Composition, acidity and rennet-coagulation properties of early- and late-lactation milks from Italian Friesian cows     

Summer A  Formaggioni P  Malacarne M  Sandri S  Mariani P 《Veterinary research communications》2003,27(Z1):269-272

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Effects of milk storage temperature at the herd on cheesemaking losses in the manufacture of Parmigiano-Reggiano cheese     

Franceschi P  Sandri S  Pecorari M  Vecchia P  Sinisi F  Mariani P 《Veterinary research communications》2008,32(Z1):S339-S341

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Identification of a universal Group B streptococcus vaccine by multiple genome screen   总被引：2，自引：0，他引：2  

Maione D  Margarit I  Rinaudo CD  Masignani V  Mora M  Scarselli M  Tettelin H  Brettoni C  Iacobini ET  Rosini R  D'Agostino N  Miorin L  Buccato S  Mariani M  Galli G  Nogarotto R  Nardi-Dei V  Nardi Dei V  Vegni F  Fraser C  Mancuso G  Teti G  Madoff LC  Paoletti LC  Rappuoli R  Kasper DL  Telford JL  Grandi G 《Science (New York, N.Y.)》2005,309(5731):148-150

Group B Streptococcus (GBS) is a multiserotype bacterial pathogen representing a major cause of life-threatening infections in newborns. To develop a broadly protective vaccine, we analyzed the genome sequences of eight GBS isolates and cloned and tested 312 surface proteins as vaccines. Four proteins elicited protection in mice, and their combination proved highly protective against a large panel of strains, including all circulating serotypes. Protection also correlated with antigen accessibility on the bacterial surface and with the induction of opsonophagocytic antibodies. Multigenome analysis and screening described here represent a powerful strategy for identifying potential vaccine candidates against highly variable pathogens.  相似文献   

Association between anesthesia duration and outcome in dogs with surgically treated acute severe spinal cord injury caused by thoracolumbar intervertebral disk herniation     

Joe Fenn  Hongyu Ru  Nick D. Jeffery  Sarah Moore  Andrea Tipold  Franz J. Soebbeler  Adriano Wang-Leandro  Christopher L. Mariani  Peter J. Early  Karen R. Muñana  Natasha J. Olby 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(4):1507-1513

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Insulin binding to isolated hepatocytes of Atlantic salmon and rainbow trout     

Erika M. Plisetskaya  Elena Fabbri  Thomas W. Moon  Joaquim Gutiérrez  Celestina Ottolenghi 《Fish physiology and biochemistry》1993,11(1-6):401-409

The questions addressed in this study were: 1) whether insulin added to the incubation medium can down-regulate ¹²⁵I insulin binding to isolated hepatocytes of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss); 2) whether quantitative assessment of insulin processing can be made on isolated fish liver cells; 3) how ambient temperatures can affect insulin binding, and down-regulation of insulin receptors. After isolation and a short (up to 4h) “metabolic recovery period”, liver cells were used either directly in ¹²⁵I insulin binding assay or first preincubated for 18h at 4°C or for 3h at 15°C, with or without mammalian or salmon insulin in concentrations ranging from 1 to 1000 nM. Preincubation at 15°C, decreased binding capacity (number of binding sites per liver cell) in all five independent hepatocyte preparations treated with 1000 nM insulin and in four out of five preparations treated with 100 nM insulin. At 4°C insulin binding sites were down-regulated in less than 50% of all hepatocyte preparations and only in the presence of 1000 nM insulin. Differential quantitive assessment was made of a) intact free insulin; b) insulin degraded; c) intact insulin bound to the cell membrane; d) internalized but degraded insulin, and e) intact insulin internalized by liver cells. Hepatocytes preincubated with 100 – 1000 nM insulin at 15°C bound and internalized less ¹²⁵I insulin. We hypothesize that in vivo, at water temperatures of 15°C and higher, extreme physiological levels of plasma insulin may regulate the numbers of insulin receptors in the salmonid liver. In contrast, in fish inhabiting cold waters the regulation of insulin receptors by circulating plasma insulin seems to be of little physiological importance. Presented in part at the Western Regional Conference on Comparative Endrocrinology, Tempe, Arizona, U.S.A., 1991 and at the Meeting of Italian Society of Experimental Biology, Sorrento, Italy, 1991. Supported by grants from NSF of the USA#DCB 8915935 to E.M.P., NSERC of Canada OPGA 6944 to T.W.M., North Atlantic Treaty Organization (NATO) grant #0926/87 to C.O. and E.M.P., and CYCIT grant of Spain to J.G.  相似文献   

MAGNETIC RESONANCE IMAGING OF SPONGY DEGENERATION OF THE CENTRAL NERVOUS SYSTEM IN A LABRADOR RETRIEVER     

Christopher L.  Mariani  DVM  Roger M.  Clemmons  DVM  PhD  John P.  Graham  MVB  MSc  Lynette A.  Phillips  DVM  PhD  Cheryl L.  Chrisman  DVM  MS  EDS 《Veterinary radiology & ultrasound》2001,42(4):285-290

A 7-month-old, neutered female Labrador Retriever was evaluated for tetraparesis and subtle cerebellar dysfunction. Clinical signs progressed over a period of 6 weeks to severe ataxia, hypermetria, intention tremors, and finally non-ambulatory tetraparesis. On magnetic resonance imaging of the brain there were large, bilaterally symmetrical, ovoid lesions in the region of the deep cerebellar nuclei that were hyperintense on T2-weighted and proton density images and hypointense on T1-weighted images. There were similar but smaller bilaterally symmetrical lesions present within the thalamus. Euthanasia was performed and lesions consistent with the previously described spongy degeneration of Labrador Retrievers were identified. This disease and its relation to similar human heritable leukodystrophies are discussed.  相似文献   

Evidence of Anti-Gliadin and Transglutaminase Antibodies in Sera of Dogs Affected by Lymphoplasmacytic Enteritis     

Vincenzetti  S.  Rossi  G.  Mariani  P.  Pengo  G.  Cammertoni  N.  Vita  A. 《Veterinary research communications》2006,30(1):219-221

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Teratoma in the cervical spinal cord of a dog     

Wong MA  Mariani CL  Powe JR  Clemmons RM 《Journal of the American Animal Hospital Association》2007,43(5):292-297

An 11-year-old, spayed female giant schnauzer was presented for evaluation of chronic, progressive tetraparesis. Diagnostic imaging was consistent with intervertebral disk protrusion, and surgical decompression and stabilization were performed. Postoperatively the dog did not improve, and further imaging suggested an intramedullary mass at the level of the sixth cervical vertebra. The dog was euthanized 7 days after surgery, and a teratoma was found postmortem.  相似文献   

Purification and Identification of αs1- and β-Caseins from Asses Milk     

Vincenzetti  S.  Polidori  P.  Salimei  E.  Mariani  P. L.  Allegri  S.  Cammertoni  N.  Quadrini  B.  Vita  A. 《Veterinary research communications》2015,29(2):211-213

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Histiocytic Sarcoma with Central Nervous System Involvement in Dogs: 19 Cases (2006–2012)          下载免费PDF全文

C.L. Mariani  M.K. Jennings  N.J. Olby  L.B. Borst  J.C. Brown Jr  I.D. Robertson  G.S. Seiler  E. MacKillop 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2015,29(2):607-613

Background

Reports of histiocytic sarcoma (HS) involving the central nervous system (CNS) are sparse and consist mainly of case reports describing 1–3 animals.

Objective

The objective of this study was to report the signalments, clinical signs, clinicopathologic and diagnostic imaging findings, treatment, and outcome of a series of dogs with HS and CNS involvement.

Animals

Nineteen dogs with HS examined at veterinary referral hospitals.

Methods

Retrospective case series. Medical records were reviewed and cases with a histopathological diagnosis of CNS HS were included in the study. Diagnostic imaging studies of the CNS were evaluated and histopathologic samples were reviewed to confirm the diagnosis.

Results

Retrievers and Pembroke Welsh Corgis were overrepresented in this cohort of dogs. Tumors involved the brain in 14 dogs and the spinal cord in 5. In 4 dogs, HS was part of a disseminated, multiorgan process whereas it appeared confined to the CNS in 15 dogs. Diagnostic imaging had variable appearances although extraaxial masses predominated in the brain. There was meningeal enhancement in all dogs that was often profound and remote from the primary mass lesion. Pleocytosis was present in all dogs with CSF evaluation. Median survival was 3 days.

Conclusions and Clinical Importance

Breed predispositions appear to vary from reports of HS in other organ systems. Some unique imaging and clinicopathologic characteristics, particularly brain herniation, profound meningeal enhancement, and pleocytosis in combination with 1 or more mass lesions, might help to differentiate this neoplasm from others involving the CNS, although this requires further study.  相似文献