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991.
Sherril L. Green Christopher B. Little John D. Baird Robert R. M. Tremblay Laura L. Smith-Maxie 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(2):128-132
The case records of 20 horses with tetanus referred to the Ontario Veterinary College-Veterinary Teaching Hospital between 1970 and 1990 were reviewed. The fatality rate was 75%. There was a strong association with previous vaccination and survival ( P = .03). Most of the animals had been injured an average of 9 days (range 2 to 21 days) prior to development of clinical signs. Hyperesthesia and prolapse of the third eyelid were the most common clinical signs. Treatment regimens varied during hospitalization; however, all horses received parenteral penicillin, tranquilizers, tetanus toxoid, and antitoxin. Five of the nonsurviving animals were given intrathecal tetanus antitoxin. One animal had seizures as a complication of intrathecal treatment. The prognosis was best for horses that (1) had been vaccinated prior to the injury, (2) responded to the phenothiazine tranquilizers, and (3) did not rapidly (over 24 to 48 hours) become recumbent. Considering the species susceptibility, potential for contaminated wounds, and the increased survival of vaccinated horses, yearly revaccination is recommended. 相似文献
992.
Christopher R. Lamb MA VetMB Lawrence J. Kleine DVM MS Majorie C. McMillan DVM 《Veterinary radiology & ultrasound》1991,32(5):211-220
A wide variety of normal and pathologic factors may induce intraabdominal calcification. In general, the most reliable indication of the cause of a calcification is its location; therefore, if the affected organ can be identified the radiographic diagnosis is often straightforward or, at least, limited to relatively few possibilities. With this principle in mind, a series of patients with abdominal calcification are described for the purpose of illustrating the appearance of calcification of various abdominal organs. In addition, etiology for the calcification in each patient is discussed. Certain extraabdominal calcifications which may be seen on abdominal radiographs are also mentioned. 相似文献
993.
ABSTRACT Rhizomania disease of sugar beet represents a major economic threat to the sugar industry in the United Kingdom. Here we use the UK rhizomania epidemic as an exemplar of a range of highly infectious spatially heterogeneous diseases. Using a spatially explicit stochastic model, we investigated the efficacy of a spectrum of possible control strategies, both locally reactive and national in character. These include the use of novel cultivars of beet with different responses to infection, changes in cultivation practice, and reactive containment policies at the farm scale. We show that strictly local responses, including a containment policy similar to that initially implemented in the United Kingdom in response to the disease, are largely ineffective in slowing the spread because they fail to match the natural scale of the epidemic. Larger spatial-scale processes are considerably more successful. We conclude that epidemics have intrinsic temporal and spatial scales that must be matched by any control strategy if it is to be both effective and efficient. We have generated probability distributions for the proportion of farms symptomatic. Over the course of the epidemic, such distributions develop a bimodality that we hypothesize to correspond to the matching of spatial heterogeneity in the susceptible population to the intrinsic scales of the epidemic. 相似文献
994.
Judith Hübschen Lilo Kling Ulrike Ipach Volker Zinkernagel Derek Brown Roy Neilson 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(9):883-891
The nematode species Longidorus attenuatus, L. elongatus, L. macrosoma and Paralongidorus maximusare economically important pests to the viticulture industry due to their ability to vector two nepoviruses (Raspberry Ringspot Virus and Tomato Black Ring Virus) to grapevines. In Germany, these species occur in vineyard soil with other non-vector but morphologically similar longidorid species, L. helveticus, L.profundorum and L. sturhani. Species-specific primers were designed from ribosomal DNA for all seven species to facilitate taxonomic identification for non-specialists. Primers were assessed for their reliability by screening, where possible, a number of populations of each species. Furthermore, their selectivity and sensitivity were determined when challenged with closely related longidorid species and general nematode communities typical of vineyard soil. A multiplex approach using a common forward primer combined with species-specific reverse primers enabled three target nematode species to be detected in the same PCR reaction. All primers were highly specific, detecting all nematode developmental forms from disparate populations and were sufficiently sensitive to detect a single target nematode within a whole nematode community typical of a vineyard soil comprising of a range of non-target species. Given their specificity, sensitivity and reliability, these diagnostic primers should be of great benefit to both phytosanitary/quarantine services related to the viticulture industry and also as a decision management tool for growers. 相似文献
995.
Antonio Ippolito Leonardo Schena Franco Nigro Vincenza Soleti ligorio Thaer Yaseen 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(8):833-843
Two primers, specific for Phytophthora nicotianae (Pn6) and P. citrophthora (Pc2B), were modified to obtain Scorpion primers for real-time identification and detection of both pathogens in citrus nursery soils and roots. Multiplex PCR with dual-labelled fluorogenic probes allowed concurrent identification of both species ofPhytophthora among 150 fungal isolates, including 14 species of Phytophthora. Using P. nicotianaespecific primers a delayed and lower fluorescence increase was also obtained from P. cactorumDNA. However, in separate real-time amplifications, the aspecific increase of fluorescence from P. cactorum was avoided by increasing the annealing temperature. In multiplex PCR, with a series of 10-fold DNA dilutions, the detection limit was 10 pg l-1 for P. nicotianaeand 100 pg l–1 for P. citrophthora, whereas in separate reaction DNA up to 1 pg l-1 was detected for both pathogens.Simple and rapid procedures for direct DNA extraction from soil and roots were utilised to yield DNA whose purity and quality was suitable for PCR assays. By combining these protocols with a double amplification (nested Scorpion-PCR) using primers Ph2-ITS4 amplifying DNA from the main Phytophthora species (first round) and PnB5-Pn6 Scorpion and Pc2B Scorpion-Pc7 (second round), it was possible to achieve real-time detection of P. nicotianaeand P. citrophthora from roots and soil. The degree of sensitivity was similar to that of traditional detection methods based on the use of selective media. The analyses of artificially and naturally infested soil showed a high and significant correlation between the concentration of pathogen propagules and the real-time PCR cycle threshold. 相似文献
996.
997.
Dobson CM 《Science (New York, N.Y.)》2004,304(5675):1259-1262
998.
Cebra CK Tornquist SJ Jester RM Stelletta C 《American journal of veterinary research》2004,65(7):1002-1005
OBJECTIVE: To evaluate the effects of administration of hydrocortisone on plasma concentration of insulin and serum concentrations of glucose, triglyceride, and nonesterified fatty acids (NEFAs) in llamas before and after feed restriction. ANIMALS: 9 adult female llamas. PROCEDURE: Feed was withheld from llamas for 8 hours. Blood samples were collected before (0 minutes) and 120, 180, 240, and 300 minutes after IV injection of hydrocortisone sodium succinate (1 mg/kg) for determination of plasma insulin concentration and serum concentrations of glucose, triglyceride, and NEFAs. The llamas were then fed a limited diet (grass hay, 0.25% of body weight daily) for 21 days, after which the experimental procedures were repeated. RESULTS: Compared with llamas that were not feed-restricted, llamas after feed restriction had significantly higher plasma insulin concentration and serum concentrations of triglycerides and NEFAs. Feed-restricted llamas after hydrocortisone injection had a significantly smaller increase in serum glucose concentration, a decrease (rather than an increase) in serum concentration of NEFAs, and no change in blood concentrations of insulin or triglycerides. CONCLUSIONS AND CLINICAL RELEVANCE: Short-acting glucocorticoid hormones did not appear to increase blood lipid concentrations in healthy llamas, regardless of ongoing fat mobilization. Thus, these hormones appear unlikely to be major direct contributors to diseases such as hepatic lipidosis or hyperlipemia. Although administration of hydrocortisone reduced serum concentration of fatty acids in feed-restricted llamas, its use has not been evaluated in sick camelids and cannot be considered therapeutically useful. 相似文献
999.
Adin CA Farese JP Cross AR Provitola MK Davidson JS Jankunas H 《American journal of veterinary research》2004,65(3):283-288
OBJECTIVE: To describe a percutaneously controlled static hydraulic urethral sphincter (SHUS) and evaluate urodynamic effects of the SHUS in canine cadavers. SAMPLE POPULATION: Cadavers of 6 adult female dogs. PROCEDURE: Cadavers were obtained immediately after dogs were euthanatized. Baseline maximal urethral closure pressure (MUCP) and cystourethral leak point pressure (CLPP) were measured by use of a urethral pressure profilometer. An SHUS system was constructed by use of a silicone vascular occluder and subcutaneous infusion port. The SHUS system was then placed around the pelvic urethra in each cadaver. Measurements of MUCP and CLPP were repeated after varying occlusion of the SHUS (0%, 25%, and 50% occlusion). Baseline MUCP and CLPP values were compared with values obtained at 0%, 25%, and 50% occlusion of the SHUS by use of repeated-measures ANOVA. RESULTS: Mean +/- SD MUCP for canine cadavers was 7 +/- 1.3 cm H2O at baseline, which increased to 127 +/- 53 cm H2O after 50% occlusion of the SHUS. Mean CLPP was 11 +/- 8.6 cm H2O at baseline, which increased to 73 +/- 38 cm H2O after 50% occlusion of the SHUS. Mean MUCP and CLPP were significantly associated with the amount of occlusion. CONCLUSIONS AND CLINICAL RELEVANCE: The SHUS had positive effects on MUCP and CLPP in canine cadavers. Therefore, additional evaluation of the SHUS in live dogs is warranted. 相似文献
1000.
Archer DC Freeman DE Doyle AJ Proudman CJ Edwards GB 《Journal of the American Veterinary Medical Association》2004,224(4):562-564
OBJECTIVE: To determine whether there was an association between a history of cribbing and epiploic foramen entrapment (EFE) of the small intestine in horses. DESIGN: Retrospective study. ANIMALS: 68 horses examined at the University of Illinois or the University of Liverpool veterinary teaching hospitals. PROCEDURE: For horses examined at the University of Illinois that underwent surgery because of strangulating small intestine lesions, information about cribbing was obtained through telephone calls with owners. For horses examined at the University of Liverpool that underwent surgery for colic for any reason, information about cribbing was obtained through a preoperative questionnaire. RESULTS: 13 of 19 (68%) horses with EFE examined at the University of Illinois had a history of cribbing, compared with only 2 of 34 (6%) horses with other strangulating small intestine lesions (odds ratio, 34.7; 95% confidence interval, 6.2 to 194.6). Similarly, 24 of 49 (49%) horses with EFE examined at the University of Liverpool had a history of cribbing, compared with 72 of 687 (10.5%) horses with colic caused by other lesions (odds ratio, 8.2; 95% confidence interval, 4.5 to 15.1). CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that there may be an association between cribbing and EFE in horses, with horses with a history of cribbing more likely to have EFE than horses without such a history. 相似文献