4 wide‐ranging felids that vary in these characteristics: jaguars (Panthera onca), pumas (Puma concolor), ocelots (Leopardus pardalis) and oncillas (Leopardus tigrinus). From June to October 2009 and May to August 2011, a detection dog‐handler team detected 588 scats, from which 176 unique genotypes were detected. We assigned sex to 84.7% of the genotyped scats and identified 55 individuals multiple times. The effectiveness of these noninvasive techniques (detection dogs and genetic analyses of scat) not only opens the door for additional studies in areas that were previously difficult or impossible with standard survey techniques, but also provides conservationists with a set of tools that overcome some of the limitations associated with the use of camera traps alone.  相似文献   

Effects of simple and double grafting melon plants on mineral absorption,photosynthesis, biomass and yield     

Alberto San Bautista  Angeles Calatayud  Sergio G. Nebauer  Bernardo Pascual  José Vicente Maroto  Salvador López-Galarza 《Scientia Horticulturae》2011

The Spanish type cultivar ‘Piel de Sapo’ (Cucumis melo L. var. saccharinus), has a limited compatibility with the Cucurbita maxima × Cucurbita moschata hybrids currently used as rootstocks. Double grafting can be used to improve compatibility between rootstock and scion by means of an intermediate rootstock compatible with both. Non-grafted, single, and double grafted melon plants of the cultivar Piel de Sapo were evaluated for water, nutrient absorption, photosynthesis activity, biomass production in early phases, as well as for yields and fruit quality in a long term trial. The hybrid ‘Shintoza’ (C. maxima × C. moschata) was used as rootstock, and the cantaloupe type melon cultivar ‘Sienne’ as an intermediate scion. Grafting did not affect net photosynthetic values, yet increased water use efficiency (35%). Double grafted plants increased aerial dry weights (66% and 31% with respect to non-grafted and simple graft plants, respectively), and also increased capacity for uptaking beneficial minerals (between 61% and 13% and particularly for NO₃⁻, P, K, Ca, Mn, and Zn) with respect to non-grafted and single grafted plants. The quantum efficiency PSII photochemistry values increased in double grafted plants (12%) with respect to the control plants. Consequently, double grafting on a vigorous rootstock such as ‘Shintoza’, with an intermediate scion, results in improved mineral and water absorption and achieves an increase in ion influx to the scion – so enabling an increase in light photosynthetic reaction and biomass. Double grafted plants increased fruit yield when compared to simple grafted and non-grafted plants (12% and 56%, respectively) and did not affect fruit quality in terms of °Brix and colour. In conclusion, double grafting presents several beneficial aspects that are counter-balanced by the extra cost of the technique. The difference in yields reflects compatibility problems.  相似文献   

The effects of ivermectin on transmission of Onchocerca volvulus   总被引：2，自引：0，他引：2  

E W Cupp  M J Bernardo  A E Kiszewski  R C Collins  H R Taylor  M A Aziz  B M Greene 《Science (New York, N.Y.)》1986,231(4739):740-742

Ivermectin, given to onchocerciasis patients as a single oral dose of 200 micrograms per kilogram of body weight, substantially reduced the uptake of Onchocerca volvulus microfilariae by Simulium yahense, an efficient black fly vector of the parasite in the tropical rain forests of West Africa. Three months after treatment, patients given ivermectin infected flies at a significantly lower rate than those who had received diethylcarbamazine or placebo, thereby reducing the number of developing larvae in the vector population. This diminished rate of infectiousness was also evident 6 months after treatment. These results strongly suggest that ivermectin could be effective in interrupting transmission of Onchocerca volvulus for epidemiologically important periods of time.  相似文献   

Dissecting apicoplast targeting in the malaria parasite Plasmodium falciparum     

Foth BJ  Ralph SA  Tonkin CJ  Struck NS  Fraunholz M  Roos DS  Cowman AF  McFadden GI 《Science (New York, N.Y.)》2003,299(5607):705-708

Transit peptides mediate protein targeting into plastids and are only poorly understood. We extracted amino acid features from transit peptides that target proteins to the relict plastid (apicoplast) of malaria parasites. Based on these amino acid characteristics, we identified 466 putative apicoplast proteins in the Plasmodium falciparum genome. Altering the specific charge characteristics in a model transit peptide by site-directed mutagenesis severely disrupted organellar targeting in vivo. Similarly, putative Hsp70 (DnaK) binding sites present in the transit peptide proved to be important for correct targeting.  相似文献   

Nitrate/ammonium ratio effects on nutrient solution pH,dry matter yield,and nitrogen uptake of sorghum 1     

L. M. Bernardo  R. B. Clark  J. W. Maranville 《Journal of plant nutrition》2013,36(10):1389-1400

Abstract

Sorghum [Sorghum bicolor (L.) Moench] seedlings were grown in nutrient solutions in a growth chamber to investigate the effects of different ratios of NO₃ ^– and NH₄ ⁺ on nutrient solution pH, dry matter yield, and N uptake. Nutrient solutions and plant tissues were assayed throughout the time plants grew in the nutrient solutions.

Nutrient solution pH depended on source of N. The pH rose to near 8 with NO₃ ^– as the sole source of N and decreased to near or below 4 with NH₄ ⁺ added to the solutions. Upon depletion of NH₄ ⁺ from solution, pH values rose abruptly to near 8 and remained near this value throughout the duration of the experiments. Dry matter yield was generally higher for plants grown with some NH₄ ⁺ compared to plants grown with NO₃ ^– alone. Nitrogen uptake was generally higher in plants grown with the higher proportions of NH₄ ⁺. Nitrogen concentrations remained unchanged with plant age as NO₃ ^–/ NH₄ ⁺ ratio varied. For solutions low in NH₄ ⁺, N concentrations in roots increased with plant age. Severe Fe deficiency appeared in plants when solution pH reached and remained above 7.  相似文献   

Subfield crop yields and temporal stability in thousands of US Midwest fields     

Maestrini  Bernardo  Basso  Bruno 《Precision Agriculture》2021,22(6):1749-1767

Understanding subfield crop yields and temporal stability is critical to better manage crops. Several algorithms have proposed to study within-field temporal variability but they were mostly limited to few fields. In this study, a large dataset composed of 5520 yield maps from 768 fields provided by farmers was used to investigate the influence of subfield yield distribution skewness on temporal variability. The data are used to test two intuitive algorithms for mapping stability: one based on standard deviation and the second based on pixel ranking and percentiles. The analysis of yield monitor data indicates that yield distribution is asymmetric, and it tends to be negatively skewed (p?<?0.05) for all of the four crops analyzed, meaning that low yielding areas are lower in frequency but cover a larger range of low values. The mean yield difference between the pixels classified as high-and-stable and the pixels classified as low-and-stable was 1.04 Mg ha^?1 for maize, 0.39 Mg ha^?1 for cotton, 0.34 Mg ha^?1 for soybean, and 0.59 Mg ha^?1 for wheat. The yield of the unstable zones was similar to the pixels classified as low-and-stable by the standard deviation algorithm, whereas the two-way outlier algorithm did not exhibit this bias. Furthermore, the increase in the number years of yield maps available induced a modest but significant increase in the certainty of stability classifications, and the proportion of unstable pixels increased with the precipitation heterogeneity between the years comprising the yield maps.

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The critical importance of an undammed river segment to the reproductive cycle of a migratory Neotropical fish     

Joo de Magalhes Lopes  Paulo Santos Pompeu  Carlos Bernardo Mascarenhas Alves  Alexandre Peressin  Ivo Gavio Prado  Fbio Mineo Suzuki  Susanne Facchin  Evanguedes Kalapothakis 《Ecology of Freshwater Fish》2019,28(2):302-316

Biotelemetry, ichthyoplankton and genetic data can provide detailed information about the migratory dynamics and reproductive cycle of freshwater fishes. However, few studies have combined these techniques in Neotropical systems. The objective of this study was to examine the migratory and reproductive dynamics of Prochilodus costatus in the São Francisco River watershed, south‐east Brazil, by comparing the ecological importance of two rivers to the species, an undammed segment of the São Francisco River and a dammed segment of one of its main tributaries, the Pará River. In total, 215 fish were radio‐tagged over three years (2014–16). Eggs and larvae were sampled at seven locations and analysed by PCR to identify Prochilodus spp. ichthyoplankton. Most radio‐tagged individuals (97%) used the undammed segment of the São Francisco River as spawning migration route, even those captured and released in the Pará River. Fish migrated to spawn from late September to late November with the arrival of the rains and returned to feeding sites from December to May after spawning. The highest densities of fish eggs and larvae were recorded in the upper reaches of the São Francisco River during months of peak river discharge. Returning fish showed high fidelity to sites occupied before spawning migration. Fish spent roughly 71% of the year at feeding sites, 25% at spawning sites and 4% moving between them. This study provides novel information about the migratory dynamics of Neotropical fishes and underscores the key role of undammed river segments for the conservation of Neotropical migratory fish species.  相似文献