Biomechanical Comparison of Two Plating Techniques for Fixation of Acetabular Osteotomies in Dogs     

R. J. Hardie  DVM  Diplomate ACVS    J. E. A. Bertram  PhD    R. J. Todhunter  BVSc  MS  PhD Diplomate ACVS    E. J. Trotter  DVM  MS  Diplomate ACVS 《Veterinary surgery : VS》1999,28(3):148-153

OBJECTIVE: To compare the failure properties of a 5-hole, 2.7-mm curved acetabular plate (AP) to a 5-hole, 3.5-mm reconstruction plate (RP) when applied to acetabular osteotomies. STUDY DESIGN: Cadaver study. ANIMALS OR SAMPLE POPULATION: Pelves of 8 mature, large-breed dogs. METHODS: A 5-hole, 2.7-mm AP and a 5-hole, 3.5-mm RP were contoured and applied to the dorsal acetabulum of each pelvis. A central acetabular fracture was simulated after plate application by a transverse osteotomy with a fine saw. Each acetabulum was loaded in a weight-bearing direction. A load-deformation curve was produced for each construct, and biomechanical properties of the AP and RP were compared with the Student's paired t-test. A P value of < .05 was considered significant. RESULTS: For the AP and RP composite respectively, the mean +/- SD maximum load to failure was 2,721 +/- 632 N and 2,488 +/- 800 N, the stiffness was 4.8 +/- 1.8 N/m and 5.3 +/- 1.9 N/m, and the energy absorbed was 15.1 +/- 5.2 Nm and 16.3 +/- 8.3 Nm. None of these differences was statistically significant. CONCLUSIONS: Both fixation techniques provided comparable strength, stiffness, and energy absorbed under the loading conditions of this study. CLINICAL RELEVANCE: Because of the relative ease of application, the 2.7-mm curved AP may be the practical choice for acetabular fracture repair in large dogs.  相似文献   

Phase-matched generation of coherent soft X-rays     

A Rundquist  CG Durfee  Z Chang  C Herne  S Backus  MM Murnane  HC Kapteyn 《Science (New York, N.Y.)》1998,280(5368):1412-1415

Phase-matched harmonic conversion of visible laser light into soft x-rays was demonstrated. The recently developed technique of guided-wave frequency conversion was used to upshift light from 800 nanometers to the range from 17 to 32 nanometers. This process increased the coherent x-ray output by factors of 10(2) to 10(3) compared to the non-phase-matched case. This source uses a small-scale (sub-millijoule) high repetition-rate laser and will enable a wide variety of new experimental investigations in linear and nonlinear x-ray science.  相似文献   

Rattenbekämpfung auf chemischer Grundlage     

L. Bertram 《Journal of pest science》1951,24(3):42-44

Ohne Zusammenfassung  相似文献   

Quality of sour cherry juice of different clones and cultivars (Prunus cerasus L.) determined by a combined sensory and NMR spectroscopic approach     

Clausen MR  Pedersen BH  Bertram HC  Kidmose U 《Journal of agricultural and food chemistry》2011,59(22):12124-12130

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NMR-based metabolomics reveals that conjugated double bond content and lipid storage efficiency in HepG2 cells are affected by fatty acid cis/trans configuration and chain length     

Najbjerg H  Young JF  Bertram HC 《Journal of agricultural and food chemistry》2011,59(16):8994-9000

In the present study the metabolic response to various fatty acids was investigated in HepG2 cells by using a (1)H NMR-based approach. To elucidate the effect of cis/trans configuration, the cells were exposed to either oleic acid (C18:1 cis-9), elaidic acid (C18:1 trans-9), vaccenic acid (C18:1 trans-11), linoleic acid (C18:2), or palmitic acid (C16:0), and multivariate data analysis revealed a strong effect of fatty acid on the lipophilic metabolite fraction. Inspection of the spectra revealed that the difference between the observed responses could be ascribed to the appearance of resonances from conjugated double bonds (5.65, 5.94, and 6.28 ppm) in cells exposed to vaccenic acid, revealing that vaccenic acid upon uptake by the HepG2 cells is converted into a conjugated fatty acid. Upon exposure of the HepG2 cells to either butyric acid (C4:0), caproic acid (C6:0), lauric acid (C12:0), myristic acid (C14:0), or palmitic acid (C16:0), an effect of fatty acid length was also evident, and data indicated that short-chain fatty acids (C4-C6) are immediately converted, whereas medium-long-chain fatty acids (C12-16) are incorporated into triglycerides and deposited in the cells. In conclusion, the present study demonstrates that (1)H NMR spectroscopy is a useful method for studying the uptake of fatty acids in in vitro cells.  相似文献   

Evidence for genetic linkage of Alzheimer's disease to chromosome 10q     

Bertram L  Blacker D  Mullin K  Keeney D  Jones J  Basu S  Yhu S  McInnis MG  Go RC  Vekrellis K  Selkoe DJ  Saunders AJ  Tanzi RE 《Science (New York, N.Y.)》2000,290(5500):2302-2303

Recent studies suggest that insulin-degrading enzyme (IDE) in neurons and microglia degrades Abeta, the principal component of beta-amyloid and one of the neuropathological hallmarks of Alzheimer's disease (AD). We performed parametric and nonparametric linkage analyses of seven genetic markers on chromosome 10q, six of which map near the IDE gene, in 435 multiplex AD families. These analyses revealed significant evidence of linkage for adjacent markers (D10S1671, D10S583, D10S1710, and D10S566), which was most pronounced in late-onset families. Furthermore, we found evidence for allele-specific association between the putative disease locus and marker D10S583, which has recently been located within 195 kilobases of the IDE gene.  相似文献   

Retraction     

Bao Z  Batlogg B  Berg S  Dodabalapur A  Haddon RC  Hwang H  Kloc C  Meng H  Schön JH 《Science (New York, N.Y.)》2002,298(5595):961

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Effects of Prolonged in vitro Culture and Cryopreservation on Viability,DNA Fragmentation,Chromosome Stability and Ultrastructure of Bovine Cells from Amniotic Fluid and Umbilical Cord     

ER da Cunha  CF Martins  CG Silva  HC Bessler  SN Báo 《Reproduction in domestic animals》2014,49(5):806-812

The objective of this work was to study cellular types that did not participated in the gastrulation process, amniotic fluid cells (AFCs) and umbilical cord cells (UCCs), in conditions of long‐term culture and cryopreserved with different solutions. The AFCs and UCCs were used in a comparative study with ear fibroblast cells (EFCs) that were cultured in vitro until 20 cellular passages and cryopreserved in 10% dimethylsulphoxide (DMSO), 5% dimethyl formamide (DMF) and 7% glycerol (Gly) solutions. The cellular viability, ultrastructure, DNA fragmentation and chromosome stability were evaluated to determine the cellular type most resistant. In all cell types, it was possible to evaluate the AFCs until 15 passages and UCCs until 20 passages with different periods of cellular growth to reach the confluence phase. Solutions containing 10% DMSO ensured viability of 90.33 ± 5.58%, 90.56 ± 4.40% and 81.90 ± 3.31%, respectively for EFCs, AFCs and UCCs, being significantly more efficient and with less variation than other cryoprotectant solutions. The AFCs were more sensitive to cryopreservation and presented low viability rate at the passage 20 (17.2 ± 8.87%). There was no change in karyotype and nuclear fragmentation was low in all cellular passages studied. With the scanning electron analysis was possible the characterization of AFCs and UCCs in suspension. The three cellular types of cells presented different shapes and characteristics on the surface. The results demonstrate that bovine AFCs and UCCs can be isolated, cultured in vitro and cryopreserved in 10% DMSO, not causing damage to DNA and chromosomes. The UCCs were more resistant than AFCs in all aspects.  相似文献   

Foliar and tuber assessment of late blight (Phytophthora infestans (Mont.) de Bary) reaction in cultivated potato (Solanum tuberosum L.)     

D. S. Douches  W. W. Kirk  M. A. Bertram  J. J. Coombs  B. A. Niemira 《Potato Research》2002,45(2-4):215-224

Summary Host plant resistance is an important component to the management of potato late blight,Phytophthora infestans (Mont.) de Bary. Assessment of potato lines (Solanum tuberosum L.) with various levels of resistance toP. infestans (US8, A2 genotype) were evaluated in field trials, greenhouse controlled environment chambers and inoculated tuber reactions. Five lines (AWN86514-2, B0692-4, B0718-3, Jacqueline Lee, and B0288-17) with strong foliar resistance to late blight were identified in these inoculated field trials. Greenhouse controlled environment chamber studies allowed resistant and susceptible lines to be distinguished, but the 1998 greenhouse results did not correlate well with field data. Four lines (A084275-3, Bzura, MSG007-1, and MSG297-4RD) evaluated by a digital image analysis technique demonstrated tuber resistance based upon average reflective index values in the inoculated tuber studies. Tuber resistance did not correlate with field foliar resistance. Based upon these results, field assessment of foliar reaction toP. infestans provides the best measure for assessing late blight resistance in potato. Tuber resistance to late blight can be identified among lines with varying levels of foliar resistance.  相似文献   

The Mirab Process: Earlier Analyses in Context     

I. G. Bertram  R. F. Watters 《Asia Pacific viewpoint》1986,27(1):47-59

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