排序方式： 共有37条查询结果，搜索用时 15 毫秒
31.
Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata 总被引：1，自引：1，他引：0
Bouraoui Z Banni M Ghedira J Clerandeau C Guerbej H Narbonne JF Boussetta H 《Fish physiology and biochemistry》2008,34(3):201-207
This research was designed to study Sparus aurata (sea bream) biotransformation and detoxification responses to acute exposure to cadmium (Cd). Sexually immature gilthead
sea bream were treated by intraperitoneal injection of Cd chloride (200 μg kg−1) for 6, 12, 24 and 48 h. Cd accumulation was quantified in sea bream liver by graphite furnace atomic absorption spectroscopy
after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD)
activity as phase I biotransformation parameter, (2) liver glutathione-S-transferase (GST) activity as a phase II conjugation
enzyme and metallothionein (MT) content as specific response to Cd contamination. Cd bioaccumulation in the liver resulted
in an increasing uptake up to 10.3 μg g−1 wet weight after 48 h of exposure. EROD showed a significant activation only after 6 h exposure and a return to control levels
after 12 h. GST revealed significant activation starting from 12 h exposure. MT accumulation in liver showed the same behavior
as GST activation. 相似文献
32.
Noureddine Baaka Manel Ben Ticha Wafa Haddar Maria Teresa Pessoa Amorim Mohamed Farouk Mhenni 《Fibers and Polymers》2018,19(2):307-312
The desire to offer clothes protective properties against the harmful UV rays continues to attract the interest of researchers. Several works have been elaborated in this topic and have proved that the clothes do not protect sufficiently against the solar ultraviolet radiation. Their protectiveness depends on the fiber type, the fabric composition, the fabric construction and the dyeing method. This research presents a novel approach which target the improvement of the textile protective properties against UV rays by their dyeing with grape pomace aqueous extract. These properties were tested in combination with several fabrics (natural and synthetic) and mordants (potassium alum and ferrous sulphate). Through this study, it has been shown that the UV-protective properties of the several dyed fibers were upgraded after dyeing with the grape pomace extract, and is intensely relying on the types of fibers and mordants. 相似文献
33.
Manuel Alvarez-Rodriguez Maria Ntzouni Dominic Wright Kabirul Islam Khan Manel López-Béjar Cristina A. Martinez Heriberto Rodriguez-Martinez 《Reproduction in domestic animals》2020,55(3):293-300
The avian seminal fluid (SF) is a protein-rich fluid, derived from the testis, the rudimentary epididymis and, finally, from the cloacal gland. The SF interacts with spermatozoa and the inner cell lining of the female genital tract, to modulate sperm functions and female immune responsiveness. Its complex proteome might either be free or linked to extracellular vesicles (EVs) as it is the case in mammals, where EVs depict the tetraspanin CD9; and where those EVs derived from the epididymis (epididymosomes) also present the receptor CD44. In the present study, sperm-free SF from Red Jungle Fowl, White Leghorn and an advanced intercross (AIL, 12th generation) were studied using flow cytometry of the membrane marker tetraspanin CD9, Western blotting of the membrane receptor CD44 and electron microscopy in non-enriched (whole SF) or enriched fractions obtained by precipitation using a commercial kit (Total Exosome Precipitation Solution). Neither CD9- nor CD44 could be detected, and the ultrastructure confirmed the relative absence of EVs, raising the possibility that avian SF interacts differently with the female genitalia as compared to the seminal plasma of mammals. 相似文献
34.
The aim behind the present research is to develop an enzymatic treatment for olive mill wastewater (OMW) to release high amounts of simple phenolics having high antioxidant value. OMW was hydrolyzed by a mixed enzyme preparation rich in β-glucosidase produced by Aspergillus niger . This research shows that A. niger β-glucosidase played a major role in the release of simple phenolic compounds from OMW. These compounds were recovered by ethyl acetate extraction and identified by HPLC and LC-MS. The main identified phenolic compound is hydroxytyrosol. The results of enzymatic hydrolysis of OMW under optimum conditions indicated a maximum hydroxytyrosol concentration of 2.9 g L(-1) compared to 0.015 g L(-1) contained in the control (test without added enzyme). The above results prove that OMW is a potential substrate for producing hydroxytyrosol through enzymatic hydrolysis of its glycosides. 相似文献
35.
Esteller M Fraga MF Paz MF Campo E Colomer D Novo FJ Calasanz MJ Galm O Guo M Benitez J Herman JG 《Science (New York, N.Y.)》2002,297(5588):1807-8; discussion 1807-8
36.
M. Banni Z. Bouraoui J. Ghedira C. Clerandeau H. Guerbej J. F. Narbonne H. Boussetta 《Fish physiology and biochemistry》2009,35(2):293-299
In the present study biotransformation and detoxification responses to acute exposure to the polycyclic aromatic hydrocarbons
benzo[a]pyrene (B[a]P) were investigated in the liver of Sparus aurata (sea bream). Sexually immature gilthead sea bream were treated by intraperitoneal injection of B[a]P (20 mg kg−1) for 6, 12, 24, and 48 h. B[a]P accumulation was quantified in sea bream liver by mean of gas phase chromatography (GPC-MS)
after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity, as a phase I biotransformation parameter; (2) liver glutathione S-transferase (GST) activity as a phase II conjugation enzyme. DNA damage was assessed over time using the single-cell gel
electrophoresis comet assay. B[a]P bioaccumulation in the liver resulted in a biphasic curve with an increasing uptake up
to 5.55 ± 0.67 μg g−1 dry weight after only 6 h exposure and 4.67 ± 0.68 μg g−1 dry weight after 48 h exposure. EROD activity showed a nonsymmetrical bell-shaped kinetic with a maximum at 24 h and lower
but significant activities at 12 and 48 h with respect to control animals. Hepatic GST activities were only significant after
48 h exposure. Comet assay showed an increase in liver cells DNA damage with a maximum after 48 h exposure reaching up to
12.17 %DNA in the tail. 相似文献
37.
A health evaluation in a colony of captive collared peccaries (Tayassu tajacu) in the Eastern Amazon
Mayor P Le Pendu Y Guimarães DA da Silva JV Tavares HL Tello M Pereira W López-Béjar M Jori F 《Research in veterinary science》2006,81(2):246-253
This study pretends to determine baseline data on the health and mortality of a colony of captive collared peccaries in the Eastern Amazon (Belém, State of Pará, Brazil) during a 65-months survey. Thirty-nine out of 166 animals (23.5%) died and were examined post-mortem. Monthly mortality averaged 1.2%. The highest mortality rate was observed in newborns (74.4%). Abandonment by the mother and aggression were responsible for 24.1% and 13.8% of the total newborn deaths, respectively. Most frequent causes of non-neonatal death were food poisoning (50.0%) due to an episode of accidental bitter cassava leaves ingestion and traumatism due to aggressions between animals (10.0%). Results from serology for different infectious diseases showed that 4.9% (2/41) collared peccaries had antibodies against Brucella spp. and 9.8% (4/41) animals had antibodies to two different Leptospira spp. serovars, butembo and autumnalis. This is the first survey of morbidity and mortality in captive collared peccaries in the Amazon region. 相似文献