Both anti-oxidation and lipid-carbohydrate conversion enhancements are involved in priming-improved emergence of Echinacea purpurea seeds that differ in size     

K.Y. Chiu  S.J. Chuang  J.M. Sung 《Scientia Horticulturae》2006

This study evaluated the effects of priming on emergence responses of purple coneflower (Echinacea purpurea L. Moench) seeds. The seeds that differ in seed size were either primed with moistened vermiculite (solid matrix priming) or primed in non-aerated −0.5 MPa polyethylene glycol 6000 solution at 25 °C for 6 days (osmopriming), followed by air-drying to their initial moisture level. The tetrazolium staining tests indicated that both large and small seeds were biochemically viable. No notable difference in germination percentage was found between large and small seeds. However, extensive cavity was visible in portions of small seeds in comparison with large seeds. Large seeds accumulated more antioxidants and had greater activities of anti-oxidative enzymes than small seeds. They also had greater isocitrate lyase and malate synthase activities than small seeds. As a result, large seeds had higher emergence percentage and faster emergence speed as compared to that of small seeds. Both solid matrix priming and osmopriming increased emergence percentage and shortened mean emergence time of purple coneflower seeds by increasing the activities of anti-oxidative enzymes and decreasing the levels of malondialdehyde and total peroxide accumulation. Moreover, priming also enhanced the anti-oxidative activities of treated seeds. The activities of isocitrate lyase and malate synthase were also increased in primed seeds. The enhanced anti-oxidation and lipid-carbohydrate conversion activities might explain in part why primed purple coneflower seeds emerged better than non-primed seeds.  相似文献   

北京菜豆(Phaseolus Vulgaris)上的一种新病毒—和性黄色花叶病毒     

刘亮  裘维藩 《植物病理学报》1987,17(2):96-101

 从北京郊区患有类似花叶病害的菜豆株上分离到一种线条状病毒(长约700至750nm)并于1983至1986年间加以研究。在温室内接种可以侵染菜豆、蚕豆、豌豆、大豆、决明、苋色藜及昆诺藜而不能侵染被接种的任何茄科植物.同标准的菜豆普遍花叶病毒(BCMV)相比,它不侵染茄科植物例如黄花烟(Nicotiana rustica)和矮牵牛(Petunia hybrida)在菜豆叶上也不产生坏死枯斑.此外它能侵染蚕豆、大豆、豌豆及决明而BCMV则不能.BCMV能侵染豇豆而这一分离物则不能.此分离物的物理性状为:钝化温度=56-580℃(十分钟),硫释限点=10^-3至10^-4,180℃下存治期为3天,A260/280=1.12.菜豆受侵叶组织易用光学显微镜观察到内含体包括一种片层叠合体.在电镜下超薄切片中可以看到风轮状体.极易用汁液摩接及用桃蚜(Myzus persic(接种.此病毒的衣壳蛋白亚基的分子量经测定为32,000道尔顿.此病毒与下列病毒即菜豆普通花叶病毒,菜豆黄色花叶病毒,黑眼豇豆花叶病毒,豌豆种传花叶病毒,三叶草黄脉病毒,莴苣花叶病毒及甜菜花叶病毒的抗血清均无反应.由于它在菜豆的"一窝猴"品种的叶片上产生沿脉黄色小点,因此认为这是一种新的独立的病毒,称之为菜豆和性黄色花叶病毒.  相似文献   

小麦丛矮病研究之二关于小麦丛矮病的病原病毒问题     

杨莉  林志亮  梅汝鸿  蔡祝南  裘维蕃  傅仓生 《植物保护学报》1979,6(1):17-23

在田间的小麦丛矮病非典型病株申常常分离到北方禾谷花叶病毒(NCMV)及玉米粗缩病毒(MRDV)两种病原物。单独用前者接种,仅表现小麦丛矮症,单独用后者接种,仅表现小麦蓝矮症,即植株矮化、暗绿、分蘖减少,叶坚挺。两者混合侵染时,往往表现两者的混合症状,但以何种病毒占优势而倾向于优势病毒的症状。典型丛矮症植株中电镜检出弹状病毒粒体,40—70×270—400nm尺度与日本报导的稍异。典型丛矮病株中不存在玉米粗缩病毒的粒体。小麦丛矮病毒很可能是北方禾谷花叶病毒的一种地方性株系。  相似文献   

Development of an enzyme-linked immunosorbent assay for detecting infectious bursal disease virus (IBDV) infection based on the VP3 structural protein   总被引：1，自引：0，他引：1  

Wang MY  Hu HL  Suen SY  Chiu FY  Shien JH  Lai SY 《Veterinary microbiology》2008,131(3-4):229-236

Infectious bursal disease virus (IBDV) causes a contagious immunosuppressive disease in chickens. The aim of the present study is to develop an enzyme-linked immunosorbent assay (ELISA) using the expressed VP2 or VP3 protein of IBDV as the coating antigen for detecting antibodies to IBDV. Experimental results were compared with virus neutralization assay and a commercial-available ELISA. These assays were used to examine the sera from farm chickens and chickens vaccinated experimentally. The VP3-based ELISA had a higher correlation coefficient (R(2)) of 0.812 with a commercial ELISA kit at a serum dilution of 1:500 than that of VP2-based ELISA (R(2)) of 0.671. The relative sensitivity between virus neutralization and VP2-ELISA and VP3-ELISA was 96% (251/262) and 100% (262/262), respectively, and that between virus neutralization and a commercial ELISA was 99% (257/261). Additionally, compared with virus neutralization assay, the reference technique for diagnosing IBDV, VP3-based ELISA had an agreement value of 99%, superior to that of VP2-based ELISA (95%) or the commercial kit (89%). These results revealed that the capability of either VP2-ELISA or VP3-ELISA in detecting the field chicken sera was comparable to the commercial one, which is generally used to replace the virus neutralization assay. However, the preparation of VP3 is derived from an Escherichia coli expression system with a high yield and purification efficiency by Ni(2+)-NTA gels, which is more favorable to the insect cell-derived particles formed by VP2. Therefore, VP3-ELISA could be developed as an efficient and low cost diagnostic method for IBDV infection in field chickens.  相似文献   

柑桔苗黄型衰退病毒的分布概况和六种酸橙类砧木对它的反应   总被引：4，自引：0，他引：4  

赵学源  蒋元晖  张权炳  邱柱石  苏维芳 《植物病理学报》1979,9(1):61-64

 用尤力克柠檬和葡萄柚作指示植物,鉴定广西、广东、湖南、江西、浙江、四川等6个省(区)的873株柑桔中有691株受苗黄型衰退病毒感染,说明这种病毒的分布相当普遍。  相似文献   

棉苗黑斑病的发生条件及其防治途径     

李君彦  仇元 《植物保护学报》1963,2(2):195-204

棉苗黑斑病的致病菌在关中棉区初步认为是 Alternaria tenuis Nees 与棉成株期叶片上的致病菌不同。病菌孢子借气流传布。种子带菌率虽然很高,但诱发苗病率却很低,因此,种子消毒或采用轮栽来控制这个病害,是不能达到预期效果的。病害发生与棉苗受冻、强风对棉苗的伤害以及棉苗阶段发育有密切的关系。各年发病期有早有晚,但以真叶出现时为易感阶段。本文阐述了棉苗生育阶段、气候条件与发病的关系,为预测病害发生的物候与气象参考条件提供了资料。棉株伤害与发病有关系,建议在生产上适当降低播种量,提高播种质量,适期早疏苗、早间苗;以减轻由于伤损而导致病害侵染。品种与病害发生的关系不显著。试验证明,使用波尔多液加1059于降温前喷射效果较好。  相似文献   

大白菜窖藏中一些腐烂细菌的研究     

裘维蕃  狄原渤  周毓瑶  司凤举 《植物病理学报》1964,7(2):127-134

 窖藏白菜腐烂是由许多细菌造成的。最重要的是Erwinia aroideae和E.phytophthora以及被认为是腐生菌的三种芽孢杆菌Bacillus pumilus,B.poly-myxa和B.subtilis。试验证明在一定条件下后者能引致明显的腐烂。  相似文献   

Human endothelial cell growth factor: cloning, nucleotide sequence, and chromosome localization   总被引：78，自引：0，他引：78  

M Jaye  R Howk  W Burgess  G A Ricca  I M Chiu  M W Ravera  S J O'Brien  W S Modi  T Maciag  W N Drohan 《Science (New York, N.Y.)》1986,233(4763):541-545

Several of the endothelial cell polypeptide mitogens that have been described probably play a role in blood vessel homeostasis. Two overlapping complementary DNA clones encoding human endothelial cell growth factor (ECGF) were isolated from a human brain stem complementary DNA library. Southern blot analysis suggested that there is a single copy of the ECGF gene and that it maps to human chromosome 5 at bands 5q31.3 to 33.2 A 4.8-kilobase messenger RNA was present in human brain stem messenger RNA. The complete amino acid sequence of human ECGF was deduced from the nucleic acid sequence of these clones; it encompasses all the well-characterized acidic endothelial cell polypeptide mitogens described by several laboratories. The ECGF-encoding open reading frame is flanked by translation stop codons and provides no signal peptide or internal hydrophobic domain for the secretion of ECGF. This property is shared by human interleukin-1, which is approximately 30 percent homologous to ECGF.  相似文献   

Human sperm binding is mediated by the sialyl-Lewis(x) oligosaccharide on the zona pellucida     

Pang PC  Chiu PC  Lee CL  Chang LY  Panico M  Morris HR  Haslam SM  Khoo KH  Clark GF  Yeung WS  Dell A 《Science (New York, N.Y.)》2011,333(6050):1761-1764

Human fertilization begins when spermatozoa bind to the extracellular matrix coating of the oocyte, known as the zona pellucida (ZP). One spermatozoan then penetrates this matrix and fuses with the egg cell, generating a zygote. Although carbohydrate sequences on the ZP have been implicated in sperm binding, the nature of the ligand was unknown. Here, ultrasensitive mass spectrometric analyses revealed that the sialyl-Lewis(x) sequence [NeuAcα2-3Galβ1-4(Fucα1-3)GlcNAc], a well-known selectin ligand, is the most abundant terminal sequence on the N- and O-glycans of human ZP. Sperm-ZP binding was largely inhibited by glycoconjugates terminated with sialyl-Lewis(x) sequences or by antibodies directed against this sequence. Thus, the sialyl-Lewis(x) sequence represents the major carbohydrate ligand for human sperm-egg binding.  相似文献   

Interleukin-1 costimulatory activity on the interleukin-2 promoter via AP-1   总被引：37，自引：0，他引：37  

K Muegge  T M Williams  J Kant  M Karin  R Chiu  A Schmidt  U Siebenlist  H A Young  S K Durum 《Science (New York, N.Y.)》1989,246(4927):249-251

  相似文献