Cerebrospinal fluid lactate concentrations in dogs with seizure disorders     

Christopher L. Mariani  Carolyn J. Nye  Laura Ruterbories  Debra A. Tokarz  Lauren Green  Jeanie Lau  Natalia Zidan  Peter J. Early  Karen R. Muñana  Natasha J. Olby  Chun-Sheng Lee  Julien Guevar 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2020,34(6):2562-2570

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Increasing 2n gamete production in diploid alfalfa by cycles of phenotypic recurrent selection   总被引：3，自引：0，他引：3  

O. Calderini  A. Mariani 《Euphytica》1997,93(1):113-118

Two cycles of selection (3rd and 4th) were carried out in a diploid population of alfalfa to improve the frequency of 2n gametes. All the plants produced unreduced gametes, except one in the 2n egg population. The average seed set, used as a measure of 2n gametes production, increased significantly in the 2n pollen selection. Conversely, the increase was not significant in the case of 2n eggs. The improvement in 2n pollen production was confirmed cytologically. These results, like those of the first two cycles of selection, suggest that 2n pollen production is controlled by few major genes while 2n egg production is controlled by one recessive major gene and influenced by minor genes. The mutants selected have been utilized for producing heterotic tetraploid progenies through Bilateral Sexual Polyploidization (BSP).  相似文献   

Perianesthetic morbidity and mortality in dogs undergoing cervical and thoracolumbar spinal surgery     

Lysa P Posner  Christopher L Mariani  Cliff Swanson  Makoto Asakawa  Nigel Campbell  Adam S King 《Veterinary anaesthesia and analgesia》2014,41(2):137-144

ObjectiveTo evaluate and compare perioperative morbidity and mortality in dogs undergoing cervical and thoracolumbar spinal surgery.Study designProspective case series.Animals157 dogs undergoing cervical or thoracolumbar spinal surgery.MethodsData were collected sequentially on canine cases presented from the Neurology Section of the North Carolina State University Veterinary Teaching Hospital for anesthesia and surgery for cervical spinal cord disease. Simultaneously, data were collected on all thoracolumbar spinal surgery cases during the same time period. Data included signalment, drugs administered, surgical approach, disease process, cardiac arrhythmias during anesthesia, and outcome.ResultsData were collected from 164 surgical events in 157 dogs. There were 52 cervical approaches; four dorsal and 48 ventral. All thoracolumbar surgeries were approached dorsolaterally. Four dogs 4/52 (7.6%) undergoing a cervical approach did not survive to discharge. Two dogs (2/8; 25%) underwent atlanto-axial (AA) stabilization and suffered cardiovascular arrest and two dogs (2/38; 5.2%) undergoing cervical ventral slot procedures were euthanized following anesthesia and surgery due to signs of aspiration pneumonia. All dogs undergoing thoracolumbar surgery survived until discharge (112/112). Mortality in dogs undergoing cervical spinal surgery was greater compared with dogs undergoing thoracolumbar spinal surgery (p = 0.009), however, in dogs undergoing decompressive disc surgery, intraoperative death rates were not different between dogs undergoing a cervical compared with thoracolumbar approaches (p = 0.32) nor was there a significant difference in overall mortality (p = 0.07).Conclusion and clinical relevanceOverall, dogs undergoing cervical spinal surgery were less likely to survive until discharge compared with dogs undergoing thoracolumbar spinal surgery. Mortality in dogs undergoing cervical intervertebral disc decompression surgery was no different than for dogs undergoing thoracolumbar intervertebral disc decompression surgery. However, dogs undergoing cervical intervertebral disc decompression surgery should be considered at risk for aspiration pneumonia.  相似文献   

Analysis of Organic Micropollutants in Sediment Samples of the Venice Lagoon, Italy     

E. Fattore  E. Benfenati  G. Mariani  E. Cools  G. Vezzoli  R. Fanelli 《Water, air, and soil pollution》1997,99(1-4):237-244

Surface sediment samples were collected at six locations of the Lagoon of Venice reflecting potential different contamination sources and representative of different hydrological situations. Analysis of polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/PCDFs), polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), linear alkylbenzenes (LABS) and coprostanol have been carried out by gas chromatographymass spectrometry (GC-MS) to assess the influence of various pollution sources: urban, industrial and combustion processes. PAHs, PCBs, coprostanol, and LABS showed the highest levels in the sample collected within the city of Venice (Canal Grande) indicating a very heavy contamination due to combustion sources and to the domestic waste waters directly entering the canals of the city. The highest levels of PCDD/PCDFs were found in samples collected near the industrial area of Porto Marghera. The investigation on the PCDD/PCDFs homologue profiles suggested the presence of two different sources for these pollutants, one due to combustion processes and another one due to the chemical processes of Porto Marghera.  相似文献   

Protein fraction characterization of sheep milk from the Comisana breed     

Vincenzetti  S.  Polidori  P.  Mariani  P.  Vita  A. 《Veterinary research communications》2008,32(1):179-181

Veterinary Research Communications -  相似文献   

Exploring the natural variation for reproductive thermotolerance in wild tomato species     

Nicky Driedonks  Mieke Wolters-Arts  Heidrun Huber  Gert-Jan de Boer  Wim Vriezen  Celestina Mariani  Ivo Rieu 《Euphytica》2018,214(4):67

Climate change has become a serious threat for crop productivity worldwide. The increased frequency of heat waves strongly affects reproductive success and thus yield for many crop species, implying that breeding for thermotolerant cultivars is critical for food security. Insight into the genetic architecture of reproductive heat tolerance contributes to our fundamental understanding of the stress sensitivity of this process and at the same time may have applied value. In the case of tomato (Solanum lycopersicum), germplasm screenings for thermotolerance have often used yield as the main measured trait. However, due to the complex nature of yield and the relatively narrow genetic variation present in the cultivated germplasm screened, there has been limited progress in understanding the genetic basis of reproductive heat tolerance. Extending the screening to wild accessions of related species that cover a range of climatic conditions might be an effective approach to find novel, more tolerant genetic resources. The purpose of this study was to provide insight into the sensitivity of individual reproductive key traits (i.e. the number of pollen per flower, pollen viability and style protrusion) to heat-wave like long-term mild heat (LTMH), and determine the extent to which genetic variation exists for these traits among wild tomato species. We found that these traits were highly variable among the screened accessions. Although no overall thermotolerant species were identified, several S. pimpinellifolium individuals outperformed the best performing cultivar in terms of pollen viability under LTMH. Furthermore, we reveal that there has been local adaptation of reproductive heat tolerance, as accessions from lower elevations and higher annual temperature are more likely to show high pollen viability under LTMH.  相似文献   

Characterization of immune cell infiltration into canine intracranial meningiomas     

Boozer LB  Davis TW  Borst LB  Zseltvay KM  Olby NJ  Mariani CL 《Veterinary pathology》2012,49(5):784-795

Meningiomas are the most common intracranial tumors in dogs. A variety of inflammatory cells have been shown to invade these tumors in people, but little is known about interactions between the immune system and naturally occurring brain tumors in dogs. The purpose of this study was to investigate the presence of a variety of immune cell subsets within canine intracranial meningiomas. Twenty-three formalin-fixed, paraffin-embedded tumor samples were evaluated using immunohistochemistry with antibodies specific for CD3, CD79a, CD18, CD11d (αD), CD45RA, forkhead box P3, and Toll-like receptors 4 and 9. Immune cell infiltration was evident in all samples, with a predominance of CD3(+) T cells. Large numbers of CD18(+) microglia and macrophages were noted surrounding and infiltrating the tumors, and a subset of these cells within the tumor appeared to be CD11d(+). Scattered macrophages at the tumor-brain interface were TLR4(+) and TLR9(+). Rare CD79a(+) B cells were noted in only a small subset of tumors. Lesser numbers of lymphocytes that were CD11d(+), CD45RA(+), or FoxP3(+) were noted in a number of the meningiomas. Although the function of these cells is not yet clear, work in other species suggests that evaluation of this immune cell infiltrate may provide important prognostic information and may be useful in the design of novel therapies.  相似文献   

Clinical signs, underlying cause, and outcome in cats with seizures: 17 cases (1997-2002)     

Barnes HL  Chrisman CL  Mariani CL  Sims M  Alleman AR 《Journal of the American Veterinary Medical Association》2004,225(11):1723-1726

OBJECTIVE: To determine clinical signs, results of diagnostic testing, underlying cause, and outcome in cats with seizures. DESIGN: Retrospective study. ANIMALS: 17 cats with seizures. PROCEDURE: Only those cats in which an underlying metabolic abnormality causing the seizures had been identified, diagnostic imaging of the brain and CSF analysis had been done, or a necropsy had been performed were included. Seizures were classified as being a result of metabolic disease, symptomatic epilepsy (ie, epilepsy resulting from a structural lesion of the brain), or probably symptomatic epilepsy (ie, epilepsy without any extracranial or identifiable intracranial disease that is not suspected to be genetic in origin). RESULTS: 3 cats had seizures associated with an underlying metabolic disease (hepatic encephalopathy), 7 had symptomatic epilepsy (3 with neoplasia and 4 with meningoencephalitis), and 7 had probably symptomatic epilepsy. Six of the 7 cats with symptomatic epilepsy died or were euthanatized within 3 months after the diagnosis was made, whereas 6 of the 7 cats with probably symptomatic epilepsy survived for at least 12 months after the diagnosis was made. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that cats with probably symptomatic epilepsy may have a good long-term prognosis.  相似文献   

Purification and identification of alphas1- and beta-caseins from asses milk     

Vincenzetti S  Polidori P  Salimei E  Mariani PL  Allegri S  Cammertoni N  Quadrini B  Vita A 《Veterinary research communications》2005,29(Z2):211-213

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Cerebral phaeohyphomycosis caused by Cladosporium spp. in two domestic shorthair cats     

Mariani CL  Platt SR  Scase TJ  Howerth EW  Chrisman CL  Clemmons RM 《Journal of the American Animal Hospital Association》2002,38(3):225-230

Two domestic shorthair cats presented for clinical signs related to multifocal central nervous system dysfunction. Both cats had signs of vestibular system involvement and anisocoria, and one had generalized seizure activity. Cerebrospinal fluid analysis revealed a neutrophilic pleocytosis with protein elevation in one cat and pyogranulomatous inflammation in the second. Electroencephalography and brain-stem auditory-evoked potentials in the first cat confirmed cerebral cortical and brain-stem involvement. Euthanasia was performed in both cats, and postmortem diagnoses of phaeohyphomycosis secondary to Cladosporium spp. were made based on histopathology and fungal culture in both cats.  相似文献