Peptidases of Trichobilharzia regenti (Schistosomatidae) and its molluscan host Radix peregra S. Lat. (Lymnaeidae): construction and screening of cDNA library from intramolluscan stages of the parasite     

Dolecková K  Kasný M  Mikes L  Mutapi F  Stack C  Mountford AP  Horák P 《Folia parasitologica》2007,54(2):94-98

Trichobilharzia regenti is a neurotropic bird schistosome,causing cercarial dermatitis in humans. In this study, ZAP cDNA expression library from Radix peregra s. lat. hepatopancreases containing intramolluscan stages of T. regenti was constructed and screened using PCR with specific and degenerate primers, designed according to previously described serine and cysteine peptidases of other parasite species. Full-length sequences of cathepsins B1 and L, and two serine peptidases, named RpSP1 and RpSP2, were obtained.The protein-protein BLAST analysis and parallel control reactions with template from hepatopancreases of T. regenti non-infected snails revealed that only cathepsin B1 was of parasite origin. The remaining sequences were derived from the snail intermediate host, which implies that the initial source of parasite mRNA was contaminated by snail tissue.Regardless of this contamination, the cDNA library remains an excellent molecular tool for detection and identification of bioactive molecules in T. regenti cercariae.  相似文献   

Vertical variations in wood basic density for two softwood species     

Billard  Antoine  Bauer  Rodolphe  Mothe  Frédéric  Colin  Francis  Christine  Deleuze  Longuetaud  Fleur 《European Journal of Forest Research》2021,140(6):1401-1416

European Journal of Forest Research - Studies on wood basic density (BD) vertical variations become essential to predict more accurately the within-stem distributions of biomass and wood quality in...  相似文献   

Book review and notices     

M. Beveridge  M. Jobling  Colin E. Adams  R. Williamson 《Aquaculture International》1996,4(2):183-188

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Risk factors for bovine mastitis in the Central Province of Sri Lanka     

Suraj Gunawardana  Dulari Thilakarathne  Indra S. Abegunawardana  Preeni Abeynayake  Colin Robertson  Craig Stephen 《Tropical animal health and production》2014,46(7):1105-1112

A study of the risk factors associated with mastitis in Sri Lankan dairy cattle was conducted to inform risk reduction activities to improve the quality and quantity of milk production and dairy farmer income. A cross-sectional survey of randomly selected dairy farms was undertaken to investigate 12 cow and 39 herd level and management risk factors in the Central Province. The farm level prevalence of mastitis (clinical and subclinical) was 48 %, similar to what has been found elsewhere in South and Southeast Asia. Five cow level variables, three herd level variables, and eight management variables remained significant (p?相似文献   

Prion protein genotypes of sheep as determined from 3343 samples submitted from Ontario and other provinces of Canada from 2005 to 2012     

Colin Cameron  Patricia Bell-Rogers  Rebeccah McDowall  Ana R. Rebelo  Hugh Y. Cai 《Canadian journal of veterinary research》2014,78(4):260-266

This study analyzed sheep prion protein (PrP) genotypes of samples submitted from Ontario and other provinces of Canada to the Animal Health Laboratory at the University of Guelph, Guelph, Ontario, between 2005 and 2012. In Ontario, the proportion of scrapie-resistant sheep increased from 2005 to 2012 as evidenced by an increase in the ARR haplotype. When Canadian provinces (Alberta, Ontario, Quebec, and Nova Scotia) were compared from 2008 to 2012, a high proportion of scrapie-resistant sheep was found in all the provinces. The proportions of resistant sheep were lower in Alberta and Quebec than in Ontario and Nova Scotia. Alberta had higher proportions of susceptible sheep and a higher frequency of VRQ alleles, and Quebec had a higher frequency of the ARQ allele.  相似文献   

A B-cell targeting virus disrupts potentially protective genomic methylation patterns in lymphoid tissue by increasing global 5-hydroxymethylcytosine levels     

Nick A Ciccone  William Mwangi  Alexey Ruzov  Lorraine P Smith  Colin Butter  Venugopal Nair 《Veterinary research》2014,45(1)

The mechanisms by which viruses modulate the immune system include changes in host genomic methylation. 5-hydroxymethylcytosine (5hmC) is the catalytic product of the Tet (Ten-11 translocation) family of enzymes and may serve as an intermediate of DNA demethylation. Recent reports suggest that 5hmC may confer consequences on cellular events including the pathogenesis of disease; in order to explore this possibility further we investigated both 5-methylcytosine (5mC) and 5hmC levels in healthy and diseased chicken bursas of Fabricius. We discovered that embryonic B-cells have high 5mC content while 5hmC decreases during bursa development. We propose that a high 5mC level protects from the mutagenic activity of the B-cell antibody diversifying enzyme activation induced deaminase (AID). In support of this view, AID mRNA increases significantly within the developing bursa from embryonic to post hatch stages while mRNAs that encode Tet family members 1 and 2 reduce over the same period. Moreover, our data revealed that infectious bursal disease virus (IBDV) disrupts this genomic methylation pattern causing a global increase in 5hmC levels in a mechanism that may involve increased Tet 1 and 2 mRNAs. To our knowledge this is the first time that a viral infection has been observed to cause global increases in genomic 5hmC within infected host tissues, underlining a mechanism that may involve the induction of B-cell genomic instability and cell death to facilitate viral egress.  相似文献   

Fishing gear effects and ecology of the sea whip (Halipteris willemoesi (Cnidaria: Octocorallia: Pennatulacea)) in British Columbia,Canada: preliminary observations     

Peter M. Troffe  Colin D. Levings  G. E. Piercey  Victor Keong 《水产资源保护:海洋与淡水生态系统》2005,15(5):523-533

• 1. We conducted a preliminary study of the effects of a shrimp beam trawl and prawn traps on sea whips (Halipteris willemoesi (Cnidaria: Octocorallia: Pennatulacea)) at two bays on Clio Channel, south central coast of British Columbia, Canada. Video and grabs surveys were conducted to estimate abundance and make observations on sea whips and their habitat.
• 2. No adult sea whips were caught in six beam trawls. In 600 prawn (Pandalus platyceros) trap sets at Turnour Bay, 30 sea whips were found entangled in the gear and of these 50% of the colonies were damaged. The length of broken sea whips ranged from 0.09 to 1.03 m. At Turnour Bay, the density of adults observed in video surveys ranged from 7.1 to 14.3 m^?2. Juvenile density in grab samples ranged from 53 to 123 m^?2 and a few adults were also caught. The lengths of intact adult sea whips caught in traps and grabs ranged from 0.20 to 1.98 m and juveniles from 0.3 to 0.7 cm.
• 3. Fewer adult sea whips (<0.1 m^?2) were seen in Bones Bay, where sediments were muddier (grain size <0.25 mm: 84.6 to 97.2%) relative to Turnour Bay (grain size <0.25 mm: 71.4 to 85.9%). Bottom water characteristics at the two bays were similar. Seasonal temperature range was 7.5–8.4°C, salinity 30.63–31.49 psu, and dissolved oxygen 2.76–5.97 mg L^?1. Differences in sediment characteristics and food availability may have been a factor influencing differences in abundance of sea whips between the two bays.
• 4. Further data are needed to investigate the effects of trawling and trapping on sea whips, as sample sizes were small in the present study and only two gear types were evaluated. Studies of effects of otter trawling and heavier traps, such as those used for Dungeness crab (Cancer magister), are warranted.

Copyright © 2005 John Wiley & Sons, Ltd.  相似文献   

The ecology of the fish pond ecosystem—with special reference to Africa : Guy Delincé. Developments in Hydrobiology 72. Kluwer Acad. Publ., Dordrecht, 1992. xviii + 230 pp., Price Dfl. 250.00/US$149.00/UK£87.00, ISBN 0-79-231628-2     

Colin E. Nash 《Aquaculture (Amsterdam, Netherlands)》1993,110(3-4):375-376

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Oxidation of glutathione during hydroperoxide metabolism in isolated hepatocytes of rainbow trout (Salmo gairdneri)     

J. Gordon Bell  Colin B. Cowey 《Fish physiology and biochemistry》1990,8(1):11-17

Freshly isolated rainbow trout hepatocytes were exposed to tert-butyl hydroperoxide (BuOOH), a substrate for glutathione peroxidase. BuOOH at a concentration approximately equimolar (1 mM) with intracellular reduced glutathione (GSH) caused a reversible increase in intracellular glutathione disulphide (GSSG) but did not compromise cell viability or damage membrane lipids. BuOOH at 10 mM caused a large irreversible increase in intracellular GSSG followed by efflux into the medium. Considerable leakage of lactate dehydrogenase and loss of highly unsaturated fatty acids, particularly docosahexaenoic acid also occurred. Dependence of hydroperoxide removal on flux through the hexose monophosphate pathway was suggested by the increased release of ¹⁴CO₂ from [1-¹⁴C] glucose from hepatocytes incubated with BuOOH.  相似文献   

British freshwater fishes — the species and their distribution,edited by Cynthia Davies,Jonathan Shelley,Paul Harding,Ian Mclean,Ross Gardiner and Graeme Peirson. Harley Books,Colchester, 2004, 184pp. Price: £25.00. ISBN 0‐946‐58976‐3     

Colin Adams 《水产资源保护:海洋与淡水生态系统》2006,16(2):217-217

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