Purification, cloning, and identification of two thaumatin-like protein isoforms in jelly fig (Ficus awkeotsang) Achenes     

Chua AC  Chou WM  Chyan CL  Tzen JT 《Journal of agricultural and food chemistry》2007,55(18):7602-7608

Jelly curd used for a popular summer drink in Taiwan is prepared by extracting the pericarpial portion of jelly fig (Ficus awkeotsang Makino) achenes. The two most abundant proteins found in jelly curd have been identified as a pectin methylesterase and a chitinase. A method was developed to purify the next abundant protein by 40% ammonium sulfate precipitation and flowing through Mono Q chromatography. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses, the purified protein migrated as a polypeptide of 20 kDa in the absence of beta-mercaptoethanol but split into a minor polypeptide of 20 kDa and a major polypeptide of 27 kDa in the presence of this reducing agent. Two cDNA fragments encoding precursor polypeptides of two putative thaumatin-like protein isoforms were obtained by polymerase chain reaction cloning and subsequently overexpressed in Escherichia coli to generate recombinant proteins for antibody preparations. Immunological detection and mass spectrometric analyses indicated that the two split polypeptides were thaumatin-like protein isoforms encoded by the two cloned cDNA fragments.  相似文献   

甘肃黑猪血浆蛋白多态性与初生重、早期日增重关系的研究   总被引：1，自引：0，他引：1  

王彦芳  刘丑生  门正明 《畜牧与兽医》2002,34(7):3-5

采用聚丙烯酰胺凝胶电泳 (PAGE)对 37头甘肃黑猪合成系猪的转铁蛋白 (Tf)、前白蛋白 (Pa)、脂酶 (Es)、淀粉酶 1 (Am1 )、淀粉酶 2(Am2 ) 5个蛋白质基因座的多态性进行了检测。并运用通用线性模型分析了这 5个蛋白质基因座的共 1 7种基因型对初生重、 45日龄内平均日增重的相关效应。结果表明Am1AA型具有显著提高初生重的效应 ,PaAA型具有显著提高 45日龄内平均日增重的效应。因此这 2个位点可望用于标记辅助选择。  相似文献   

Complete nucleotide sequences of S1 and N genes of infectious bronchitis virus isolated in Japan and Taiwan   总被引：6，自引：0，他引：6  

Shieh HK  Shien JH  Chou HY  Shimizu Y  Chen JN  Chang PC 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(5):555-558

The complete nucleotide sequences of the S1 and N genes of three Japanese and one Taiwanese field strains of IBV are reported. These Japanese strains were found to have S1 sequences most similar to those of Australian strains and N sequences most similar to those of North American strains. This result suggested that these Japanese strains might all be recombinant viruses derived from recombination of Australia- and North America-related viruses. Moreover, the S1 proteins of all these Japanese and Taiwanese strains exhibit only a limited sequence homology to strains of Massachusetts and Connecticut serotypes that have been commonly used as vaccine strains. This result high lightens the importance of development of vaccines based on the local strains of IBV.  相似文献   

Can fog contribute to the nutrition of Chamaecyparis obtusa var. formosana? Uptake of a fog solute tracer into foliage and transport to roots     

Lai IL  Schroeder WH  Wu JT  Kuo-Huang LL  Mohl C  Chou CH 《Tree physiology》2007,27(7):1001-1009

Yellow cypress (Chamaecyparis obtusa (Siebold & Zucc.) Endl. var. formosana (Hayata) Rehder) is the predominant tree species of Taiwan's nutrient-poor, mountain fog forests. Little is known about the potential contribution of solute uptake from fog to the overall nutrition of these trees. Shoots of yellow cypress seedlings were misted with artificial fog containing the tracer rubidium (Rb) in laboratory and field experiments to determine if there is solute uptake from the fog. After misting shoots for six weeks, substantial amounts of tracer were detected in unexposed roots by inductively coupled plasma mass spectroscopy bulk analysis. Possible routes of entry were examined by element imaging with energy dispersive X-ray analysis. Direct uptake of the tracer into leaves across the cuticle and epidermis was small, excluding this as the major uptake path. Accumulations of Rb were found on leaf surfaces along the edges of the leaves. The almost daily changes in fog coverage and air humidity may enhance the accumulation of fog solutes at leaf edges. Accumulation of Rb was also found in narrow clefts between opposite leaves and between the outermost and underlying alternating stacked leaves. The clefts provide a direct passage from the leaf surface to the space beneath the imbricate leaves and the underlying alternate leaves, possibly facilitating solute uptake from fog, which in turn may contribute to the nutrition of yellow cypress.  相似文献   

Discoloration induced by Raffaelea quercivora isolates in Quercus serrata logs and its relation to phylogeny: a comparison among isolates with and without the Japanese oak wilt incidence including outside of Japan     

Dai Kusumoto  Hayato Masuya  Toshihide Hirao  Hideaki Goto  Keiko Hamaguchi  Wen-I Chou  Wiwat Suasa-ard  Sawai Buranapanichpan  Sopon Uraichuen  Oraphan Kern-asa  Sunisa Sanguansub  Aumporn Panmongkol  Thu Pham Quang  Sih Kahono  Heddy Julistiono  Naoto Kamata 《Journal of Forest Research》2014,19(4):404-410

Japanese oak wilt (JOW) has been prevalent in Japan since the late 1980s. Infections of the fungus, Raffaelea quercivora Kubono et Shin. Ito, which is transmitted by an ambrosia beetle [Platypus quercivorus (Murayama)], can cause JOW. Although R. quercivora, P. quercivorus, and oak trees are distributed in other Asia–Pacific countries, the incidence of JOW has not been reported outside Japan. In this study, we collected R. quercivora isolates from 5 Asian countries, including Japan, and compared their ability to induce sapwood discoloration by inoculating Q. serrata logs. The tangential widths of the discoloration in sapwoods inoculated with non-Japanese isolates were equivalent to or greater than those of the 2 Japanese isolates. This indicates that a lack of JOW incidence outside Japan is not because of the lowered ability of R. quercivora to spread discoloration compared with the Japanese isolates. Statistical analyses of the relationship between discoloration and phylogeny based on DNA sequences of actin and chitin synthase showed that the discoloration width was independent of phylogenetic relatedness among the isolates. To discuss why the occurrence of JOW has not been reported outside Japan, further studies (e.g., on host susceptibility and P. quercivorus aggression) throughout Asia are needed.  相似文献   

Protective Role of Comfrey Leave Extracts on UV-induced Zebrafish Fin Damage     

Chien-Chung Cheng  Chi-Yuan Chou  Yao-Chin Chang  Hsuan-Wen Wang  Chi-Chung Wen  Yau-Hung Chen 《Journal of toxicologic pathology》2014,27(2):115-121

In zebrafish, UV exposure leads to fin malformation phenotypes including fin reduction or absence. The present study evaluated UV-protective activities of comfrey leaves extracts in a zebrafish model by recording fin morphological changes. Chemopreventive effects of comfrey leave extracts were evaluated using Kaplan-Meier analysis and Cox proportional hazards regression. The results showed that (1) the mean times of return to normal fin in the UV+comfrey (50 and 100 ppm) groups were 3.43 and 2.86 days and were quicker compared with that in the UV only group (4.21 days); (2) zebrafish fins in the UV+comfrey (50 and 100 ppm) groups were 2.05 and 3.25 times more likely to return to normal than those in the UV only group; and (3) comfrey leave extracts had UV-absorbance abilities and significantly reduced ROS production in UV-exposed zebrafish embryos, which may attenuate UV-mediated apoptosis. In conclusion, comfrey leaves extracts may have the potential to be developed as UV-protective agents to protect zebrafish embryos from UV-induced damage.  相似文献   

Myxovirus-like structures in a case of human chronic polymyositis   总被引：11，自引：0，他引：11  

S M Chou 《Science (New York, N.Y.)》1967,158(807):1453-1455

Intranuclear and intracytoplasmic aggregates of filaments with tubular structures and transverse striations occurred in muscle tissues biopsied from a patient with chronic polymyositis. The filamentous tubules bear a close resemblance to the incomplete form of myxovirus in which the envelope is missing. Three biopsies from the same patient, taken during a period of 1(1/2) years, all revealed these structures. This finding provides presumptive evidence that a chronic persistent viral infection may be involved in the pathogenesis of chronic polymyositis.  相似文献   

中国蛾蜡蝉科一新记录属一新种（同翅目：蜡蝉总科）     

王应伦  周尧  袁锋 《西北农林科技大学学报(自然科学版)》1994,22(3):41-43

本文记述了蛾蜡蝉科Ｆｌａｔｉｄａｅ国内一新记录属和一新种，模式标本保存在西北农业大学昆虫博物馆  相似文献   

Germline organization of the murine T cell receptor beta-chain genes   总被引：11，自引：0，他引：11  

H S Chou  C A Nelson  S A Godambe  D D Chaplin  D Y Loh 《Science (New York, N.Y.)》1987,238(4826):545-548

  相似文献   

Brain serotonin concentration: elevation following intraperitoneal administration of melatonin   总被引：5，自引：0，他引：5  

F Anton-Tay  C Chou  S Anton  R J Wurtman 《Science (New York, N.Y.)》1968,162(850):277-278

The intraperitoneal administration of melatonin to rats caused an increase in brain serotonin concentration, especially in the midbrain. This effect could be demonstrated within 20 minutes of melatonin administration and was not associated with changes in norepinephrine concentration.  相似文献